Method for artificially inducing resistance to jujube witches broom

Abstract

The invention relates to a method for artificially inducing resistance to jujube witches broom, and the method comprises the following steps: longitudinally cutting a cut at the lower end of the stem section of a cultured plantlet in a healthy group of a susceptible variety, wherein the stem section is utilized as a scion; removing leaves and axillary bus from the stem section of the cultured plantlet in a group infected with the jujube witches broom, and cutting one surface of the upper end of the stem section into a diagonal plane, wherein the stem section is utilized as a rootstock; then inserting the scion, aligning the rootstock with a scion formation layer, and using an aluminum foil to bind a grafting port, thus acquiring a micro-grafted plantlet; inserting into an MS (Murashige and Skoog) culture medium without any plant growth regulator for culture; adding 1mg/ml of tetracycline to kill pathogens in the micro-grafted plantlet after graft survival and afference of the pathogens into the scion, cutting the scion, transferring into the MS culture medium which is additionally added with 1.0mg/mL of BA (6-benzyl adenine) and 0.5mg/mL of IBA (indole-3-butyric acid) for rejuvenation, and further grafting the scion after the rejuvenation onto the rootstock of the cultured plantlet in the group with the jujube witches broom; and continuously performing the induction containing micro-grafting inoculation, processing with the tetracycline, rejuvenation and micro-grafting inoculation above three times, thus obtaining a plant with resistance to the jujube witches broom. By utilizing the method, the plant with the resistance to the jujube witches broom can be rapidly induced and obtained. By adopting the method, the resistance to the jujube witches broom of jujube witches broom-sensitive good jujube tree varieties can be rapidly improved in an orientation way, thus the method provided by the invention has very broad application prospects.

Description

technical field [0001] The invention relates to a method for artificially inducing resistance to jujube madness. Background technique [0002] Jujube witches'broom disease, caused by phytoplasma, is the most common and most harmful fatal infectious disease in jujube production. The jujube trees infected with jujube mad disease will die within 3-5 years for the light ones, and die after 1-2 years for the severe ones. Jujube madness is a disease of the vascular system, and its control is very difficult. Breeding excellent disease-resistant varieties is the fundamental way to solve the problem of jujube madness. So far, the phytoplasma of jujube madness cannot be artificially cultured in vitro, and the artificially cultivated phytoplasma cannot be used for routine pathogen inoculation. However, grafting and inoculation in the field is greatly restricted by natural factors such as season, climate and environment, and the conditions are difficult to control, the workload is lar...

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