Separation and refining process for rice bran polysaccharide

A rice bran polysaccharide, separation and purification technology is applied in the field of separation and purification methods of rice bran polysaccharide to achieve the effects of improving yield, scientific process and low cost

Inactive Publication Date: 2007-07-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Microwave-assisted extraction was used to improve the extraction rate of rice bran polysaccharides, and Q-Sepharose Big Beads anion exchange column chrom

Method used

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  • Separation and refining process for rice bran polysaccharide

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Embodiment 1

[0017] The preparation of embodiment 1 rice bran crude polysaccharide

[0018] Mix rice bran and deionized water according to the ratio of material to liquid 1:10, treat with 400W microwave for 2 minutes, then centrifuge at 3000rpm for 20min, take the supernatant, add α-amylase of pH 6.0 to hydrolyze for 1h, then centrifuge to get the supernatant Add protease at pH 4.5 to remove the protein after 1 h, and continue centrifuging for 30 min after 1 h, add 95% ethanol to the obtained supernatant to precipitate, so that the final ethanol concentration is 60%-90%. The next day, the precipitate was collected by centrifugation, and after dilution, it was dialyzed with a 3500 molecular weight dialysis bag, then rotated to evaporate, vacuum freeze-dried to obtain rice bran crude polysaccharide.

Embodiment 2

[0019] Example 2 Column Chromatography Separation and Purification of Rice Bran Polysaccharide

[0020] The crude polysaccharides from rice bran were eluted by Q-Sepharose Big Beads anion-exchange column chromatography.

[0021] Use 0.1mol / L, pH8 phosphate buffer as the eluent, load 25-30ml of crude polysaccharide sample, and then use 0, 0.3, 0.5 and 0.7mol / L NaCl solution respectively, use 1 column volume, A flow rate of 1.5 mL / min was used for stage elution on a Q-Sepharose Big Beads anion exchange column. 0.5mol / L NaCl solution eluted to get 2 peaks, and other concentration NaCl solutions eluted to get a peak. The P2 fraction obtained by the elution of 0.3mol / L NaCl solution was collected, and the sugar content of each peak was determined by the phenol-sulfuric acid method. In comparison, the elution peak of 0.3mol / L NaCl solution had higher sugar content and lower protein content. The collected P2 fraction was further purified by Sepharose CL-4B gel filtration column lay...

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Abstract

The invention discloses a separating purifying method of rice bran polysaccharide, which comprises the following steps: leaching the raw material through microwave auxiliary water; enzymolyzing to remove starch and protein; condensing; dialyzing to obtain the rough product of rice bran polysaccharide; purifying the product through Q-Sepharose Big Beads anion exchange column chromatography and Sepharose CL-4B gel filter column chromatography grade by grade; obtaining the single polysaccharide with purity over 90% as raw material or additive of drug, chemical , hygienic and costemics.

Description

technical field [0001] A method for separating and purifying rice bran polysaccharides, the invention relates to the technical field of biological separation and purification, in particular to a process for separating and purifying rice bran polysaccharides. Background technique [0002] Rice bran polysaccharide exists in the caryopsis layer of rice, and it is one of the main nutritional components that has not been developed and widely used. Water-soluble rice bran polysaccharides are different from general homopolysaccharides. They are heteroglycans with complex structures, which are composed of xylose, mannose, rhamnose, galactose, arabinose and glucose. A variety of rice bran polysaccharides can be obtained by using different extraction processes of rice bran polysaccharides. They all have significant biological activity and health care functions. They not only have the physiological functions of general polysaccharides, but also have anti-tumor, blood sugar, cholesterol...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/10
Inventor 陈正行王莉张潇艳
Owner JIANGNAN UNIV
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