Biphysic tissue engineering joint scaffold as well as preparation method and application thereof
A tissue engineering and joint technology, applied in the field of tissue engineering joint biphasic scaffold and its preparation, can solve the problem of no breakthrough progress in tissue engineering joint regeneration, and achieve good integration effect.
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[0054] 1. Materials and Methods
[0055] 1.1 Overall experimental design
[0056] Twenty 8-week-old nude mice (Shanghai Experimental Animal Center, Chinese Academy of Sciences) were randomly divided into two groups: the experimental group (n=10), and the control group (n=10); Normal control group (n=10). In the experimental group, the cell-scaffold compound was subcutaneously implanted on the back of nude mice, and in the control group, a scaffold without composite cells was subcutaneously implanted on the back of nude mice. All animal experiments strictly complied with the management regulations on the use of experimental animals of Shanghai Jiaotong University School of Medicine and obtained consent. For the overall experimental design, see figure 1 .
[0057] 1.2 Material Characterization
[0058] As the PLA content increased from 0% to 30%, the compressive modulus of polyglycolic acid / polylactic acid (PGA / PLA) scaffolds gradually increased, whereas the chondrocyte se...
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