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Preparation method for rapidly separating tremella fuciformis pure hyphae and tremella fuciformis mother strains

A technology of separation and purification, white fungus, applied in the field of preparation of Tremella female species, can solve problems such as unfavorable long-term preservation, weakened vitality, easy to turn yellow, etc., and achieves the effects of shortening preparation time, growing robustly, and avoiding damage

Active Publication Date: 2020-04-21
GUANGZHOU GENSOURCE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tremella pure mycelium is stored on agar medium, which tends to turn yellow, weaken its vitality, easily gelatinize to form small ear pieces, or transform into yeast-like conidia, which is not conducive to long-term storage

Method used

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  • Preparation method for rapidly separating tremella fuciformis pure hyphae and tremella fuciformis mother strains
  • Preparation method for rapidly separating tremella fuciformis pure hyphae and tremella fuciformis mother strains
  • Preparation method for rapidly separating tremella fuciformis pure hyphae and tremella fuciformis mother strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment is a preparation method for rapidly separating pure white fungus mycelium and white fungus female species, and its specific preparation steps are as follows:

[0025] (1) Separation of incense ash mycelium: On the mushroom bag of white fungus, dig a small piece of sawdust near the bottom part with marble-like markings, and insert it into the test tube of potato dextrose agar medium. This part contains a lot of incense ash mycelium, after inoculation, it is placed in an environment of 23-25°C, and after 3-5 days, white and thick mycelium will grow, then turn yellow-green, and then turn black. Pick up the black part with an inoculation needle and insert it into a new test tube. Transfer several times like this, promptly get pure incense ash mycelia.

[0026] (2) Preparation of sawdust culture medium containing ash fungus culture: inoculate the separated and purified mycelium of incense ash on the sterilized bottled sawdust medium, and cultivate it in an e...

Embodiment 2

[0030] Present embodiment is the growth situation of white fungus pure mycelia on agar medium ( image 3 ).

[0031]The white fungus fruiting body that meets the ear ear standard in Example 1 is used as the separation material, and a small ear piece of about 1 square centimeter is cut, washed 2-3 times with sterile water, and placed in 75% alcohol or 0.1% mercuric chloride Soak in the solution for two minutes to kill bacteria on the surface of the ear piece. Immediately put the small ear piece soaked in the fungicide into sterile water, rinse 2-3 times, then blot the water with sterile gauze, and inoculate it into the sterilized agar medium.

Embodiment 3

[0033] This embodiment is the growth situation of white fungus pure mycelium on wood chip medium.

[0034] The white fungus fruiting body that meets the ear ear standard in Example 1 is used as the separation material, and a small ear piece of about 1 square centimeter is cut, washed 2-3 times with sterile water, and placed in 75% alcohol or 0.1% mercuric chloride Soak in the solution for two minutes to kill bacteria on the surface of the ear piece. Immediately put the small ear piece soaked in the fungicide into sterile water, rinse 2-3 times, then blot the water with sterile gauze, and inoculate it into the sterilized sawdust medium.

[0035] Embodiment 1,2,3 result contrast is as follows:

[0036] The Growth of Tremella Fungus Pure Mycelia on Different Media

[0037]

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Abstract

The invention discloses a preparation method of a tremella fuciformis mother strain. According to the method, a wood chip culture medium containing the Xianghuijun strain culture is prepared by separating the Xianghuijun strain hyphae, and then the pure tremella fuciformis hyphae obtained through separation and the Xianghuijun strain hyphae are subjected to cross culture on the wood chip culture medium containing the Xianghuijun strain culture to prepare the tremella fuciformis mother strain. Compared with a traditional method, the separated pure tremella fuciformis mycelia have the advantagesof being short in germination time, robust in growth, pure white, dense and the like; separation of tremella fuciformis pure hyphae and preparation of tremella fuciformis mother strains are completedin the same bottle of wood chip culture medium, operation is easy and convenient, damage to the tremella fuciformis pure hyphae caused by multiple times of transfer and infectious microbe pollution possibly occurring in the transfer process are avoided, meanwhile, the preparation time of the tremella fuciformis mother strains is shortened, and the preparation cost of the tremella fuciformis mother strains is effectively reduced.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for preparing white fungus female species. Background technique [0002] Tremella is the fruiting body of fungi of the genus Tremella, Tremella, Tremella, Tremellaceae, and Tremella. It is also known as Tremella, Tremella, and Tremella. Tremella is generally in the shape of a chrysanthemum or a cockscomb, soft and white, translucent and elastic. Tremella, as a traditional edible fungus in my country, has always been a favorite food of the people. It is usually used as the main ingredient for cooking various dishes, and the active ingredient tremella polysaccharide contained in it has special health care functions. The biggest difference from common edible fungus strains is that Tremella fungus has two kinds of strains, that is, regardless of the preparation of mother species, original species or cultivated species, each test tube, each strain bottle, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 涂桂洪邓惠平谭志康潘忠林陈品健
Owner GUANGZHOU GENSOURCE BIO TECH CO LTD
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