Silk fibroin-sodium hyaluronate cross-linked dual-network gel and preparation method thereof

A technology of sodium hyaluronate and silk fibroin, which is applied in the field of silk fibroin-sodium hyaluronate cross-linked double network gel and its preparation, can solve the problems of ineffectiveness of the gel, collapse and disintegration of the three-dimensional network, and the like. The effect of strong adhesion, firm network structure and excellent biocompatibility

Active Publication Date: 2020-07-24
苏州苏豪生物材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the network inside the gel is formed by mixing and cross-linking silk fibroin and sodium hyaluronate, when the sodium hyaluronate component is first degraded, it is easy to cause the collapse and disintegration of the overall three-dimensional network of the gel, making the gel useless

Method used

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  • Silk fibroin-sodium hyaluronate cross-linked dual-network gel and preparation method thereof
  • Silk fibroin-sodium hyaluronate cross-linked dual-network gel and preparation method thereof
  • Silk fibroin-sodium hyaluronate cross-linked dual-network gel and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0027] After washing, degumming, dissolving and dialysis, the silkworm cocoon shells were diluted with purified water to adjust the concentration to obtain an aqueous silk fibroin protein solution with a concentration of 20 mg / mL.

[0028] Add the 500 U / mL tyrosinase solution and hydrogen peroxide slowly into the silk fibroin protein aqueous solution at 30°C respectively, and stir slowly while adding, every 100 mL of silk fibroin protein solution contains tyrosine The amount of acidase solution added was 15 mL, and that of hydrogen peroxide was 100 μL. The temperature was maintained at 30° C., and the reaction was stirred for 60 minutes to obtain the first component.

[0029] At room temperature, dissolve sodium hyaluronate with an average molecular weight of 1000 kDa in 50 mmol / L 2-morpholineethanesulfonic acid (MES) solution, and stir slowly while dissolving to make the sodium hyaluronate in the solution The concentration is 8 mg / mL, and N-hydroxysuccinimide (NHS) and 1-(3-...

Embodiment 2

[0041] After the silkworm raw silk was degummed, dissolved, and dialyzed, it was diluted with purified water to adjust the concentration to obtain an aqueous silk fibroin protein solution with a concentration of 2 mg / mL.

[0042] At room temperature, dissolve tyrosinase in purified water, stir slowly while dissolving, and prepare a tyrosinase solution with a concentration of 500 U / mL.

[0043] Add the tyrosinase solution and hydrogen peroxide dropwise to the silk fibroin protein aqueous solution at a temperature of 15°C, and stir slowly while adding the solution. The amount of the tyrosinase solution added per 100 mL silk fibroin protein solution is 25 mL, the dropwise volume of hydrogen peroxide is 450 μL. The temperature was maintained at 15°C and the reaction was stirred for 180 minutes. Get the first component.

[0044] At room temperature, dissolve sodium hyaluronate with an average molecular weight of 2000 kDa in 50 mmol / L MES solution, stir slowly while dissolving, so...

Embodiment 3

[0048] After the silkworm raw silk was degummed, dissolved, and dialyzed, it was diluted with purified water to adjust the concentration to obtain an aqueous silk fibroin protein solution with a concentration of 38 mg / mL.

[0049] Add the 500 U / mL tyrosinase solution and hydrogen peroxide slowly into the silk fibroin protein aqueous solution at a temperature of 50 °C respectively, and stir slowly while adding, every 100 mL of silk fibroin protein solution contains The dropping volume of acidase solution was 2 mL, and the dropping volume of hydrogen peroxide was 15 μL. The temperature was maintained at 50°C and the reaction was stirred for 120 minutes. Get the first component.

[0050] At room temperature, dissolve sodium hyaluronate with an average molecular weight of 400 kDa in 50 mmol / L MES solution, stir slowly while dissolving, so that the concentration of sodium hyaluronate in the solution is 20 mg / mL, and then NHS and EDC solutions were added dropwise into the solution...

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Abstract

The invention discloses silk fibroin-sodium hyaluronate cross-linked dual-network gel and a preparation method thereof. The method comprises the following steps: catalyzing silk fibroin macromoleculesto generate a cross-linking reaction by using tyrosinase according to the characteristic that tyrosine residue content in silk fibroin is high to generate a first silk fibroin network; using carbodiimide to mediate cross-linking among sodium hyaluronate macromolecules to generate a sodium hyaluronate second network; and mixing the first network and the second network, and generating a cross-linking bond between the first network and the second network by means of mediation of the carbodiimide, thereby preparing the silk fibroin-sodium hyaluronate cross-linking double-network gel. The gel provided by the invention has the characteristics of stable three-dimensional network structure, difficult collapse and disintegration, strong biodegradation resistance and good biocompatibility, and canbe widely used for tissue filling, tissue regeneration guidance, tissue engineering scaffolds, drug carriers and the like.

Description

technical field [0001] The invention relates to the field of biomedical polymer gel materials, in particular to silk fibroin-sodium hyaluronate cross-linked double network gel and its preparation method, which can be used for tissue filling, guiding tissue regeneration, cell culture carrier, tissue engineering scaffold and Drug controlled release carrier, etc. Background technique [0002] Polymer hydrogel, as a kind of highly water-absorbing and high-water-retaining material with a hydrophilic three-dimensional network structure, is widely used in tissue filling in the field of cosmetic plastic surgery, guided regeneration of defective tissue in orthopedic surgery, tissue engineering scaffolds, and controlled drug release. carrier etc. [0003] Sodium hyaluronate is one of the main components of the natural extracellular matrix and widely exists in the connective tissue, joint lubricating fluid and cartilage of vertebrates. Sodium hyaluronate is a linear polysaccharide co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/12A61L27/52A61L27/58A61L27/22A61L27/20C08L89/00C08L5/08
CPCC08J3/246C08J3/243C08J3/12A61L27/52A61L27/58A61L27/227A61L27/20C08J2389/00C08J2405/08C08J2305/08C08J2489/00C08L89/00C08L5/08
Inventor 郭志方陶伟
Owner 苏州苏豪生物材料科技有限公司
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