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Selection method for massively produced and used spirulina strains

A large-scale, spirulina technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high cost, long cycle, cumbersome procedures, etc., and achieve the effect of low cost

Inactive Publication Date: 2012-07-25
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

Although this method is practical and effective, the procedure is cumbersome, the workload is heavy, the cycle is long, and the cost is high.

Method used

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  • Selection method for massively produced and used spirulina strains
  • Selection method for massively produced and used spirulina strains
  • Selection method for massively produced and used spirulina strains

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Embodiment Construction

[0026] In our long-term molecular genetics research on Spirulina, we found that Spirulina, like other cyanobacteria such as Anabaena and Anabaena, has a special highly palindromic sequence ( highly iterated palindromic sequence, HIPS) [Microbiology, 1998, 144: 2791-2801]. The present invention establishes a new method of using the specific sequence HIPS-TG (5'-GCGATCGCTG-3') in HIPS as a primer combined with PCR (polymerase chain reaction) technology to determine whether the strain of spirulina can be applied to large-scale cultivation and production .

[0027] We used the specific sequence HIPS-TG (5'-GCGATCGCTG-3') in HIPS as primers to 14 strains of Spirulina ZJU0101, ZJU0102, ZJU0103, ZJU0104, ZJU0105, ZJU0106, ZJU0107, ZJU0108, ZJU0109 / RH, ZJU0110, ZJU01013, Z1U0101 , ZJU0115, and ZJU0116 were amplified by DNA polymorphism, and electrophoresis and cluster analysis found that the 14 strains could be divided into two categories: ZJU0103, ZJU0104, ZJU0105, ZJU0107, ZJU0108,...

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Abstract

The invention relates to the technology of spirulina strains development and application and aims to provide a selection method for massively produced and used spirulina strains. According to the method, a gene group deoxyribonucleic acid (DNA) is amplified through a specific primer high impact polystyrene total glycerin (HIPS-TG), and after electrophoretic analysis, NTSYSpc2.1 software is adopted to construct cluster diagram among the spirulina strains; if the candidate strains have special DNA electrophoretic bands with the length of 1,050bp and 760bp and can be clustered with the known strains with good production traits, the candidate strains are good in production traits and can be massively cultivated and produced; and if the candidate strains have the special DNA electrophoretic bands with the length of 1,520bp, 950bp, 720bp, 700bp and 500bp and can be clustered with the known strains with poor production traits, the candidate strains are poor in production traits and cannot be massively cultivated and produced. Compared with the conventional method, the method for identifying the spirulina strains with good production traits is simple, quick, efficient, low in cost and suitable for massive and high-throughput selection.

Description

technical field [0001] The invention belongs to the development and application technology of spirulina, in particular to a screening method suitable for large-scale production and application of spirulina strains. Background technique [0002] Spirulina is a kind of prokaryotic filamentous microalgae with photosynthetic oxygen release and regular spiral, which is a genus of Cyanophyta, Oscillatoriales and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4):369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge Spirulina industry on the basis of a large number of researches. It has become the world's largest research and development scale and the most extensive application prospects. microalgae. It is worth pointing out that numerous experimental studies and long-term production practices have shown that different varieties (lines) of Spirulina have di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 汪志平邵斌蓝瑾瑾刘新颖于金鑫王景梅陈子元
Owner ZHEJIANG UNIV
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