DNA (Desoxyribonucleic Acid) specimen collection and quality control method for fetus chromosome aneuploid detection

Abstract

The invention discloses a DNA (Desoxyribonucleic Acid) specimen collection and quality control method for fetus chromosome aneuploid detection. The method comprises the following steps: I, performingspecimen collection, namely collecting 5.0mL of peripheral blood of a pregnant woman by using a blood collection tube I, and after collection is completed, slightly inverting the blood collection tube, placing the blood collection tube in a refrigerator in time, collecting 10.0mL of peripheral blood of the pregnant woman by using a blood collection tube II, after collection is completed, slightlyinverting the blood collection tube, and preserving at a normal temperature; II, pre-cooling a low speed centrifuge, putting into the blood collection tube II, centrifuging for minutes, sucking supernate blood plasma, transferring into an EP (Eppendorf) tube on an ice box, and marking with corresponding sample numbers; III, pre-cooling a high speed centrifuge till a temperature is stabilized, putting into the EP tube, centrifuging, sucking the supernate blood plasma on the ice box with a gun head pointing to non-leukocyte precipitation part, sub-packaging into the EP tube on the ice box, transferring into the blood plasma, marking sample numbers and blood plasma tube numbers, and preserving in the refrigerator; IV, performing quality control and treatment on the sample preserved in the step III. The method is definite in standard, good in practice operation effect and efficient and rigorous.

Description

technical field [0001] The invention belongs to the field of non-invasive prenatal screening and detection, and specifically refers to a DNA sample collection and quality control method in the detection of fetal chromosomal aneuploidy. Background technique [0002] The rapid acquisition of genetic information of living organisms is of great significance to the research of life sciences. The reliance of the first-generation sequencer on electrophoretic separation technology makes it difficult to further improve the analysis speed and parallelization degree, and it is also difficult to reduce the sequencing cost through miniaturization. After continuous technical development and improvement, at the beginning of the 21st century, the second-generation sequencing technology marked by Roche454 technology, Illumina's GenomeAnalyzer technology and ABI's Solid technology was born. Second-generation technology has greatly reduced the cost of sequencing, and has also greatly increase...

AI Technical Summary

Problems solved by technology

To solve a DNA sample collection and quality control method in the detection of fetal chromosomal aneuploidy with high cost, unscientific process, unclear standards, poor practical operation effect, high operation difficulty, difficult to copy operation, and non-standard sample collection process , Sample storage and quality control are unqualified, easily lead to sample failure, insufficient sampling volume, incomplete plasma separation, etc., technical problems that affect subsequent testing and the success rate of subsequent links