125 results about "Cellular formation" patented technology

A method of forming an array of viable cells is carried out by ink-jet printing a cellular composition containing said cells on a substrate. At least two different types of viable mammalian cells are printed on the substrate, the at least two different types of viable mammalian cells selected to together form a tissue. In some embodiments at least three or four different viable mammalian cells are printed on the substrate, the cells selected to together form a tissue. In some embodiments one of the viable mammalian cell types is a stem cell. In some embodiments the method further comprises printing at least one support compound on the substrate, the support compound selected to form a tissue together with said cells. In some embodiments the method further comprises printing at least one growth factor on the substrate, the growth factor selected to cause the cells to form a tissue.

Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby allowing more accurate analysis and classification of circulating tumor cells (CTC) and, ultimately, of tumor burdens in cancer patients. Stabilization of specimens is particularly desirable in protocols requiring rare cell enrichment from blood specimens drawn from cancer patients. Exposure of such specimens to potentially stressful conditions encountered, for example, in normal processing, mixing, shaking, delays due to transporting the blood, has been observed to not only diminish the number of CTC but also to generate debris and aggregates in the blood specimens that were found to interfere with accurate enumeration of target cells, if present. Stabilizers are necessary to discriminate between in vivo CTC disintegration and in vitro sample degredation.

An image data processing system with computing means for the automatic segmentation of a treelike tubular structure in a 3-D image comprising: means (20) for computing a treelike center path of the tubular tree-like structure; means (21) for dividing the treelike center path of the tubular treelike structure into segments formed of points; means (40) for generating generic cylindrical meshes formed of cells, for individual segments of the tree-like center path; means (50) for fusing generic cylindrical meshes by two.

A system and methodology are disclosed for forming a passive layer on a conductive layer, such as can be done during fabrication of an organic memory cell, which generally mitigates drawbacks inherent in conventional inorganic memory devices. The passive layer includes a conductivity facilitating compound, such as copper sulfide (Cu2S), which is generated from an upper portion of a conductive material. The conductive material can serve as a bottom electrode in the memory cell, and the upper portion of the conductive material can be transformed into the passive layer via treatment with a plasma generated from fluorine (F) based gases.

Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and / or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.

A method and apparatus for a novel adaptive aerostructure is presented that relies on certified aerospace materials and can therefore be applied in conventional passenger aircraft. This structure consists of a honeycomb material which cells extend over a significant length perpendicular to the plane of the cells. Each of the cells contains an inelastic pouch (or bladder) that forms a circular tube when the cell forms a perfect hexagon. By changing the cell differential pressure (CDP) the stiffness of the honeycomb can be altered. Using an external force or the elastic force within the honeycomb material, the honeycomb can be deformed such that the cells deviate from their perfect-hexagonal shape. It can be shown that by increasing the CDP, the structure eventually returns to a perfect hexagon. By doing so, a fully embedded pneumatic actuator is created that can perform work and substitute conventional low-bandwidth flight control actuators. It is shown that two approaches can be taken to regulate the stiffness of this embedded actuator.

Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and / or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.

The present invention relates to a method of culturing peripheral lymphoid organ cells on a three-dimensional scaffolding which is covered or surrounded with culture medium, under conditions effective to generate and maintain mature and functional lymphoid cells, where the three-dimensional scaffolding allows cells in the culture medium to have cell to cell contact in three dimensions. The present invention also provides methods of screening for vaccine candidates for efficacy in eliciting an immune response, identifying genes or proteins which are related to peripheral lymphoid organ cell formation or function, screening for drugs effecting peripheral lymphoid organ cell maturation, treating a patient for a disease condition using antigen-specific lymphoid cells, and effecting gene expression of peripheral lymphoid organ cells.

An object of the present invention is to provide a method of producing carbon particles for an electrode, the carbon particles being highly suitable for use as an electrode material, and being able to achieve a high charge / discharge efficiency and durability when used as a negative-electrode material in lithium-ion secondary batteries.The present invention provides a method of producing carbon particles for an electrode, each containing particles of a metal capable of forming an alloy with lithium, being formed by an aggregation of numerous fine particles composed of carbon, and having a hollow open-cell structure in which cells among the fine particles form a plurality of interconnected pores. The method includes the steps of: mixing together a monomer having a low compatibility with a polymer to be formed, an organic solvent having a low compatibility with the polymer to be formed, and particles of a metal capable of forming an alloy with lithium, to prepare a monomer-containing mixture; dispersing the monomer-containing mixture in an aqueous phase to prepare a suspension containing, dispersed therein, oil droplets of the monomer-containing mixture; polymerizing the oil droplets in the suspension to prepare resin particles; and curing the resin particles.

Methods, compositions and kits for concentrating or separating cells containing target nucleic acid are disclosed, especially m mixtures containing the cells and other components such as impurities. The methods can keep a large proportion of the cells intact, allowing the cells to be employed after separation (e.g. cultured) and / or which facilitates the recovery of nucleic acid from the cells. The method employs flocculating agents, such as polyamines or cationic detergents, to form complexes with cells causing them to aggregate and so separated from other components of the mixture. Conveniently, the separation of the aggregated cells can be effected with a solid phase which is capable of binding the cells, such as magnetic beads or filters.

Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and / or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.

New biologically active compounds are described which inhibit the cellular formation of niacinamide mononucleotide, and essential intermediate of the NAD(P) biosynthesis in the cell. These compounds can represent the active ingredient of a pharmaceutical composition for the treatment of cancers, leukaemias or for immunosuppression. Furthermore, screening methods are described as a tool for detecting the above active compounds, and for examination of a given cell type for its dependency on niacinamide as a precursor for NAD synthesis.

A therapeutic product formed from a high concentration of white blood cells having a high degree of cell viability. The white blood cells are sequestered from their normal population presence in whole blood by placing the blood into a container and preventing coagulation of the blood, separating the blood into two components, one of which is extremely rich in white blood cells through the use of a reagent and centrifugation, sequestering the white cell concentration, and freezing the white cells.

The present inventors discovered that by forming a large immune complex comprising antigens containing two or more antigenic binding units (epitopes) and two or more antigen-binding molecules (for example, antibodies), elimination from the plasma of the antigens containing two or more antigenic binding units can be accelerated. Moreover, they found that by using this characteristic and by further using antigen-binding molecules having an ion-dependent antigen-binding activity, elimination of the antigens can further be accelerated and the above problem can be solved.

Provided is a cell analysis method in a cell analysis device D that uses an optical path length image of a cell colony formed of a large number of cells to analyze the cell colony, the method comprising: acquiring the optical path length image of the cell colony by an acquisition unit of the cell analysis device; extracting a circular shape corresponding to a cell nucleus of the cell in the acquired optical path length image by an extraction unit of the cell analysis device extracts; comparing an inner optical path length of the extracted circular shape and an outer optical path length of the extracted circular shape by a comparison unit of the cell analysis device extracts; and analyzing the cell colony based on the comparison result by analysis unit of the cell analysis device.

The invention relates to a biphysic tissue engineering joint scaffold as well as a preparation method and an application thereof. The biphysic tissue engineering joint scaffold consists of a phrenology scaffold and a cartilage scaffold, wherein the cartilage scaffold is a PGA / PLA scaffold; the phrenology scaffold is a PCL / HA scaffold. The invention also provides a biphysic cell-tissue engineering joint scaffold constructor as well as a preparation method and an application thereof. The biphysic tissue engineering joint scaffold as well as the preparation method and the application thereof have the advantages that the biphysic tissue engineering joint scaffold is manufactured by utilizing a CAD / CAM technology, the external form and interior structure are accurate and controllable, the phrenology and the cartilage are matched with each other, the biphysic cell-tissue engineering joint scaffold constructor is formed by compositing seed cells by utilizing a tissue engineering technology, the tissue engineering joint can be regenerated in vivo and has the biological and mechanical biomechanical characteristics of the normal joint, an interface of a regenerated bones and the cartilage is well integrated, and the possibility is provided for performing biological reconstruction through the tissue engineering joint instead of the existing artificial joint.

The subject invention concerns novel and translatable materials and methods for expansion of stem cells, such as mesenchymal stem cells (MSC), that significantly improve translational success of the cells in the treatment of various conditions, such as stroke. The subject invention utilizes cell self-aggregation as a non-genetic means to enhance their therapeutic potency in a microcarrier bioreactor. The subject invention integrates a cell aggregation process in a scalable bioreactor system. In one embodiment of the method, thermally responsive microcarriers (TRMs) are utilized in conjunction with a bioreactor system. Cells are cultured in a container or vessel in the presence of the TRMs wherein cells adhere to the surface of the TRMs. Once cells are adhered to the TRMs they can be cultured at a suitable temperature for cell growth and expansion, e.g., at about 37° C. After a period of time sufficient for cell growth and expansion on the TRMs, the cell culture temperature is reduced so that the cells detach from the TRMs. The detached cells are allowed to form cell clusters that are then cultured under conditions such that the clusters aggregate to form 3D aggregates. The 3D aggregates can be collected and treated to dissociate the cells (e.g., using enzymatic treatment, such as trypsinization). Dissociated cells can then be used for transplantation in methods of treatment or for in vitro characterization and study.

The invention belongs to the field of medical equipment, and more specifically relates to a tissue engineering acellular vascular scaffold preparation method. The method comprises the following steps:1) preparing a spinning fiber skeleton; winding the spinning fibers through a spinning technology at a receiver, and interlacing the spinning fibers up and down to form a cylindrical structure; 2) implanting the spinning fiber skeleton obtained in the step 1) under animal skin, and finally forming the host cell and extracellular matrix-coated tissue engineering vascular scaffold; and 3) removingthe receiver, and removing cells of the tissue engineering vascular scaffold. The constructed fiber skeleton is moved under the animal skin, the tissue vascular scaffold formed by migration cells dueto an immunization mechanism is constructed in the host animal body, accellular processing is carried out, the netted scaffold is kept, the blood vessel mechanical properties can be improved, an immunization reaction after transplantation can be reduced, the extracellular matrix is fully used, and good environment is provided for cell regeneration.

An automated cell growth / migration detection system includes: a container for containing a cell growth / migration matrix / medium into which deposited cells form a cell surface; an image sensor for capturing images; an actuator for incrementally varying distance between the image sensor lens and the cell surface such that the images correspond to varying imaging depths; and an image data processor for processing the images to determine cell growth / migration. An automated cell growth / migration detection method includes: capturing a first image series of a cell surface within a first imaging cycle corresponding to a sequence of imaging depths between the cell surface and an image sensor; capturing an additional image series of the cell surface within each of at least one additional imaging cycle and corresponding to the same sequence of imaging depths; processing each image series for each imaging cycle to determine a clearest-looking image; and determining cell growth / migration from the clearest-looking image.

The invention relates to a preparation method of high gamma-aminobutyric acid tea. The preparation method comprises the following steps: withering fresh Cerasus humilis leaves, de-enzyming, twisting, carrying out primary baking, carding, drying, screening and carrying out other steps to obtain the high gamma-aminobutyric acid tea. The high gamma-aminobutyric acid tea can lower the blood pressure, improve sleeping, strengthen memory, recompose the nerve and resist anxiety, has the function of preventing and treating epilepsy, and can postpone cerebrum senility and regulate the male reproductive function. The high gamma-aminobutyric acid tea contains high free valine, isoleucine, leucine and various other human necessary branched-chain amino acids, and can provide energy reserve for long-time endurance exercises and promote the recovery of sports fatigue. The high gamma-aminobutyric acid tea contains more asparagine, and can form biochemical hindrance for cancerated cells in vivo, thereby inhibiting the growth and reproduction of cancer cells and having synergistic and attenuating effects on chemotherapy of malignant tumors.

In some aspects, cultures of neurons derived from human induced pluripotent stem cells (iPS cells) that exhibit synchronous firing of neural networks are provided. In some embodiments, neuronal activity of the cultures may be detected or measured using a multi-electrode array.

Provided is a novel method capable of manufacturing an artificial skin model including dendritic cells. A method for manufacturing an artificial skin model includes the following: forming a dermal tissue layer by culturing coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component, so that the coated cells are layered; forming a basal layer including type IV collagen on the dermal tissue layer by bringing type IV collagen into contact with the dermal tissue layer; and forming an epidermal layer by arranging epidermal cells on the basal layer. At least one of the dermal tissue layer and the epidermal layer includes dendritic cells.

An MRAM array is formed of MTJ cells shaped so as to have their narrowest dimension at the middle of the cell. A preferred embodiment forms the cell into the shape of a kidney or a peanut. Such a shape provides each cell with an artificial nucleation site at the narrowest dimension, where an applied switching field can switch the magnetization of the cell in manner that is both efficient and uniform manner across the array.

The invention discloses a preparation method of a tissue engineering acellular vascular scaffold. Enhanced tissue engineering blood vessels without immunogenicity can be obtained by embedding micron high-molecular polymer scaffold into subcutaneous part or abdominal cavity of a host and performing acellular treatment on the blood vessel scaffold wrapped by utilizing a host immune protection mechanism. The preparation method has the advantages that deficient mechanical performance of the tissue engineering acellular blood vessel can be solved, and the mechanical performance of the original tissue engineering acellular vascular scaffold can be fully improved through a multilayer cross-linked mesh type high-molecular scaffold; the problem of immunogenicity of the tissue engineering blood vessel in allotransplantation can be solved, the scaffold blood vessel formed by migrating cells caused by immunologic mechanism is established in a host animal body, the transplanted immunoreaction can be reduced through acellular treatment, extracellular matrix is fully utilized to provide a good environment for cell regeneration; and different acellular vascular scaffolds can be prepared through designing scaffolds of different shapes and sizes of by utilizing the method, so as to be applied under different blood vessel transplanting conditions.

An integrated mobile thermal treatment system and method is disclosed for processing organic material. In a first mode of operation, the system includes all process components necessary to thermally treat organic material to achieve cell lysing and cell formation integrated in a common skid-assembled unit. In a second mode of operation, the system additionally includes pre-slurrying, pressurization, selective solids separation, and / or post-dewatering process components to minimize feed volume by pre-slurrying, deliver feed at the necessary treatment pressure, thermally treat organic material to achieve cell lysing and cell formation integrated in a separate or common skid-assembled unit, and produce a dewatered sludge cake suitable for disposal or use as a fertilizer of fuel.

The invention provides a method for preparing induced pluripotent stem cells and a composition used in the method. The method comprises the following step: introducing a composition of promoting induced pluripotent stem cells to form into a body cell, wherein the composition comprises (i) a c-Jun antagonist, and a group of factors selected from the following seven groups of factors (1) Sox2, Klf-4 and c-Myc, (2) Klf4 and c-Myc, (3) Oct3 / 4, Klf4 and c-Myc, (4) Sox2, Nanog and Lin28, (5) Oct3 / 4, Nanog and Lin28, (6) Oct3 / 4, Klf and Sox2 and (7) Klf4 and Sox2; or (ii) a c-Jun antagonist, Jhdm1b and Id1, and at least one of Glis1, Sall4 or Lrh1; or (iii) a c-Jun antagonist, Jhdm1b and Id1, and at least one of Oct4, Klf4, Sox2, Lin28, Esrrb, Lef1, Utf1 or miRNA C. Through the method disclosed by the invention, the induced pluripotent stem cells can be successfully prepared; the obtained induced pluripotent stem cells are good in quality; and no abnormal chromosome is generated.

A means for following the growth of experimental neoplasms involves administering recombinant tumor cells containing an expression construct encoding a secretable marker to an experimental animal and measuring secreted marker in the urine of animals bearing tumors formed by such recombinant tumor cells. Urinary marker levels are quantitatively related to tumor loads. Urinary marker can be detected before tumors are grossly visible or clinically apparent. Marker levels decrease following surgical excision or chemotherapeutic treatment, with an estimated half-life of 11 hours. This approach is applicable to the study of many experimental tumor systems.

Plastic material and nucleating agent are fed into an extruder, and the plastic material is subjected to cell forming. The substances in question are extruded into a plastic film preform, simultaneously cooling the material to prevent foaming. The film preform is stretched in such a way that cavitation bubbles are generated in the film. The cell forming takes place in connection with stretching and relaxation. In other words, in the solution the cell forming is caused to take place when the plastic is not in a molten state.