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A kind of silk fibroin-sodium hyaluronate cross-linked double network gel and preparation method thereof

A technology of sodium hyaluronate and silk fibroin, which is applied in the field of silk fibroin-sodium hyaluronate cross-linked double network gel and its preparation, can solve problems such as the collapse and disintegration of the three-dimensional network, and the loss of effectiveness of the gel, and achieve The effect of firm network structure, strong adhesion ability and excellent biocompatibility

Active Publication Date: 2022-04-12
苏州苏豪生物材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the network inside the gel is formed by mixing and cross-linking silk fibroin and sodium hyaluronate, when the sodium hyaluronate component is first degraded, it is easy to cause the collapse and disintegration of the overall three-dimensional network of the gel, making the gel useless

Method used

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  • A kind of silk fibroin-sodium hyaluronate cross-linked double network gel and preparation method thereof
  • A kind of silk fibroin-sodium hyaluronate cross-linked double network gel and preparation method thereof
  • A kind of silk fibroin-sodium hyaluronate cross-linked double network gel and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] After washing, degumming, dissolving and dialysis, the silkworm cocoon shell was diluted with purified water to adjust the concentration to obtain an aqueous solution of silk fibroin with a concentration of 20 mg / mL.

[0028] The tyrosinase solution with a concentration of 500 U / mL and hydrogen peroxide were slowly added dropwise to the mulberry silk fibroin aqueous solution at a temperature of 30 °C, and the mulberry silk fibroin protein solution was slowly stirred while adding dropwise. The dropwise volume of acid enzyme solution was 15 mL, and the dropwise volume of hydrogen peroxide was 100 μL. The temperature was maintained at 30°C, and the reaction was stirred for 60 minutes to obtain the first component.

[0029] At room temperature, sodium hyaluronate with an average molecular weight of 1000 kDa was dissolved in 50 mmol / L 2-morpholineethanesulfonic acid (MES) solution, and slowly stirred while dissolving, so that the sodium hyaluronate in the solution was dissol...

Embodiment 2

[0041] After the silkworm raw silk was degummed, dissolved, and dialyzed, it was diluted with purified water to adjust the concentration to obtain an aqueous silk fibroin protein solution with a concentration of 2 mg / mL.

[0042] At room temperature, dissolve tyrosinase in purified water, stir slowly while dissolving, and prepare a tyrosinase solution with a concentration of 500 U / mL.

[0043] Add the tyrosinase solution and hydrogen peroxide dropwise to the silk fibroin protein aqueous solution at a temperature of 15°C, and stir slowly while adding the solution. The amount of the tyrosinase solution added per 100 mL silk fibroin protein solution is 25 mL, the dropwise volume of hydrogen peroxide is 450 μL. The temperature was maintained at 15°C and the reaction was stirred for 180 minutes. Get the first component.

[0044] At room temperature, dissolve sodium hyaluronate with an average molecular weight of 2000 kDa in 50 mmol / L MES solution, stir slowly while dissolving, so...

Embodiment 3

[0048] After the silkworm raw silk was degummed, dissolved, and dialyzed, it was diluted with purified water to adjust the concentration to obtain an aqueous silk fibroin protein solution with a concentration of 38 mg / mL.

[0049] Add the 500 U / mL tyrosinase solution and hydrogen peroxide slowly into the silk fibroin protein aqueous solution at a temperature of 50 °C respectively, and stir slowly while adding, every 100 mL of silk fibroin protein solution contains The dropping volume of acidase solution was 2 mL, and the dropping volume of hydrogen peroxide was 15 μL. The temperature was maintained at 50°C and the reaction was stirred for 120 minutes. Get the first component.

[0050] At room temperature, dissolve sodium hyaluronate with an average molecular weight of 400 kDa in 50 mmol / L MES solution, stir slowly while dissolving, so that the concentration of sodium hyaluronate in the solution is 20 mg / mL, and then NHS and EDC solutions were added dropwise into the solution...

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Abstract

The invention discloses a silk fibroin-sodium hyaluronate cross-linked double network gel and a preparation method thereof. Taking advantage of the high content of tyrosine residues in silk fibroin, use tyrosinase to catalyze the cross-linking reaction between silk fibroin macromolecules to generate the first network of silk fibroin; use carbodiimide to mediate transparency The cross-linking between sodium hyaluronate macromolecules generates the second network of sodium hyaluronate; after mixing the first network and the second network, relying on the mediation of carbodiimide to generate the second network between the first network and the second network Cross-linked bonds, thus making silk fibroin-sodium hyaluronate cross-linked double network gel. The gel provided by the invention has a stable three-dimensional network structure, is not easy to collapse and disintegrate, has strong biodegradation resistance, and has good biocompatibility, and can be widely used in tissue filling, guiding tissue regeneration, tissue engineering scaffolds, and drug carriers.

Description

technical field [0001] The invention relates to the field of biomedical polymer gel materials, in particular to a silk fibroin-sodium hyaluronate cross-linked double network gel and a preparation method thereof, which can be used for tissue filling, guiding tissue regeneration, cell culture carriers, tissue engineering scaffolds and Drug controlled release carrier, etc. Background technique [0002] As a class of high water absorption and high water retention materials with a hydrophilic three-dimensional network structure, polymer hydrogels are widely used in tissue filling in the field of cosmetic surgery, guided regeneration of defective tissue in orthopedics, tissue engineering scaffolds, and controlled drug release. carrier etc. [0003] Sodium hyaluronate is one of the main components constituting the natural extracellular matrix and is widely present in the connective tissue, joint lubricating fluid and cartilage of vertebrates. Sodium hyaluronate is a linear polysa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J3/12A61L27/52A61L27/58A61L27/22A61L27/20C08L89/00C08L5/08
CPCC08J3/246C08J3/243C08J3/12A61L27/52A61L27/58A61L27/227A61L27/20C08J2389/00C08J2405/08C08J2305/08C08J2489/00C08L89/00C08L5/08
Inventor 郭志方陶伟
Owner 苏州苏豪生物材料科技有限公司
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