Tissue culture method for lilium tenuifolium

A technology for tissue culture of Lilium chinensis, applied in the field of plant biology, can solve the problems of less research on tissue culture of wild lily, and achieve the effect of increasing the induction differentiation rate, increasing the induction success rate, and reducing the pollution rate
CN102204512BActive Publication Date: 2013-04-17INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
Publication Date
2013-04-17

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Abstract

The invention discloses a tissue culture method for lilium tenuifolium, which comprises: sterilizing stripped lilium tenuifolium bulb serving as an explant from an intermediate scale part; and inoculating the stripped lilium tenuifolium bulb into a Murashige and Skoog (MS) medium, a first-generation induction medium consisting of 6-benzyladenine (BA) at a concentration of 1.0mg / L, nicotinic acid amide (NAA) at a concentration of 0.1mg / L and vitamin C (Vc) at a concentration of 5mg / L, a sub-generation propagation medium consisting of MS, 6-BA at a concentration of 0.2mg / L and NAA at a concentration of 0.01mg / L, a rooting medium consisting of 1 / 2of MS, and indole-3-butyric acid (IBA) at a concentration of 0.5 mg / L and bulbing culture medium consisting of 1 / 2of MS, IBA at a concentration of 0.5 mg / L and 2 percent of white sugar in turn for culture under the culture conditions including a culture temperature of 25+ / -1 DEG C, day light and 4 hours of night light supply per day. When the culture method is used, tissue culture lilium tenuifolium seedlings propagated indoor in large scale can be obtained in a short time period, the productivity is high and the potential ecological benefitand social benefit can be created.
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Description

technical field

[0001] The invention belongs to the field of plant biotechnology, in particular, relates to the establishment of a tissue culture and regeneration system of Lily lily, including the selection and sterilization of explants, the screening of medium in different periods, and the influence of culture conditions on the growth of cultures. Developmental effects, rapid propagation techniques and the establishment of regeneration systems, etc. Background technique

[0002] Lilium pumilum, also known as Shandan flower, is a perennial bulbous flower of the genus Lilium in the family Liliaceae. The culm is 30-40 cm high, with leaves on the stem, slender and slender, narrow and long like pine leaves. The flowers droop, open in late spring and early summer, the petals roll outwards, the color is bright red, usually without spots, sometimes there are a few spots near the base, shiny, fragrant, very beautiful. The flowering period is June-August, and the fruiting period i...

Claims

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