Protein extracting and separating method from defatted cotton dregs

A protein and absorbent cotton technology, applied in the field of protein extraction and separation, can solve the problems of difficulty in producing protein, huge production line equipment, deepening protein color, etc., and achieve the effects of reasonable equipment layout, low cost and less protein loss.

Inactive Publication Date: 2003-10-15
XINJIANG WESTERN ANIMAL HUSBANDRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the crushing and grinding process makes the equipment of the production line huge and the cost increases; secondly, the crushing and grinding can easily cause the pigment of the cotton husk in the cotton meal to enter the protein, causing the color of the protein to deepen, and the protein will also produce cotton husks. Impurities, these all affect the quality of protein, so the requirements for cotton meal are high, and the cotton shells in cotton meal are required to be less; third, because there is no homogenization and sterilization measures in the process, it is difficult to produce products that meet the national food hygiene standards. Fourth, in the process, the protein slurry is washed without neutralization after acid precipitation and centrifugation, which increases the loss of protein, and the acidity of the protein after drying is relatively strong, which also affects the quality of the protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 comprises the steps in the following order: degreased cotton meal is dissolved in alkali and centrifuged once to obtain solid phase and supernatant (I), the temperature during alkali dissolution is 50 ° C, and the pH value is 9, and the solid phase is dried to obtain feed Protein, supernatant (I) is subjected to acid precipitation, secondary separation, neutralization, homogeneous sterilization, and spray drying to obtain isolated protein. The pH value is 4.5 during acid precipitation, and the pH value is 7 during neutralization. The homogeneous sterilization is instantaneous sterilization , the temperature is 125°C.

Embodiment 2

[0017] Example 2, the difference between Example 2 and Example 1 is that the temperature during alkali dissolution is 35°C, the pH value is 12, the pH value during acid precipitation is 3.5, and the pH value during neutralization is 6.5, and the homogeneous sterilization is instantaneous sterilization. The temperature is 120°C.

Embodiment 3

[0018] Example 3, the difference between Example 3 and Example 1 is that the temperature during alkali dissolution is 65°C, the pH value is 8, the pH value during acid precipitation is 5.5, and the pH value during neutralization is 7.5, and the homogeneous sterilization is instantaneous sterilization. The temperature was 140°C.

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PUM

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Abstract

The process of extracting and separating protein from defatted cotton seed residue includes the following steps: alkali dissolving defatted cotton seed residue; first separation to obtain solid phaseand supernatant liquid; drying solid phase to obtain feed protein; and acid precipitation, second separation, neutralization, homogenization, sterilization and spray drying of the supernatant liquid to obtain protein. It has the advantages of reasonable apparatus layout, high quality of protein product, high protein extracting rate and no need of eliminating gossypol.

Description

technical field [0001] The invention relates to a method for extracting and separating protein from defatted cotton meal. Background technique [0002] Chinese patent CN1288676A discloses a "method for extracting protein from degreased cotton dregs", which includes the steps in the following order, first degreasing cotton dregs is pulverized and ground, water is added during grinding, and then filtrate and filter residue are obtained through leaching and separation. Collect the filtrate, keep the filtrate at a certain temperature, adjust the pH value to the isoelectric point to precipitate the protein, separate to obtain the supernatant and protein slurry (I), and wash and dry the protein slurry (I) to obtain the finished protein. The disadvantage is that the crushing and grinding process makes the equipment of the production line huge and the cost increases; secondly, the crushing and grinding can easily cause the pigment of the cotton husk in the cotton meal to enter the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23K10/30C07K1/14C07K2/00C07K14/415
Inventor 李树贤孙雪玲惠向斗
Owner XINJIANG WESTERN ANIMAL HUSBANDRY
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