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Method for screening high quality spirulina princeps strain for large scale production

A technology of spirulina and strains, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of cumbersome procedures, high cost, and heavy workload, and achieve the effect of clear standards and low cost

Inactive Publication Date: 2008-02-06
ZHEJIANG UNIV
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Problems solved by technology

Although this method is practical, the procedure is cumbersome, the workload is heavy, the cycle is long, and the cost is high

Method used

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  • Method for screening high quality spirulina princeps strain for large scale production

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Embodiment Construction

[0010] The technical solution of the present invention can be realized through the following steps

[0011] 1, the selected material is known 7 strains of Arthrospira platensis strains, i.e. Sp-1, Sp-2, Sp-3, Sp-5, Sp-9, Sp-10 and Sp-15 (Zhejiang University Nucleus Agricultural Science The Laboratory of Biological Resources and Molecular Engineering of the Institute is also preserved). Among them, Sp-3, Sp-5, and Sp-15 have strong adaptability to the environment, and are widely used in large-scale production and breeding, while Sp-1, Sp-2, Sp-9, and Sp-10 are poorly adapted to the environment. Cannot be used to produce improved seeds;

[0012] 2. Reagents and instruments: Taq DNA polymerase and agarose are products of Shanghai Sangon Bioengineering Company; the cloning vector pMD18-T, dNTP, DNA restriction endonuclease, and T4 DNA ligase are all products of Japan TaKaRa Company; The gel recovery kit was purchased from Shanghai Yingjun Company; the PCR primers were synthesize...

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Abstract

The method of screening high quality spirulina strain for large scale production includes designing primer of 16S-23S rRNA transcription spacer, cloning and measuring 16S-23S rRNAITS sequences shown in SEQ No. 1 of 7 spirulina strains Sp-1, Sp-2, Sp-3, Sp-5, Sp-9, Sp-10 and Sp-15 by means of PCR technology; separating the 7 spirulina strains into two groups, including the first group comprising Sp-3, Sp-5 and Sp-15, and the second group comprising Sp-1, Sp-2, Sp-9 and Sp-10 according to the similarity of the sequences; and measuring the 16S-23S rRNAITS sequence of the candidate strain and comparing with those of the said strain. When the candidate strain may be classified into the first group, it has excellent character and is suitable for large scale production; when the candidate strain may be classified into the first group, it is not suitable for large scale production.

Description

technical field [0001] The invention belongs to the technology for the development and application of spirulina Background technique [0002] Spirulina is a photosynthetic and oxygen-releasing prokaryotic filamentous microalgae in a regular spiral shape, a genus of Cyanophyta, Oscillatoriales, and Oscillatoriaceae [Wuhan Plant Scientific Research, 1997, 15(4): 369-374], because it is rich in high-quality protein and a variety of biologically active substances, it has received great attention at home and abroad, and has formed a huge Spirulina on the basis of a large number of studies. Industry, and used in food, biomedicine and health care, feed, fine chemicals and other fields. Spirulina platensis in this genus is the most widely used species in commercial breeding production and development at home and abroad. It is worth pointing out that numerous experimental studies and long-term production practices have shown that there are many strains of Spirulina platensis specie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 汪志平杨灵勇陈晓燕李雪斌
Owner ZHEJIANG UNIV
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