Method for promoting germination of siraitia grosvenorii seeds

A technology of Luo Han Guo and seeds, applied in the directions of seed immunization, seed and rhizome treatment, application, etc., can solve the problems of complex operation, low germination rate, low germination force, etc., and achieve the effect of simple operation, short germination time and high germination rate

Inactive Publication Date: 2018-02-16
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method for promoting the germination of Luo Han Guo seeds is complex in operation, high in cost, relatively low in germination ability, and low in germination rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Harvest the fruits at the mature stage, peel off the fruit sacs, wash off the pulp, remove the husks and take out the seeds after drying in the shade, pick out 200 Luo Han Guo seeds with no damage, full grains, uniform texture, and no disease and insect eggs, and randomly divide them into 2 groups of 100 pieces each.

[0031] 2. Put the first group of Luo Han Guo seeds into a bleaching powder solution with a mass concentration of 0.4%, and place them for 10 minutes. Take out the seeds and put them in a 9% mass concentration of trisodium phosphate solution, and leave them for 10 minutes. Take out the seeds and wash them with water 3 times. , to obtain seed I; put the second group of Luo Han Guo seeds into distilled water for 20 minutes, and take out to obtain seed II.

[0032] 3. Put the seed I into a mixed solution containing kinetin and gibberellin, wherein the concentration of kinetin in the mixed solution is 5mg / L, and the concentration of gibberellin in the mixed...

Embodiment 2

[0034] 1. Harvest the fruits at the mature stage, peel off the fruit sacs, wash off the pulp, remove the husks and take out the seeds after drying in the shade, pick out 200 Luo Han Guo seeds with no damage, full grains, uniform texture, and no disease and insect eggs, and randomly divide them into 2 groups of 100 pieces each.

[0035] 2. Put the first group of Luo Han Guo seeds in a 70% ethanol solution for 20 minutes, take out the seeds and put them in a 10% mass concentration of trisodium phosphate solution for 15 minutes, take out the seeds and wash them with water 4 times , to obtain seeds I; put the second group of Luo Han Guo seeds into distilled water for 35 minutes, and take them out to obtain seeds II.

[0036] 3. Put the seed I into a mixed solution containing kinetin and gibberellin, wherein the concentration of kinetin in the mixed solution is 10 mg / L, and the concentration of gibberellin in the mixed solution is 180 mg / L, The seeds II were placed in distilled wa...

Embodiment 3

[0038] 1. Harvest the fruits at the mature stage, peel off the fruit sacs, wash off the pulp, remove the husks and take out the seeds after drying in the shade, pick out 200 Luo Han Guo seeds with no damage, full grains, uniform texture, and no disease and insect eggs, and randomly divide them into 2 groups of 100 pieces each.

[0039] 2. Put the first group of Luo Han Guo seeds in an ethanol solution with a mass concentration of 85%, and place them for 30 minutes. Take out the seeds and put them in a trisodium phosphate solution with a mass concentration of 11%, and place them for 20 minutes. Take out the seeds and wash them with water 4 times. , to obtain seed I; put the second group of Luo Han Guo seeds into distilled water for 50 minutes, and take out to obtain seed II.

[0040] 3. Put the seed I into a mixed solution containing kinetin and gibberellin, wherein the concentration of kinetin in the mixed solution is 30mg / L, and the concentration of gibberellin in the mixed sol...

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PUM

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Abstract

The invention discloses a method for promoting germination of siraitia grosvenorii seeds, wherein the method comprises the following steps of 1, taking the siraitia grosvenorii seeds; putting the siraitia grosvenorii seeds into disinfectant; placing the seeds for 10 to 30 minutes; taking out the seeds to be put into a trisodium phosphate solution with the mass concentration being 9 to 11 percent;placing the seeds for 10 to 20 minutes; taking out the seeds; cleaning the seeds by water to obtain seeds I; 2, placing the seeds I in a mixed solution containing kinetin and gibberellin for 12 to 36hto obtain seeds II; culturing the obtained seeds II for 10 to 30 days for germination, wherein the concentration of the kinetin in the mixed solution is 5 to 30mg / L, and the concentration of the gibberellin in the mixed solution is 100 to 300mg / L. The method solves the problems of weak germination force and low germination rate of the siraitia grosvenorii seeds, and achieves the effects that thegermination time is short, the budding is regular, and the germination rate reaches 90 percent or higher.

Description

technical field [0001] The invention relates to the technical field of plant seed germination, in particular to a method for promoting the germination of Luo Han Guo seeds Background technique [0002] Monk fruit (Siraitia grosvenorii) is a perennial vine of the genus Cucurbitaceae, and it is a unique medicinal and sweet material plant in China. Food is also Chinese medicine in the list of medicines. Luohanguo is a famous authentic medicinal material in Guangxi. The production of Luohanguo in Guangxi accounts for more than 90% of the world. It has become an important characteristic industry in northern Guangxi, and has won the National Geographical Indication Protection Product. [0003] Luo Han Guo is the main active ingredient of Luo Han Guo for antitussive. At the same time, mogroside is a natural sweetener with low calorie and high sweetness, which is 300 to 400 times sweeter than sucrose. patients, etc. to eat. In recent years, more and more enterprises in my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/08A01C1/00
CPCA01C1/08A01C1/00
Inventor 潘丽梅马小军白隆华韦树根冯世鑫黄芩芬覃芳
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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