Low-temp. extrusion/jet-stacking forming process of structure engineering carrier frame

Abstract

The production process of human body tissue engineering carrier frame includes the following steps: firstly, preparing liquor containing tissue engineering carrier frame material and liquid containing growth factor at room temp., according to the user's requirements and previously designed and defined pathway extruding or jetting the above-mentioned two liquors respectively by means of different sprayers in the low-temp. environment of below zero deg.C, and making them form layer-by-layer stacked frozen porous tissue engineering carrier frame, then freeze-dried in freeze-drying machine, removing solvent so as to obtain the invented tissue engineering carrier frame. Its formation process and porosity structure of the frame, and the pore size and its through property can be well controlled,so that it is favorable for internal degradation of carrier frame in human body and nutrition and metabolism of regenerated tissue, and can well retain the bio-activity of biological material.

Description

technical field [0001] The invention belongs to the technical field of product forming technology, and in particular relates to a manufacturing forming technology and method of a human tissue engineering carrier frame. Background technique [0002] At present, the forming methods of carrier frameworks for tissue engineering in the world generally adopt foaming method, dissolved salt method, dipping method and thermally induced phase separation method, etc. These processes all follow a similar principle, that is to create pores through indirect means, such as adding foaming agents, sugar, inorganic salts, porous sponges and organic solvents as pore-forming agents. Direct relationship. [0003] For example, the typical process of the dipping method - the polyurethane sponge dipping method is to immerse the polyurethane sponge in the ceramic slurry, then dry it at 125°C for 1 hour, heat it to 500°C at a speed of 0.5°C / min, and then heat it at a rate of 1°C / min. The speed is h...

AI Technical Summary

Problems solved by technology

[0005] (1). It is difficult to form a controllable pore structure with a size range of 200μm-500μm and interpenetration

Since these processes are all indirect pore-forming (through foaming agents, inorganic salts, etc.), it is difficult to achieve direct and effective control of the pore structure of the carrier frame. The formed pore structure is random to a certain extent, and it is difficult to meet the requirements of tissue engineering. The morphological parameter requirements of the carrier framework - a controllable pore structure with a size range of 200μm-500μm and interpenetration;

[0006] (2). It is difficult to realize the formation of pore gradient structure and material gradient structure

This kind of carrier frame composed of uniform pore structure and materials is difficult to coordinate the contradiction between the degradation performance and mechanical strength of the carrier frame, and it is difficult to meet the complex performance requirements of the tissue engineering carrier frame;

[0007] (3). Difficult to realize customized carrier frame manufacturing

Due to the wide variety of tissue defects in different cases, it is necessary to design and manufacture the carrier frame for specific cases, which is difficult to achieve with traditional methods

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