Culture medium for improving domestication survival rate of euphorbia hirsuta tissue culture seedlings and domestication method thereof

Abstract

The invention relates to the field of medicinal plant tissue culture and traditional Chinese medicinal materials, in particular to a culture medium for improving the domestication survival rate of euphorbia hirsuta tissue culture seedlings and a domestication method of the euphorbia hirsuta tissue culture seedlings. The culture method comprises the steps of explant disinfection, cluster bud induction, cluster bud proliferation, cluster bud rooting and rooting seedling domestication. According to the method, during rooting, low-concentration 2, 4-D and IBA are matched, rooting of euphorbia pekinensis can be effectively promoted, the rooting rate can be close to 100%, meanwhile, 2.0-2.4 mu g/L of silver nitrate and 0.2-0.3 mg/L of MET are added into a rooting bottle culture medium, the vitrification phenomenon of rooted seedlings can be completely inhibited, and the domestication survival rate can be increased to a great extent.

Description

technical field [0001] The invention relates to the field of tissue culture of medicinal plants and Chinese medicinal materials, in particular to a medium for improving the domestication and survival rate of spurge sprigs tissue culture seedlings and a domestication method thereof. Background technique [0002] Knoxia valerianoides Thorelet Pitard, also known as Knoxia valerianoides Thorelet Pitard, also known as Knoxia valerianoides Thorelet Pitard, is a perennial herb of the Rubiaceae family, mainly growing on hillside grasslands at an altitude of 50 to 1500 meters. , my country is distributed in Yunnan, Guangdong, Fujian, Hainan, Guangxi, Zhejiang and other provinces and regions, and foreign countries are also distributed in India, Myanmar, Cambodia, Nepal, Thailand and other places. [0003] The red spurge root is used as medicine, which has the effect of diarrhoea and drinking, reducing swelling and dispersing knots. It is mainly used for the treatment of edema and fulln...

AI Technical Summary

Problems solved by technology

At present, the main source of red euphorbia medicinal materials is wild resources, and the market demand is expanding year by year, which makes the price of red euphorbia increase rapidly, resulting in the disorderly mining of wild resources

In recent years, in Yunnan, Guangxi, Guangdong and other provinces and cities, the artificial planting of red euphorbia has been gradually carried out. At present, the propagation technology of red euphorbia mainly relies on seed propagation. Very low seed germination rate

According to the introductions of various big growers in Baoshan and Chuxiong in Yunnan, the germination rate of red euphorbia seeds under artificial conditions does not exceed 0.01%. At the same time, the market price of red euphorbia is positively correlated with the content of active ingredients such as euphorbia and euphorbia , while the quality of germplasm is uneven and the genetic traits are unstable when propagated by seeds

[0005]In recent years, Guangxi University, Guangxi Medicinal Botanical Garden, Institute of Biology of Guangxi Academy of Sciences, Hubei Three Gorges Institute and other scientific research institutes have launched Euphorbia and related tissue culture techniques However, under the existing technical conditions, the tissue culture breeding technology of Euphorbia euphorbia has low multiplication coefficient, extremely serious vitrification in the process of multiplication and rooting, extremely fast dehydration of rooted seedlings after they come out of the bottle, and low domestication and survival rate of rooted seedlings.

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