Populus alba * pobulus davidiana dode 1333 tissue culture medium of pobulus davidiana dode improved variety
A technology of tissue culture medium and culture medium, which is applied in the field of plant tissue culture, can solve the problems of poor in vitro rapid propagation of regenerated tissues, and achieve good application prospects, normal leaf shape and color, and robust growth
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specific Embodiment approach 1
[0022] Specific embodiment one: the tissue culture medium of Populus zhongshan improved variety Yin×Shan 1333 of the present invention includes three kinds of culture medium: adventitious bud differentiation medium, subculture proliferation medium and adventitious bud rooting medium;
[0023] Wherein, the adventitious bud differentiation medium is composed of YS medium, 0.1mg / L-1.5mg / L 6-benzylaminopurine and 0mg / L-0.1mg / L kinetin;
[0024] The subculture growth medium is composed of YS medium, 0.1mg / L-1.0mg / L 6-benzylaminopurine and 0mg / L-0.05mg / L naphthaleneacetic acid;
[0025] The adventitious bud rooting medium is composed of YS medium and hormone A, and the hormone A is composed of 0.1mg / L-2.0mg / L indole butyric acid, 0.1mg / L-2.0mg / L indole One or more of acetic acid and 0.01mg / L-2.0mg / L naphthaleneacetic acid in any proportion;
[0026]Among them, the YS culture medium (YS culture medium) of Yang x Shan 1333 tissue basal medium (YS medium) is composed of macroelements,...
specific Embodiment approach 2
[0035] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the adventitious bud differentiation medium is composed of YS medium and 6-benzylaminopurine with a concentration of 1.0 mg / L and 0.1 mg / L kinetin composition. Others are the same as in the first embodiment.
[0036] In this embodiment, the callus cultured in the Adventitious Bud Differentiation Medium of Populus fine variety Yin×Shan 1333 can reach 88%, and the differentiation coefficient is 4.23; 1.5 to 4.7; Adventitious bud rooting medium culture of populus cultivar Yin×Shan 1333 can make the rooting rate reach more than 76.5%, rooting 5 to 11, the transplanting survival rate is also higher than 90.5%.
specific Embodiment approach 3
[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the adventitious bud differentiation medium is composed of YS medium and 6-benzylaminopurine with a concentration of 0.5 mg / L and 0.1 mg / L The kinetin composition of L. Others are the same as in the first or second embodiment.
[0038] In this embodiment, the callus cultured on the Adventitious Bud Differentiation Medium of Populus fine variety Yin×Shan 1333 can reach 78%, and the differentiation coefficient is 3.59; 1.5 to 4.7; Adventitious bud rooting medium culture of populus cultivar Yin×Shan 1333 can make the rooting rate reach more than 76.5%, rooting 5 to 11, the transplanting survival rate is also higher than 90.5%.
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