33 results about "Formononetin" patented technology

The invention provides preparation of ROS responsive lipidosome and application of the ROS responsive lipidosome in anti-pancreatic cancer drugs, and belongs to the technical field of ROS responsive lipidosome preparation. The liposome can synergistically deliver formononetin and salvianolic acid B, overcomes the defects of formononetin and salvianolic acid B in the aspects of solubility, stability and bioavailability, and improves the curative effect of formononetin and salvianolic acid B in pancreatic cancer treatment. The ROS-responsive formononetin liposome and the salvianolic acid B liposome are wrapped by the cell-derived nano-vesicles through co-extrusion with the nano-vesicles on the outer layer, so that the ROS-responsive liposome is prepared. The liposome can be used for preparing anti-pancreatic cancer drugs, realizes targeted treatment of pancreatic cancer, inhibition of pancreatic cancer cell proliferation, promotion of pancreatic cancer cell apoptosis and improvement of pancreatic cancer tumor microenvironment energy metabolism disorder, and has the advantages of high biological safety, controllable drug release and the like.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

The invention discloses low-caffeine nerve stimulation tea wine and a preparation method thereof, the low-caffeine nerve stimulation tea wine is prepared by blending eucommia wood aged tea wine, cherry wood aged tea wine and cherry wood / Hainan dalbergia odorifera wood aged tea wine with different baking degrees, and the mass ratio of geniposidic acid to salidroside to formononetin in the wine body is (2-30): (1-15): 1. The mass ratio of the genkwanin to the alpinia japonica to the sesamol is (0.5-8): (0.15-6): 1, the mass ratio of the isoliquiritigenin to the vitexin to the syringaldehyde is 1: (0.15-5): (4-18), and the total content of the geniposidic acid, the salidroside, the formononetin, the genkwanin, the alpinia japonica, the sesamol, the isoliquiritigenin, the vitexin and the syringaldehyde in the wine is 40-250 mg / 100 mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The present application relates to the chemical medicine field, specifically, it relates to a kind of anti-tumor calophyllol derivative, preparation method and application, the calophyllol derivative has good inhibitory effect on thioredoxin reductase activity, and it is found that the molecule inhibits the activity of thioredoxin reductase by inhibiting the selenium cysteine of thioredoxin reductase carbon end, and then kills tumor cell, and then obtain the anti-tumor candidate drug with higher activity and better pharmacokinetic characteristics.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine nano preparation and application thereof in preparation of a medicine for preventing / treating PM2.5 induced lung injury. The invention provides a traditional Chinese medicine nano preparation. The traditional Chinese medicine nano preparation comprises nobiletin, formononetin and a carrier. According to the traditional Chinese medicine nano preparation, the nobiletin and the formononetin are entrapped in the carrier to form the nano particles with uniform particle size and good dispersity, so that the problems of poor water solubility and low bioavailability of the medicine can be effectively improved, the lung targeted delivery is enhanced, and the residence time of the medicine in the lung is prolonged. According to the traditional Chinese medicine nano preparation, the expression of ZO-1, OCLN, E-cad and other connexins is remarkably improved by inhibiting the EGFR pathway, the alveolar epithelial barrier structure and function are protected, and the inflammatory response and oxidative stress induced by PM2.5 are relieved, so that the lung injury induced by PM2.5 is effectively prevented and treated.

A small molecule composition, comprising licochalcone A, licochalcone C, licochalcone D, licochalcone E, echinatin, isoliquiritigenin, formononetin, liquiritigenin, licoflavone C, glabrol, licoisoflavone A and licoisoflavone B, and the use thereof in preparing anticancer or antitumor drugs, anti-ferroptosis drugs, or drugs for sensitization of anticancer or antitumor drugs. The small molecule composition can also be used in combination with other antitumor drugs.

The invention discloses a formononetin derivative containing piperidine-4-carbohydrazide as well as a preparation method and application of the formononetin derivative, and belongs to the technical field of pesticide synthesis. According to the invention, a piperidine-4-carbohydrazide group with excellent activity is introduced into the structure of formononetin, a series of formononetin derivatives of piperidine-4-carbohydrazide are synthesized, and the activity of the synthesized formononetin derivatives containing piperidine-4-carbohydrazide in inhibiting plant pathogens is tested. It is found that the formononetin derivative of piperidine-4-carbohydrazide synthesized by the invention can effectively inhibit plant pathogenic fungi, especially rhizoctonia solani.

The invention belongs to the field of cosmetics, and particularly relates to a composition for turning white hair into black hair as well as a preparation method, a product and application of the composition. The composition comprises the following effective components: a red formononetin root extract, a black oat seed extract, an emblic leafflower fruit extract and glycoprotein, the composition further comprises water, an ammonium acryloyldimethyl taurate / VP copolymer, butanediol, glycerin, trehalose, betaine, allantoin, phenoxyethanol and ethylhexylglycerin. The composition can directly stimulate the activity of melanin cells, promote synthesis and transportation of melanin and fundamentally promote blackening of white hair. The composition provided by the invention can directly solve the core mechanism of the white hair problem, and is expected to bring revolutionary breakthrough to treatment or prevention of white hair.

The invention relates to formononetin derivatives shown in a general formula III in the technical field of organic chemistry, and substituent groups R of the formononetin derivatives are defined in the specification. The formononetin derivative shown in the general formula III can be used for preparing antibacterial or bacteriostatic drugs, has remarkable antibacterial activity on staphylococcus aureus (S.aureus), escherichia coli (E.coli), methicillin-resistant staphylococcus aureus (MRSA) and the like, and is not resistant to drugs.

The invention provides application of a group of plasma metabolism markers in preparation of a reagent product for pulmonary nodule diagnosis, and relates to the technical field of disease diagnosis reagents. The metabolic marker is prepared from trigonelline, 4-methylcatechol, 2, 4-dinitrophenol, 3, 4-dihydroxyphenylacetic acid, N-(2-furfuryl) glycine, mevalonic acid and formononetin, and the metabolic marker is prepared from the following raw materials: the trigonelline, the 4-methylcatechol, the 2, 4-dinitrophenol, the 3, 4-dihydroxyphenylacetic acid, the N-( According to the application, ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC / MS) is adopted to carry out metabonomics analysis on plasma samples before and after an early pulmonary nodule operation, and a group of plasma metabolites (trigonelline, 4-methylcatechol, 2, 4-dinitrophenol, 3, 4-dihydroxyphenylacetic acid, N-(2-furfuryl) glycine, mevalonic acid and formononetin) are identified; and the group of metabolites is utilized to form a diagnosis model, so that benign and malignant pulmonary nodules can be accurately diagnosed, and early-stage differential diagnosis and risk assessment are facilitated.

The invention relates to a composite nano-system, a preparation method and application, the composite nano-system is a lipid-polymer hybrid nanoparticle with a core-shell structure, and the composite nano-system comprises: a hydrophobic core which is composed of a biodegradable polymer and cholesterol and is encapsulated with a hydrophobic drug formononetin; the middle layer is composed of cation / ionized lipid and auxiliary lipid and is used for loading negatively charged anti-KRAS siRNA through electrostatic interaction to form a stable compound, and the stable compound and the hydrophobic core jointly form nanoparticles; the shell layer is composed of pegylated lipid and a targeting ligand, and the targeting ligand is tLyp-1 peptide and is covalently linked to the tail end of the pegylated lipid through a maleimide-sulfydryl click chemical reaction. The system has excellent stability, biocompatibility and tumor targeting ability, can synergistically inhibit the growth of KRAS mutation pancreatic cancer, and relates to an application of the system in preparation of drugs for treating such cancers.

The invention belongs to the technical field of network pharmacology and molecular docking, and discloses a method for analyzing the treatment of renal fibrosis by formononetin based on the network pharmacology and molecular docking technology. Comprising the following steps: acquiring targets corresponding to active components, collecting renal fibrosis related targets, constructing a formononetin renal fibrosis target PPI network, performing enrichment analysis on core targets GO and KEGG, and performing molecular docking. Based on an integrated pharmacology platform and molecular docking, the mechanism of formononetin for treating renal fibrosis is explored, and the action mechanism of formononetin for treating renal fibrosis and related target genes are found and predicted.

The invention relates to application of formononetin in preparation of drugs, food or health care products for preventing or treating liver cancer related to non-alcoholic fatty liver disease or non-alcoholic fatty liver disease. According to the formononetin disclosed by the invention, the liver weight, the liver weight ratio, the tumor quantity and the maximum diameter of the tumor of a mouse with the non-alcoholic fatty liver-related liver cancer are remarkably reduced; in histology, the formononetin obviously improves fatty degeneration of liver tissues and liver lipid droplet density, and improves inflammation and fibrosis degree of liver cells; the formononetin obviously reduces the cholesterol serum content and the cholesterol content in the liver of a mouse with the non-alcoholic fatty liver related liver cancer, and reduces the liver injury indexes ALT and AST; the formononetin obviously reduces the expression level of mRNA (messenger ribonucleic acid) related to liver fibrosis of mice with liver cancer related to non-alcoholic fatty liver.

The present invention discloses a benzylpiperidine-containing formononetin derivative, a preparation method, and an application thereof, belonging to the technical field of pesticide synthesis. The present invention introduces a benzylpiperidine group with excellent activity into the structure of formononetin to synthesize a series of formononetin derivatives containing benzylpiperidine. Testing of the inhibitory activity of the synthesized formononetin derivatives containing benzylpiperidine against plant pathogenic bacteria reveals that the synthesized formononetin derivatives containing benzylpiperidine can effectively inhibit plant pathogenic bacteria, particularly Xanthomonas.

The invention pertains to the field of medical technology and health food, in particular, specifically providing a small molecule composition that exerts negative regulation on the Nrf2 signaling pathway. This composition comprises licorice chalcone A, licorice chalcone C, licorice chalcone D, licorice chalcone E, formononetin, glabrone and Licoflavone C, which can be utilized for the prevention or treatment of non-alcoholic fatty liver disease.

The invention discloses a formononetin derivative containing indole as well as a preparation method and application of the formononetin derivative, and belongs to the technical field of pesticide synthesis. According to the invention, an indole group with good biological activity is introduced into a formononetin structure, a series of formononetin derivatives containing indole are designed and synthesized, and the synthesized formononetin derivatives containing indole are subjected to plant pathogen inhibition activity tests. It is found that the derivative can effectively inhibit various plant pathogenic fungi, and especially shows the strongest inhibitory activity on Phomopsis kiwifruit.

The application belongs to the field of medicine and relates to application of an isoflavone compound, isoformononetin (IFM), in preparation of a medicine or health care product for preventing and treating depression; in mouse hippocampal neuron cells (HT22) or mouse microglial cells (BV2), IFM can reduce neuroinflammation caused by corticosterone (CORT) or lipopolysaccharide (LPS) and increase the expression level of brain-derived neurotrophic factor (BDNF); in a chronic mild unpredictable stress mouse depression model, IFM can relieve depression-like behavior of the mouse and reduce the inflammation level of brain tissue, indicating that IFM has a good effect of relieving depression; IFM degrades PDE4D protein by directly combining with a PDE4D target protein. The isoflavone compound IFM has the advantages of outstanding effect and high safety and is expected to develop into an effective medicine for treating and preventing depression.

This invention relates to the field of biotechnology, specifically to the construction and application of a Benzodiacetic tobacco chassis for synthesizing isoflavones. This invention reconstructs the upstream pathway for isoflavone synthesis in Benzodiacetic tobacco, further identifies and screens key rate-limiting enzymes for isoflavone synthesis in Benzodiacetic tobacco, and utilizes 2A peptides to co-express multiple key genes, further introducing flavonoid synthesis transcription factors to construct a Benzodiacetic tobacco chassis for synthesizing isoflavones. This Benzodiacetic tobacco chassis can efficiently synthesize daidzein and genistein. Furthermore, based on the constructed high-yield Benzodiacetic tobacco platform for daidzein and genistein, various isoflavones can be further synthesized, such as daidzein, genistein, puerarin, gentiopicrin, chickpea extract A, and stigmosiderin, paving the way for the industrialization of plant biosynthesis of various bioactive isoflavones and thus possessing broad application prospects.

The invention discloses a traditional Chinese medicine composition and application, and relates to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises, by weight, 1 part of sinomenine and 0.5-1.5 parts of formononetin, and the traditional Chinese medicine composition is used for treating chicken mycoplasma synoviae. As an innovative therapy for treating chicken mycoplasma synoviae, the traditional Chinese medicine composition provides an efficient and resistance-free treatment choice for farmers due to the characteristics of simple formula and easiness in obtaining. The traditional Chinese medicine composition is only composed of two traditional Chinese medicines, the cost is reduced, the preparation process is simplified, and large-scale application becomes possible. More importantly, the composition avoids negative effects caused by use of antibiotics and is especially suitable for the situation that antibiotics cannot be abused in laying hen breeding, a breeder can effectively control diseases and reduce economic losses under the situation that food safety regulations are not violated, the safety of poultry products is guaranteed, and the requirements of consumers for antibiotic-free products are met.

This invention belongs to the field of traditional Chinese medicine, specifically relating to a purified flavonoid from Sophora flavescens, its preparation method, and its application. Using Sophora flavescens as raw material, this invention extracts and separates purified flavonoid KSF50, and uses liquid chromatography-mass spectrometry (LC-MS / MS) to analyze and identify its components, determining its main functional groups and composition. The purified flavonoid from Sophora flavescens mainly contains the following six flavonoid components: daidzein, gentiopicrin, matrine O, trifolin stigmosiderin, matrine, and sophoridine. Furthermore, based on a high-sugar diet-induced *C. elegans* model and a high-sugar-induced 3T3-L1 preadipocyte model, this invention provides evidence that Sophora flavescens flavonoids can delay the aging of *C. elegans* by regulating lipid metabolism disorders, providing a theoretical basis for its application in medicine, cosmetics, and health functional foods.

The invention discloses beta-1, 6-glucosidic bond hydrolase and application thereof in traditional Chinese medicine resources. According to the method, a key gene sequence with a beta-1, 6-glucosidic bond hydrolysis function is screened from a Candida pseudoglabrata L7 genome and is named as CoExg1. A gene engineering bacterium capable of efficiently and heterologously expressing CoExg1 is constructed by utilizing a molecular cloning technology. According to the method, siamenoside I and mogroside IIIE are used as raw materials, an enzyme method catalytic reaction system is established after enzyme production through fermentation, traditional Chinese medicine active molecules of mogroside V are directionally converted, beta-1, 6-glucosidic bonds in different areas in a substrate structure are selectively hydrolyzed, and therefore efficient staged synthesis of different products such as siamenoside I and mogroside IIIE is achieved. Besides, the CoExg1 can hydrolyze beta-glucosidic bonds in isopentenyl flavone icariin and formononetin structures, icariside II and formononetin are respectively and directionally synthesized, and an important technical support is provided for high-value utilization of traditional Chinese medicine resources and quality and efficiency improvement of traditional Chinese medicine glycoside.

The invention discloses a method for predicting a liver cancer immune drug resistance scheme of formononetin targeting ERalpha36. The method comprises the following steps: acquiring current multi-modal pathological data of a liver cancer patient; inputting the current multi-modal pathological data into a multi-modal graph neural network to predict a treatment scheme to obtain formononetin treatment schemes of the liver cancer patient under different drug resistance probabilities; the multi-modal graph neural network is constructed by training a graph attention network carrying ERalpha36 bias enhancement based on a historical experiment data set; the historical experiment data set comprises historical multi-mode pathological data with a corresponding relation and formononetin treatment schemes under various historical drug resistance probabilities; according to the Shapley value, quantifying an accumulated contribution value corresponding to the formononetin treatment scheme under each drug resistance probability; and determining the formononetin treatment scheme under the drug resistance probability corresponding to the highest accumulated contribution value as the optimal treatment scheme for the liver cancer patient. The application can improve the effectiveness and safety of immune drug-resistant treatment of liver cancer.

The invention relates to a B cell inhibitor and application thereof in preparation of drugs for treating xerophthalmia. The present invention relates to a B cell activity inhibitor selected from the group consisting of formononetin; formononin; astragaloside IV; total flavonoids of an astragalus extract; the components are astragaloside IV and formononetin; astragaloside IV and formononin; the composition is prepared from astragaloside I, astragaloside IV, formononin and formononetin. Compared with the side effect of cyclosporine in the prior art (which is within the renal toxicity of 1 / 3 of a patient who takes the medicine), the astragalus extract general flavone has the effect of inhibiting the activity of B cells so as to inhibit humoral immunity. On the basis of the research on the disease mechanism of pSS, the specific astragalus membranaceus extract general flavone which can be used as a medicine for administration and a medicine for instillation is developed.

The invention provides an application of a methoxy isoflavone compound in prevention and treatment of premature ovarian failure. A large number of studies find that the biochanin A and formononetin can effectively improve ovarian reserve function decline or estrus cycle disorder caused by premature senility, adjust the estrogen level, promote follicle development and maturity, increase the ovarian index, increase ovarian reserve and recover the ovarian function. Meanwhile, the biochanin A and the formononetin have the advantages of good stability, high biological activity and low toxicity, can be safely used for preparing medicines for preventing or / and treating ovarian reserve hypofunction and premature senility, and have good patent medicine prospects.

The present application relates to the chemical medicine field, specifically, a kind of formononetin derivative, preparation method and its application, the compound has significant proliferation inhibitory effect to EGFR high expression tumor cell, and activity is significantly better than formononetin;To EGFR low expression tumor cell and normal cell, toxicity is lower, has good target selectivity and biocompatibility;To A431 and HCC827 cell EGFR phosphorylation has good inhibitory effect, and it is dose-dependent;The compound mainly kills tumor cell by inducing apoptosis, with the gradual increase of compound concentration, early apoptotic cell and late apoptotic cell number also gradually increase, it is explained that compound mainly kills tumor cell by inducing apoptosis, provides potential candidate for developing new EGFR targeted antitumor drug, has important research value and application prospect.

The invention discloses formononetin derivatives and application thereof in preparation of medicines for treating central nervous system diseases, and belongs to the technical field of biological medicines. The formononetin derivative has a good effect of reducing the NO release level of BV-2 inflammatory cells, is dose-dependent, successfully enhances the anti-inflammatory activity of formononetin, almost has no cytotoxicity to BV-2 cells, and is good in safety; compared with traditional anti-inflammatory drugs, the compound has the advantages of remarkable anti-inflammatory activity and low cytotoxicity, and is expected to become a new generation of anti-central nervous inflammation drugs; on the basis of a formononetin molecular structure, the formononetin is obtained through SN2 substitution reaction, the operation is simple, the reaction conditions are controllable, and a series of derivatives can be efficiently synthesized; when being applied to preparation of the anti-central nervous system medicine, the compound has a good effect of reducing NO content of BV-2 inflammatory cells, is dose-dependent, also has a concentration-dependent inhibition effect on secretion of inflammatory factors such as IL-6 and TNF-alpha, regulates inflammatory reaction from a plurality of key links, and has a positive effect on related research of later brain diseases. The compound has a good effect of reducing NO content of BV-2 inflammatory cells, is dose-dependent and has a concentration-dependent inhibition effect on secretion of inflammatory factors such as IL-6 and TNF-alpha.

The invention relates to the technical field of hedysarum polybotrys processing, in particular to a processing method for improving the quality of hedysarum polybotrys by adopting kneading sweating, which comprises the following steps: S1, digging and pretreatment; s2, performing airing treatment; s3, carrying out refined kneading treatment; s4, performing sweating treatment by stages; s5, drying and shaping treatment; through diameter classification and multi-step refined kneading, the problems of poor skin and flesh fitting and strip shape bending during processing can be avoided, the final finished product radix hedysari is oily and compact in appearance, the quality of the radix hedysari is improved, the content of formononetin in the finished product radix hedysari can be improved by means of a core technology of staged sweating, the content of effective components is stable, and the medicinal value is improved. By means of ozone disinfection before sweating, cushion layer high-temperature sterilization, mildew early warning treatment and accurate moisture detection, the problem of loss caused by mildew and excessive moisture during processing can be prevented, and the quality of the hedysarum polybotrys is further improved.

The application discloses application of berberine combined with formononetin in preparation of an antitumor drug, and particularly relates to treatment of nasopharyngeal carcinoma. After the two drugs are combined, an unexpected synergistic effect exists, and no toxic side effect is caused, so that the application has great application value in treatment of nasopharyngeal carcinoma.

The invention provides astragalus mongholicus fermentation liquor and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine ferment.The preparation method of the astragalus mongholicus fermentation liquor comprises the steps that a culture medium containing astragalus mongholicus alcohol extract is inoculated with lactobacillus reuteri for fermentation and concentration, and the astragalus mongholicus fermentation liquor is obtained; wherein the main component of the radix astragali alcohol extract is flavonoids which do not contain polysaccharide; the content of formononetin in the astragalus mongholicus fermentation liquor is more than two times that of the astragalus mongholicus alcohol extract; the astragalus mongholicus fermentation liquor obtained by the method disclosed by the invention can be used for remarkably relieving the overall symptoms of ulcerative colitis model mice, including inhibiting weight loss, diarrhea and hemafecia symptoms, improving colonic mucosa inflammatory cell infiltration, goblet cell disappearance and crypt structure damage, reducing the content of colonic tissue inflammatory cytokines, and improving the ulcerative colitis model mice. And ulcerative colitis mouse crus gastrocnemius atrophy can be relieved.

The invention relates to the technical field of quality standards and detection of traditional Chinese medicine formulae, and discloses a method for constructing a fingerprint spectrum of a water-soluble extract of a caulis spatholobi traditional Chinese medicine compound, the fingerprint spectrum is established through liquid chromatography, and germacrone, epicatechin, formononetin, daidzein, polyphyllin VII and saikoside d are used as reference substances; and separating the to-be-detected sample by adopting a gradient elution mode to obtain the fingerprint spectrum of the water-soluble extract of the caulis spatholobi traditional Chinese medicine compound. The fingerprint detection method for the water-soluble extract of the caulis spatholobi traditional Chinese medicine compound has the advantages of good stability, high precision, good repeatability and the like, and can be used for accurately analyzing effective components in the water-soluble extract of the caulis spatholobi traditional Chinese medicine compound; and a new method is provided for comprehensively and objectively evaluating the quality of the water-soluble extract of the caulis spatholobi traditional Chinese medicine compound.