Preparation method of apple dwarfing rootstock B9 plant tissue culture medium
A technology for plant tissue and dwarf rootstock, applied in the field of plant tissue culture, can solve the problems of low differentiation multiple of bottle seedlings, slow reproduction speed, poor rooting quality, etc. The effect of cycle shortening
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[0009] In order to make it easy to understand the technical means, creative features, goals and effects achieved by the present invention, the present invention will be further explained in conjunction with specific embodiments below.
[0010] An apple dwarf rootstock B9 plant tissue culture medium configuration method. The plant tissue culture medium comprises: a proliferation medium and a rooting medium; the formula and ratio of the proliferation medium are: basic medium MS, 6-benzylamino adenine 0.5-1.0mg / L, indole-3-butyric acid 1.5-2.0mg / L, folic acid 0.1mg / L, sucrose 20-40g / L, agar powder 3.5-4.5g / L; rooting medium formula ratio: Basic medium 1 / 2MS, indole-3-butyric acid 1.5-2.0mg / L, 1-naphthylacetic acid 1.0-2.0mg / L, folic acid 0.1mg / L, sucrose 20-40g / L, agar powder 3.5-4.5 g / L.
[0011] Take the apple dwarf rootstock B9 plant tissue culture with 20L multiplication medium and 20L rooting medium as an example:
[0012] Proliferation medium formula: basic medium 20 times MS, 6...
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