Method for preparing phalaenopsis plant tissue medium

A technology of medium preparation and medium formulation, applied in the field of agricultural biology, can solve the problems of low reproduction coefficient of Phalaenopsis ramets, slow propagation speed of hybrid breeding, difficult germination of hybrid seeds, etc., and achieve high uniformity of seedlings and high rooting rate Effect

Inactive Publication Date: 2013-01-02
SHANDONG JINQIU SEED IND
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Problems solved by technology

[0003] The object of the present invention is to provide a kind of Phalaenopsis plant tissue culture medium preparation method, to solve the extremely low reproduction coefficient of Phalaenopsis ramets, slow hybrid br

Method used

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[0014] Taking the preparation of 1 liter differentiation medium and 1 liter rooting medium as an example, the raw material formula ratio is: seed germination medium: seed germination medium formula: 10 times MS mother liquor 0.1 liter, 6-benzylaminopurine 5 mg, 1 -Naphthalene acetic acid 0.2 mg, activated carbon 1000 mg, sucrose 20000 mg, agar powder 5000 mg; differentiation medium formula: 10 times MS mother liquor 0.0333 liters, 6-benzylaminopurine 3 mg, 1-naphthalene acetic acid 0.2 mg, activated carbon 1000 mg, Banana juice 100000 mg, sucrose 30000 mg, agar powder 5000 mg; strong seedling medium formula: 10 times MS mother liquor 0.1 liter, 6-benzylaminopurine 1.5 mg, indole-3-butyric acid 1.5 mg, Huabao No. 1 500 Mg, Huabao No. 2 1000 mg, activated charcoal 2000 mg, sucrose 200000 mg, agar powder 5000 mg / L; rooting medium formula: 10 times MS mother liquor 0.1 L, 1-naphthylacetic acid 0.5 mg, banana juice 100000 mg, activated carbon 2000 Mg, 20000 mg of sucrose, 5000 mg of...

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Abstract

The invention provides a method for preparing a phalaenopsis plant tissue medium and relates to a plant tissue culture technology. The method for preparing the monstera deliciosa plant tissue medium adopts a tissue culture technology, the formula of the medium consists of a seed germination medium, a differential medium, a strong seedling medium and a rooting medium, and the four media are respectively prepared and applied. The formula of the seed germination medium comprises minimal medium Murashige and Skoog (MS), 6-benzyl aminopurine, 1-naphthaleneacetic acid, activated carbon, cane sugar and agar powder; the formula of the differential medium comprises minimal medium 1/3MS, the 6-benzyl aminopurine, the 1-naphthaleneacetic acid, the activated carbon, banana juice, the cane sugar and the agar powder; the formula of the strong seedling medium comprises the minimal medium MS, the 6-benzyl aminopurine, benzaole-3-butyric acid, hyponex No.1, hyponex No.2, the activated carbon, the cane sugar and the agar powder; the formula of the rooting medium comprises the minimal medium MS, the 1-naphthaleneacetic acid, the banana juice, the activated carbon, the can sugar and the agar powder; and the required medium products are respectively prepared and obtained according to the formulas, the phalaenopsis plant tissue culturing and high power breeding are realized.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology and relates to a plant tissue culture technology. Background technique [0002] Phalaenopsis is one of the most widely cultivated and popular species in Orchidaceae. Due to the large flowers, long flowering period, bright colors, various colors, and beautiful flower shapes, they are deeply loved by people and have a large market demand. However, the traditional branch reproduction coefficient of Phalaenopsis is extremely low, and the propagation speed of hybrid breeding is slow. It is not easy to germinate, and it is difficult to meet market demand. Many scholars have done a lot of research on the tissue culture of Phalaenopsis, but there are many hybrid varieties of Phalaenopsis, and there is a lack of a medium with wide adaptability and satisfying the industrial production of multiple varieties. Contents of the invention [0003] The object of the present invention is to provide a k...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张友秋赵学燕王双双王道帅
Owner SHANDONG JINQIU SEED IND
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