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Method of discriminating good and bad production of spirurina strain

A technology for producing traits and spirulina, which is applied in the field of spirulina development and application, and can solve problems such as high cost, heavy workload, and long cycle

Inactive Publication Date: 2012-08-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method is practical, the procedure is cumbersome, the workload is heavy, the cycle is long, and the cost is high

Method used

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  • Method of discriminating good and bad production of spirurina strain

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Embodiment Construction

[0011] Detailed technical scheme of the present invention can be implemented through the following steps:

[0012] 1. Selected materials: 4 strains of Spirulina platensis strains used to construct identification standards are known Sp-1, Sp-2, Sp-3 and Sp-5 (Biological Resources and Molecular Engineering Experiment of Institute of Nuclear Agricultural Sciences, Zhejiang University It is also stored in the room), and it performs well in the laboratory culture tests that can be automatically regulated in the environment such as temperature and light. Among them, Sp-3 and Sp-5 have strong adaptability to the environment and are widely used in large-scale production and breeding; and Sp-1 and Sp-2 could not be used as good production varieties due to their poor adaptability.

[0013] 2. Reagents and instruments selected: Taq DNA polymerase and agarose are products of Shanghai Sangon Bioengineering Company; the cloning vector pMD18-T, dNTP, DNA restriction endonuclease, and T4 DNA ...

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Abstract

The present invention relates to a method for identifying spirulina line production character quality. Said method includes the following steps: selecting four plants of Sp-1, Sp-2, Sp-3 and Sp-5 from abtuse acron spirulina line; utilizing upstream primer PF:5'-CAATACATCTTCG CCGATTT-3' and downstream primer PR: 5'-CGTATTATCGGTAGTCATCGG-3'; in PCR reaction system making reaction: 94 DEG.C 5 min, 94 DEG.C 30s, 58 DEG.C 1 min, 72 DEG.C 2 min, 30 cycles; 72 DEG.C 8 min; connecting PCR product recovered and purified by DNA gel onto PMD18-T carrier to screen male clone; extracting plasmid and making enzyme incision identification, sequencing the recombinant plasmid containing target fragment; making the cpc HID operon gene sequences of above-mentioned four plants and correspondent sequence of identified spirulina plant undergo the process of multiple sequence comparison and creating phylogenetic tree so as to identify spirulina line character quality.

Description

technical field [0001] The invention belongs to the technology for the development and application of spirulina Background technique [0002] Spirulina is a photosynthetic and oxygen-releasing prokaryotic filamentous microalgae in a regular spiral shape, a genus of Cyanophyta, Oscillatoriales, and Oscillatoriaceae [Wuhan Plant Scientific Research, 1997, 15(4): 369-374], because it is rich in high-quality protein and a variety of biologically active substances, it has received great attention at home and abroad, and has formed a huge Spirulina on the basis of a large number of studies. Industry, and used in food, biomedicine and health care, feed, fine chemicals and other fields. Spirulina platensis in this genus is the most widely used species in commercial breeding production and development at home and abroad. It is worth pointing out that numerous experimental studies and long-term production practices have shown that there are many strains of Spirulina platensis specie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 汪志平杨灵勇曹学成黄晖
Owner ZHEJIANG UNIV
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