31results about How to "Improve domestication survival rate" patented technology

The present invention relate to a device for plant tissue liquid culture and controlling method thereof. The device comprises an incubator, a lamp box and a nutrient solution storing container. The incubator comprises a workroom, a regulation cabin and a controlling device. The workroom is provided with a liquid culture container, an air supply pipe, a temperature / moisture sensor, a water level sensor and a gas concentration sensor. A liquid feeding total pipeline and a liquid returning total pipeline are arranged between the nutrient solution storing container and the liquid culture container, the liquid feeding total pipeline is provided with a reflux valve and a water pump, the liquid returning total pipeline is provided with an electromagnetic valve, and each sensor is connected with the controlling device. The device described by the present invention uses liquid drive to replace gas drive, thus lowering energy consumption. The contact time of the nutrient and the culture are regulated by opening-closing of the electromagnetic valve. The quality of tissue culture plant is improved and the explant reaches the optimal growth, differentiation and metabolism states through controlling the microenvironment, thereby establishing carbolic sink-source relationship by regulating gas concentration. The integral body is of a box structure and can be produced in a large scale at any place and time.

The invention discloses a tissue culture propagation method for rhodiola sacra. The method comprises the following steps: collection and sterilization treatment of an explant: spraying carbendazol onto rhodiola sacra plants 13 to 15 days in advance, removing pest-free rhodiola sacra leaves from the rhodiola sacra plants, soaking the pest-free rhodiola sacra leaves in a washing powder solution for 20 minutes, rinsing with clear water for 2 hours, and performing explant sterilization treatment on the rhodiola sacra leaves in a super clean bench; adventitious bud induction: cutting the disinfected and sterilized rhodiola sacra leaves obtained in the step I into small pieces in a sterile environment, and inoculating the small pieces onto a sterilized culture medium for inducing rhodiola sacra calluses to obtain the rhodiola sacra calluses; rooting culture: cutting adventitious buds growing to 1cm from the calluses, inoculating the adventitious buds onto a rooting culture medium, and performing rooting culture for 45 to 60 days to obtain rhodiola sacra aseptic seedlings; acclimatizating and transplanting: transplanting rooting aseptic seedlings into an outdoor greenhouse, growing for 7 days, separating the aseptic seedlings from the rooting culture medium, and planting in a mixed substrate.

The invention belongs to the technical field of rare and peculiar fish farming and particularly relates to a feed training method of wild rhinogobio ventralis. The feed training method includes a hanger phase, an artificial inducing phase and a complete mixed-feed feeding phase; the artificial inducing phase includes two stages, one is a complete live-food feeding stage, and the other is an artificial mixed-feed inducing stage. With the method, the technical problem of difficult feed training of the wild rhinogobio ventralis under the condition of artificial breeding is successfully solved, the technical base of large-scale cultivation of parent fish of the rhinogobio ventralis is established, and large-scale propagation and large-scale releasing of the rhinogobio ventralis are made possible.

The invention discloses a cultivation method combining water cultivation and matrix cultivation of anthrium andraeanum tube seedling which is suitable for cultivation in the north area. The inventionis divided into the following steps: (1) water cultivation: under the air temperature of 16-30 DEG C, the relative moisture above 90 percent, the light intensity of 5000-10000Lux, the tube seedling isselected and the root thereof is dipped in oxygenated water with the temperature of 20-28 DEG C for 20-30 days after the cultivation solution is rinsed; (2) matrix cultivation: under the air temperature of 18-34 DEG C, the relative moisture above 60 percent and the light intensity of 10000-15000Lux, the seedling going through the step (1) is planted in the matrix containing peat, pearlite and a spot of organic fertilizer for cultivation. The method of the invention can promote new root to grow quickly, has high survival rate, is easy to manage and saves energy.

The invention discloses a method for hatching and breeding anser indicus. Aiming at the shortcomings that the hatching rate of the anser indicus by artificial hatching in the prior art is low and the method cannot be popularized in the actual breeding production, the invention provides a method for hatching and breeding the anser indicus of which the incubation hatching rate is over 90% under theconditions that the incubation temperature is 38 degrees centigrade + / - 0.1 degrees centigrade and the relative humidity is 56.0% + / - 2.0%. According to the method for artificial hatching and breeding the adult wild gooses in the invention, the adult wild gooses can be put in the breeding net to breed in open air, wherein the water area in the breeding net is 25% to 40% and the land area is 60% to 75%; the survival rate of the adult wild goose bred by the method reaches over 90%, the wild habits are good, and the ecological quality is high. The invention further provides a stereo breeding method of the anser indicus and plateau fish, which fully realizes the circulation economic model by matching a stereo breeding technology of breeding the wild gooses at the upstream and breeding the fish at the downstream with forage plant and methane utilization, and improves the overall earnings of the plateau breeding industry. The method has rational theory and simple method, and can easily control the artificial implementation conditions, so the method is suitable for popularizing the plateau area economic development.

The invention provides a method for taming wild Lhasa Schizothorax. Parent fish is collected with an electric fishing method, the parent fish which is well-developed, vivacious and robust in health is selected as a taming object, potassium permanganate is used for disinfection, high protein dead bait and buoyancy pellet feed are selected and fed alternatively for taming, and the taming survival rate is higher. The taming method is simple, the operability is high, the piratical applicability is good, large-scale and standard taming of wild Lhasa Schizothorax can be realized, development of the Lhasa Schizothorax is promoted, and the wild wild Lhasa Schizothorax resource is effectively protected.

The invention relates to a pinellia ternate seedling tissue culturing, hardening and domesticating substrate and a preparation and use method for the same. The substrate is made from fine river sand, humus soil, perlite and composite vitamin B. The preparation method is that fine river sand and humus soil are screen and disinfected and then mixed with the perlite; at last the composite vitamin B is added. The use method is that when pinellia ternate tissue culture seedlings grow to 5 to 8cm, sterile water is sprayed and the seedlings are placed in a culture room for 2 to 3 days; then the seeds are taken out and roots are cleaned; the roots are immersed into 0.1mg / l naphthylacetic acid water solution for 10 seconds and then transplanted to the pinellia ternate seedling tissue culturing, hardening and domesticating substrate in a small shed with 40 to 60% of sunshade rate; the seedlings are hardened and domesticated for one month at the temperature of 40 to 25 DEG C and with humidity of 40 to 50%; and then the seedlings can be transplanted to fields. The raw material for the substrate can be easily accessed; raw material ratio is scientific and rational as well as effective; and domestic survival rate of the pinellia ternate seedlings can be improved by 96% or more.

The invention relates to a wild pampus argenteus seedling acclimatizing method. The wild pampus argenteus seedling acclimatizing method includes feeding by stages. By means of feeding live baits and mixed feed, eating habits and digestive system of wild pampus argenteus can be changed step by step, batches of juvenile pampus argenteus are prevented from dying from flatulence caused by dyspepsia caused by bait change. The wild pampus argenteus seedling acclimatizing method has the advantages that survival rate and growing speed of artificial cultured pampus argenteus changed from the wild pampus argenteus can be increased effectively.

The invention discloses a two-step rooting method of a globe artichoke variety LORCA. The method comprises the following steps of 1 rooting culture medium preparing, 2 tissue culture seedling culture condition setting, 3 rooting culturing, 4 seedling hardening and 5 transplanting. The method has the advantages that globe artichoke tissue culture seedlings are rooted through the two-step rooting method, root system induction and growth are separated, and culture medium formulas used in all the steps are all initiated in globe artichoke tissue culture. By using white kraft paper, the humidity in a bottle is greatly reduced, and a vitrification phenomenon is effectively constrained. The kraft paper is very good in breathability, is mostly applied to bagging breeding of crops such as rice and rapes and can effectively prevent outside mould and the like from entering bags, and therefore bottle seedlings cannot be contaminated. By increasing the dosage of agar powder and conducting shading treatment, the vitrification rate can be effectively decreased, and the rooting rate can be effectively increased; the rooting rate is increased to 86% from 20%, and the bottle seedling domestication survival rate is increased to 92% from 65%.