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Tissue culture method for lilium tenuifolium

A technology for tissue culture of Lilium chinensis, applied in the field of plant biology, can solve the problems of less research on tissue culture of wild lily, and achieve the effect of increasing the induction differentiation rate, increasing the induction success rate, and reducing the pollution rate

Active Publication Date: 2013-04-17
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been more than hundreds of lily species and varieties that have been successfully tissue-cultured at home and abroad, but most of them are reported as cultivars for fresh-cut flower production, and there are few studies on tissue culture of Chinese wild lilies.

Method used

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  • Tissue culture method for lilium tenuifolium
  • Tissue culture method for lilium tenuifolium
  • Tissue culture method for lilium tenuifolium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1. The selection of Lilium fine-leaved explants and the influence of operation method on the induction of adventitious buds

[0031] 1. Materials and methods: The explants are the bulbs of Lilium tenifolia, and the bulbs are collected from the surrounding mountains of Hohhot, Inner Mongolia. The collection time is when budding or just sprouting in spring, and the explants are taken from the middle scales of Lilium tenifolia. Then sterilize the explants. The treatment steps are: wash the scales slightly to remove the soil → put them in a sterile container on the ultra-clean bench → add 2-3 drops of "Tween-20" to the detergent solution per liter Soak for 60 seconds → soak in 75% alcohol for 30 seconds → treat with 0.15% mercuric acid solution for 20 minutes → rinse with sterile water for 3-4 times → blot dry on sterile filter paper → directly access the pre-prepared in a good medium. It should be noted that the material should not be too much each time, and th...

Embodiment 2

[0045] Example 2. Screening of the optimal medium for each stage of Lilium microphylla tissue culture

[0046] 1. The effect of hormone concentration and its ratio on the induction of adventitious buds in Lilium microphylla

[0047] After the scales were inserted into the induction medium, about 40% of the scales turned purple-red after 3-7 days, then turned green and gradually formed small protrusions after 6-30 days, and about 90% of the scales turned green after 15-45 days The scales turn green. Adventitious buds were formed at the base, middle or upper part of the scales 30-55 days after turning green, but adventitious buds were often formed at the base, and almost no adventitious buds were formed at the upper part.

[0048] The scales are placed on MS medium containing different plant hormones and ratios. The culture environment is: culture temperature 25±1°C, light intensity 2500Lx, in addition to natural light during the day, supplementary light for 4 hours / day at nigh...

Embodiment 3

[0061] Embodiment 3. hardened seedling transplanting and greenhouse cultivation after Lilium fine-leaved rooting

[0062] The test-tube seedlings that grow robustly in the rooting medium and whose roots grow to 1-2cm are transplanted for hardening, firstly for 2 days of closed bottle hardening, then for 2-3 days of open bottle hardening, and then transferred to the greenhouse Plant. Carefully pour out the test-tube seedlings when transplanting, rinse the residual medium on the roots with clean water, dip the roots with 50ppm IBA for 8 minutes, and then plant them into the four substrates designed in advance. The results showed that vermiculite + sheep manure + diammonium phosphate had the best effect as the cultivation substrate, which was very suitable for the growth of seedling root system, and the transplanting survival rate could reach more than 80%. When the tissue-cultured seedlings grow to 3-4 true leaves, they can be moved into a false plant nursery for artificial pla...

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Abstract

The invention discloses a tissue culture method for lilium tenuifolium, which comprises: sterilizing stripped lilium tenuifolium bulb serving as an explant from an intermediate scale part; and inoculating the stripped lilium tenuifolium bulb into a Murashige and Skoog (MS) medium, a first-generation induction medium consisting of 6-benzyladenine (BA) at a concentration of 1.0mg / L, nicotinic acid amide (NAA) at a concentration of 0.1mg / L and vitamin C (Vc) at a concentration of 5mg / L, a sub-generation propagation medium consisting of MS, 6-BA at a concentration of 0.2mg / L and NAA at a concentration of 0.01mg / L, a rooting medium consisting of 1 / 2of MS, and indole-3-butyric acid (IBA) at a concentration of 0.5 mg / L and bulbing culture medium consisting of 1 / 2of MS, IBA at a concentration of 0.5 mg / L and 2 percent of white sugar in turn for culture under the culture conditions including a culture temperature of 25+ / -1 DEG C, day light and 4 hours of night light supply per day. When the culture method is used, tissue culture lilium tenuifolium seedlings propagated indoor in large scale can be obtained in a short time period, the productivity is high and the potential ecological benefitand social benefit can be created.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular, relates to the establishment of a tissue culture and regeneration system of Lily lily, including the selection and sterilization of explants, the screening of medium in different periods, and the influence of culture conditions on the growth of cultures. Developmental effects, rapid propagation techniques and the establishment of regeneration systems, etc. Background technique [0002] Lilium pumilum, also known as Shandan flower, is a perennial bulbous flower of the genus Lilium in the family Liliaceae. The culm is 30-40 cm high, with leaves on the stem, slender and slender, narrow and long like pine leaves. The flowers droop, open in late spring and early summer, the petals roll outwards, the color is bright red, usually without spots, sometimes there are a few spots near the base, shiny, fragrant, very beautiful. The flowering period is June-August, and the fruiting period i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王召明梁德霖姚裕琪
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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