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Liquid rapid-propagation method for rhizoma bletillae seeds

A fast, liquid technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long cycle, cumbersome tissue culture operation, low reproduction efficiency, etc., achieve low cost, reduce cultivation time, The effect of improving reproductive efficiency

Active Publication Date: 2015-11-25
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the problems of low breeding efficiency, cumbersome tissue culture operation and long cycle of Radix Radix, and provide a rapid liquid propagation method for Radix Radix Radix Seedlings, which can industrialize the propagation of Radix Radix Radix Seedlings in a short period of time. The seedlings needed for large-scale production can be obtained within the plant, and it also has the characteristics of low cost, neat growth of seedlings, and high survival rate of domesticated seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Selection and disinfection of explants: Select the mature fruit pods (from mid-September to mid-November) of Anhui Zihuabai as explants, wash the surface dirt with washing powder, and use Sterilize with 75% ethanol for 2 minutes, pour out the ethanol and wash with sterile water 3 times. Disinfect with 0.1% mercury liter solution for 25 minutes, and wash with sterile water 3 times. Pollution rate is less than 5%.

[0032] (2) Germination of seeds: Spread the seeds of Baiji evenly on the surface of 1 / 2MS liquid medium. There are 300 seeds per bottle, the temperature is 26°C, the light intensity is 3000Lx, the light time is 16 hours / day, and the cultivation time is 60 days. Seed germination rate is greater than 90%.

[0033] (3) Seedling cultivation: transfer the floating Baiji seedlings from the mother bottle to MS+2.0mg / L6-BA (commercially available)+1.0mg / LIAA (commercially available)+banana puree (commercially available) 80g / L In the solid medium (pH5.6-6.4), t...

Embodiment 2

[0036] (1) Selection and disinfection of explants: Select the mature fruit pods (from mid-September to mid-November) of Anhui Zihuabai as explants, wash the surface dirt with washing powder, and use Sterilize with 75% ethanol for 3 minutes, pour out the ethanol and wash with sterile water 3 times. Disinfect with 0.1% mercury liter solution for 20 minutes, and wash with sterile water 5 times. Pollution rate is less than 5%.

[0037] (2) Germination of seeds: Spread the seeds of Baiji evenly on the surface of 1 / 2MS liquid medium. There are 400 seeds per bottle, the temperature is 26°C, the light intensity is 3500Lx, the light time is 14 hours / day, and the cultivation time is 45 days. Seed germination rate is greater than 90%.

[0038] (3) Seedling cultivation: transfer the floating Baiji seedlings from the mother bottle to the solid medium of MS+1.0mg / L6-BA+2.0mg / LIAA+banana puree 60g / L (pH5.6-6.4), The culture temperature is 28°C, the light intensity is 3500Lx, the light ti...

Embodiment 3

[0041] (1) Selection and disinfection of explants: Select the mature fruit pods (from mid-September to mid-November) of Anhui Zihuabai as explants, wash the surface dirt with washing powder, and use Sterilize with 75% ethanol for 2 minutes, pour out the ethanol and wash with sterile water 3 times. Disinfect with 0.1% mercury liter solution for 25 minutes, and wash with sterile water 4 times. Pollution rate is less than 5%.

[0042] (2) Germination of seeds: Spread the seeds of Baiji evenly on the surface of 1 / 2MS liquid medium. There are 350 seeds per bottle, the temperature is 26°C, the light intensity is 3000Lx, the light time is 15 hours / day, and the cultivation time is 50 days. Seed germination rate is greater than 90%.

[0043] (3) Seedling cultivation: transfer the floating Baiji seedlings from the mother bottle to the solid medium of MS+3.0mg / L6-BA+1.0mg / LIAA+100g / L of banana puree (pH5.6-6.4), The culture temperature is 28°C, the light intensity is 3000Lx, the ligh...

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Abstract

The invention discloses a liquid rapid-propagation method for rhizoma bletillae seeds. The method comprises the following steps: (1) explant selection and disinfection; (2) seed germination and young-seedling culture; (3) adult-seedling culture; and (4) seedling hardening and transplantation. The step of seed germination and young-seedling culture concretely comprises the sub-step of culturing the rhizoma bletillae seeds in a liquid medium, wherein culture conditions are as follows: temperature is 26+ / -2 DEG C; illumination intensity is 3000 to 3500 Lx; illumination time is 14 to 16 hours / day; and culture time is 45 to 60 days. The liquid rapid-propagation method for the rhizoma bletillae seeds, provided by the invention, can realize industrialization of rhizoma bletillae seedling propagation and obtains rhizoma bletillae seedlings meeting the needs of large-scale production in a short time; meanwhile, the method also has the characteristics of low cost, neat seedling growing, high seedling-hardening domestication survival rate, etc.

Description

technical field [0001] The invention relates to a method for rapid propagation of plants, in particular to a method for rapid propagation of seeds in liquid. Background technique [0002] The tissue culture technique of higher plants refers to separating a part of the plant body through aseptic operation, inoculating it on the medium, and cultivating it under artificially controlled conditions to make it produce a complete plant. Tissue culture is a new technology developed in this century. Due to the advancement of science and technology and the wide application of exogenous hormones, tissue culture not only provides a reliable experimental basis for related disciplines in theory, but also becomes a large-scale, A new method for batch factory production of seedlings. Plant tissue culture has the advantages of artificial control of culture conditions, short growth cycle, high reproduction rate, low cost, better preservation of maternal traits, convenient management, and is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 丁志山蒋福升钱朝东丁兴红程东庆陈铌铍高承贤李美芽鲁光耀杨仙
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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