31results about How to "Improve domestication survival rate" patented technology

The invention relates to a wild pampus argenteus seedling acclimatizing method. The wild pampus argenteus seedling acclimatizing method includes feeding by stages. By means of feeding live baits and mixed feed, eating habits and digestive system of wild pampus argenteus can be changed step by step, batches of juvenile pampus argenteus are prevented from dying from flatulence caused by dyspepsia caused by bait change. The wild pampus argenteus seedling acclimatizing method has the advantages that survival rate and growing speed of artificial cultured pampus argenteus changed from the wild pampus argenteus can be increased effectively.

The invention belongs to the technical field of rare and peculiar fish farming and particularly relates to a feed training method of wild rhinogobio ventralis. The feed training method includes a hanger phase, an artificial inducing phase and a complete mixed-feed feeding phase; the artificial inducing phase includes two stages, one is a complete live-food feeding stage, and the other is an artificial mixed-feed inducing stage. With the method, the technical problem of difficult feed training of the wild rhinogobio ventralis under the condition of artificial breeding is successfully solved, the technical base of large-scale cultivation of parent fish of the rhinogobio ventralis is established, and large-scale propagation and large-scale releasing of the rhinogobio ventralis are made possible.

The invention discloses a liquid rapid-propagation method for rhizoma bletillae seeds. The method comprises the following steps: (1) explant selection and disinfection; (2) seed germination and young-seedling culture; (3) adult-seedling culture; and (4) seedling hardening and transplantation. The step of seed germination and young-seedling culture concretely comprises the sub-step of culturing the rhizoma bletillae seeds in a liquid medium, wherein culture conditions are as follows: temperature is 26+/-2 DEG C; illumination intensity is 3000 to 3500 Lx; illumination time is 14 to 16 hours/day; and culture time is 45 to 60 days. The liquid rapid-propagation method for the rhizoma bletillae seeds, provided by the invention, can realize industrialization of rhizoma bletillae seedling propagation and obtains rhizoma bletillae seedlings meeting the needs of large-scale production in a short time; meanwhile, the method also has the characteristics of low cost, neat seedling growing, high seedling-hardening domestication survival rate, etc.

The invention provides a method for d domesticating wild Leptobotia elongata under artificial cultivation conditions until the gonadal maturation of the Leptobotia elongata. The method includes: building an ecology-simulated pond to simulate the habitat of the wild Leptobotia elongata; subjecting the collected Leptobotia elongata to soaking and sterilizing, and performing isolated temporary cultivation; domesticating the Leptobotia elongata after the temporary cultivation in the ecology-simulated pond; feeding the Leptobotia elongata with prepared parent Leptobotia elongata feed; strengtheningbefore reproduction, to be more specific, three months before the reproduction of the Leptobotia elongata, injecting carp hypophysis cerebri grinding liquid once each month; two months before the reproduction, increasing the flow speed of the ecology-simulated pond, and increasing the water temperature to 25-26 DEG C to stimulate the gonadal maturation of the parent Leptobotia elongata. The method has the advantages that the habitat, water flow and food of the wild Leptobotia elongata are simulated, the nutrition proportion of the parent Leptobotia elongata feed is regulated, the strengthening before reproduction is performed, gonadal maturation rate can reach above 50%, and spawning induction rate during large-scale artificial reproduction is increased.

The invention relates to the field of medicinal plant tissue culture, in particular to a tissue culture and rapid propagation method of a medicinal plant stemona japonica, which comprises the following steps: 1) explant disinfection: selecting a stem section with buds of the stemona japonica, cleaning, and disinfecting by using 75% alcohol, a saturated bleaching powder solution, a 8% hydrogen peroxide solution and 0.025% mercury bichloride in sequence; 2) cluster bud induction: inoculating the disinfected explants into an induction culture medium for culture; 3) proliferation of cluster buds: inoculating the induced cluster buds into a proliferation culture medium according to polarity for culture; and 4) rooting of the cluster buds: cutting the proliferated cluster buds into single buds, and inoculating the single buds to a rooting culture medium according to polarity for culture. According to the method for tissue culture and rapid propagation of the radix stemonae, the disinfection contamination rate, the death rate, the proliferation withering and yellowing rate and the rooting withering and yellowing rate of the radix stemonae are far lower than those of an existing disclosed technical scheme, the 50-day induction rate, the 35-day proliferation coefficient, the 30-day rooting rate and the 50-day domestication survival rate are all higher than those of the prior art, and rapid propagation of the radix stemonae can be achieved.

The invention discloses a salmon seawater acclimation method. According to the method, the acclimation transition stage of salmon from freshwater to seawater is standardized, an existing acclimation method for 0-degree freshwater to 30-degree seawater step by step is changed, fingerlings of the specification being 400 g or larger are selected, the technical route for beginning acclimation from 15-degree saline water is adopted, the good adaptability of salmon can be achieved only within two weeks, the survival rate can reach 99% or above, the acclimation period is effectively shortened, and the cultivation efficiency is improved.

The invention discloses a two-step rooting method of a globe artichoke variety LORCA. The method comprises the following steps of 1 rooting culture medium preparing, 2 tissue culture seedling culture condition setting, 3 rooting culturing, 4 seedling hardening and 5 transplanting. The method has the advantages that globe artichoke tissue culture seedlings are rooted through the two-step rooting method, root system induction and growth are separated, and culture medium formulas used in all the steps are all initiated in globe artichoke tissue culture. By using white kraft paper, the humidity in a bottle is greatly reduced, and a vitrification phenomenon is effectively constrained. The kraft paper is very good in breathability, is mostly applied to bagging breeding of crops such as rice and rapes and can effectively prevent outside mould and the like from entering bags, and therefore bottle seedlings cannot be contaminated. By increasing the dosage of agar powder and conducting shading treatment, the vitrification rate can be effectively decreased, and the rooting rate can be effectively increased; the rooting rate is increased to 86% from 20%, and the bottle seedling domestication survival rate is increased to 92% from 65%.

The invention relates to the field of medicinal plant tissue culture and traditional Chinese medicinal materials, in particular to a culture medium for improving the domestication survival rate of euphorbia hirsuta tissue culture seedlings and a domestication method of the euphorbia hirsuta tissue culture seedlings. The culture method comprises the steps of explant disinfection, cluster bud induction, cluster bud proliferation, cluster bud rooting and rooting seedling domestication. According to the method, during rooting, low-concentration 2, 4-D and IBA are matched, rooting of euphorbia pekinensis can be effectively promoted, the rooting rate can be close to 100%, meanwhile, 2.0-2.4 mu g/L of silver nitrate and 0.2-0.3 mg/L of MET are added into a rooting bottle culture medium, the vitrification phenomenon of rooted seedlings can be completely inhibited, and the domestication survival rate can be increased to a great extent.

The invention provides a taming method of wild hucho bleekeri living bodies. The method comprises the steps of manufacturing a taming fishpond containing an oxygen increasing mouth, throwing schizothorax taliensis wild adults to conduct taming, and conducting feeding with cold water fishes or schizothorax taliensis offspring seeds and with an artificial nutrition feed additive, and comprises the specific steps of adopting local stones to build the taming fishpond of 4 m in length, 2 m in width and 1.5 m in depth, wherein the inner wall of the fishpond is troweled with cement, stored water is 0.8-1.2 m, and the fishpond is provided with a main water source, a subsidiary water source and an oxygen increasing head; putting the rescued hucho bleekeri wild living bodies into the taming fishpond, and throwing the schizothorax taliensis wild adults; conducting feeding with the cold water fishes or the schizothorax taliensis offspring seeds according to weights of the hucho bleekeri wild living bodies, and conducting feeding with the artificial nutrition feed additives containing glycine betaine, citric acid, sodium chloride, inosinic acid and complex microbial flora. The taming method of the wild hucho bleekeri living bodies has the advantages of being mild, relieving the stress reaction of the wild hucho bleekeri living bodies, maintaining the natural ferity of the wild hucho bleekeri living bodies, shortening the artificial taming transient time, and improving the taming rate of survival of the wild hucho bleekeri living bodies.

The invention provides an artificial high-efficiency clonal propagation method of dendrobium anosmum. The method comprises the following steps: inoculating a dendrobium anosmum stem explant with budssubjected to disinfection sterilization treatment into a culture medium A, and performing culturing under the conditions that the illuminance is 1500-2000lx and the illumination time is 10h/d; starting multiplication culture under the condition that the temperature is controlled to be 25 +/-1 DEG C, so that the base number of the material is increased, and the multiplication coefficient can be about 2.35 after 75 days; shearing the material into a stem segment containing 2-3 nodes, inoculating the stem segment into a culture medium B, and performing culturing for 60 days to form a multi-bud clustered system with a propagation coefficient of 6.0 or above; enabling adventitious roots to be synchronously induced in the culture medium, wherein the rooting rate reaches 100%; and the survival rate of rooted seedlings reaching 100% after the rooted seedlings are subjected to acclimatization for 60 days. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, provides technical support for protecting wild resources and breeding high-quality plantlets, and can produce excellent plantlets with consistent genotype backgrounds in a short time.