91 results about "Indole-3-butyric acid" patented technology

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings / transplants of plants.

The invention discloses a tissue culture method for lilium tenuifolium, which comprises: sterilizing stripped lilium tenuifolium bulb serving as an explant from an intermediate scale part; and inoculating the stripped lilium tenuifolium bulb into a Murashige and Skoog (MS) medium, a first-generation induction medium consisting of 6-benzyladenine (BA) at a concentration of 1.0mg / L, nicotinic acid amide (NAA) at a concentration of 0.1mg / L and vitamin C (Vc) at a concentration of 5mg / L, a sub-generation propagation medium consisting of MS, 6-BA at a concentration of 0.2mg / L and NAA at a concentration of 0.01mg / L, a rooting medium consisting of 1 / 2of MS, and indole-3-butyric acid (IBA) at a concentration of 0.5 mg / L and bulbing culture medium consisting of 1 / 2of MS, IBA at a concentration of 0.5 mg / L and 2 percent of white sugar in turn for culture under the culture conditions including a culture temperature of 25+ / -1 DEG C, day light and 4 hours of night light supply per day. When the culture method is used, tissue culture lilium tenuifolium seedlings propagated indoor in large scale can be obtained in a short time period, the productivity is high and the potential ecological benefitand social benefit can be created.

The invention relates to a method for artificially inducing resistance to jujube witches broom, and the method comprises the following steps: longitudinally cutting a cut at the lower end of the stem section of a cultured plantlet in a healthy group of a susceptible variety, wherein the stem section is utilized as a scion; removing leaves and axillary bus from the stem section of the cultured plantlet in a group infected with the jujube witches broom, and cutting one surface of the upper end of the stem section into a diagonal plane, wherein the stem section is utilized as a rootstock; then inserting the scion, aligning the rootstock with a scion formation layer, and using an aluminum foil to bind a grafting port, thus acquiring a micro-grafted plantlet; inserting into an MS (Murashige and Skoog) culture medium without any plant growth regulator for culture; adding 1mg / ml of tetracycline to kill pathogens in the micro-grafted plantlet after graft survival and afference of the pathogens into the scion, cutting the scion, transferring into the MS culture medium which is additionally added with 1.0mg / mL of BA (6-benzyl adenine) and 0.5mg / mL of IBA (indole-3-butyric acid) for rejuvenation, and further grafting the scion after the rejuvenation onto the rootstock of the cultured plantlet in the group with the jujube witches broom; and continuously performing the induction containingmicro-grafting inoculation, processing with the tetracycline, rejuvenation and micro-grafting inoculation above three times, thus obtaining a plant with resistance to the jujube witches broom. By utilizing the method, the plant with the resistance to the jujube witches broom can be rapidly induced and obtained. By adopting the method, the resistance to the jujube witches broom of jujube witches broom-sensitive good jujube tree varieties can be rapidly improved in an orientation way, thus the method provided by the invention has very broad application prospects.