Tissue culture method for lilium tenuifolium

A technology for tissue culture of lily lily, which is applied in the field of plant biology, can solve the problems of less research on tissue culture of wild lily, and achieve the effects of increasing the induction differentiation rate, reducing the pollution rate, and reducing the washing and soaking time
CN102204512AActive Publication Date: 2011-10-05INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
Publication Date
2011-10-05

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Abstract

The invention discloses a tissue culture method for lilium tenuifolium, which comprises: sterilizing stripped lilium tenuifolium bulb serving as an explant from an intermediate scale part; and inoculating the stripped lilium tenuifolium bulb into a Murashige and Skoog (MS) medium, a first-generation induction medium consisting of 6-benzyladenine (BA) at a concentration of 1.0mg / L, nicotinic acid amide (NAA) at a concentration of 0.1mg / L and vitamin C (Vc) at a concentration of 5mg / L, a sub-generation propagation medium consisting of MS, 6-BA at a concentration of 0.2mg / L and NAA at a concentration of 0.01mg / L, a rooting medium consisting of 1 / 2of MS, and indole-3-butyric acid (IBA) at a concentration of 0.5 mg / L and bulbing culture medium consisting of 1 / 2of MS, IBA at a concentration of 0.5 mg / L and 2 percent of white sugar in turn for culture under the culture conditions including a culture temperature of 25+ / -1 DEG C, day light and 4 hours of night light supply per day. When the culture method is used, tissue culture lilium tenuifolium seedlings propagated indoor in large scale can be obtained in a short time period, the productivity is high and the potential ecological benefitand social benefit can be created.
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Description

Technical field

[0001] The present invention belongs to the field of plant biotechnology. Specifically, it relates to the establishment of a tissue culture and regeneration system of Lilium tenuifolia, including the selection and sterilization of explants, the selection of media in different periods, and the influence of culture conditions on the growth of cultures. The influence of development, rapid reproduction technology and the establishment of regeneration system. Background technique

[0002] Lilium pumilum, also known as Shandan, is a perennial bulbous flower in the lily family. The stalk is 30-40 cm high, with leaves growing on the stem, slender and slender, as long as pine leaves. The flowers are drooping and open in late spring and early summer. The petals are rolled outwards, bright red in color, usually without spots. Sometimes there are a few spots near the base, shiny, with a delicate fragrance, and very beautiful. Flowering period from June to August, fruiting p...

Claims

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