Preparation method of apple dwarfing rootstock B9 plant tissue culture medium

Abstract

The invention discloses a preparation method of an apple dwarfing rootstock B9 plant tissue culture medium. A formula and proportions of a proliferation culture medium are as follows: a basic culturemedium MS, 0.5 to 1.0mg / L of 6-benzylamino adenine, 1.5 to 2.0mg / L of indole-3-butyric acid, 0.1mg / L of folic acid, 20 to 40g / L of sucrose and 3.5 to 4.5g / L of agar powder; a formula and proportions of a rooting culture medium are as follows: a basic culture medium 1 / 2 MS, 1.5 to 2.0mg / L of indole-3-butyric acid, 1.0 to 2.0mg / L of 1-naphthylacetic acid, 0.1mg / L of folic acid, 20 to 40g / L of the sucrose and 3.5 to 4.5g / L of the agar powder. According to the formula of the proliferation culture medium, the culture period can be greatly shortened and is nearly not changed; produced seedlings havea high uniformity degree; the formula of the rooting culture medium has a high rooting rate; after the rooting culture medium is inoculated for 18 days, the rooting rate is 85 to 90 percent; the quantity of roots of each seedling is 3 to 6; especially, the roots grow from basic parts of the seedlings and calluses are not formed between the roots and the seedlings; normal roots grow and puffed roots do not grow; the domestication survival rate can be greatly improved through the two characteristics so that the cost of tissue culture seedlings is reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for configuring a plant tissue culture medium. Background technique [0002] At present, the propagation method of apple dwarf rootstock B9 is mainly tissue culture and rapid propagation. However, in some tissue culture rooms, due to the inappropriate formulation of the proliferation medium and rooting medium, the differentiation factor is not high, only about 2 times, so the propagation speed is slow; The callus grows first, and then takes root from the callus, so that the upper and lower parts of the seedlings cannot conduct nutrients smoothly; some roots are a kind of puffed root, which cannot fully exert the absorption capacity of the root. This situation has greatly reduced the survival rate of tissue cultured seedlings domestication, thereby causing the cost of apple dwarf rootstock B9 tissue cultured seedlings to increase, no matter from quality, pri...

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Problems solved by technology

[0003] The object of the present invention is exactly in order to solve above-mentioned problem, provides a kind of plant tissue culture medium configuration method, to solve existing culture medium formula unreasonable, bottle seedling differentiation multiple is not high, propagation speed is slow; Low survival rate, increased seedling production costs and other issues; at the same time solve the problem that apple rootstocks are needed in some cold regions