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Tissue culture method for improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333

A technology of tissue culture and improved seeds, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems such as the survival rate of rooted seedlings in tissue culture, low reproduction coefficient, and difficulty in tissue culture, etc., and achieve good application Foreground, the effect of high transplanting survival rate and high multiplication coefficient

Active Publication Date: 2015-03-18
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of difficult tissue culture, low reproduction coefficient, unhealthy rooted seedlings in tissue culture and low survival rate of transplanting of the existing Populus improved variety Yin×Shan 1333, and to provide the improved Populus improved variety Yin×Shan 1333 tissue culture method

Method used

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  • Tissue culture method for improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333
  • Tissue culture method for improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333
  • Tissue culture method for improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333

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specific Embodiment approach 1

[0025] Specific implementation mode one: the tissue culture method of the improved poplar variety Yin×Shan 1333 in the present embodiment is to be completed according to the following steps:

[0026] 1. Collection and pre-treatment of explants: Rinse the explants of the populus cultivar Yin×Shan 1333 under running water for 1-2 hours, then disinfect the surface with ethanol solution with a mass concentration of 70%-75% for 10s- 20s, then rinse 2-3 times with sterile water, then disinfect with 0.1% mercuric chloride for 2.0min-4.0min, rinse 3-5 times with sterile water, and finally dry the sterile water with sterile filter paper, and Processing the explants to obtain sterilized explants;

[0027] 2. Differentiation culture of explants: inoculate the sterilized explants obtained in step 1 into sterilized adventitious bud differentiation medium, and inoculate them at a temperature of 23°C to 26°C and a light time of 12h / d to 14h / d. Cultivate for 30 days to 45 days under the con...

specific Embodiment approach 2

[0042] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the explant of the populus fine variety Yin×Shan 1333 plant described in step 1 is: from a 25-year-old plant that is robust, has no wounds, and has no diseases and insect pests. The explants were taken from the plant of the improved poplar variety Yin×Shan 1333, and the stem segments with dormant buds of the improved poplar variety Yin×Shan 1333 plant were collected from January to February every year, and the leaves of the improved poplar variety Yin×Shan 1333 plant were collected 5 times a year , the stem section with axillary buds of the populus cultivar Yin×Shan 1333 plant is collected in May every year. When taking the explants of the plant of Populus silver × Shan 1333, it should be at noon or afternoon in fine weather. Others are the same as the first embodiment.

specific Embodiment approach 3

[0043] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the treatment of the explants in step 1 refers to peeling off the dormant buds from the stem, and peeling off the outer scales and outermost leaves , or cut the part of the leaf with petiole and without petiole into 0.8×0.8cm 2 ~1.2×1.2cm 2 square, or cut the stem segment with axillary buds into 0.5cm-1.5cm. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a tissue culture method for the improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333, which relates to a plant culture method. The objective of the invention is to overcome the problems of difficult tissue culture, a low propagation coefficient, non-robust rooted seedlings of tissue culture and a low survival rate of transplanting in conventional tissue culture of the improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333. The method comprises the following steps: 1, collection and preprocessing of an explant; 2, differentiation culture of the explant; 3, subculture multiplication of an adventitious bud; 4, rooting culture of the adventitious bud; 5, acclimatization of a regenerated tissue culture seedling; and 6, bottling-out and field planting of the tissue culture seedling of the improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333 so as to obtain a container seeding with a height of 40 to 50 cm of the tissue culture seedling of the improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333. The method provided by the invention is applied in the field of plant tissue culture.

Description

technical field [0001] The invention relates to a plant tissue culture method. Background technique [0002] Poplar is generally recognized as a high-quality fast-growing tree species for timber forests, shelter forests and landscaping in northern my country. However, among the more than 1,000 species selected in the past half century, there is no species suitable for afforestation of forest forests and mountain forests. And my country is the country that is famous in the world for mountainous area, and mountainous area accounts for more than 2 / 3rds of national land area, so it is very necessary to carry out popularization and transformation of new varieties of mountainous poplar. Aspen (Populus davidiana Dode), a native tree species in Northeast China, is widely distributed, has strong adaptability and excellent material quality, and is suitable for the construction of mountain timber forests in forest areas. However, its growth rate is slow, and the average annual DBH gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李春明白卉张妍妍李静王艳敏李海霞卢慧颖李京张建瑛
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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