Seedling growing method of bletilla striata

A Bletilla striata and seed technology, applied in the field of seedling raising, can solve the problems of increased seedling risk, low germination rate and high seedling cost, and achieve the effects of reduced seedling cost, short germination time and low seedling cost.

Active Publication Date: 2018-05-15
湘西自治州宏盛生态农业旅游开发有限公司
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  • Abstract
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Problems solved by technology

[0004] In the existing technology, seedlings raised by traditional tissue culture technology need to go through one sowing and two medium transfers, and the labor cost is very high; while the germination rate of direct seeding seedlings is too low, less than 5%, so the demand for seeds is particularly large

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  • Seedling growing method of bletilla striata

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Embodiment 1

[0035] 1. Seed germination:

[0036] 1) Capsule disinfection: first rinse the capsules with running water, rinse with 0.1% detergent solution, wash with distilled water, soak in 75% alcohol for 30 seconds, then soak in 0.1% mercury liter solution for 10 minutes, and finally rinse with sterile water 5 to 8 times , placed on a clean bench to dry.

[0037] 2) Preparation of germination medium: adopt semi-gel medium, formula: 2.0g / L high-strength agar (gel strength 1400g / cm 2 ), sucrose 30g / L, NAA 0.5g / L, banana 20g / L, 1 / 2MS.

[0038] 3) Sowing: Use tweezers to clamp the decontaminated fruit pods, cut open the fruit pods with a blade, and shake off the seeds on the surface of the germination medium. Be careful not to shake the medium, so as not to cause the seeds to sink due to the rupture of the medium. The culture conditions are: temperature 20~25℃,

[0039] 4) Inducing bulbs: at the beginning, the temperature is controlled at 20-25° C., the light intensity is 1500-2200 Lux, ...

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Abstract

The invention provides a seedling growing method of bletilla striata. The method comprises the following steps: the whole seedling growing process is divided into two stages, wherein in the first stage, seeds are uniformly scattered on the surface layer of a culture medium and germination cultivation is conducted in a tissue culture chamber; and after the seeds are germinated to form protocorms, second-stage cultivation is conducted, the protocorms are stripped from the culture medium and are put into water, the protocorms are scattered on a seedling bed after being dispersed, the protocorms are exposed on the substrate, nutritional liquid is sprayed on the seedling bed at fixed period, moisture preservation, temperature control and shading of the seedling bed are considered, direct sunlight is prevented, and water and fertilizer management as well as disease prevent and control management are conducted. The seedling growing method of the bletilla striata is high in germination efficiency, short in germination time, simple and convenient in management and low in seedling growing cost. Germination can be completed after 10 to 15 days and tissue culture chamber cultivation can be completely after 30 to 35 days. Inoculation on the culture medium is needed only for one time, the protocorms are scattered on the seedling growth substrate after being formed and seedling growing is conducted.

Description

[0001] basic field [0002] The invention belongs to the technical field of seedling cultivation, and in particular relates to a method for cultivating bletilla striata seedlings. Background technique [0003] Bletilla striata is a traditional Chinese medicinal material in my country, but its seeds have a low germination rate in nature due to the lack of endosperm, and now wild resources are decreasing. In order to meet people's demand for this medicinal material, people began to try artificial breeding and cultivation. Now the common Bletilla striata seedling methods are mainly divided into two categories: plant tissue culture rapid propagation and live seedling cultivation. [0004] In the existing technology, seedlings raised by traditional tissue culture technology need to go through one sowing and two medium transfers, and the labor cost is very high; while the germination rate of direct seeding seedlings is too low, less than 5%, so the demand for seeds is particularly l...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01G31/00A01H4/005
Inventor 贺爱国陈锦李向荣黄艳宁
Owner 湘西自治州宏盛生态农业旅游开发有限公司
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