The invention provides a semi-skimmed trichosanthes kirilowii maxim protein beverage which comprises the following components as per part by weight: 35 to 40 parts of trichosanthes kirilowii maxim seed protoplasm, 6 to 10 parts of cane sugar, 0.15 to 0.2 part of compound emulsion agent, 0.15 to 0.2 part of composite complexing agent, 0.001 to 0.002 part of flavoring agent and the balance of soft water, wherein the pH is adjusted to be 6.5 to 6.8, and the soft water is added to achieve 100 parts. The preparation method comprises the following steps of raw materials selection, shucking, peeling, drying, cold pressing, soaking, pulping, filtration, homogenization, auxiliary material allocation, second homogenization, package and sterilization. The semi-skimmed trichosanthes kirilowii maxim protein beverage has a soft and smooth taste and effects of hairdressing and nourishing, can achieve the functions of invigorating the stomach and moistening the lung, relaxing bowel to relieve constipation, inducing diuresis, loosening the chest, relieving the heart and refreshing the spirit, resisting bacteria and building body after being drunk for a long time, and has a strong inhibiting effecton choriocarcinoma cell proliferation in vitro and Human Immunodeficiency Virus (HIV). The preparation method has scientific, simple and convenient processing technology, and keeps the nutritional ingredient of trichosanthes kirilowii maxim seed furthest; and the prepared semi-skimmed trichosanthes kirilowii maxim protein beverage has a low fat content, a high protein content, and can be drunk toenjoy better health.