Culturing medium antiseptic for Anoectochilus roxburghii (Wall.) Lindl tissue culture, and its preparation method

Abstract

A culturing medium antiseptic for Anoectochilus roxburghii (Wall.) Lindl tissue culture comprises an MS culturing medium mother liquor or a B5 culturing medium mother liquor, and each 1000ml of the culturing medium antiseptic also comprises 1-5g of chitosan, 1-4g of tea polyphenol, 80-120g of mashed banana, 15-30g of sucrose, 2-4g of peptone, 0.2-0.5mg of indole 3-butyric acid potassium, 0.2-0.6mg of pimacol and 0.5-1mg of 6-benzylaminopurine. The pH value of the culturing medium antiseptic is adjusted to 5.4-5.7 by hydrochloric acid or sodium hydroxide. The culturing medium antiseptic adopts chitosan and tea polyphenol, and the chitosan and tea polyphenol have good bacteriostasis and sterilization effects, are health preserving competitive products beneficial for the health, can effectively reduce the pesticide residues, and can promote the healthy growth of Anoectochilus roxburghii (Wall.) Lindl. The mashed banana and peptone are rich in organic potassium and organic nitrogen, can effectively meet the nutrient demands of Anoectochilus roxburghii (Wall.) Lindl, and can also improve the content of effective components in Anoectochilus roxburghii (Wall.) Lindl in order to ensure the drug effect.

Description

technical field [0001] The invention relates to the biotechnology of tissue culture of clematis, in particular to the preparation technology of medium antibacterial agent in the process of tissue culture of clematis. Background technique [0002] Clematis clematis is a kind of precious Chinese medicinal material, known as the king of medicine. Because there are very few natural wild plants, the prospect of tissue culture planting is good. Most of the existing tissue culture medium of A. roxburghii adopts MS medium or B5 medium as the mother solution, and adds peptone, agar and the like. In the process of tissue culture, due to the influence of many factors such as environment, culture medium and artificial technology, the pollution rate of seedling bottles is high, thereby increasing the cost of tissue culture. The current solution is to add bactericides to the culture medium, such as penicillin or chloramphenicol. Antibiotics such as tetracycline, or even pesticides such a...

AI Technical Summary

Problems solved by technology

In the process of tissue culture, due to the influence of many factors such as environment, culture medium and artificial technology, the pollution rate of seedling bottles is high, thereby increasing the cost of tissue culture

The current solution is to add bactericides to the culture medium, such as penicillin or chloramphenicol. Antibiotics such as tetracycline, or even pesticides such as carbendazim, although it has a certain bactericidal effect, it is difficult to control pesticide residues. The addition of antibiotics and pesticides and fungicides also affects the growth of clematis