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Aspen silver × Shan 1333 tissue culture medium

A tissue culture medium and culture medium technology, applied in plant cells, horticulture, botany equipment and methods, etc., can solve the problems of poor in vitro rapid propagation effect of regenerated tissue, achieve good application prospects, normal leaf shape and leaf color, The effect of an accelerated process

Active Publication Date: 2015-12-02
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the existing culture medium has poor effect on the regeneration tissue of the hybrid progeny of Populus aspen and Populus alba Yin×Shan 1333 in vitro, and to provide tissue culture of the improved variety of Populus silver×Shan 1333 base

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0022] Specific embodiment one: the tissue culture medium of Populus zhongshan improved variety Yin×Shan 1333 of the present invention includes three kinds of culture medium: adventitious bud differentiation medium, subculture proliferation medium and adventitious bud rooting medium;

[0023] Wherein, the adventitious bud differentiation medium is composed of YS medium, 0.1mg / L-1.5mg / L 6-benzylaminopurine and 0mg / L-0.1mg / L kinetin;

[0024] The subculture growth medium is composed of YS medium, 0.1mg / L-1.0mg / L 6-benzylaminopurine and 0mg / L-0.05mg / L naphthaleneacetic acid;

[0025] The adventitious bud rooting medium is composed of YS medium and hormone A, and the hormone A is composed of 0.1mg / L-2.0mg / L indole butyric acid, 0.1mg / L-2.0mg / L indole One or more of acetic acid and 0.01mg / L-2.0mg / L naphthaleneacetic acid in any proportion;

[0026]Among them, the YS culture medium (YS culture medium) of Yang x Shan 1333 tissue basal medium (YS medium) is composed of macroelements,...

specific Embodiment approach 2

[0035] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the adventitious bud differentiation medium is composed of YS medium and 6-benzylaminopurine with a concentration of 1.0 mg / L and 0.1 mg / L kinetin composition. Others are the same as in the first embodiment.

[0036] In this embodiment, the callus cultured in the Adventitious Bud Differentiation Medium of Populus fine variety Yin×Shan 1333 can reach 88%, and the differentiation coefficient is 4.23; 1.5 to 4.7; Adventitious bud rooting medium culture of populus cultivar Yin×Shan 1333 can make the rooting rate reach more than 76.5%, rooting 5 to 11, the transplanting survival rate is also higher than 90.5%.

specific Embodiment approach 3

[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the adventitious bud differentiation medium is composed of YS medium and 6-benzylaminopurine with a concentration of 0.5 mg / L and 0.1 mg / L The kinetin composition of L. Others are the same as in the first or second embodiment.

[0038] In this embodiment, the callus cultured on the Adventitious Bud Differentiation Medium of Populus fine variety Yin×Shan 1333 can reach 78%, and the differentiation coefficient is 3.59; 1.5 to 4.7; Adventitious bud rooting medium culture of populus cultivar Yin×Shan 1333 can make the rooting rate reach more than 76.5%, rooting 5 to 11, the transplanting survival rate is also higher than 90.5%.

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Abstract

The invention relates to a populus alba * pobulus davidiana dode 1333 tissue culture medium of an pobulus davidiana dode improved variety, which is used for solving a problem that the existing culture medium is poor in in-vitro rapid propagation effect for populus alba* pobulus davidiana dode 1333 regeneration tissue of hybrid offspring pobulus davidiana dode improved variety of pobulus davidiana dode and populus alba. The populus alba* pobulus davidiana dode 1333 tissue culture medium of the pobulus davidiana dode improved variety comprises an adventitious bud differentiation culture medium, a subculture multiplication culture medium and an adventitious bud rooting culture medium, wherein the adventitious bud differentiation culture medium consists of a YS (Eosine Yellowish) culture medium, 6-benzyl amido purine and kinetin; the subculture multiplication culture medium consists of the YS culture medium, the 6-benzyl amido purine and naphthylacetic acid; the adventitious bud rooting culture medium is composed of the YS culture medium and hormone A, wherein the hormone A consists of one or several of indolebutyric acid, indoleacetic acid and naphthylacetic acid in proportion. The populus alba * pobulus davidiana dode 1333 tissue culture medium disclosed by the invention is applied to the plant tissue culture filed.

Description

technical field [0001] The invention relates to a tissue culture medium of populus fine variety Yin×Shan 1333, and relates to the field of plant tissue culture. Background technique [0002] Poplar is generally recognized as a high-quality fast-growing tree species for timber forests, shelter forests and landscaping in northern my country. However, among the more than 1,000 species selected in the past half century, there is no species suitable for afforestation of forest forests and mountain forests. And my country is the country that is famous in the world for mountainous area, and mountainous area accounts for more than 2 / 3rds of national land area, so it is very necessary to carry out popularization and transformation of new varieties of mountainous poplar. Aspen (Populus davidiana Dode), a native tree species in Northeast China, is widely distributed, has strong adaptability and excellent material quality, and is suitable for the construction of mountain timber forests ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 白卉王臣李春明邢亚娟王艳敏李海霞卢慧颖卓泳杉郭成博
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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