95 results about "Lycorine" patented technology

The invention relates to a kind of effective constituent separation method in vegetation extract, especially the method of separating lycorine from vegetation extract containing amaryllidaceae alkaloid. The industrial art is as follows: make vegetation extract containing amaryllidaceae alkaloid have pretreatment; adsorb and elute with cation exchange resin; track and detect the elution flowing composition; make the flowing composition in lycorine have concentration and enrichment; after get virgin product of lycorine free alkali, make it have recrystallization with organic solvent; finally, the lycorine free alkali crystallization with about 99% purity can be acquired.

The invention discloses a technology method for distilling short-tube lycoris alkali from short-tube lycoris plants. It supplies the technology process and condition to distill short-tube lycoris alkali from the stem tuber of short-tube lycoris through cellulose enzyme enzymolysis. The purity of the product could reach 99%. And the method is easy to operate, and high efficient. The yield could reach 4.69 per ten thousand.

The invention discloses a process of extracting dihydrogalanthamine from Lycoris radiata plant, which in detail relates to a method for separating dihydrogalanthamine exquisite from alkaloid extracted from Lycoris radiata plant, and it is a recombined method of enzyme technology and chromatography. It comprises process and condition of extraction and separation of coarse lycorine, then extracting filtering liquid of coarse lycorine, condensing, preparing column material, loading column, eluting, condensing, recrystallizing and getting dihydrogalanthamine with purity of over 99%. The invention is different from method using organic solvent for dihydrogalanthamine extraction, and it can obtain coarse lycorine, coarse galantamin and dihydrogalanthamine with purity over 99%.

The invention belongs to the field of medicaments, discloses application of lycorine, lycorine salt, lycorine hydrate, lycorine optical isomer or lycorine prodrug in preparing medicaments for treating diseases caused by human enterovirus 71 type infection, preferably treating hand-foot-and-mouth disease, herpangina, viral meningitis, viral encephalitis, flaccid paralysis, pulmonary edema and vital myocarditis. In vitro and in vivo tests prove that the lycorine can inhibit replication and lesion of enterovirus (EV) 71 in cells, has excellent function of inhibiting the EV 71 virus, and has clinical application prospect.

The invention provides new application for a lycorine and bortezomib composition medicine. The lycorine and bortezomib composition is used for preparing a medicinal preparation for treating multiple myeloma. Compared with the fact that lycorine and bortezomib are used separately, combined use of lycorine and bortezomib can realize a better cell proliferation inhibition effect, and cell autophagy can be inhibited through combined use. The composition greatly improves sensibility of multiple myeloma to bortezomib and is applicable to drug-resistant multiple myeloma which is difficult to treat.

The present invention discloses the use of lycoramine in preparing medicine for resisting SARS virus, total lycoramine extract containing lycoramine and its preparation process, use in preparing medicine for resisting SARS virus and the composition therewith.

Fertilizer with insect expelling and antibacterial functions is prepared from main nutritious components in percentage by mass as follows: 15%-25% of nitrogen, 5%-15% of phosphorus and 10%-20% of potassium, and the fertilizer further comprises an insect expelling substance with the weight accounting for 3%-7% of the total weight of the fertilizer. The insect expelling substance is prepared from, in percentage by mass, 8%-12% of lycorine, 75%-85% of bamboo vinegar, 2%-7% of sodium lignin sulfonate, 2%-7% of tobacco powder and 3%-8% of polyethylene glycol through mixing. The fertilizer has the benefits as follows: active ingredients including lycorine and the tobacco powder with strong insect expelling activity are taken as the main insect expelling ingredients, the bamboo vinegar can inhibit growth of weeds when used for preparing the fertilizer, the dosage of pesticides can be reduced, the compound fertilizer has excellent insect expelling and hazard avoiding functions, sodium lignin sulfonate is used as a surfactant, all substances can be effectively and evenly mixed by use of polyethylene glycol, and the fertilizer efficiency and the insect expelling effect of the fertilizer are improved.

The invention discloses a nano-scale lycorine hydrochloride microcapsule emulsion and preparing method thereof. The nano-scale lycorine hydrochloride microcapsule emulsion comprises a water phase II and a lycorine hydrochloride nano microcapsule dispersed in the water phase II, the particle diameter range of the lycorine hydrochloride nano microcapsule is 5-100 nm; the lycorine hydrochloride nano microcapsule is composed of a water phase I and an oil phase, the water phase is packaged in the oil phase. The preparing method comprises (1) adding the water phase I into the oil phase to form pre-emulsifiable solution under 50000rmp-20000rmp high speed shearing condition; (2) mixing the pre-emulsifiable solution and the water phase II to obtain a premixing liquid under the stirring condition; (3) performing high-pressure homogeneity for the premixing liquid one-four times to obtain the nano-scale lycorine hydrochloride microcapsule emulsion under 100-200 MPa.

The invention discloses a breast cancer stem cell cryopreservation protection agent and a prepared cryopreservation protection kit. The cryopreservation protection agent consists of serum-free medium freeze-dried powder and fetal calf serum FBS, wherein the serum-free medium freeze-dried powder is prepared by the following steps: adding conventional growth factors, galanthamine and lycorine into a DMEM / F12 culture solution to obtain a culture solution of which the EGF concentration is 15-25g / L, the BSA concentration is 3-5g / L, the insulin concentration is 4-6mg / L, the galanthamine concentration is 50-100nmol / L and the lycorine concentration is 50-100nmol / L, wherein the conventional growth factors include recombinant human epidermal growth factor EGF, bovine serum albumin BSA, insulin and B27; storing in a shaking table for 20-28h at 37 DEG C in the presence of 5% CO2; and performing freeze drying. The cryopreservation protection agent disclosed by the invention can effectively reduce the damage of breast cancer stem cells caused by cryopreservation, the recovered cells have a high survival rate and strong tumorigenic ability and are not much different from those before cryopreservation, and thus the invention is remarkably superior to the prior art.

The invention provides a cultivation method of lycoris plants. The method comprises the following steps that LED light quality is used for treating lycoris plant seedlings, wherein the LED light quality is blue light (B) or red and blue compound light (RB). The content of alkaloid type ingredients of galanthamine, dihydrogalanthamine and lycorine in the lycoris plants cultivated by the method is obviously improved; the operation of the method is simple and convenient; the production period is short; the method is applicable to factory production; important significance is realized for meetingthe industrialized production of lycoris anticancer alkaloid.

The invention relates to a compound 1, 10-N-decylene lycorine dibromo salt showed by the structural formula (1), a pharmaceutical composition of the compound, application of the compound in the preparation of medicines for treatment of Alzheimer's diseases (senile dementia) and in the preparation of a dual inhibitor of acetylcholinesterase and butyrylcholinesterase, and preparation methods of the compound and the pharmaceutical composition.

The invention relates to application of a Lycorine compound in anti-tumor drugs, and also relates to application of its various optical isomers, pharmaceutically acceptable salts, solvates and prodrugs in preparation of anti-tumor drugs. The invention also relates to application of pharmaceutical compositions containing the Lycorine compound with a structure shown as formula (A) in preparation of anti-tumor drugs.

The invention provides a lycorine beta-aryl acrylate derivative as well as a preparation method and application thereof. The lycorine beta-aryl acrylate derivative has a structure as shown in a formula I, Ar is C6-12 aromatic hydrocarbon or C3-10 aromatic heterocycle, and heteroatoms in the aromatic heterocycle are selected from N, O and S; R is a substituent group on the Ar, is monosubstituted or polysubstituted, and the R is independently selected from hydrogen, halogen, halogenated C1-6 alkyl, halogenated C1-6 alkoxy, nitryl, hydroxyl, C1-6 alkyl, C1-6 alkoxy, C2-6 alkenyloxy, C2-6 alkenyl, C3-6 cycloalkyl and phenyl. The lycorine beta-aryl acrylate derivative disclosed by the invention has good anti-tumor activity and a relatively good medicinal prospect.

The invention discloses a lycoris plant insecticide which is prepared from the following materials in parts by weight: 30-50 parts of artemisia vulgaris, 10-20 parts of azedarach, 20-50 parts of moringa seeds, 20-35 parts of gleditsia sinensis, 5-10 parts of garlic juice, 5-10 parts of pomegranate barks, 200-500 parts of lycoris, 20-50 parts of tea bran, 30-50 parts of tobacco leaves and 10-20 parts of rose willows. The insecticide disclosed by the invention takes lycoris as a primary component of the insecticide. The lycoris contains lycorine. Through high pressure extraction, the insecticidal effective components in the lycoris are effectively extracted and have a remarkable killing ability on injurious insects. The lycoris is matched with the raw materials such as the tea bran, the moringa seeds and the rose willows, so that the effect of the insecticide disclosed by the invention can be remarkably improved. The insecticide is wide in insecticidal range and free of toxic and harmful effects.

The invention provides a method for rapidly culturing and producing galanthamine, lycorine and lycoramine by rapid propagation of bulblets of lycoris. The method comprises the following steps: taking a bulb of 'lycoris chinensis' as an explant, sterilizing, inoculating and culturing for a period of time, generating bulblets at a wound of a scale, and continuing carrying out multiplication culture on the bulblets; and drying tissues of the bulblets of the lycoris chinensis, grinding and ultrasonically extracting with methanol for three times to obtain a methanol solution containing the galanthamine, the lycorine and the lycoramine. The problems that resources of lycoris herbs are in shortage, and chemical synthesis of the galanthamine, the lycorine and the lycoramine is complicated are solved by a tissue culturing technology. According to a used basic medium, an SH medium is used as a base, and ingredients such as 6-benzylamino adenine, alpha-naphthylacetic acid and a yeast extract are used as auxiliary ingredients; and the method lays a foundation for industrial production of the galanthamine, the lycorine and the lycoramine and is environment-friendly, raw materials are abundant, and the supply gap of markets to the galanthamine, the lycorine and the lycoramine can be relieved well.

The present invention relates to anti-flavivirus compounds, including lycorine and derivatives thereof, and their use in treating a subject infected by a flavivirus. The present invention also relates to the use of the anti-flavivirus compounds for the prophylaxis of flavivirus infection. The present invention further relates to a method of suppressing viral RNA synthesis of a flavivirus. Also described is a method of preparing an anti-flavivirus compound for use in the treatment or prophylaxis of flavivirus infection.

The invention discloses a new application of lycorine serving as HMGB1 protein inhibitor. Lycorine comprises lycorine or hydrates thereof or salt capable of being accepted pharmaceutically. It is proved through a series of experiments that lycorine has the high inhibiting effect on HMGB1 protein and can serve as the HMGB1 protein inhibitor. Diseases such as multiple myeloma caused by HMGB1 protein expression abnormality can be treated by means of lowering the HMGB1 protein level, and lycorine is expected to be developed into novel anti-tumor medicine.

The invention relates to lycorine and derivative thereof which are represented by a structural formula I and II, and also relates to a veterinary drug which takes pharmaceutically acceptable salt of the lycorine and the derivative thereof as active constituents, a preparation method of compound (1-O-(thiophanate) methyl-2-O-acetyl lycorine (KY-91)), and applications of the compound in preparation of an inhibitor for porcine reproductive and respiratory syndrome and the preparation of the veterinary drug for the porcine reproductive and respiratory syndrome (blue-eared pig disease). A new psychotherapeutic drug can be provided for porcine reproductive and respiratory syndrome virus, and has the advantages of moderate dose, convenience in drug use, obvious curative effect, economy, suitability, low toxic and side effects.

The invention discloses a reagent and kit for enriching breast cancer stem cells from breast cancer cell strains. The reagent includes galantamine and lycorine as a screening agent. The breast cancer cell strains are MCF-7 breast cancer cell strains. The kit comprises a double-antibody, galantamine and lycorine as a screening agent, a screening-agent-dissolving auxiliary agent, sodium sulfite as an anti-oxidant, a recombinant human epidermal growth factor (EFG) which is a conventional growth factor, bovine serum albumin (BSA), insulin and B27. The provided reagent can enrich breast cancer stem cells from human breast cancer MCF-7 cell strains in a convenient, fast and efficient way, the cell spheroids are short in forming period, and the content of cancer cells and SP cells marked by CD44<+>CD24<- / low> molecules in microsphere cells is high. The developed kit can improve the efficiency for enriching cancer stem cells by science researchers.

The invention provides a yellow peach tree culturing fertilizer with insect-expelling anti-bacterial functions and a preparation method thereof. The yellow peach tree culturing fertilizer comprises the following components in parts by weight: 20-25 parts of dry animal waste, 20-22 parts of tobacco straws, 8-10 parts of bamboo vinegar, 6-9 parts of lycorine, 3-5 parts of monopotassium phosphate, 2-4 parts of bacterial manure and 3-5 parts of urea. The invention aims to solve the problems that the present yellow peach yield and quality cannot meet the practical requirement and the plant diseases and insect pests are serious under the condition of no pesticide application. The invention belongs to the field of yellow peach planting.

The invention discloses a method for simultaneously detecting the contents of three alkaloids in a lycoris plant, which is capable of quickly extracting and qualitatively and quantitatively analyzingalkaloid matters of galanthamine, lycoramine and lycorine in the lycoris plant. According to the method disclosed by the invention, gradient eluting is carried out by taking an LC (Liquid Chromatography)-MS (Mass Spectrometry) technology as the basis, analysis is carried out in an MRM (Multiple Reaction Monitoring) mode under positive ion scanning by connecting LC with tandem MS, the three alkaloid matters in the lycoris plant can be quickly extracted and accurately, qualitatively and quantitatively analyzed, the analysis time can be shortened to a large extent, the analysis accuracy is increased, a detection result can be obtained within a program time which is not more than 6.0 minutes, the retention time of the three alkaloids is within 3 minutes, the adding standard recovery rate is 97.2 to 100.2 percent, the RSD (Relative Standard Deviation) is 0.8 to 4.3 percent, and the analysis speed and the analysis accuracy are greatly increased; an effective and accurate analysis method is provided for researching an accumulation law and constructive metabolism regulation of the three alkaloids in the lycoris plant.

The invention discloses a compound microbial fertilizer. The compound microbial fertilizer comprises the following raw materials in parts by weight: 2-9 parts of a microbial rapid decomposition agent,30-46 parts of wheat straw, 22-35 parts of corn straw, 22-34 parts of cake meal, 20-34 parts of sodium hydroxide, 14-19 parts of fly ash, 25-32 parts of livestock and poultry manure, 12-21 parts of ground phosphate rock, 14-18 parts of potassium chloride, 10-25 parts of bamboo vinegar and 5-15 parts of lycorine. The compound microbial fertilizer can promote plant growth and inhibit weed growth, is quick in cellulose decomposition, and is suitable for being used as a substitute of an existing compound microbial fertilizer.

The invention relates to a method for extracting lycorine from Lycoris plants, which comprises the following steps: 1. extraction: weighing Lycoris plant powder which is subjected to drying, pulverization and screening, adding water with the pH value of 1.5-7.5 according to the material-liquid ratio (g / mL) of 1:(5-25), adding an enzyme which accounts for 1-5 wt% of the Lycoris plant powder, carrying out enzymolysis at 40-80 DEG C for 0.5-2.5 hours, centrifuging for 20 minutes, filtering, and taking the supernate to obtain a lycorine extracting solution; 2. purification: (1) carrying out solvent extraction; (2) adsorbing with a cation exchange resin; (3) carrying out C18 reversed-phase silica gel column chromatography; (4) carrying out normal-phase silica gel column chromatography; and (5) recrystallization: dissolving the dried substance III obtained in the step (4) in ethanol, recrystallizing to obtain a lycorine crude product, filtering, and carrying out vacuum drying at 50-60 DEG C to obtain the lycorine. The method is simple to operate, and has the advantages of low production cost, no environment pollution, short reaction time and higher extraction yield (30.46% higher than other methods). The purification technique has the advantages of environment friendliness and high purity (up to 99.3%).

The invention provides a method for preparing galanthamine and a water retaining gel from lycoris. The method comprises the following steps: refluxing dried lycoris by using a 70-90% alcoholic solution to extract lycorine, performing column separation to obtain galanthamine, washing lycoris residues by using an acrylic acid solution, and compounding with a catalyst to obtain the water retaining gel. According to the method, the starch in the lycoris is fully used for preparing water retaining gel, and the pollution of lycoris wastes to the environment is avoided. An acrylic acid aqueous solution is directly used for washing to dissolve out starch, so that operation steps are reduced, the time for treating the lycoris wastes is shortened, and the production cost of lycorine is reduced.

The invention discloses application of a Chinese herbal medicine micro-molecule monomer compound (lycorine) to preparation of potential drugs for treating prostate tumors and related diseases. The invention further discloses a prostate cancer resistance molecular mechanism of the Chinese herbal medicine micro-molecule monomer compound.

The invention discloses a kind of straw composite organic fertilizer and a preparation method thereof. The straw composite organic fertilizer contains the following components: 200-300 parts by weight of straws, 20-30 parts by weight of soybean curb residue, 10-20 parts by weight of vinasse, 5-8 parts by weight of phosphorite powder, 6-10 parts by weight of humic acid, 2-4 parts by weight of trace elements, 4-6 parts by weight of a composite microbiological inoculant, 1-3 parts by weight of mint powder, 0.8-1.5 parts by weight of lycorine, 3-5 parts by weight of pericarpium granati powder, 1-2 parts by weight of sodium potassium tartrate and 4-7 parts by weight of activated carbon. The straw composite organic fertilizer disclosed by the invention has the advantages that the straw is adopted as a main raw material, the sodium potassium tartrate and activated carbon are added, heavy metal elements in soil can be removed, and the aim of purifying the soil is achieved, so that healthy growth of crops is guaranteed; and the mint powder, lycorine and pericarpium granati powder are added, so that the fertilizer has functions of expelling parasites and avoiding harm, the soil has disease-and-insect-resistant capability, and yield of the crops is increased.

The invention provides a method for extracting lycorine. The method comprises the following steps: (1) soaking lycoris radiate bulb powder with water, then adding a compound enzyme for hydrolysis, carrying out filtering, and merging the filtrate; and (2), adding acidic clay into the filtrate obtained in the step (1), carrying out stirring, and the carrying out filtering; (3) adjusting the pH valueof the filtrate obtained in the step (2) by using a saturated sodium carbonate solution, carrying out filtering after standing, extracting the filter cake obtained by filtering, merging the filtrate,and concentrating the filtrate into paste to obtain a lycorine crude product; and (4) carrying out reflux and crystallization on the lycorine crude product obtained in the step (3) to obtain a lycorine pure product. The method disclosed by the invention has the advantages that the purity of the lycorine extracted from the plant lycoris radiate is greater than or equal to 99%, the product yield isincreased, the solvent loss is less than or equal to 2%, and the lycorine crude product which is high in purity and can be used for anti-tumor lycorine products is obtained, so that the yield is improved to the maximum extent, and a good technical support is created for large-scale production.

The invention belongs to the technical field of medicine, relates to amaryllidaceae alkaloids, and particularly provides a lycorine alkaline compound extracted from hybridized lycoris (a hybrid product of L. aurea and L. radiata). The compound has a protective effect on nerve cells, has prevention and treatment effects on Alzheimer's disease, and can be used for preparation of neuroprotective agents and drugs for preventing Alzheimer's disease.