Method for culturing of edible fungi

A cultivation method and technology for edible fungi, which are applied in the directions of botanical equipment and methods, application, and horticulture, can solve the problems of high energy consumption, high bacterial infection rate, small inoculation amount, etc., and achieve sufficient oxygen supply and uniform distribution of bacterial species. , the effect of short germinative time

Inactive Publication Date: 2010-07-21
ZHEJIANG UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for cultivating edible fungi, which solves the problems of small inoculation amount, high bacterial infection rate and high energy consumption in the existing method

Method used

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  • Method for culturing of edible fungi
  • Method for culturing of edible fungi
  • Method for culturing of edible fungi

Examples

Experimental program
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Embodiment 1

[0029] Embodiment 1: mushroom cultivation method

[0030] (1) Prepare a liquid primary seed medium. The formula is (w / v): 3% soluble starch, 2% sucrose, 0.1% yeast extract, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, prepared with water, pH 6.0. After preparation, it is divided into 500ml Erlenmeyer flasks, each bottle is 100ml, sterilized at 0.1MPa for 30 minutes, and cooled for later use.

[0031] (2) Preparation of liquid primary species. Pick the mushroom block (together with part of the culture medium) into the sterilized and cooled primary seed culture medium, and cultivate it in a shaker flask at 180 rpm for 6 days at 25°C.

[0032] (3) Preparation of cultivar medium. The formula is (w / v): 20% potato extract (20 grams of potatoes are boiled and filtered to make 100 milliliters of potato juice), 2% sucrose, 0.2% corn flour, 0.1% fine wood chips, and the pH is natural. Put 60 liters of culture medium in a 100 liter fermenter, and after sterilizing the...

Embodiment 2

[0038] Embodiment 2: Grifola frondosa cultivation method.

[0039] (1) Preparation of liquid primary seed medium. The formula is (w / v): 20% of potato extract (20 grams of peeled potatoes are boiled in water for 20 minutes and then filtered, and the filtrate is made into 100 mL of first-class medium), 2% of sucrose, 0.25% of potassium dihydrogen phosphate, sulfuric acid Magnesium 0.05%, Peptone 0.05%, pH Natural. Divide into 500ml Erlenmeyer flasks, 100ml per bottle. Sterilize at 0.1MPa for 30 minutes, cool down for use.

[0040] (2) Preparation of liquid primary species. Pick the frondosa frondosa mother species bacterial block (together with part of the culture medium) into the sterilized and cooled first-grade species culture medium, and cultivate it in a shaker flask at 160 rpm for 5 days at 27°C.

[0041] (3) Preparation of cultivar medium. The formula is (w / v): 20% of potato extract, 2% of sucrose, 0.5% of corn flour, 0.5% of peptone, and natural pH. Pack 45 liters ...

Embodiment 3

[0047] Embodiment 3: black fungus cultivation method.

[0048] (1) Preparation of liquid primary seed medium. The formula is (w / v): 20% of potato extract (20 grams of peeled potatoes are boiled in water for 20 minutes and then filtered, and the filtrate is made into 100 mL of first-class medium), 2% of sucrose, 0.25% of potassium dihydrogen phosphate, sulfuric acid Magnesium 0.05%, pH natural. Divide into 500ml Erlenmeyer flasks, 100ml per bottle. Sterilize at 0.1MPa for 30 minutes, cool down for use.

[0049] (2) Preparation of liquid primary species. Pick the black fungus mother species bacterium block (together with part of the culture medium) into the sterilized and cooled primary species culture medium, and culture it in a shaker flask at 180 rpm at 23°C for 7 days.

[0050] (3) Preparation of cultivar medium. The formula is (w / v): 20% of potato extract, 2% of sucrose, 0.5% of corn flour, 0.5% of bean cake powder, and 0.1% of fine sawdust. Put 30 liters of culture m...

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Abstract

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for cultivating edible fungi. Background technique [0002] There are two main methods for the production of edible fungi, liquid culture method and solid state culture method. The liquid culture method is a method in which bacteria are inoculated into a liquid medium and cultured in a fermenter. The liquid culture method is convenient for mechanized operation, suitable for factory production, and has low labor intensity; but the requirements for raw materials are relatively high, and only mycelium (balls) are harvested, and the discharge of a large amount of fermentation waste liquid will pollute the environment. The solid-state cultivation method is to inoculate the strains into a solid medium and cultivate them in a mushroom house (shed); this method can use cheap raw materials to harvest fruit bodies with high value, and there is no discharge of wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 吴根福李卫旗
Owner ZHEJIANG UNIV
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