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Tissue culture method for lilium tenuifolium

A technology for tissue culture of lily lily, which is applied in the field of plant biology, can solve the problems of less research on tissue culture of wild lily, and achieve the effects of increasing the induction differentiation rate, reducing the pollution rate, and reducing the washing and soaking time

Active Publication Date: 2011-10-05
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been more than hundreds of lily species and varieties that have been successfully tissue-cultured at home and abroad, but most of them are reported as cultivars for fresh-cut flower production, and there are few studies on tissue culture of Chinese wild lilies.

Method used

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  • Tissue culture method for lilium tenuifolium
  • Tissue culture method for lilium tenuifolium
  • Tissue culture method for lilium tenuifolium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. The influence of selection and operation methods of Lilium tenuifolia explants on adventitious bud induction

[0031] 1. Materials and methods: The explants are bulbs of lily lily. The bulbs are collected from the mountainous area around Hohhot, Inner Mongolia. The collection time is about to bud or just emerge in spring. The explants are taken from the middle scales of lily. Afterwards, the explants are sterilized. The processing steps are as follows: the scales are slightly rinsed to remove the dirt → put in a sterile container on the ultra-clean table → add 2-3 drops of "Tween-20" detergent solution per liter Soak for 60 seconds → transfer to 75% alcohol and soak for 30 seconds → then treat with 0.15% mercury solution for 20 minutes → rinse with sterile water 3-4 times → absorb water on sterile filter paper → directly connect to pre-prepared In a good medium. It should be noted that the material should not be too much each time, and the whole process should ...

Embodiment 2

[0045] Example 2. Selection of the best medium for each stage of tissue culture of Lilium tenuiflora

[0046] 1. The effect of hormone concentration and its ratio on the induction of adventitious buds in Lilium tenuiflora scales

[0047] After the scales are connected to the induction medium, about 40% of the scales turn into purple-red after 3-7 days, and then turn to green after 6-30 days and gradually form small protrusions. After 15-45 days, about 90% The scales turn green. Adventitious buds are formed at the base, middle or upper part of the scales 30-55 days after turning green, but adventitious buds are formed at the base more often, and there are almost no adventitious buds on the upper part.

[0048] The scales are connected to MS medium containing different plant hormones and proportions. The culture environment is: culture temperature 25±1°C, light intensity 2500Lx, in addition to natural light during the day, supplement light at night for 4 hours / day. On the 40th day, t...

Embodiment 3

[0061] Example 3. Seedling transplanting and greenhouse cultivation of Lily lily after rooting

[0062] The test-tube seedlings that grow robustly in the rooting medium and have a root length of 1-2 cm are transplanted. First, close the bottle for 2 days, then open the bottle for 2-3 days, and then transfer to the greenhouse. Planted. When transplanting, carefully pour out the test tube seedlings, rinse the root residual medium with clean water, soak the roots with 50ppm IBA for 8 minutes, and then plant them into the 4 pre-designed substrates. The results show that vermiculite + sheep manure + diammonium phosphate has the best effect as a cultivation substrate, which is very suitable for the growth of seedling roots, and the survival rate of transplanting can reach more than 80%. When the tissue cultured seedlings grow to 3-4 true leaves, they can be moved to a fake planting nursery for fake planting or directly planted. During this period, some diluted compound fertilizer wate...

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Abstract

The invention discloses a tissue culture method for lilium tenuifolium, which comprises: sterilizing stripped lilium tenuifolium bulb serving as an explant from an intermediate scale part; and inoculating the stripped lilium tenuifolium bulb into a Murashige and Skoog (MS) medium, a first-generation induction medium consisting of 6-benzyladenine (BA) at a concentration of 1.0mg / L, nicotinic acid amide (NAA) at a concentration of 0.1mg / L and vitamin C (Vc) at a concentration of 5mg / L, a sub-generation propagation medium consisting of MS, 6-BA at a concentration of 0.2mg / L and NAA at a concentration of 0.01mg / L, a rooting medium consisting of 1 / 2of MS, and indole-3-butyric acid (IBA) at a concentration of 0.5 mg / L and bulbing culture medium consisting of 1 / 2of MS, IBA at a concentration of 0.5 mg / L and 2 percent of white sugar in turn for culture under the culture conditions including a culture temperature of 25+ / -1 DEG C, day light and 4 hours of night light supply per day. When the culture method is used, tissue culture lilium tenuifolium seedlings propagated indoor in large scale can be obtained in a short time period, the productivity is high and the potential ecological benefitand social benefit can be created.

Description

Technical field [0001] The present invention belongs to the field of plant biotechnology. Specifically, it relates to the establishment of a tissue culture and regeneration system of Lilium tenuifolia, including the selection and sterilization of explants, the selection of media in different periods, and the influence of culture conditions on the growth of cultures. The influence of development, rapid reproduction technology and the establishment of regeneration system. Background technique [0002] Lilium pumilum, also known as Shandan, is a perennial bulbous flower in the lily family. The stalk is 30-40 cm high, with leaves growing on the stem, slender and slender, as long as pine leaves. The flowers are drooping and open in late spring and early summer. The petals are rolled outwards, bright red in color, usually without spots. Sometimes there are a few spots near the base, shiny, with a delicate fragrance, and very beautiful. Flowering period from June to August, fruiting p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王召明梁德霖姚裕琪
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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