43 results about "Formononetin" patented technology

The invention provides preparation of ROS responsive lipidosome and application of the ROS responsive lipidosome in anti-pancreatic cancer drugs, and belongs to the technical field of ROS responsive lipidosome preparation. The liposome can synergistically deliver formononetin and salvianolic acid B, overcomes the defects of formononetin and salvianolic acid B in the aspects of solubility, stability and bioavailability, and improves the curative effect of formononetin and salvianolic acid B in pancreatic cancer treatment. The ROS-responsive formononetin liposome and the salvianolic acid B liposome are wrapped by the cell-derived nano-vesicles through co-extrusion with the nano-vesicles on the outer layer, so that the ROS-responsive liposome is prepared. The liposome can be used for preparing anti-pancreatic cancer drugs, realizes targeted treatment of pancreatic cancer, inhibition of pancreatic cancer cell proliferation, promotion of pancreatic cancer cell apoptosis and improvement of pancreatic cancer tumor microenvironment energy metabolism disorder, and has the advantages of high biological safety, controllable drug release and the like.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

The invention discloses low-caffeine nerve stimulation tea wine and a preparation method thereof, the low-caffeine nerve stimulation tea wine is prepared by blending eucommia wood aged tea wine, cherry wood aged tea wine and cherry wood / Hainan dalbergia odorifera wood aged tea wine with different baking degrees, and the mass ratio of geniposidic acid to salidroside to formononetin in the wine body is (2-30): (1-15): 1. The mass ratio of the genkwanin to the alpinia japonica to the sesamol is (0.5-8): (0.15-6): 1, the mass ratio of the isoliquiritigenin to the vitexin to the syringaldehyde is 1: (0.15-5): (4-18), and the total content of the geniposidic acid, the salidroside, the formononetin, the genkwanin, the alpinia japonica, the sesamol, the isoliquiritigenin, the vitexin and the syringaldehyde in the wine is 40-250 mg / 100 mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The present application relates to the chemical medicine field, specifically, it relates to a kind of anti-tumor calophyllol derivative, preparation method and application, the calophyllol derivative has good inhibitory effect on thioredoxin reductase activity, and it is found that the molecule inhibits the activity of thioredoxin reductase by inhibiting the selenium cysteine of thioredoxin reductase carbon end, and then kills tumor cell, and then obtain the anti-tumor candidate drug with higher activity and better pharmacokinetic characteristics.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine nano preparation and application thereof in preparation of a medicine for preventing / treating PM2.5 induced lung injury. The invention provides a traditional Chinese medicine nano preparation. The traditional Chinese medicine nano preparation comprises nobiletin, formononetin and a carrier. According to the traditional Chinese medicine nano preparation, the nobiletin and the formononetin are entrapped in the carrier to form the nano particles with uniform particle size and good dispersity, so that the problems of poor water solubility and low bioavailability of the medicine can be effectively improved, the lung targeted delivery is enhanced, and the residence time of the medicine in the lung is prolonged. According to the traditional Chinese medicine nano preparation, the expression of ZO-1, OCLN, E-cad and other connexins is remarkably improved by inhibiting the EGFR pathway, the alveolar epithelial barrier structure and function are protected, and the inflammatory response and oxidative stress induced by PM2.5 are relieved, so that the lung injury induced by PM2.5 is effectively prevented and treated.

A small molecule composition, comprising licochalcone A, licochalcone C, licochalcone D, licochalcone E, echinatin, isoliquiritigenin, formononetin, liquiritigenin, licoflavone C, glabrol, licoisoflavone A and licoisoflavone B, and the use thereof in preparing anticancer or antitumor drugs, anti-ferroptosis drugs, or drugs for sensitization of anticancer or antitumor drugs. The small molecule composition can also be used in combination with other antitumor drugs.

The invention discloses a formononetin derivative containing piperidine-4-carbohydrazide as well as a preparation method and application of the formononetin derivative, and belongs to the technical field of pesticide synthesis. According to the invention, a piperidine-4-carbohydrazide group with excellent activity is introduced into the structure of formononetin, a series of formononetin derivatives of piperidine-4-carbohydrazide are synthesized, and the activity of the synthesized formononetin derivatives containing piperidine-4-carbohydrazide in inhibiting plant pathogens is tested. It is found that the formononetin derivative of piperidine-4-carbohydrazide synthesized by the invention can effectively inhibit plant pathogenic fungi, especially rhizoctonia solani.

The porphyrin is bonded with the natural product isoflavone formononetin with antitumor activity, so that on one hand, the PDT effect of the porphyrin can be played to inhibit the growth and reproduction of tumor cells, and on the other hand, the tumor affinity characteristic of the porphyrin can be played, the formononetin with antitumor activity is positioned to a tumor part, the damage to normal tissues is reduced, and the antitumor activity of the formononetin is improved. The cytotoxicity is reduced.

The invention belongs to the field of cosmetics, and particularly relates to a composition for turning white hair into black hair as well as a preparation method, a product and application of the composition. The composition comprises the following effective components: a red formononetin root extract, a black oat seed extract, an emblic leafflower fruit extract and glycoprotein, the composition further comprises water, an ammonium acryloyldimethyl taurate / VP copolymer, butanediol, glycerin, trehalose, betaine, allantoin, phenoxyethanol and ethylhexylglycerin. The composition can directly stimulate the activity of melanin cells, promote synthesis and transportation of melanin and fundamentally promote blackening of white hair. The composition provided by the invention can directly solve the core mechanism of the white hair problem, and is expected to bring revolutionary breakthrough to treatment or prevention of white hair.

The invention relates to formononetin derivatives shown in a general formula III in the technical field of organic chemistry, and substituent groups R of the formononetin derivatives are defined in the specification. The formononetin derivative shown in the general formula III can be used for preparing antibacterial or bacteriostatic drugs, has remarkable antibacterial activity on staphylococcus aureus (S.aureus), escherichia coli (E.coli), methicillin-resistant staphylococcus aureus (MRSA) and the like, and is not resistant to drugs.

The invention provides application of a group of plasma metabolism markers in preparation of a reagent product for pulmonary nodule diagnosis, and relates to the technical field of disease diagnosis reagents. The metabolic marker is prepared from trigonelline, 4-methylcatechol, 2, 4-dinitrophenol, 3, 4-dihydroxyphenylacetic acid, N-(2-furfuryl) glycine, mevalonic acid and formononetin, and the metabolic marker is prepared from the following raw materials: the trigonelline, the 4-methylcatechol, the 2, 4-dinitrophenol, the 3, 4-dihydroxyphenylacetic acid, the N-( According to the application, ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC / MS) is adopted to carry out metabonomics analysis on plasma samples before and after an early pulmonary nodule operation, and a group of plasma metabolites (trigonelline, 4-methylcatechol, 2, 4-dinitrophenol, 3, 4-dihydroxyphenylacetic acid, N-(2-furfuryl) glycine, mevalonic acid and formononetin) are identified; and the group of metabolites is utilized to form a diagnosis model, so that benign and malignant pulmonary nodules can be accurately diagnosed, and early-stage differential diagnosis and risk assessment are facilitated.

The invention relates to a composite nano-system, a preparation method and application, the composite nano-system is a lipid-polymer hybrid nanoparticle with a core-shell structure, and the composite nano-system comprises: a hydrophobic core which is composed of a biodegradable polymer and cholesterol and is encapsulated with a hydrophobic drug formononetin; the middle layer is composed of cation / ionized lipid and auxiliary lipid and is used for loading negatively charged anti-KRAS siRNA through electrostatic interaction to form a stable compound, and the stable compound and the hydrophobic core jointly form nanoparticles; the shell layer is composed of pegylated lipid and a targeting ligand, and the targeting ligand is tLyp-1 peptide and is covalently linked to the tail end of the pegylated lipid through a maleimide-sulfydryl click chemical reaction. The system has excellent stability, biocompatibility and tumor targeting ability, can synergistically inhibit the growth of KRAS mutation pancreatic cancer, and relates to an application of the system in preparation of drugs for treating such cancers.

The invention provides a feed additive for improving the superovulation effect of cows and a preparation method of the feed additive, and the feed additive comprises the following components in parts by mass: 4-6 parts of astragaloside, 4-6 parts of apigenin, 4-6 parts of kaempferol, 0.4-0.6 part of luteolin, 0.4-0.6 part of isoquercitrin, 0.2-0.4 part of formononetin and 0.2-0.4 part of quercetin. By using the feed additive, the ovulation number and the anti-oxidation and anti-inflammatory capacity of cows can be increased, meanwhile, the natural active ingredients in the feed additive do not need complex chemical synthesis steps, the breeding cost is reduced, animal health is improved, green and safe breeding is guaranteed, and a basis is provided for green development of modern animal husbandry.

The invention belongs to the technical field of network pharmacology and molecular docking, and discloses a method for analyzing the treatment of renal fibrosis by formononetin based on the network pharmacology and molecular docking technology. Comprising the following steps: acquiring targets corresponding to active components, collecting renal fibrosis related targets, constructing a formononetin renal fibrosis target PPI network, performing enrichment analysis on core targets GO and KEGG, and performing molecular docking. Based on an integrated pharmacology platform and molecular docking, the mechanism of formononetin for treating renal fibrosis is explored, and the action mechanism of formononetin for treating renal fibrosis and related target genes are found and predicted.

The invention relates to application of formononetin in preparation of drugs, food or health care products for preventing or treating liver cancer related to non-alcoholic fatty liver disease or non-alcoholic fatty liver disease. According to the formononetin disclosed by the invention, the liver weight, the liver weight ratio, the tumor quantity and the maximum diameter of the tumor of a mouse with the non-alcoholic fatty liver-related liver cancer are remarkably reduced; in histology, the formononetin obviously improves fatty degeneration of liver tissues and liver lipid droplet density, and improves inflammation and fibrosis degree of liver cells; the formononetin obviously reduces the cholesterol serum content and the cholesterol content in the liver of a mouse with the non-alcoholic fatty liver related liver cancer, and reduces the liver injury indexes ALT and AST; the formononetin obviously reduces the expression level of mRNA (messenger ribonucleic acid) related to liver fibrosis of mice with liver cancer related to non-alcoholic fatty liver.

The present invention discloses a benzylpiperidine-containing formononetin derivative, a preparation method, and an application thereof, belonging to the technical field of pesticide synthesis. The present invention introduces a benzylpiperidine group with excellent activity into the structure of formononetin to synthesize a series of formononetin derivatives containing benzylpiperidine. Testing of the inhibitory activity of the synthesized formononetin derivatives containing benzylpiperidine against plant pathogenic bacteria reveals that the synthesized formononetin derivatives containing benzylpiperidine can effectively inhibit plant pathogenic bacteria, particularly Xanthomonas.

The invention discloses a formononetin derivative containing a piperidine skeleton as well as a preparation method and application of the formononetin derivative, and belongs to the technical field of pesticide synthesis. According to the invention, formononetin is taken as a leading structure, a piperidine skeleton with excellent agricultural activity is introduced into the structure of formononetin, a series of formononetin derivatives containing the piperidine skeleton are synthesized, and the activity of the synthesized formononetin derivatives containing the piperidine skeleton in inhibiting plant pathogenic bacteria is tested, so that the activity of the formononetin derivatives containing the piperidine skeleton in inhibiting plant pathogenic bacteria is obviously improved. It is found that the formononetin derivative containing the piperidine skeleton synthesized by the invention can effectively inhibit plant pathogenic bacteria.

The invention pertains to the field of medical technology and health food, in particular, specifically providing a small molecule composition that exerts negative regulation on the Nrf2 signaling pathway. This composition comprises licorice chalcone A, licorice chalcone C, licorice chalcone D, licorice chalcone E, formononetin, glabrone and Licoflavone C, which can be utilized for the prevention or treatment of non-alcoholic fatty liver disease.

The invention discloses a formononetin derivative containing indole as well as a preparation method and application of the formononetin derivative, and belongs to the technical field of pesticide synthesis. According to the invention, an indole group with good biological activity is introduced into a formononetin structure, a series of formononetin derivatives containing indole are designed and synthesized, and the synthesized formononetin derivatives containing indole are subjected to plant pathogen inhibition activity tests. It is found that the derivative can effectively inhibit various plant pathogenic fungi, and especially shows the strongest inhibitory activity on Phomopsis kiwifruit.

The application belongs to the field of medicine and relates to application of an isoflavone compound, isoformononetin (IFM), in preparation of a medicine or health care product for preventing and treating depression; in mouse hippocampal neuron cells (HT22) or mouse microglial cells (BV2), IFM can reduce neuroinflammation caused by corticosterone (CORT) or lipopolysaccharide (LPS) and increase the expression level of brain-derived neurotrophic factor (BDNF); in a chronic mild unpredictable stress mouse depression model, IFM can relieve depression-like behavior of the mouse and reduce the inflammation level of brain tissue, indicating that IFM has a good effect of relieving depression; IFM degrades PDE4D protein by directly combining with a PDE4D target protein. The isoflavone compound IFM has the advantages of outstanding effect and high safety and is expected to develop into an effective medicine for treating and preventing depression.

This invention relates to the field of biotechnology, specifically to the construction and application of a Benzodiacetic tobacco chassis for synthesizing isoflavones. This invention reconstructs the upstream pathway for isoflavone synthesis in Benzodiacetic tobacco, further identifies and screens key rate-limiting enzymes for isoflavone synthesis in Benzodiacetic tobacco, and utilizes 2A peptides to co-express multiple key genes, further introducing flavonoid synthesis transcription factors to construct a Benzodiacetic tobacco chassis for synthesizing isoflavones. This Benzodiacetic tobacco chassis can efficiently synthesize daidzein and genistein. Furthermore, based on the constructed high-yield Benzodiacetic tobacco platform for daidzein and genistein, various isoflavones can be further synthesized, such as daidzein, genistein, puerarin, gentiopicrin, chickpea extract A, and stigmosiderin, paving the way for the industrialization of plant biosynthesis of various bioactive isoflavones and thus possessing broad application prospects.

The present invention relates to an acne-removing gel preparation, wherein the weight ratio of the active ingredients lysozyme, puerarin and formononetin is 1:(1-5):(1-5). The preparation method of the acne-removing gel preparation of the present invention is simple and the quality is stable. While the three active ingredients play their respective functions, they can also synergistically treat acne caused by bacteria such as Propionibacterium acnes. In addition, lysozyme, puerarin and formononetin are naturally sourced and have no toxic side effects.

The invention discloses a traditional Chinese medicine composition and application, and relates to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises, by weight, 1 part of sinomenine and 0.5-1.5 parts of formononetin, and the traditional Chinese medicine composition is used for treating chicken mycoplasma synoviae. As an innovative therapy for treating chicken mycoplasma synoviae, the traditional Chinese medicine composition provides an efficient and resistance-free treatment choice for farmers due to the characteristics of simple formula and easiness in obtaining. The traditional Chinese medicine composition is only composed of two traditional Chinese medicines, the cost is reduced, the preparation process is simplified, and large-scale application becomes possible. More importantly, the composition avoids negative effects caused by use of antibiotics and is especially suitable for the situation that antibiotics cannot be abused in laying hen breeding, a breeder can effectively control diseases and reduce economic losses under the situation that food safety regulations are not violated, the safety of poultry products is guaranteed, and the requirements of consumers for antibiotic-free products are met.

This invention belongs to the field of traditional Chinese medicine, specifically relating to a purified flavonoid from Sophora flavescens, its preparation method, and its application. Using Sophora flavescens as raw material, this invention extracts and separates purified flavonoid KSF50, and uses liquid chromatography-mass spectrometry (LC-MS / MS) to analyze and identify its components, determining its main functional groups and composition. The purified flavonoid from Sophora flavescens mainly contains the following six flavonoid components: daidzein, gentiopicrin, matrine O, trifolin stigmosiderin, matrine, and sophoridine. Furthermore, based on a high-sugar diet-induced *C. elegans* model and a high-sugar-induced 3T3-L1 preadipocyte model, this invention provides evidence that Sophora flavescens flavonoids can delay the aging of *C. elegans* by regulating lipid metabolism disorders, providing a theoretical basis for its application in medicine, cosmetics, and health functional foods.

The invention provides a composition. The composition comprises genistein and formononetin, the content ratio of the genistein to the formononetin is (1): (3). According to the composition with the specific proportion, the hair fixing effect after hair transplantation is achieved, and after hair transplantation, hair can be subjected to a first-stage wound healing, a second-stage hair loss, a resting stage and a third-stage regrowth stage. In the process, formononetin mainly plays an anti-inflammatory role in the first stage, and promotes generation of Laminin in the second stage, so that the effects of stabilizing hair follicles and the like are achieved; genistein focuses on promoting hair to be converted from a resting stage to a growing stage by adjusting a GSK-3beta-beta-catenin signal channel in the second stage and inhibiting an AR receptor in the third stage so as to reduce sebum secretion. Due to different functions of the two components, the two components are combined together, so that a patient after hair transplantation can be more effectively nursed, and the hair fixing effect after hair transplantation is realized.

The invention discloses beta-1, 6-glucosidic bond hydrolase and application thereof in traditional Chinese medicine resources. According to the method, a key gene sequence with a beta-1, 6-glucosidic bond hydrolysis function is screened from a Candida pseudoglabrata L7 genome and is named as CoExg1. A gene engineering bacterium capable of efficiently and heterologously expressing CoExg1 is constructed by utilizing a molecular cloning technology. According to the method, siamenoside I and mogroside IIIE are used as raw materials, an enzyme method catalytic reaction system is established after enzyme production through fermentation, traditional Chinese medicine active molecules of mogroside V are directionally converted, beta-1, 6-glucosidic bonds in different areas in a substrate structure are selectively hydrolyzed, and therefore efficient staged synthesis of different products such as siamenoside I and mogroside IIIE is achieved. Besides, the CoExg1 can hydrolyze beta-glucosidic bonds in isopentenyl flavone icariin and formononetin structures, icariside II and formononetin are respectively and directionally synthesized, and an important technical support is provided for high-value utilization of traditional Chinese medicine resources and quality and efficiency improvement of traditional Chinese medicine glycoside.

The invention discloses a method for predicting a liver cancer immune drug resistance scheme of formononetin targeting ERalpha36. The method comprises the following steps: acquiring current multi-modal pathological data of a liver cancer patient; inputting the current multi-modal pathological data into a multi-modal graph neural network to predict a treatment scheme to obtain formononetin treatment schemes of the liver cancer patient under different drug resistance probabilities; the multi-modal graph neural network is constructed by training a graph attention network carrying ERalpha36 bias enhancement based on a historical experiment data set; the historical experiment data set comprises historical multi-mode pathological data with a corresponding relation and formononetin treatment schemes under various historical drug resistance probabilities; according to the Shapley value, quantifying an accumulated contribution value corresponding to the formononetin treatment scheme under each drug resistance probability; and determining the formononetin treatment scheme under the drug resistance probability corresponding to the highest accumulated contribution value as the optimal treatment scheme for the liver cancer patient. The application can improve the effectiveness and safety of immune drug-resistant treatment of liver cancer.

The invention relates to a B cell inhibitor and application thereof in preparation of drugs for treating xerophthalmia. The present invention relates to a B cell activity inhibitor selected from the group consisting of formononetin; formononin; astragaloside IV; total flavonoids of an astragalus extract; the components are astragaloside IV and formononetin; astragaloside IV and formononin; the composition is prepared from astragaloside I, astragaloside IV, formononin and formononetin. Compared with the side effect of cyclosporine in the prior art (which is within the renal toxicity of 1 / 3 of a patient who takes the medicine), the astragalus extract general flavone has the effect of inhibiting the activity of B cells so as to inhibit humoral immunity. On the basis of the research on the disease mechanism of pSS, the specific astragalus membranaceus extract general flavone which can be used as a medicine for administration and a medicine for instillation is developed.

The present invention relates to active ingredient separation and extraction technology, and specifically to a highly efficient preparation method and application of flavonoids from peanut stems and leaves. A crude peanut stem and leaf extract is subjected to gradient elution separation by high-speed countercurrent chromatography using a stationary phase, sequentially employing a first mobile phase, a second mobile phase, and a third mobile phase, to obtain daidzein and formononetin. The stationary phase comprises an upper phase of n-hexane, ethyl acetate, methanol, and water (5:4.8-5.2:1.9-2.1:7.6-8.4), the first mobile phase comprises a lower phase of n-hexane, ethyl acetate, methanol, and water (5:4.8-5.2:2.9-3.1:6.6-7.4), and the third mobile phase comprises a lower phase of n-hexane, ethyl acetate, methanol, and water (5:4.8-5.2:4.8-5.2:4.8-5.2). The present invention enables high-speed and efficient extraction of daidzein and formononetin.

The invention discloses an application of a formononetin derivative containing benzyl piperidine in preparation of a medicine for inhibiting breast cancer MDA-MB-231 cells. All target compounds have certain in-vitro inhibitory activity on MDA-MB-231 at the concentration of 1 mu M and 10 mu M. Wherein under the concentration of 10 mu M, except H22, the inhibitory activity of other compounds is far higher than that of gefitinib.