28 results about "Lycoris radiata" patented technology

The invention relates to a phenylpropanoid compound as well as a preparation method and application thereof, in particular to a phenylpropanoid compound extracted from pleione bulbus pseudobulb as well as a preparation method and application thereof, and belongs to the technical field of medicines. The invention provides a method for preparing 10 new compounds from pleione bulbocodioides pseudobulb as a raw material for the first time, systematically evaluates the anti-neuroinflammation activity of pleione bulbocodioides pseudobulb, and clarifies the application of pleione bulbocodioides pseudobulb in development and treatment of central nervous system diseases. According to the invention, abnormally activated BV-2 microglial cells induced by LPS (Lipopolysaccharide) are adopted, and the NO release amount is taken as an index, so that the effect of the compound 1-10 on inhibiting excessive activation of the microglial cells is preliminarily evaluated. The result shows that the novel compounds 1-10 can inhibit the release of LPS-induced BV-2 microglial cell NO. Therefore, the compound prepared in the invention can be applied to development of drugs for treating central nervous system diseases.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention provides a red and blue lycoris radiata tissue culture method, and relates to the technical field of plant propagation, the method sequentially comprises the steps of explant collection and pretreatment, explant disinfection, induction culture, multiplication culture, strong seedling culture and rooting culture, aiming at different requirements of each culture stage, a low-nitrogen / high-nitrogen adaptive culture medium system is customized, and the culture medium system is used for culturing the red and blue lycoris radiata. According to the method, the hormone proportion is accurately regulated and controlled, functional components such as acidolysis casein, coconut juice and activated carbon are added, meanwhile, culture environment parameters are optimized, an asexual propagation mode is adopted, efficient propagation of the lycoris radiata buds can be rapidly achieved, the problem of activity decline after long-term propagation is solved, the risks of pollution, browning and vitrification are effectively reduced, and the survival rate of the lycoris radiata buds is increased. The robustness and transplanting survival rate of tissue culture seedlings are improved, and meanwhile, stable inheritance of excellent characters of varieties is guaranteed.

The invention discloses a method for preparing an in-vitro insect repellent spray for pets by using a lycoris plant extract, which comprises the following steps: S1, raw material pretreatment: cleaning, drying and crushing a lycoris raw material to obtain lycoris powder; s2, extracting the lycoris powder by using an ethanol reflux extraction method to obtain an extraction filtrate; and S3, firstly concentrating the lycoris radiata extract filtrate to obtain a thick lycoris radiata extract concentrate, then performing impurity removal and purification on the concentrate, and after purification is completed, performing concentration again to obtain a pure lycoris radiata extract. The spray has the advantage of being good in parasite expelling effect, and the problems that in the preparation process of an existing pet in-vitro parasite expelling spray, multiple raw materials cannot be evenly mixed conveniently, ingredient distribution is prone to being uneven, and the in-vitro parasite expelling effect on pets is affected are solved.

The application discloses a sophora japonica composition for refractory enteritis and a preparation method and application thereof. The sophora japonica composition for refractory enteritis comprises the following components: sophora japonica, dried orange peel, panax notoginseng, momordica grosvenori and lycoris radiata, and the sophora japonica composition for refractory enteritis is obtained by fermenting plant lactobacillus. The sophora japonica composition for refractory enteritis of the application can obviously increase the amount of duhrlactobacillus, lactobacillus and bifidobacterium and reduce the amount of clostridium in the process of treating inflammatory enteritis; can effectively inhibit the inflammation of colitis, and the mRNA level of inflammatory cytokines TNF-alpha, IL-22, IL-1beta and IL-6 is obviously reduced. The sophora japonica composition can obviously inhibit intestinal inflammation, promote the metabolism of intestinal short-chain fatty acids, increase the thickness of intestinal mucosa, and has obvious curative effects on intestinal microecological homeostasis of inflammatory bowel disease, intestinal inflammation and protection of intestinal mucosa.

The invention provides a traditional Chinese medicine composition with an effect of treating chronic pharyngitis and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from 30-50 parts of phyllanthus emblica, 20-40 parts of oroxylum indicum, 15-25 parts of terminalia chebula, 40-60 parts of roughhaired holly root, 15-35 parts of radix ophiopogonis, 5-15 parts of subprostrate sophora, 25-45 parts of selfheal, 10-30 parts of folium mori, 15-35 parts of chrysanthemum, 5-15 parts of radix scrophulariae, 8-18 parts of scaphium scaphigerum, 150-200 parts of wild chrysanthemum and 40-50 parts of honeysuckle. The preparation method comprises the following steps: (1) pretreatment and enzymolysis of rhizome raw materials; (2) whole-formula mixed extraction; (3) two-stage concentration; and (4) drying. Wild chrysanthemum focuses on clearing away heat and toxic materials and relieving swelling and pain The honeysuckle can help the wild chrysanthemum flower to clear away heat and toxic materials and dispel wind and heat. The two components are matched with the phyllanthus emblica and the like, and are prepared through an ultrasonic enzymolysis-gradient alcohol precipitation process, so that the curative effect is improved, and the recurrence rate is reduced.

The invention discloses a lycoris radiata LlMYB6 gene as well as an expression protein and application thereof, and belongs to the technical field of plant molecular biology. The invention provides a lycoris radiata LlMYB6 gene, the nucleotide sequence of the lycoris radiata LlMYB6 gene is shown as SEQ ID NO.1 in a sequence table, and the amino acid sequence of the lycoris radiata LlMYB6 gene is shown as SEQ ID NO.2 in the sequence table. Expression modes of LlMYB6 in three different development stages of pink lycoris longtube flower slices are quantitatively determined in real time through fluorescence, and functions of the gene are identified through subcellular localization, stable tobacco transformation, dual-luciferase detection and GUS activity detection. It is determined that the transcription factor LlMYB6 can negatively regulate synthesis and accumulation of lycoris radiata anthocyanin by inhibiting expression of the enzyme gene LlPAL, and an effective molecular tool is provided for improving plant flower color through genetic engineering.

The present invention relates to the field of plant cultivation technology, discloses a method for postponing the flowering of Lycoris bulbs, comprising the following steps: S1, 6-9 months, 1-7 days before the Lycoris bulbs bud, the Lycoris bulbs are dug out and transplanted into a planting box for hypophysing, and a planting matrix is ​​filled in the planting box; S2, the planting box is placed in an indoor environment where a temperature of 34-36 DEG C and a relative air humidity of 80-90% are stored, and the storage is 7-60 days; S3, the temperature is adjusted to 23-25 ​​DEG C and the flowers are destroyed, and the relative air humidity is adjusted to 60-75%; S4, after budding, the flowers are transferred to an outdoor natural environment and grown to flowering. The present invention can postpone flowering for 7-60 days after processing the Lycoris bulbs, so that the flowering process can be delayed according to actual exhibition time arrangements, to meet the postponed viewing needs.

The invention provides application and method of geotrichum galactose yeast NBG001 in improving accumulation of alkaloids for lycoris radiata traditional Chinese medicine, and belongs to the technical field of agricultural production, the geotrichum galactose yeast NBG001 is preserved in China Center for Type Culture Collection on July 7, 2025, and the preservation number is CCTCC NO: M 20251547. By inducing the expression of signal molecules and key genes in the lycoris radiate, the simultaneous accumulation of galanthamine, lycorine and narcissus in the lycoris radiate is remarkably improved.

The present invention belongs to the technical field of plant propagation and cultivation, and in particular relates to a basal culture medium, a tissue culture medium, and a regeneration tissue culture method for regenerating long-barreled Lycoris radiata. The present invention provides a tissue culture medium for regenerating long-barreled Lycoris radiata, the tissue culture medium comprising an induction medium, a proliferation medium, a differentiation medium, a bud-strengthening medium, and a seedling-strengthening and rooting medium; in the tissue culture medium provided by the present invention, a modified MS medium and a modified B5 medium are used as the basal culture medium, and the combined action of 2,4-D, 6-BA, IBA, NAA, carbon nanotubes, and casein hydrolyzate facilitates the induction of long-barreled Lycoris radiata callus formation, proliferation, differentiation, and rooting, and sucrose, an energy substance, is combined to jointly improve the propagation coefficient of the long-barreled Lycoris radiata, thereby obtaining a large number of long-barreled Lycoris radiata tissue culture seedlings.

The invention provides a preparation method of lycoris plant root tip chromosomes, and belongs to the technical field of biology. The preparation method of the lycoris plant root tip chromosome comprises the steps of root tip induction culture, root tip collection and pretreatment, enzymolysis treatment, chromosome flaking, microscopic observation and the like. A normal-temperature and low-temperature combined 8-hydroxyquinoline double-stage pretreatment mode is adopted for newborn root tips, and a compound enzymatic hydrolysate and a buffer solution pre-preservation system with an optimized ratio are combined, so that the metaphase division cell proportion and the chromosome image quality are effectively improved. The method has the advantages of simplicity and convenience in operation, high efficiency, strong repeatability and the like, is suitable for preparing chromosomes of different types of lycoris radiate and hybrid materials thereof, and can also be popularized and applied to cytogenetics research of other bulb ornamental plants.

The invention provides an ultralow-temperature preservation method of lycoris bulblets, and belongs to the technical field of plant tissue culture. The method comprises the following steps: 1, treating an explant; 2, establishing a tissue culture system; 3, subculturing and inducing the bulblets; 4, pre-culturing the bulblets; 5, cutting bulblets and fixing loading strips; 6, loading and vitrification treatment; 7, freezing, unfreezing and washing; and 8, recovering culture. The rapid tissue propagation and ultralow-temperature preservation method of the lycoris radiate is convenient and efficient, and has reference significance on ultralow-temperature preservation of the lycoris radiate and other bulb and perennial root plant genetic resources.

This invention belongs to the field of plant propagation and cultivation technology, specifically relating to a tissue culture medium and method for the regeneration of Lycoris radiata. This invention provides a tissue culture medium for Lycoris radiata regeneration, comprising a callus induction medium, a callus proliferation medium, and a callus differentiation medium. In the tissue culture medium and method provided by this invention, single scales are selected as explants, and modified MS medium and B5 medium are used as basal media, with appropriate concentrations of 6-BA, NAA, carbon nanotubes, and acid-hydrolyzed casein added. Combined with suitable temperature and light, this facilitates the induction of Lycoris radiata callus proliferation and differentiation, increases the propagation coefficient of Lycoris radiata, and yields a large number of uniform Lycoris radiata tissue culture seedlings.

The application provides an application and method of Galactomyces geotrichum NBG001 in improving accumulation of medicinal alkaloids in Lycoris radiata, and belongs to the technical field of agricultural production. The Galactomyces geotrichum NBG001 is preserved in China Center for Type Culture Collection on July 7, 2025, and the preservation number is CCTCC NO: M 20251547. Through induction of expression of signal molecules and key genes in Lycoris radiata, simultaneous accumulation of galanthamine, lycoramine and narcissus cyclins in Lycoris radiata is significantly improved.

This invention discloses a transcription factor gene PaZFP36 in *Lycoris radiata* that enhances plant cold resistance and yield, and its application. This invention belongs to the field of biotechnology. The protein PaZFP36 is any one of the following: A1) a protein with the amino acid sequence shown in SEQ ID No:2; A2) a protein obtained by substituting and / or deleting and / or adding amino acid residues to the protein of A1), possessing more than 80% identity and the same function as the protein shown in A1); or a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus. Overexpression in wild-type *Lycoris radiata*. PaZFP36 The gene can significantly improve the cold resistance and yield of 'Liqiu' Napier grass, which has important production application value for creating parent plants with excellent cold resistance and high yield characteristics and for breeding cold-resistant and high-yielding varieties.

The invention belongs to the technical field of biology, and discloses a lycoris radiata meiosis cell protein immunofluorescence staining method, which comprises the following steps: 1) treating a fixed anther to obtain a pollen mother cell in a meiosis period; 2) performing enzyme treatment to decompose cytoplasm of the meiotic cells; (3) carrying out slide preparation and fluorescent staining by using the treated meiotic cells; and 4) observing the positioning signal of the target protein under a fluorescence microscope. The pollen mother cells are treated by using glucanase and the like, so that a thicker callose protective layer on the outer layer of the lycoris radiate pollen mother cells is effectively decomposed, and the binding of an antibody and chromosomes is facilitated; meanwhile, a film preparation method is improved, and expression of fluorescence signals is effectively enhanced. The method is of great significance in researching subcellular localization of proteins with different functions in pollen mother cells of lycoris radiate in a meiosis period by using an immunofluorescence localization technology.

This invention discloses a method for delivering a VIGS vector targeting the flower buds inside the bulb of Lycoris radiata, a method for verifying gene function, and their applications, belonging to the field of biotechnology. The method includes: making 4-6 holes around the root plate of a dormant Lycoris radiata bulb, and 2-4 holes in the central area of ​​the bulb base, reaching the meristematic region of the flower bud; immersing the treated bulb in VIGS infection solution up to the wounds on the root plate; and performing vacuum treatment to ensure effective penetration of the infection solution into the internal flower bud tissue. This invention successfully delivered a silencing vector targeting the LrP5CS1 gene to the flower bud, specifically reducing its expression and obtaining a stable early-flowering phenotype in Lycoris radiata, thus verifying the gene function. Therefore, this invention establishes an efficient delivery platform that can be used for rapid screening and verification of other key genes regulating the flowering period and ornamental traits of Lycoris radiata, providing a novel technical means for the functional study of genes related to the flowering phenotype of Lycoris radiata.

The application discloses a method for saving production cost of lycoris radiata tissue culture seedlings and application thereof, and relates to the technical field of plant tissue culture, and comprises the following steps: taking lycoris radiata bulbs, and cutting the bulbs into four pieces in a cross shape; and culturing and inducing small bulbs to proliferate in a proliferation culture medium containing rice powder under greenhouse conditions; wherein, the composition of the proliferation culture medium comprises conventional MS components and special additives, namely rice powder. The method has the beneficial effect that rice powder is used to replace coconut juice as an exogenous additive for lycoris radiata tissue culture production, the proliferation coefficient is improved, and the production cost is remarkably saved, thereby providing technical support for industrialized production of lycoris radiata seedlings.

The invention relates to the technical field of adhesives, and discloses an environment-friendly lycoris starch-vinyl acetate adhesive and a preparation method thereof.The preparation method comprises the steps that water is added into a reaction kettle and stirred, a defoaming agent and polyvinyl alcohol are added, the mixture is heated to 85 DEG C and then self-heated to 90 DEG C, and heat preservation is conducted; cooling to 83 DEG C, adding an emulsifier and part of an initiator, sealing and starting a condenser; slowly dropwise adding vinyl acetate, maintaining the temperature at 77-80 DEG C, and supplementing the initiator every 30 minutes; after dropwise adding, adding the rest initiator, heating to 86 DEG C, and preserving heat; cooling to 83 DEG C, slowly adding lycoris starch paste, and gelatinizing for 30 minutes; and cooling to 65 DEG C, adding the plasticizer dibutyl phthalate, cooling to 50 DEG C, adding the preservative, stirring for 30 minutes, filtering, and packaging to obtain the finished product adhesive. The lycoris starch is introduced into a polyvinyl alcohol-vinyl acetate system, so that the prepared adhesive has the characteristics of high bonding strength, stable viscosity, slight smell and environmental friendliness.

The invention discloses a VIGS carrier delivery method for targeting a flower bud in a lycoris radiate bulb, a gene function verification method and application thereof, and belongs to the technical field of biology. The method comprises the steps that 4-6 holes are formed in the periphery of a bulb root disc of a short-tube lycoris bulb in the dormant period, 2-4 holes are formed in the center area of the bottom of the bulb, the depth of the holes reaches a flower bud meristem area, the treated bulb is immersed in VIGS infection liquid to the wound of the bulb disc, and vacuumizing treatment is conducted to enable the infection liquid to effectively permeate into and reach the internal flower bud tissue. The silent vector of the targeted LrP5CS1 gene is successfully delivered to the flower bud, the expression of the silent vector is specifically reduced, a stable lycoris radiata early flowering phenotype is obtained, and the function of the gene is verified. Therefore, a set of efficient delivery platform is established and can be used for rapidly screening and verifying other key genes for regulating and controlling the flowering phase and ornamental characters of the lycoris radiate, and a brand new technical means is provided for functional research of genes related to flowering phenotypes of the lycoris radiate.

The invention discloses a method for preparing a pet disinfectant by using a lycoris plant extract, which comprises the following steps: S1, lycoris plant extraction: extracting alkaloid in lycoris plants to prepare an alkaloid solution; s2, antibacterial plant extraction: extracting antibacterial disinfection components in antibacterial plants to prepare a supplementary disinfectant; s3, preparing ingredients of the pet disinfectant, namely preparing a surfactant, a humectant, a pH regulator, a buffering agent and an antistatic agent as the ingredients of the pet disinfectant. The method has the advantages of high efficiency and automation, and solves the problems that the production efficiency of the pet disinfectant is low, the extraction effect is unstable, the production cost is high and the like due to the fact that the existing method for preparing the pet disinfectant from the plant extract is inconvenient to efficiently and automatically extract the plant extract in the using process. And the quality of a final product is influenced.

The invention belongs to the technical field of plant preservation, and particularly relates to a compound preservative for lycoris plant fresh cut flowers as well as a preparation method and application of the compound preservative. The compound preservative is prepared from the following effective components in mass concentration: 150 to 200mg / L of citric acid, 15 to 20g / L of cane sugar, 2 to 3g / L of calcium chloride, 1 to 2g / L of chitosan, 1 to 2ppm of nano-silver colloid and 3 to 5mg / L of gibberellin. According to the invention, the nano-silver colloid, the chitosan and the hormone are combined for post-harvest treatment of lycoris plant cut flowers for the first time; four functions of antibiosis, cell energy stabilization, nutrition supply and hormone regulation are integrated, a multi-factor synergistic mechanism is established, and then the postharvest preservation effect of the lycoris plant cut flowers is remarkably improved. Results of the embodiment show that the composite preservative can prolong the life of the lycoris plant cut flower vase to 6-8 days, the phenomena that the base part of the flower stem is curled and the petals are premature senility are obviously reduced, and the good ornamental value is maintained.

The invention discloses application of lycoris radiata exosomes in preparation of a medicine for treating ectopic ossification, and belongs to the technical field of biological medicine. According to the prepared dried lycoris radiata exosome La-dEXOs, osteoclast differentiation and osteoblast differentiation experiments and animal experiments prove that the prepared La-dEXOs can improve ectopic ossification of rats by promoting osteoclast differentiation and inhibiting osteoblast differentiation. Experimental results show that La-dEXOs has the potential to be developed into related medical products for preventing and treating ectopic ossification.

This invention provides a method for tissue culture of Lycoris radiata. This method constructs a rapid propagation system from explants to complete plants, solving the problem of the lack of large-scale propagation technology for Lycoris radiata and breaking the dependence on imported bulbs. Through self-made culture medium hormone ratios and culture conditions, the bulblet proliferation coefficient reaches 3.6, enabling continuous propagation year-round and rapidly obtaining large quantities of high-quality seedlings to meet the needs of industrial production. Furthermore, the Lycoris radiata tissue culture seedlings produced by this invention have robust root systems and plump bulbs, with an average root length of over 6 cm and an average number of over 6 roots. They grow well after transplanting, providing a high-quality seedling foundation for subsequent landscaping applications and cut flower production. The entire culture process has clear steps and well-defined parameters, requiring no complex equipment or special techniques. It can be easily implemented by those skilled in the art, and the culture conditions are easy to control, highly reproducible, and suitable for large-scale application.

The invention belongs to the field of molecular biology, and discloses a method and a primer group for identifying interspecific distant hybrid filial generation of lycoris radiate and hippeastrum rutilum and application of the method and the primer group. A far-source specific primer group capable of identifying the hybrid offspring of the lycoris radiata and the hippeastrum hippeastrum is screened out, aiming at classification and identification of plants of various types and numbers, accurate identification of the interspecific hybrid offspring of the lycoris radiata and the hippeastrum hippeastrum can be conveniently and quickly realized by adopting the primer group, and both the speed and the effect are better; fluorescence labeling is applied to an SSR molecular labeling system, band information is obtained through the capillary electrophoresis technology, an existing SSR technical system is optimized and improved, and compared with silver staining detection and agarose condensation detection, automatic fluorescence detection has the advantages of being high in throughput and automatic in operation.

The invention relates to a pleione bulbocodioides extract preparation method, a pleione bulbocodioides extract product and application, the preparation method comprises the following steps: crushing pleione bulbocodioides, mixing the crushed pleione bulbocodioides with water, and carrying out ultrahigh pressure treatment to obtain a first mixture; carrying out subcritical extraction on the first mixture, and filtering to obtain an extracting solution; filtering the extracting solution through a nanofiltration membrane to obtain pleione bulbocodioides filtrate; and mixing the pleione bulbocodioides filtrate with a decolorizing agent, filtering, and drying the filtrate to obtain the pleione bulbocodioides decolorizing agent. The method disclosed by the invention is high in extraction efficiency, green and environment-friendly, the obtained extract is rich in active ingredients, can effectively activate the expression of silk polyprotein and paphiopetin, promote the repair of keratinocytes and inhibit sebaceous gland cells from secreting grease, and can maximize the barrier repair and oil control effects of the extract under a specific extraction process.

The use of extracts from plants of the genera *Lycoris* and *Zephyranthes* in the control of rice blast belongs to the field of plant-derived fungicide technology. This paper discloses the uses of extracts from seven *Lycoris* species and one *Zephyranthes* species in the control of rice blast. These extracts can effectively inhibit the mycelial growth and spore germination of *Bacillus oryzae*, and have a good control effect on rice blast. The use of these extracts can delay the development of fungicide resistance in *Bacillus oryzae*.

This invention belongs to the field of biotechnology and discloses a method for immunofluorescence staining of proteins in Lycoris radiata meiotic cells. The method includes the following steps: 1) treating fixed anthers to obtain pollen mother cells in the meiotic stage; 2) treating with enzymes to decompose the cytoplasm of the meiotic cells; 3) preparing slides and performing fluorescent staining on the treated meiotic cells; 4) observing the localization signal of the target protein under a fluorescence microscope. This invention uses dextranase and other enzymes to treat pollen mother cells, effectively decomposing the thick outer callosity protective layer of Lycoris radiata pollen mother cells, which is beneficial for antibody binding to chromosomes; at the same time, it improves the slide preparation method, effectively enhancing the expression of fluorescence signals. This method is of great significance for studying the subcellular localization of proteins with different functions in Lycoris radiata pollen mother cells in the meiotic stage using immunofluorescence localization technology.