196 results about "Lycoris radiata" patented technology

Disclosed is a high-efficiency lycoris and citrus interplanting method. The high-efficiency lycoris and citrus interplanting method includes interplanting lycoris under fruit trees such as citrus, covering the lycoris by rotten animal manure straws, managing by processes of fertilizing, weeding, insecticiding and the like, and harvesting after five-year planting. By the high-efficiency lycoris and citrus interplanting method, two kinds of economic plants can be produced on the same field, multiple cropping index is increased, labor cost is saved, production cost is reduced, yield is increased, and economic benefits are doubly increased.

The invention relates to a method for producing the lycoris by plant tissue culture, which comprises the steps of: (1) taking an MS (mass spectrometry) culture medium as a basic culture medium, and taking a 2, 4-D, IAA (indo acetic acid), NAA (naphthyl acetic acid), 6-BA (butyl acrylate) and IBA (iso butyl alcohol) as a regulated and controlled growth hormone preparation culture medium; (2) taking the bulb of the lycoris as an explant, and sterilizing; (3) vaccinating the explant of the bulb on a bulb inducing culture medium to culture in an inducing way so as to grow bulbils; (4) transferring the bulbils into a rooting culture medium to culture in a rooting way, so that the bulbils can independently absorb the nutrient elements from the soil and the matrix; (5) transferring the bulbils into the culture medium to culture; (6) opening an opening of a vaccination bottle to refine the seedling; withdrawing the bulbils, and transferring the bulbils into a nutrient cup which takes the vermiculite, the pearlite and the nutrient soil as the mixed matrix; and transferring the bulbils into a large tent, so that the survival rate reaches 94%-96%. The method is less in material taking, and economical in culturing; the culture condition can be manually controlled and is not influenced by the natural condition; the method is short in growing period, and high in propagation rate; and the quality of lycoris is guaranteed.

Provided are a lycoris radiata bulb extractive pesticide and a method for preparing an extractive. Lycoris radiata bulbs can be manufactured into the lycoris radiata bulb extractive pesticide in forms of missible oil, microemulsion, wettable powder and the like through certain processing procedures. The lycoris radiata bulb extractive pesticide has good insecticidal ovicidal activities to tetranychus, plutella xylostella, vegetable aphides, armyworms and pine wood nematodes and protective effect in fields and woodlands. The pesticide is remarkable in effect, low in toxicity and residuals, simple in preparing process, low in cost and suitable for popularization and application and accords with the development tendency of existing pesticides, and pests do not easily produce drug resistance.

The invention relates to an ultrasonic coupling agent and a preparation method thereof. The coupling agent comprises the following raw materials of effective components: angelica sinensis, girald daphne barks, lalang grass rhizome, dark plum, Chinese pulsatilla roots, polygonum paleaceum, myrobalan, honeycombs, caesalpinia decapetala roots, lycoris radiata, paris polyphylla, moleplant seeds, folium artemisiae argyi, glycerol, medical purified water and mung bean starch. The coupling agent provided by the invention is prepared by using the modern advanced pharmaceutical technology and made of medicinal materials with proper compatibility, has a better coupling effect and better comfort in use, has the advantages of efficient sterilizing and disinfecting effects, no injury to a human body and a probe, no irritation and sensitization to skin, no pollution to clothes, easiness for cleaning, lower cost, good stability and the like, and can be used for avoiding cross infection during clinical ultrasonic inspection. When used for carrying out ultrasonic inspection, the coupling agent provided by the invention is more remarkable in acoustic transmission effect and clearer in ultrasonic imaging.

The invention discloses a process of extracting dihydrogalanthamine from Lycoris radiata plant, which in detail relates to a method for separating dihydrogalanthamine exquisite from alkaloid extracted from Lycoris radiata plant, and it is a recombined method of enzyme technology and chromatography. It comprises process and condition of extraction and separation of coarse lycorine, then extracting filtering liquid of coarse lycorine, condensing, preparing column material, loading column, eluting, condensing, recrystallizing and getting dihydrogalanthamine with purity of over 99%. The invention is different from method using organic solvent for dihydrogalanthamine extraction, and it can obtain coarse lycorine, coarse galantamin and dihydrogalanthamine with purity over 99%.

The invention discloses a plant powder pesticide and a method for preparing the same. The plant powder pesticide comprises the following plant raw materials in weight portion: 200 to 300 weight portions of macleaya cordata, 300 to 400 weight portions of euphorbia humifusa, 200 to 300 weight portions of polygonum spicy, 50 to 150 weight portions of licorice powder, 300 to 400 weight portions of anisodus mairei, 150 to 250 weight portions of cortex pseudolaricis, 200 to 300 weight portions of cortex moutan, 150 to 250 weight portions of agrimony, and 150 to 250 weight portions of lycoris radiata. The method is relatively simple to operate, is carried out at normal temperature and pressure, and consumes low energy; and various plant raw materials can be combined together to be extracted most properly at the normal temperature, and mutual influences of the components are smaller. The plant powder pesticide has better killing effect on rice leaf insects, cabbage caterpillars, and aphids. The antifeedant rates to pests are more than 96 percent, and the mortality rate is more than 80 percent. The plant powder pesticide adopts a compound preparation of various plant components so that pests are not easy to generate drug resistance generally.

The invention relates to a method for biologically preventing and controlling crop diseases and insect pests, in particular to a method for interplanting lycoris and pyrethrum to prevent and control crop disease and insect pests. The method includes planting the lycoris and the pyrethrum on the peripheries of crop cultivation lands in a matched manner to achieve purposes of preventing and controlling the crop diseases and insect pests. The method has the advantages that ecological environments of the cultivation lands can be improved, the crop diseases and insect pests can be controlled for the cultivation lands without chemical pesticides, accordingly, the amounts of pesticides applied to main crops of the cultivation lands can be greatly reduced, pesticide residues of the crops can be guaranteed against exceeding standards, and the organic crops can be manufactured; the lands can be sufficiently utilized, so that the environment can be beautified.

A preparation method of a traditional Chinese medicine lotion for treating redness-swelling type cellulitis belongs to the technical field of traditional Chinese medicine preparation methods. Currently, antibiotics and sulfanilamide medicaments are generally used for the treatment of redness-swelling type cellulitis, and have the disadvantage of great side effect. The technical scheme of the invention is that the preparation method comprises the following steps: soaking the following 28 herbs of polygonum hydropiper, common lantana leaf, sedum, shrubby breynia, phymatopsis hastata, herba patriniae, changium smyrnioide, hemsleya macrosperma, rhizoma anemarrhenae, radix tinosporae, nasturtium, honeysuckle, bidens biternata, houttuynia, herba lespedezae cuneatae, hypericum perforatum, sedum sarmentosum bunge, sagina subulata, lycoris, patience dock root, radix sophorae flavescentis, balsam pear, folium llicis latifoliae, elephantopus scaber, gentrin knotweed, saxifrage, solanum nigrum and licorice in water, decocting with mild fire, filtering to remove residues so as to obtain the medicinal liquid which is the traditional Chinese medicine lotion for treating redness-swelling type cellulitis. The advantages are that the prepared traditional Chinese medicine is less in toxic and side effect, short in treatment course, and high in cure rate, and can also prevent adverse effect caused by western medicine administration.

The invention relates to a method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis. The method comprises the steps of: (1) based on an MS culture medium, adding 6-BA, NAA, 2, 4-D and other plant growth regulators; (2) selecting basal disc-free Lycoris chinensis scales or aseptically cultured bulblets; (3) controlling the culture room temperature at 23-27DEG C, and conducting illumination for 1000-1200lx; (4) inducing calluses and bulblets, then conducting cutting and transferring them into a proliferation medium to carry out subculture; (5) performing strong seedling culture; (6) conducting rooting culture; and (7) disinfecting seedlings then performing transplanting, keeping a moist environment, and when seedling leaves expand, carrying out normal management. The invention makes use of basal disc-free scales of Lycoris chinensis to establish a rapid propagation system, and has the advantages of fewer materials, high propagation coefficient and the like. The obtained seedlings have consistent genetic basis, stable traits, and tidy growth potential, and have a significant competitive edge in cultivation of new ornamental and medicinal varieties.

A process for extracting the jilkon from shorttube lycoris bulb or snow lotus in order to treat Alzheimer's disease (AD) features that the mixture of polar solvent (methanol, alcohol, propanol, etc) and water is used as carrying agent and the jilkon is extracted by supercritical CO2 extraction.

The invention relates to the technical field of pesticides, and in particular relates to a flower and tree herbicide special for amaryllidaceae and liliaceae. The flower and tree herbicide is prepared from the following raw materials: oriental plane leaves, yellow cinnamon leaves, pine leaf water, bamboo leaves, quick lime and coal. Most of the raw materials of the flower and tree herbicide are natural plants, and the flower and tree herbicide is prepared by fining ingredients and a small amount of ore by utilizing the principle of mutual reinforcement and restriction of the plants. The flower and tree herbicide provided by the invention is safe and low in toxicity, is specially used for killing weeds but capable of keeping flowers and plants, and is particularly effective for flowers and plants such as zephyranthes candida and radix ophiopogonis.

The invention relates to an insecticide composition for preventing and treating bamboo pests as well as an application thereof, and belongs to the technical field of production and processing of natural insecticides. The insecticide composition is characterized by containing 1.5-8 percent of lycoris radiata extracts, 1-6 percent of surfactants, 0.5-3 percent of stabilizing agents and 84-96 percent of water. The insecticide composition for preventing and treating the bamboo pests has the beneficial effects that the insecticide composition is prepared by taking the pure natural lycoris radiata extracts as active ingredients and has strong effects of preventing and treating the bamboo pests; the killing effect on atrachea vulgaris, apamea vepetita comjuncta, otidognathus davidis, bamboo shoot elaterid beetles, bamboo rhamnomia, aphid, Chyliza bambusae Yang et Wang and bipectilus zhejiangensis and the like is strong; the occurrence of bamboo forest diseases and insect pests can be effectively reduced; and meanwhile, the insecticide composition is quick in taking effect, long in duration and is natural without residue. The insecticide composition has the advantages of simplicity and convenience in processing method, low equipment investment, secure raw material source, economy of energy consumption, safety in storage, transportation and application, and low production cost.

The invention relates to a Chinese herb control agent for crop powdery mildew and a preparation method thereof, and belongs to the technical field of ecological pesticide. The Chinese herb control agent for crop powdery mildew comprises Baijiu, sheath of sugarcane, sweet wormwood, tea seed cake, lycoris radiata herb, cannabis sativa leaves, Japanese banana, garlic, mint leaves, cassiabarktree branchlets, longhairy antenoron herb, heartleaf houttuynia, spanishneedles, litsea cubeba, radix bupleuri, purple perilla, common lophatherum herb and pelargonium graveolens. The finished product is obtained by the steps of weighing, mixed grinding, first cooking, second cooking, quality inspection and packaging and the like. The control agent takes Chinese herbs as main raw materials, has the efficacy of bacteriostasis and sterilization and is good in dispersibility, even in dissolution and fast in absorption, crops suffering from powdery mildew recover in three days after being applied with the control agent and are high recovery rate in one week after agent application, pests and diseases can be prevented effectively, the problem of pesticide residue of agricultural products is solved, and yield increase of crops can be realized.

The invention discloses an external remedy for dermatosis, belonging to traditional Chinese medicine domain. Its feature lies in that it is composed of 80-120 portions of lycoris radiata, 50-80 portions of borneol, 50-80 portions of menthol, 50-100 portions of camphor, 100-200 portions of southenwood. The ethanol which is 90-95% of the full weight and the acetic acid which is 5-10% of the full weight is equably mixed to obtain ethanol-acetic acid mixed liquor. The lycoris radiata, borneol, menthol, camphor and southenwood are taken according to the above ratio, and then added to the ethanol-acetic acid mixed liquor until the mixed liquor submerges the crude medicines. After closed immersion for 15-20 days and filtrating, the filtrate is just the said external remedy. The invention has features of remarkable therapeutic efficacy, short period of treatment, fast effect, uneasy to recur.

The invention relates to an in-vitro probulb division growth method for realizing rapid proliferation of Lycoris herbs, which belongs to the technical field of rapid proliferation of plants. The method comprises the following steps: germination culture of seeds; proliferation culture of probulbs; and swelling culture of adventitious buds until substantially-swollen small bulbs are obtained. According to the invention, the probulbs obtained after seed germination are used as explants, and proliferation culture and swelling culture are carried out through basal disc cutting of in-vitro bulbs; so the rate of pollution is substantially reduced; loss of wild seedball resources of the Lycoris herbs is decreased; and a great number germplasm populations with a high growth synchronization degree and consistent properties can be efficiently obtained in a short period.

A mildew-resistant Chinese herbal medicine feed additive is disclosed. The feed additive is characterized by comprising 5-15 parts by weight of liquorice root, 5-10 parts by weight of lycoris radiata, 15-40 parts by weight of seaweed, 1-8 parts by weight of wrinkled giant hyssop, 1-8 parts by weight of clove, 1-6 parts by weight of cortex cinnamomi, 1-5 parts by weight of dried tangerine peel and 1-3 parts by weight of argy wormwood leaves. The feed additive is free of irritation and corrosion, high in stability, long in storage period, free of reactions with feed and free of odour. Palatability and nutrients of feed are not influenced after the feed additive is added into the feed. Synergistic effects of Chinese herbal medicine components can effectively prevent mildew of the feed. The feed additive is low in adding amount, simple in composition, convenient to use and low in cost.

The invention relates to cytochrome P450 reductase as well as coding gene and application thereof. The invention discloses cytochrome P450 reductase 1 of lycoris aurea as an amaryllidaceae plant for the first time; the cytochrome P450 reductase 1 has favorable coenzyme specificity and can be used for assisting a cytochrome P450 enzyme in exerting catalytic activity to modify a synthetic product of a substrate of the cytochrome P450 enzyme in an oxidative manner. The invention also discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier and a host cell for expressing the cytochrome P450 reductase and a method for producing p-coumaric acid.

The invention discloses a method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and the lycoris radiata lectin produced by the method. The lycoris radiata bulbs are taken as raw materials and are extracted and purified by a specific process to form pure lycoris radiata lectin with a single protein band, and the pure lycoris radiata lectin has the apparent molecular weight of 48KD, is protein consisting of four subunits with the same molecular weight, is amorphous powder and has the lycoris radiata lectin content of 90 percent. The method comprises the following process steps of: (1) preparing raw materials; (2) pulping; (3) performing centrifugal separation for the first time; (4) leaching; (5) performing centrifugal separation for the second time; (6) mixing centrifugal separation solutions 1 and 2; (7) performing fractional precipitation; (8) performing centrifugal separation for the third time; (9) performing dialysis demineralizing for the first time; (10) performing electrochromatophoresis separation; (11) performing molecular sieve chromatography; (12) eluting; (13) performing dialysis demineralizing for the second time; (14) performing vacuum concentration; and (15) performing vacuum drying. The invention provides a method capable of extracting the lycoris radiata lectin, lycoris radiata polypeptides and lycoris radiata gelatin and also capable of extracting and separating total alkaloids of lycoris radiata; the process is simple; and the energy is saved and the consumption is reduced.

The invention provides a traditional Chinese medicine for treating arrhythmia due to heart-spleen deficiency and a preparation method thereof. The traditional Chinese medicine for treating the arrhythmia due to heart-spleen deficiency is prepared from the following raw materials: stropanthus divericatus, lycoris, coriaceous knotweed rhizome, celery, wilson begonia rhizome, fragrant sarcococca herb, sambucus adnata wall, mimosa pudica, corallodiscus flabellate, cinnabar, hairyvein agrimony, grass, orchidaceae ludisia, dioscorea pentaphylla, companumoea root, panicled fameflower root, dillseed, madder root and humulus lupulus. The traditional Chinese medicine for treating the arrhythmia due to heart-spleen deficiency has the beneficial effects that the theory of traditional Chinese medicines is utilized, and the advantages of quick effect, definite therapeutic effect, high safety, no toxic or side effect and difficulty in recurrence can be realized when the traditional Chinese medicine provided by the invention is used for treating the arrhythmia due to heart-spleen deficiency.

The invention belongs to the field of tissue culture, and relates to a rootless tissue culture method of lycoris radiata and a lycoris radiata cultivating method. The rootless tissue culture method oflycoris radiata, provided by the invention comprises the steps of performing induction on bulb blocks in induction culture mediums so as to obtain adventitious buds, wherein the induction culture mediums use MS as a base, also comprise 0.5-3.0mg/L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg/L of alpha-naphthaleneacetic acid, 30g/L of cane sugar and/or white sugar and 6-8g/L of agar; and culturing the adventitious buds in propagation culture mediums so as to obtain rootless tissue culture seedlings, wherein the propagation culture mediums use MS as a base and also comprise 3.0-5.0mg/L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg/L of alpha-naphthaleneacetic acid, 0-3mg/L of forchlorfenuron, 30g/L of cane sugar and/or white sugar, 0-1mg/L of thidiazuron and 7-8g/L of agar, and numberof times of propagation culture is 1-5. Through the adoption of the rootless tissue culture method of lycoris radiata provided by the invention, the survival rate is high.