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166 results about "Clone cell" patented technology
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Group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes; clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence.
Method of cloning reproductive and respiratory syndrome resisting pig
Owner:CHINA AGRI UNIV
Recombinant vector for knock-in of human Huntington gene, construction method of recombinant vector and application of recombinant vector in construction of model pig
ActiveCN107988256AIncreased probability of knock-in positive clonesEfficient FeasibilityStable introduction of DNANucleic acid vectorHuman studiesExon
The invention discloses a recombinant vector for the knock-in of a human Huntington gene, a construction method of the recombinant vector and application of the recombinant vector in the constructionof a model pig. According to the recombinant vector, a human mutated Huntington exon gene is knocked in a fixed point manner for the first time, a virulence gene is knocked in by virtue of a CRISPR / Cas9 technique for the first time, and a donor vector is optimized by optimizing sfRNA, so that the probability that the gene is knocked into a positive cloned cell is increased; and by combining with apig cell nucleus transplantation technique, the probability that a directly obtained positive cell is knocked into the pig is increased, and a small human Huntington gene knock-in pig is obtained, thereby proving the efficient feasibility of the method for constructing gene modified pigs. The constructed Huntington gene knock-in model pig has behavioral characteristics such as respiratory disturbance and dyskinesia similar to human Huntington diseases, and stable heritable passage can be realized, so that a reliable model is provided for the research of the human Huntington diseases; and thenumber can be guaranteed so as to realize drug screening, gene treatment, stem cell treatment and the like, and the model can be a good human disease model.
Owner:JINAN UNIVERSITY +1
Method providing equipment and data safety service for mobile phone users
InactiveCN101132557AImprove securityImprove reliabilityRadio/inductive link selection arrangementsWireless commuication servicesWireless mobile devicesUser authentication
This invention relates to a method for providing devices and data safety service to cell phone users, which utilizes a specific Website to provide individual information backup and backup information resuming function for cell phone users with the function of connecting with Internet server by a method of online managing cell phone IMEI numbers and provides other information service for users of cell phones, which can prevent that individual privacy is leaked and important files can't be found out after a terminal is lost and limit illegal usage of lost cell phones to realize after service mode to enable users and cell phones to negotiate in plant directly or stop the existence of cloned cell phones.
Owner:赵颜
Preparation method of immortalization pig liver cell
InactiveCN1869205AProliferate fastNot tumorigenicForeign genetic material cellsBiotechnologyPork Liver
This invention relates to pork liver cell immortalities and its producing method. Procedures are showed: high live ratio fresh original pork liver cell is got by dispase-collagenase perfusion method; it is cultured for 24 hours. Then cell upper heat removing of recombination retronituse that contains SV40 big T antigen is used to infect the pork cell under condition of polybrene which concentration is 8ug / ml. Then it is medicine pressure filtrated by 500ug / ml G418 after one week, single clone cell is picked for large culture when the cell clone appears and grows to 1.0-2.0cm to get pork cell that can passage. Reinfection is done under condition of 8ug / ml polybrene. Then it is medicine pressure filtrated by 2ug / ml puromycin after one week. Single clone cell is picked for large culture when the cell clone appears and grows to 1.0-2.0cm to get immortality pork cell.
Owner:ZHEJIANG UNIV
Method for obtaining transgenic bovine fetal fibroblast by using Cas9 cutting nuclease-mediated Ipr1 fixed point insertion
InactiveCN107619837AAvoid loss of activityAvoid lossGenetically modified cellsMicrobiological testing/measurementEmbryoUterus
The invention discloses a method for obtaining transgenic bovine fetal fibroblast by using Cas9 cutting nuclease-mediated Ipr1 fixed point insertion. The bovine fetal fibroblast is cotransfected by adonor vector and a CRISPR / Cas0 expression vector for a target locus through an electroporation method, an MSR1 promoter in the donor vector can realize specific expression in full-time phagocytic cells, and after puromycin drug screening, the targeted positive clone cell is obtained through PCR authentication. The transgenic positive clone cell is used as a donor cell so as to obtain a transgenicclone embryo, the transgenic clone embryo is further transplanted into the uterus of a rutting acceptor calf, and finally a living Ipr1 fixed point insertion transgenic cloned calf is obtained.
Owner:NORTHWEST A & F UNIV
Interleukin-15 gene modified natural killing cell strain and its preparation method
ActiveCN1493687ASmall doseIncrease lethalityVector-based foreign material introductionForeign genetic material cellsWhite blood cellCulture mediums
An interleukin-15 (IL-15) gene modified natural killing cell strain for immunotherapy of tumor is prepared through inserting the cDNA coding region of IL-15 in pcDNA3 eucaryotic expression carrier, configuring secretion-type recombinant eucaryotic expression carrier pc DNA3 / IL-15, using liposom to transfecte cell NK-92, adding Geneticin (G418) to screening culture medium, screening, limited dilution to cloned cells, detecting activity, choosing positive cell clones, and amplification.
Owner:UNIV OF SCI & TECH OF CHINA +1
Genomic engineering of pluripotent cells
ActiveUS20180155717A1Improve durabilityImprove the immunityHydrolasesStable introduction of DNAGenomic engineeringPolynucleotide
Provided are methods and compositions for obtaining genome-engineered iPSCs, and derivative cells with stable and functional genome editing at selected sites. Also provided are cell populations or clonal cell lines derived from genome-engineered iPSCs, which comprise targeted integration of one or more exogenous polynucleotides, and / or in / dels in one or more selected endogenous genes.
Owner:FATE THERAPEUTICS
Method for Cloning T Cell Receptor
InactiveUS20150203886A1Raise ratioLow frequencyImmunoglobulin superfamilyBlood/immune system cellsTcr repertoireAntigen specific
An object is to provide a TCR closing system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR α / β cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A. According to the present invention, a target TCR gene can be cloned within a shorter time compared with that repaired by the conventional methods, for example, about ten days. Further, according to the present invention, genes of TCR α chain and β chain can be highly efficiently cloned. Under the conditions of the examples, a pair of a TCR α chain and TCR β chain could be obtained from stimulated T cells sorted as single cells at a ratio of 100%.
Owner:UNIVERSITY OF TOYAMA
Genomic engineering of pluripotent cells
ActiveUS10287606B2Improve durabilityImprove the immunityHydrolasesStable introduction of DNAGenomic engineeringPolynucleotide
Owner:FATE THERAPEUTICS
Melamine and carrier protein couplet product, preparation method and uses of melamine antibody
InactiveCN101429243AEfficient detectionEasy to operateSerum immunoglobulinsOvalbuminBALB/cCarrier protein
The invention discloses a preparation method and application of a product obtained by coupling melamine with carrier protein, as well as a melamine antibody. The product obtained by coupling the carrier protein with the melamine is used as artificial antigen and applied to an immunological method for melamine detection. The preparation method comprises the following steps of immunizing animals with the coupled product so as to prepare an antibody used for melamine detection, fusing BALB / C mouse spleen cells immunized with the coupled product and SP2 / 0 mouse myeloma cells, obtaining hybridoma capable of stably transferring culture and secreting anti-melamine specific monoclonal antibodies by screening positive hybridoma and cloning cells, and preparing an ascites monoclonal antibody. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the melamine, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling melamine with carrier protein, as well as the melamine antibody provides service for the rapid detection of melamine-type residue in foods.
Owner:ZHEJIANG UNIV
Genomic engineering of pluripotent cells
ActiveUS20190271005A1Increase resistanceMaintain pluripotencyHydrolasesAntineoplastic agentsGenomic engineeringPolynucleotide
Provided are methods and compositions for obtaining genome-engineered iPSCs, and derivative cells with stable and functional genome editing at selected sites. Also provided are cell populations or clonal cell lines derived from genome-engineered iPSCs, which comprise targeted integration of one or more exogenous polynucleotides, and / or in / dels in one or more selected endogenous genes.
Owner:FATE THERAPEUTICS
B cell-derived ips cells and application thereof
InactiveUS20110231944A1Low costLow cost productionGenetically modified cellsArtificial cell constructsMonoclonal antibodyImmunodeficient Mouse
Provided are a B cell-derived iPS cell generated using a convenient technique, a technology for providing a human antibody at low cost using the iPS cell, an immunologically humanized mouse prepared using cells differentiated from the iPS cell, and the like. Also provided are a cloned cell obtained by contacting a B cell with nuclear reprogramming factors excluding C / EBPα and Pax5 expression inhibiting substances, particularly nucleic acids that encode Oct3 / 4, Sox2, Klf4 and c-Myc, wherein the cloned cell has an immunoglobulin gene rearranged therein and possesses pluripotency and replication competence (B-iPS cell). Still also provided are a method of producing a monoclonal antibody against a specified antigen, comprising recovering an antibody from a culture of B cells obtained by differentiating a B-iPS cell derived from a B cell immunized with the specified antigen, and a method of generating an immunologically humanized mouse, comprising transplanting to an immunodeficient mouse a human immunohematological system cell obtained by differentiating a B-iPS cell.
Owner:RIKEN
Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process
InactiveUS20190024079A1Improve proliferative abilityReplication capacity can be improvedHydrolasesGenetically modified cellsTelomeraseADAMTS Proteins
Owner:UPSIDE FOODS INC
Targeting vector and reconstituted cell for Cas9-mediated site-specific integration of FABP4 (adipose fatty acid-binding protein) gene and MSTN (myostatin) gene point mutation
PendingCN109112159AShorten breeding timeImprove transgenic efficiencyGenetically modified cellsStable introduction of DNAMyostatinBovine oocyte
The invention discloses a targeting vector and a reconstituted cell for the Cas9-mediated site-specific integration of FABP4 (adipose fatty acid-binding protein) gene and MSTN (myostatin) gene point mutation. The invention utilizes an electroporation method to co-transfect a Cas9 eukaryotic expression vector and a targeting vector targeting the MSTN gene to Luxi bovine fetal fibroblasts, consequently, gene targeting for the Luxi bovine fetal fibroblasts is realized, and bovine fetal fibroblasts with FABP4 gene and MSTN gene point mutation knocked in the MSTN site are obtained. By Junction of PCR (Polymerase Chain Reaction) screening and verification, targeted positive cloned cells are obtained. The positive cloned cells as donor cells are transplanted into denucleated bovine oocytes, so that a transgenic cloned embryo can be obtained, and thereby a solid foundation is laid for the rapid and efficient development of high-quality new genetically modified beef varieties.
Owner:NORTHWEST A & F UNIV
Method for screening monoclonal antibodies on different binding sites of antigen
ActiveCN104020289ARapid determinationSimplify the screening stepsBiological material analysisImmunoglobulins against cell receptors/antigens/surface-determinantsBinding siteAntigen binding
The invention discloses a method for screening monoclonal antibodies on different antigen binding sites. The method comprises the following steps: preparing an antigen specific monoclonal antibody by an antigen immune animal with two or more antigenic determinants through a hybridoma monoclonal technology; carrying out in-vitro cell culture on positive clone cell strains to obtain cell culture liquid supernatant containing the antigen specific monoclonal antibody; adding the hybridoma cell culture liquid supernatant into a solution of rubber latex grains which are covalently cross-linked with protein A or G in a two-and-two combined manner; and incubating and adding antigens to obtain a monoclonal antibody set with the increased light absorbance, namely obtain the monoclonal antibody with the different antigen binding sites.
Owner:NINGBO ACCUTECH BIOSCI LTD
A549 nude mouse model of stably expressed luciferase and building and application thereof
InactiveCN102936600AObservation is intuitive and convenientReliable resultsGenetic engineeringFermentationWilms' tumorLentivirus Infections
The invention relates to an A549 nude mouse model of stably expressed luciferase and building and application thereof. The nude mouse model leads firefly luciferase genes to human lung adenocarcinoma A549 cell strains through the gene recombination technology and lentivirus infection, tumor clone cell strains of the stably expressed luciferase are obtained through subcloning screening, and recombinational A549 cells are inoculated in a mouse to obtain the A549 nude mouse model. Compared with a traditional tumor model drug effect detection method, the A549 nude mouse model enables observation to be visual and convenient, can be used for somatoscopy without damaging animals, is reliable in result, and has good application prospect.
Owner:SUZHOU RES INST OF TONGJI UNIV
Development of clone of domestic animal used as mammary gland boireactor by means of Cre-LoxP site-specific targeting somatic cell
InactiveCN1502694AEfficient preparationOther foreign material introduction processesFermentationBiotechnologyFibroblast
The present invention discloses a method for preparing clonal livestock as mammary gland bioreactor by using Cre-LoxP to make site-speciofic target practice of body cell. Said method includes the following steps: (a). site-specific introducing the constituent containing LoxP sequence into fibroblast; (b). screening LoxP sequence site-specific integrated cell; (c). making target practive carrier containing exogenous gene and Cre expression plasmid / Cre protein cotransfect LoxP sequene site-specific integrated cell so as to screen and obtain the cell of site-specific integrated exogenous gene; and (d). reforming clonal animal. Said invention also provides correspondent constituent and clonal cell.
Owner:SHANGHAI GENON BIOENG
Method for improving chip yields in the presence of via flaring
InactiveUS6904575B2Reduce errorsComputer aided designSoftware simulation/interpretation/emulationCrop yieldIntegrated circuit
The current invention provides a modification procedure that reduces errors in integrated circuits due to via shorts while at the same time avoiding the unnesting of the layout design and thereby permitting verification of the layout design by LVS testing tools. The current invention identifies if potentially shorting vias have electrically redundant paths and, if so, creates cloned cells of the original cell but void of the potentially shorting vias. The cloned cell is electrically comparable to the original cell. In addition, each instantiation of the original cell in the shapes data base is replaced with the cloned cell when electrical redundancy is present. Also, the number of vias removed can be minimized or maximized while, at the same time, all via electrical shorts are removed, depending on the design requirements.
Owner:IBM CORP
Efficient screening method of exogenous protein expression cell strain
InactiveCN104404082AReduce workloadReduce build timeVector-based foreign material introductionTransfer cellScreening method
The invention belongs to the technical field of cell engineering, and particularly relates to an efficient screening method of an exogenous protein expression cell strain. The efficient screening method of the exogenous protein expression cell strain disclosed by the invention comprises the following steps: using an interest protein expression vector to transfect host cells; pressurizing a screening drug, and forming a stable cell pool; inoculating a semisolid culture medium with the cells in the stable cell pool; transferring the cloned cells from the semisolid culture medium to a 96-hole cell plate to be continuously cultured for 4-5 days, and detecting the interest protein expression index in the cell supernatant; transferring cells which are first 50 in the ranking of expression index to a 24-hole plate to be continuously cultured for 5 days; further transferring the cells in the 24-hole plate to a 6-hole to be continuously cultured for 5 days; transferring the cells in the 6-hole plate to a shake flask for culture, and detecting and evaluating the interest protein expression indexes of different cloned cells under a suspension state; according to the evaluation result in the shake flask, performing a stability passage test on the cells which are first 10 in the ranking of the expression index; according to the interest protein expression level of the cell strain and the stability of the continued expression protein, determining the candidate cell strains.
Owner:SHANGHAI MBR BIOMEDICAL TECH
Hybridoma cell strain capable of secreting fluridone monoclonal antibody as well as preparation method and application of hybridoma cell strain
ActiveCN113684187AEfficient and stable secretionHigh sensitivityOvalbuminSerum albuminImmune profilingMicroorganism
The invention relates to a hybridoma cell strain secreting a fluridone monoclonal antibody. The hybridoma cell strain is named as a monoclonal cell strain LKS, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 13, 2021, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 22324. The hybridoma cell strain disclosed by the invention can efficiently and stably secrete the fluridone monoclonal antibody and has relatively good sensitivity and specificity when being applied to immunoassay detection of fluridone, the IC50 value is 0.86 ng / mL, and the cross-over rate of fluridone analogues is less than 10%.
Owner:JIANGNAN UNIV
Method for constructing CHO cell strain for stably and efficiently expressing human serum albumin and interleukin II fusion protein
The invention discloses a method for constructing a CHO cell strain for stably and efficiently expressing a human serum albumin and an interleukin II fusion protein. The CHO monoclonal cell strain for stably and efficiently expressing the human-derived recombinant protein can be obtained by electrically transferring a plasmid with the human serum albumin and the interleukin II fusion gene into the CHO cell. The monoclonal cell strain obtained by the invention is capable of secretory expression of the human serum albumin and the interleukin II fusion protein, the protein expression quantity is high, and the fusion protein can be obtained through the separation and purification; the in vitro biological activities of two fusion proteins are higher than the mol monomer 1L-2 activity. The CHO cell strain can be widely applied to the medicines for treating various diseases such as tumor, hepatitis, pneumonia and immunodeficiency disease.
Owner:JIANGNAN UNIV
Method for preparing and screening cell line expressing bispecific antibody
InactiveCN104818295AFermentationVector-based foreign material introductionBispecific antibodyMaterial resources
The present invention provides a method for preparing and screening a cell line expressing bispecific antibody. The method comprises: constructing two plasmids expressing bispecific antibody, wherein the two plasmids contain double promoters and respectively express different fluorescent proteins; and carrying out transformation, culture and extraction on the two recombinant plasmids, co-transfecting the two recombinant plasmids into host cells, screening the positive monoclonal cell line expressing the double fluorescence, and evaluating the yield and the stability according to the fluorescence intensity. With the method of the present invention, the high yield cell line can be conveniently and rapidly screened in advance, the test period can be shortened, the manpower and material resource investment can be reduced, and the accuracy is high.
Owner:WUHAN YZY BIOPHARMA CO LTD
Cell strain capable of stably expressing Cas9 protein as well as preparation method and application thereof
InactiveCN110951782AAvoid the risks of integrationImprove cutting efficiencyHydrolasesGenetically modified cellsCas9Homomeric
The invention discloses a cell strain capable of stably expressing Cas9 protein as well as a preparation method and an application thereof. According to the method, a Cas9 protein expression gene, anantibiotic selection marker gene and a fluorescence marker gene are introduced into a 293T cell of a tool cell through a CRISPR / Cas9 system; after positive clone cell strains are obtained through resistance and fluorescence screening, the screening marker is cut off through the CRISPR / Cas9 system, and finally the cell strains which can stably express Cas9 protein and do not have any screening marker are obtained. The cell strain constructed by the invention is constructed on the basis of a CRISPR / Cas9 gene editing system and a homologous recombination principle, and is integrated at a safety site-AAVS1 site in the tool cell genome in a fixed point mode; the Cas9 protein can be stably expressed and does not screening marker is obtained, so that the tool cell strain of a subsequent screeningexperiment is not influenced.
Owner:湖南普拉特网络科技有限公司
Method and kit for identifying sensitivity of antibody and clone cell strain
InactiveCN103185803AReduce manpowerReduce material wasteBiological material analysisImmunoglobulinsAntibody adsorptionCell strain
The invention discloses a method for identifying sensitivity of an antibody as well as quality of a clone cell strain in the preparation process of the antibody. The method comprises the following steps: acquiring a solid-phase carrier, cells and the antibody, adsorbing the antibody on the solid-phase carrier, incubating the cells and the antibody adsorbed on the solid-phase carrier, keeping cells that are combined with the antibody, dyeing and counting the cells combined with the antibody, and identifying the sensitivity of the antibody and the quality of the clone cell strain according to the cell counting result. The invention further provides a kit applicable to the method. The method intuitively reflects the level of combination of the antibody and a native conformation antigen, and the identifying method is simple, quick and intuitive.
Owner:SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
Swine testicular clone cell line and production method of classical swine fever live vaccine
ActiveCN102965332AMicroorganism based processesAntiviralsBovine Viral Diarrhea VirusesInfection rate
The invention provides a highly classical swine fever virus infective swine testicular clone cell strain ST-B2, which has a preservation number of CCTCC NO.C2011101, and a preparation method of the swine testicular clone cell. The method comprises the steps of: 1) subjecting a swine testicular cell to limited dilution, conducting cell cloning and subcell cloning, thus obtaining a subcellular clone strain; and 2) selecting a subclone strain of the swine testicular cell with a highest classical swine fever virus infection rate, i.e. the highly infective swine testicular clone cell of the classical swine fever virus. The invention also provides a method for preparation of a high titer classical swine fever virus solution and a classical swine fever live vaccine from the swine testicular clone cell. The swine testicular clone cell provided in the invention has a high classical swine fever virus infection rate, and the classical swine fever vaccine produced from the swine testicular cell line ST clone cell strain ST-B2 by a virus-carrying and virus transmission technique has high virus titer, hard exposure to BVDV (bovine viral diarrhea virus) pollution and good pureness. By the virus-carrying and virus transmission technique, a resurgent cell can undergo continuous passage to at least 15 generations. The cell resurrection and virus inoculation times can be reduced, the production process is simplified, and the production efficiency is improved.
Owner:PU LIKE BIO ENG
Method for knocking out AAV receptor, HEK293 cell strain with AAV receptor knocked out and application
ActiveCN107245475AReduce lossesIncrease final yieldCell receptors/surface-antigens/surface-determinantsVectorsCytologyCell strain
The invention relates to the field of gene engineering and cytology, in particular to an AAV receptor knocking out method, an HEK293 cell strain with an AAV receptor being knocked out and an application. The knocked-out AAV receptor is KIAA0319L. The knocking out method comprises the following steps: establishing a sgRNA carrier of a targeted AAVR genome sequence; carrying out transfection for a 293 T-cell, and culturing to obtain each clonal cell; carrying out Suveyor gene mutation analysis test; selecting AAVR mutant monoclone; then selecting an AAVR mutant cell strain with high yield of AAV; identifying a mutation condition of other potential mutant site; and enlarging the cell strain without other mutant sites, and establishing a library for production. The final yield of the AAV produced by the HEK293 cell strain of the knocked-out AAV receptor is about 50 percent higher than the yield of the conventional HEK293.
Owner:GUANGZHOU PACKGENE BIOTECH CO LTD
Establishment and application of radiotherapy model for in-situ bearing cancer of mouse lung cancer
InactiveCN107723312AObservation is intuitive and convenientReliable resultsGenetically modified cellsNucleic acid vectorAbnormal tissue growthCancer cell
The invention relates to the fields of tumor models and establishment and application of tumor models and particularly relates to an establishment method and application a radiotherapy model for mousein-situ bearing cancer. The radiotherapy model can be used for evaluating radiotherapy effects. The establishment method comprises the steps of introducing firefly luciferase genes into a mouse Louislung cancer cell strain (LLC) by virtue of a gene recombination technique by taking a wild mouse as a carrier, carrying out subcloning screening so as to obtain a tumor cloning cell strain capable ofstably expressing luciferase, and inoculating recombinant LLC cells to a lung of the mouse. Compared with a traditional tumor model method, the establishment method has the advantages that the pathological and physiological environments of the lung cancer can be stimulated; the method has very important significances to the diagnosis and treatment of the lung cancer; and the model contains luciferase genes and can be used for imaging in a small animal living body imaging instrument, the development process of the lung cancer can be dynamically monitored in real time, the observation is relatively visible and convenient, living bodies can be observed without injuring the animals, the result is relatively reliable, and the model has very good application prospects.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Selection of stem cell clones with defined differentiation capabilities
Disclosed are methods for producing a clonal population of cells involving: a) obtaining a population of pluripotent or multipotent cells that have been expanded in vitro and maintained in an undifferentiated or essentially undifferentiated state; b) expanding individualized cells of the population into clonal populations of cells; and c) selecting one or more clonal population of cells determined to have the ability to differentiate into a population that is at least about 50% homogeneous for either neural cell types, hepatocytes, or cardiomyocytes. Also disclosed are clonal populations of cells produced by the methods of the present invention, and methods of treating disease in subjects involving administration of clonal cells of the present invention to a subject. Methods of screening test compounds that involve contacting a test compound with a clonal population of cells produced by the methods of the present invention are also set forth.
Owner:RES DEVMENT FOUND
WPRE mutant virus vector and application thereof
ActiveCN111733187AEasy to buildThe promotion effect is obviousVectorsFermentationNucleotideViral vector
The invention belongs to the field of viral vectors, and particularly relates to a WPRE mutant virus vector and application thereof. The WPRE mutant virus vector includes a WPRE mutant-03, and a nucleotide sequence of the WPRE mutant-03 is shown as SEQ ID NO:3. A construction method include the steps that S1, random mutation target fragments are prepared; S2, the random mutation target fragments are connected to a target vector that can express fluorescent protein; S3, after coating on plate is carried out, cells are screened by flow cytometer; S4, a cell genome obtained by screening is extracted, and a PCR technology is used to amplify a WPRE region; and S5, after a T vector is connected, coating on plate is carried out to obtain cloned cells, sequencing is carried out to obtain the WPREmutant virus vector. A WPRE random point mutation virus library is designed and constructed through a large amount of innovative work, and a novel WPRE mutant virus vector is found through library screening and has a significant promoting effect on virus packaging.
Owner:OBIO TECH SHANGHAI CORP LTD
BHK-21 cell strain and culture method and purpose thereof
InactiveCN108396006AClear ingredientsWide range of usesCulture processArtificial cell constructsSerum free mediaBottle
The invention relates to a method for culturing a BHK-21 cell strain suspendedly grown in a plurality of serum-free mediums. The method comprises the following steps: S1) recovering a BHK-21 cell line; S2) sorting the BHK-21 cell line, and cloning cell and performing culture amplification; and S3) performing serum-free suspension culture on a BHK-21 clone cell colony. The invention also provides the BHK-21 cell strain suspendedly grown in a plurality of serum-free mediums and a purpose thereof. The method does not require a microcarrier as an adhesion carrier, bottle rotation is not required for keeping a rolling culture mode, the medium does not contain serum, and no protein component is contained, and the medium is the CD-grade commercial serum-free medium composed of inorganic salt anda nutriment. The serum-free suspension culture BHK-21 cell strain can directly add DMSO by the serum-free medium for cryopreservation, and the serum-free medium can be directly used for recovery. TheBHK-21 cell strain provides novel thinking and the method for industrial-grade expanded production of a virus vaccine.
Owner:CANVEST WUHAN BIOTECH
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