80 results about "Regeneration tissue" patented technology

The present patent describes a biocompatible composite made of a first fibrous layer attached to a three-dimensional inter-connected open cell porous foams that have a gradient in composition and / or microstructure through one or more directions. These composites can be made from blends of absorbable and biocompatible polymers. These biocompatible composites are particularly well suited to tissue engineering applications and can be designed to mimic tissue transition or interface zones.

A system for stimulating tissue to relieve pain and repair and / or regenerate tissue comprising a garment that has an electrode, a programmable electrical stimulation device, a time varying electromagnetic field generator, and a source that provides an electrical current. Also provided is a method wherein the electrode provides the user with a stimulating current and the time varying electromagnetic field provides the user with a time varying electromagnetic field either simultaneously, alternating, and / or sequentially.

The present invention relates to a resorbable polymeric mesh implant, that is intended to be used in the reconstruction of soft tissue defects. The mesh implant comprises at least a first and a second material, wherein the second material is substantially degraded at a later point in time than the first material following the time of implantation. The mesh implant is adapted to have a predetermined modulus of elasticity that gradually is decreased until the mesh implant is completely degraded and subsequently resorbed. Due to the gradual decrease in the modulus of elasticity of the inventive mesh implant, the regenerating tissue may gradually take over the load applied to the tissue defect area.

Tissue engineering devices for pelvic floor repair are disclosed. More specifically, tissue engineering devices made of an implant, having a central portion at least partially embedded within a nonwoven felt are disclosed.

The present invention discloses methods and compositions comprising a biocompatible gel that is used in tissue repair and regeneration. Once the gel is administered to a tissue site in need of repair or regeneration, the cells migrate from the gel into the tissue and repair or regenerate the tissue.

The present invention provides methods and compositions for sequentially and separately reducing infection and / or inflammation and regenerating tissue at a lesion site, by contacting the lesion site with a biodegradable scaffold that first delivers one or more agents at the lesion site to reduce infection and / or inflammation and then delivers one or more agents to regenerate tissue at the lesion site after inflammation is reduced.

A crosslinked elastin, a water-soluble crosslinking agent to be used for crosslinking, molded elastin articles, medical instruments and regeneration tissues using the crosslinked elastin, and a surgical therapy method and regeneration treatment wherein the medical instruments are employed. There is provided a biocompatible functional material having elasticity suitable for transplantation into the body without causing detachment of cell adhesion proteins.

The present invention can be used for actual implantation surgery without a scaffold. The present invention provides a synthetic tissue or complex which can be produced by culture and has a high level of differentiation ability. The present invention also provides a therapy and medicament for repairing and / or regenerating tissue using replacement and covering. By culturing cells under specific culture conditions such that medium contains an extracellular matrix synthesis promoting agent, the cells are organized and are easily detached from a culture dish. The present invention was achieved by finding such a phenomenon. In addition, the self contraction of the tissue can be regulated by culturing the tissue in a suspended manner. Therefore, it is possible to regulate the three-dimensional shape of the tissue. The present invention also provides a method for producing an implantable synthetic tissue which does not require a plurality of monolayer cell sheets assembled to form a three-dimensionally structured synthetic tissue. The present invention is characterized by richness in adhesion molecules, nonnecessity of additional fixation at an implantation site, and good biological integration.

A process of tissue regeneration is provided that includes the administration of a stem cell topically to a surface of a tissue. The tissue being in vivo in a subject or in vitro. The tissue has a biocompatible matrix applied around the stem cell and in contact with the surface. After sufficient time, the stem cell infiltrates and regenerates the tissue. A composition is also provided that includes an injured or diseased tissue with a plurality of stem cells proximal to the tissue surface in a biocompatible. A process of increasing a level of cytokines, chemokines, growth factors, anti-apoptotic or anti-necrotic factors in a tissue is provided that includes the administration of a stem cell topically to a surface of a tissue in a biocompatible matrix. After sufficient time, the stem cell releases soluble pro-regenerative factors to increase the levels in the tissue.

The present invention provides compositions and methods for wound healing and tissue regeneration. The compositions of the present invention comprise amniotic membrane of the placenta. In certain embodiments, the composition comprises amniotic membrane powder or solubilized amniotic membrane (SAM). In some aspects, the composition is cell-free and rich in cytokines, extracellular matrix proteins, and other components that improve tissue regeneration. In one aspect, the composition is a hydrogel scaffold that comprises amniotic membrane. The present invention reduces contraction and improves blood vessel development in regenerating tissue.

A process for the preparation of a tissue matrix suitable for regenerative repair of tissues, including contacting an isolated connective tissue with an amount of detergent and an amount of disinfectant to significantly reduce at least one of lipids, phospholipids, nucleic acids, major histocompatibility (MHC) antigens, contaminating microorganisms, and endotoxins. The process further provides for less significant reduction in proteoglycan content while retaining the overall structure of the tissue matrix produced. Processing may further include micronizing the tissue matrix. Also, a tissue matrix having a scaffold portion and non-structural portion, collectively structured to promote cellular infiltration, attachment, and proliferation. The tissue matrix may be in the form of a sheet, thick sheet, or micronized. Also, kits including a prepared tissue matrix for use in regenerative repair, and kits for preparing a tissue matrix.

The present invention discloses biological surgical patch and its preparation process. The biological surgical patch includes one base material of animal membrane material, which is cross-linking fixed with non-aldehyde fixing agent and treated to eliminate antigen. The biological surgical patch made of natural biological material with collagen as main component may be degraded in the degradation speed synchronizing with the growth of the regenerating tissue, and the degraded products are amino acids or polypeptide capable of being absorbed by organism for the regenerative repair of the defected tissue. The present invention has no immunological rejection reaction, high biocompatibility, capacity of inducing tissue regeneration and good mechanical compliance, and can meet the requirement for repairing tissue.

The object of the present invention is to provide: a hybrid material for regeneration of living body tissue, which can retain sufficient mechanical strength for a term needed to regenerate the tissue, and which simultaneously has moderate biodegradation absorbability which enables degradation and disappearance rapidly after the regeneration of the tissue. As means for solving the problems, the hybrid material for regeneration of living body tissue, according to the present invention, comprises a naturally-occurring polymer and a biodegradation-absorbable synthetic polymer, wherein the biodegradation-absorbable synthetic polymer exists in a fibrous form in a sponge including the naturally-occurring polymer, and wherein the naturally-occurring polymer is crosslinked.

The present invention can be used for actual implantation surgery without a scaffold. The present invention provides a synthetic tissue or complex which can be produced by culture and has a high level of differentiation ability. The present invention also provides a therapy and medicament for repairing and / or regenerating tissue using replacement and covering. By culturing cells under specific culture conditions such that medium contains an extracellular matrix synthesis promoting agent, the cells are organized and are easily detached from a culture dish. The present invention was achieved by finding such a phenomenon. In addition, the self contraction of the tissue can be regulated by culturing the tissue in a suspended manner. Therefore, it is possible to regulate the three-dimensional shape of the tissue. The present invention also provides a method for producing an implantable synthetic tissue which does not require a plurality of monolayer cell sheets assembled to form a three-dimensionally structured synthetic tissue. The present invention is characterized by richness in adhesion molecules, nonnecessity of additional fixation at an implantation site, and good biological integration.

The invention provides a medical collagen material, a manufacturing method and the application of the medial collagen material. The medical collagen material is provided with a prismatic artificial ligament / hamstring tendon with seams and a platy film provided with dense pores. The artificial prismatic ligament / hamstring tendon is provided with the longitudinal pore that is specifically sized. The artificial prismatic ligament / hamstring tendon and the platy film are used for connecting and wrapping injured sutured part. The medical collagen material has the characteristics of stable structure, good biocompatibility and the ability of degradation and absorption and can reduce or prevent tissue adhesion.

The invention relates to a biphysic tissue engineering joint scaffold as well as a preparation method and an application thereof. The biphysic tissue engineering joint scaffold consists of a phrenology scaffold and a cartilage scaffold, wherein the cartilage scaffold is a PGA / PLA scaffold; the phrenology scaffold is a PCL / HA scaffold. The invention also provides a biphysic cell-tissue engineering joint scaffold constructor as well as a preparation method and an application thereof. The biphysic tissue engineering joint scaffold as well as the preparation method and the application thereof have the advantages that the biphysic tissue engineering joint scaffold is manufactured by utilizing a CAD / CAM technology, the external form and interior structure are accurate and controllable, the phrenology and the cartilage are matched with each other, the biphysic cell-tissue engineering joint scaffold constructor is formed by compositing seed cells by utilizing a tissue engineering technology, the tissue engineering joint can be regenerated in vivo and has the biological and mechanical biomechanical characteristics of the normal joint, an interface of a regenerated bones and the cartilage is well integrated, and the possibility is provided for performing biological reconstruction through the tissue engineering joint instead of the existing artificial joint.

A crosslinked elastin, a water-soluble crosslinking agent to be used for crosslinking, molded elastin articles, medical instruments and regeneration tissues using the crosslinked elastin, and a surgical therapy method and regeneration treatment wherein the medical instruments are employed. There is provided a biocompatible functional material having elasticity suitable for transplantation into the body without causing detachment of cell adhesion proteins.

The production process of human body tissue engineering carrier frame includes the following steps: firstly, preparing liquor containing tissue engineering carrier frame material and liquid containing growth factor at room temp., according to the user's requirements and previously designed and defined pathway extruding or jetting the above-mentioned two liquors respectively by means of different sprayers in the low-temp. environment of below zero deg.C, and making them form layer-by-layer stacked frozen porous tissue engineering carrier frame, then freeze-dried in freeze-drying machine, removing solvent so as to obtain the invented tissue engineering carrier frame. Its formation process and porosity structure of the frame, and the pore size and its through property can be well controlled,so that it is favorable for internal degradation of carrier frame in human body and nutrition and metabolism of regenerated tissue, and can well retain the bio-activity of biological material.

Methods and compositions for treating diseased or damaged tissue, such as Inflammatory Bowel Disease, e.g., Ulcerative Colitis, include tissue regeneration using stem cells or tissue grafts which stimulate stem cell migration to the damaged tissue. The tissue grafts can be extracellular matrix (ECM) material, such as tissue-specific extracellular matrix (TS-ECM). The methods can also include mucosal resection of the damaged or diseased tissue prior to placement of the graft.

The invention discloses a Chinese material medica preparation for treating bedsore and a preparation method thereof. The amount of components of the preparation is obtained from long-term experience and summarization of the inventor, and the following components have good curative effect by the following weight: astragalus root, panax ginseng, viola herb, dandelion, honeysuckle, frosythia fruit, angelica, white peony root, red salvia root, peach kernel, safflower, purple gromwell root, dragon's blood and pearl powder and elephant skin powder. The preparation method combines the panax ginseng and the astragalus root, and combines the medicines to cause each pharmaceutical efficacy to produce synergetic effect, thus effectively replenishing qi to invigorate the spleen, and supporting the healthy energy. Main functions are supplementing qi and activating blood circulation, clear away heat and toxic materials, removing putrefaction and promoting tissue regeneration, dissipating blood stasis, and promoting regeneration tissue for astringency. Compared with the prior art, the Chinese material medica preparation has advantages of having little side effect, addressing both the symptoms androot causes, and effectively treating bedsore for employing the technical proposal.

A graft containing a scaffold that includes a matrix in which are positioned mesenchymal progenitor cells (MPCs) has the capacity to substantially improve wound healing, including wounds resulting from injury to nerve, bone and vascular tissue. MPCs can be harvested from debrided muscle tissue following orthopaedic trauma. The traumatized muscle-derived progenitor cells are a readily available autologous cell source that can be utilized to effect or improve wound healing in a variety of therapeutic settings and vehicles.

The invention relates to a populus alba * pobulus davidiana dode 1333 tissue culture medium of an pobulus davidiana dode improved variety, which is used for solving a problem that the existing culture medium is poor in in-vitro rapid propagation effect for populus alba* pobulus davidiana dode 1333 regeneration tissue of hybrid offspring pobulus davidiana dode improved variety of pobulus davidiana dode and populus alba. The populus alba* pobulus davidiana dode 1333 tissue culture medium of the pobulus davidiana dode improved variety comprises an adventitious bud differentiation culture medium, a subculture multiplication culture medium and an adventitious bud rooting culture medium, wherein the adventitious bud differentiation culture medium consists of a YS (Eosine Yellowish) culture medium, 6-benzyl amido purine and kinetin; the subculture multiplication culture medium consists of the YS culture medium, the 6-benzyl amido purine and naphthylacetic acid; the adventitious bud rooting culture medium is composed of the YS culture medium and hormone A, wherein the hormone A consists of one or several of indolebutyric acid, indoleacetic acid and naphthylacetic acid in proportion. The populus alba * pobulus davidiana dode 1333 tissue culture medium disclosed by the invention is applied to the plant tissue culture filed.

The invention provides a three-dimensional space cell culture system preparation method. A fine chain is placed in an outer casing pipe, biological degradable materials are melted and then filled in the outer casing pipe, and after the biological degradable materials are solidified and sized, the outer casing pipe and the fine chain are removed, so that a three-dimensional cell culture unit is prepared. The three-dimensional space culture unit prepared by the method can be applied in long-period cell culturing and a large amount of cells generating, the large amount of cells serve as seed cells of tissue engineering, and the three-dimensional space culture unit can be applied in transplant treatment by generating regeneration tissue and micro-organs in a short time.

Proteins which are upregulated in injured or regenerating tissues, as well as the DNA encoding these proteins, are disclosed, as well as therapeutic compositions and methods of treatment encompassing these compounds.

The invention discloses silk fibroin-sodium hyaluronate cross-linked dual-network gel and a preparation method thereof. The method comprises the following steps: catalyzing silk fibroin macromoleculesto generate a cross-linking reaction by using tyrosinase according to the characteristic that tyrosine residue content in silk fibroin is high to generate a first silk fibroin network; using carbodiimide to mediate cross-linking among sodium hyaluronate macromolecules to generate a sodium hyaluronate second network; and mixing the first network and the second network, and generating a cross-linking bond between the first network and the second network by means of mediation of the carbodiimide, thereby preparing the silk fibroin-sodium hyaluronate cross-linking double-network gel. The gel provided by the invention has the characteristics of stable three-dimensional network structure, difficult collapse and disintegration, strong biodegradation resistance and good biocompatibility, and canbe widely used for tissue filling, tissue regeneration guidance, tissue engineering scaffolds, drug carriers and the like.

Apparatuses, systems, and methods for enhancing bone or soft tissue regeneration are provided. For example, a conduit, having one or more segments, can originate at a tissue regeneration site and can have a first opening to promote physiological signals to enter the conduit and transit to a second opening that penetrates a histologically rich source of multipotent mesenchymal cells, promoting the multipotent mesenchymal cells to produce tissue regeneration response products, the response products transiting through the second opening to egress at the first opening of the conduit, and promoting tissue regeneration at the tissue regeneration site. Transit of the physiological signals and the tissue regeneration response products is promoted through physical and / or chemical means, as is promotion of the anatomical functionality of the regenerated tissue. For example, a number of the segments of the conduit can be formed at least partially from an osteoceramic material.

The invention relates to a Chinese medicinal composition for treating dental ulcers, which belongs to the field of traditional Chinese medicines. The Chinese medicinal composition for treating dental ulcers is prepared from the following raw medicinal materials in portions by weight: 10-120 portions of indigo naturalis, 5-30 portions of calculus bovis, 5-15 portions of gallnut, 5-15 portions of borneol, 2-10 portions of chalcanthite, 1-5 portions of coptis chinensis and 1-5 portions of field mint. In the invention, the Chinese medicinal composition is prepared into other preparation formulation on the basis of fundamental principles of clearing heat, detoxicating, reducing swelling, relieving pain and promoting regeneration tissue for astringency. According to the clinical application, the Chinese medicinal composition has no any side effect and has a reliable therapeutic effect.

Provided are mammalian cells comprising a recombinant sonic hedgehog (SHH) gene such that a recombinant SHH protein can be expressed by the cell. Also provided are matrices suitable for applying to a tissue defect. Additionally provided are tissue regeneration compositions. Methods of regenerating tissue at the site of a tissue defect in a mammal are also provided.

Proteins which are upregulated in injured or regenerating tissues, as well as the DNA encoding these proteins, are disclosed, as well as therapeutic compositions and methods of treatment encompassing these compounds.