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Method for simultaneously determining multiple plant growth regulators in exported vegetables

A technology for growth regulators and assay methods, applied in the field of chemical analysis and detection, can solve problems such as potential safety hazards, immune dysfunction, endocrine system disorders, etc.

Inactive Publication Date: 2015-08-05
INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the residual plant growth regulators in vegetables also bring safety hazards to people's health, such as reproductive and endocrine system disorders, immune dysfunction, cancer and other potential diseases

Method used

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  • Method for simultaneously determining multiple plant growth regulators in exported vegetables
  • Method for simultaneously determining multiple plant growth regulators in exported vegetables
  • Method for simultaneously determining multiple plant growth regulators in exported vegetables

Examples

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Embodiment Construction

[0053] The specific implementation of the present invention will be further described below in conjunction with the examples. The following examples are used to illustrate the present invention, but are not intended to limit the protection scope of the present invention.

[0054] 1) extract

[0055] Use a homogenizer to make vegetable homogenate from the original vegetables, weigh 10.00g vegetable homogenate (accurate to 0.01g) into a 50mL centrifuge tube, add 20mL acetonitrile solution containing 1% (v / v) acetic acid, shake horizontally for 10min , then add 5.00g (accurate to 0.01g) of sodium chloride, vortex again for 1min, offline at 5000r / min for 3min, pipette 10mL of supernatant I into a 15mL centrifuge tube for purification.

[0056] 2) Purify the sample

[0057] Weigh 100mg of octadecylsilane bonded silica gel (ODS, 60μm) and 500mg of anhydrous magnesium sulfate in 10mL of supernatant Ⅰ, vortex and mix for 2min, 5000r / min offline for 3min, absorb supernatant Ⅱ into 25...

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Abstract

The invention discloses a method for simultaneously determining multiple plant growth regulators in exported vegetables. The method comprises the following steps: extraction, purification, concentration, ultrahigh-performance liquid chromatography-triple quadrupole / composite linear ion hydrazine mass spectrometer (UHPLC-QTRAP) determination and the like. The method can simultaneously determine the 17 plant growth regulators, including gibberellin, indolyl-3-butyric acid, p-chlorophenoxyacetic acid, abamectin, diethyl aminoethyl hexanoate, atrazine, simazine, 6-benzyl aminopurine, 2,4-dichlorphenoxyacetic acid, forchlorfenuron, mepiquat chloride, chlormequat chloride, paclobutrazol, uniconazole, abscisic acid, thidiazuron and p-fluorophenoxyacetic acid. The method disclosed by the invention has the advantages of simple steps, short time consumption, high accuracy and high precision.

Description

technical field [0001] The invention belongs to the field of chemical analysis and detection, and in particular relates to a simultaneous determination method for 17 kinds of plant growth regulators in export vegetables. Background technique [0002] Vegetables are not only the main supply source of various vitamins, potassium, magnesium, calcium, phosphorus, iron and other minerals and dietary fiber, but also an important source of phytochemical components such as carotenoids, plant polyphenols and other antioxidants. Antioxidant defense system and nutritional repair genes, correcting genetic weaknesses play an important role. In order to change the inherent mode of vegetable growth and development through chemical regulation, effectively improve vegetable production conditions, and improve vegetable yield and quality, plant growth regulators have been widely used in vegetable production. In recent years, the wide application of plant growth regulators on vegetables has br...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 曹淑瑞李贤良唐柏彬郗存显周雪张雷
Owner INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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