Culturing medium antiseptic for Anoectochilus roxburghii (Wall.) Lindl tissue culture, and its preparation method

A technology of culture medium and antibacterial agent, which is applied in the field of preparation of antibacterial agent of culture medium in the process of tissue culture of A. effect, promote healthy growth, and increase the content of active ingredients

Active Publication Date: 2014-07-16
漳州美利德生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of tissue culture, due to the influence of many factors such as environment, culture medium and artificial technology, the pollution rate of seedling bottles is high, thereby increasing the cost of tissue culture
The current solution is to add bactericides to the culture medium, such as penicillin or chloramphenicol. Antibiotics such as tetracycline, or even pesticides such as carbendazim, although it has a certain bactericidal effect, it is difficult to control pesticide residues. The addition of antibiotics and pesticides and fungicides also affects the growth of clematis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation steps of the culture medium antibacterial agent of every 1000 milliliters of Clematis clematis tissue culture of the present invention are:

[0020] Step 1, preparation of MS medium mother solution:

[0021] Mother liquor A: 18.5 grams of magnesium sulfate, 82.5 grams of ammonium nitrate, 95.0 grams of potassium nitrate, add water to make up to 1000 milliliters, add 1 liter of culture medium to take 20 milliliters of antibacterial dosage

[0022] Mother solution B: Add 22.0 grams of calcium chloride to 500 milliliters of water, add 1 liter of culture medium antibacterial agent to take 10 milliliters.

[0023] Mother solution C: Add 8.5 grams of potassium dihydrogen phosphate to 500 ml of water, mix with 1 liter of culture medium and take 10 ml of antibacterial dose

[0024] Mother liquor D: 3.73 grams of disodium edetate and 2.78 grams of ferrous sulfate, add water to 1000 milliliters, add 1 liter of medium antibacterial agent and 10 milliliters.

[00...

Embodiment 2

[0035] The preparation steps of the culture medium antibacterial agent of every 1000 milliliters of Clematis clematis tissue culture of the present invention are:

[0036] Step 1, preparation of MS medium mother solution:

[0037] ①Mother solution A: 18.5 grams of magnesium sulfate, 82.5 grams of ammonium nitrate, 95.0 grams of potassium nitrate, add water to make up to 1000 milliliters, mix with 1 liter of culture medium and take 22.5 milliliters.

[0038] ②Mother solution B: Add 22.0 grams of calcium chloride to 500 milliliters of water, mix with 1 liter of medium to take? 12.5 ml.

[0039] ③Mother solution C: Add 8.5 grams of potassium dihydrogen phosphate to 500 ml of water, and mix with 1 liter of medium to measure? 12.5 ml.

[0040] ④Mother solution D: 3.73 g disodium edetate and 2.78 g ferrous sulfate, add water to 1000 ml, add 12.5 ml to 1 liter medium.

[0041] ⑤ Mother liquor E: 620 mg of boric acid, 2230 mg of manganese sulfate, 860 mg of zinc sulfate, 2.5 mg of...

Embodiment 3

[0051] The preparation steps of the culture medium antibacterial agent of every 1000 milliliters of Clematis clematis tissue culture of the present invention are:

[0052] Step 1, preparation of MS medium mother solution:

[0053] ①Mother solution A: 18.5 grams of magnesium sulfate, 82.5 grams of ammonium nitrate, 95.0 grams of potassium nitrate, add water to make the volume to 1000 milliliters, mix with 1 liter of culture medium and take 25 milliliters.

[0054] ②Mother solution B: add 22.0 g of calcium chloride to 500 ml of water, and prepare 1 liter of culture medium to take 15 ml.

[0055] ③Mother solution C: Add 8.5 grams of potassium dihydrogen phosphate to 500 milliliters of water, mix with 1 liter of culture medium and take 15 milliliters

[0056] ④Mother solution D: 3.73 g of disodium edetate and 2.78 g of ferrous sulfate, add water to 1000 ml, add 1 liter of culture medium and add 15 ml.

[0057] ⑤ Mother liquor E: 620 mg of boric acid, 2230 mg of manganese sulfate...

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Abstract

A culturing medium antiseptic for Anoectochilus roxburghii (Wall.) Lindl tissue culture comprises an MS culturing medium mother liquor or a B5 culturing medium mother liquor, and each 1000ml of the culturing medium antiseptic also comprises 1-5g of chitosan, 1-4g of tea polyphenol, 80-120g of mashed banana, 15-30g of sucrose, 2-4g of peptone, 0.2-0.5mg of indole 3-butyric acid potassium, 0.2-0.6mg of pimacol and 0.5-1mg of 6-benzylaminopurine. The pH value of the culturing medium antiseptic is adjusted to 5.4-5.7 by hydrochloric acid or sodium hydroxide. The culturing medium antiseptic adopts chitosan and tea polyphenol, and the chitosan and tea polyphenol have good bacteriostasis and sterilization effects, are health preserving competitive products beneficial for the health, can effectively reduce the pesticide residues, and can promote the healthy growth of Anoectochilus roxburghii (Wall.) Lindl. The mashed banana and peptone are rich in organic potassium and organic nitrogen, can effectively meet the nutrient demands of Anoectochilus roxburghii (Wall.) Lindl, and can also improve the content of effective components in Anoectochilus roxburghii (Wall.) Lindl in order to ensure the drug effect.

Description

technical field [0001] The invention relates to the biotechnology of tissue culture of clematis, in particular to the preparation technology of medium antibacterial agent in the process of tissue culture of clematis. Background technique [0002] Clematis clematis is a kind of precious Chinese medicinal material, known as the king of medicine. Because there are very few natural wild plants, the prospect of tissue culture planting is good. Most of the existing tissue culture medium of A. roxburghii adopts MS medium or B5 medium as the mother solution, and adds peptone, agar and the like. In the process of tissue culture, due to the influence of many factors such as environment, culture medium and artificial technology, the pollution rate of seedling bottles is high, thereby increasing the cost of tissue culture. The current solution is to add bactericides to the culture medium, such as penicillin or chloramphenicol. Antibiotics such as tetracycline, or even pesticides such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N61/00A01P1/00A01P3/00C05G3/00A01N43/16
Inventor 黄阿贤
Owner 漳州美利德生物工程有限公司
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