Culture medium for improving domestication survival rate of euphorbia hirsuta tissue culture seedlings and domestication method thereof
A culture medium formula and culture medium technology are applied to the culture medium for improving the survival rate of tissue culture seedlings of Euphorbia chinensis and the field of domestication, and can solve the problems of low domestication survival rate of rooted seedlings, disorderly excavation of wild resources, and low proliferation coefficient. and other problems, to achieve the effect of improving the survival rate of domestication, inhibiting the occurrence of vitrification, and improving the multiplication coefficient
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Embodiment 1
[0027] A method for improving the survival rate of domestication of spurge tissue culture seedlings, comprising the following steps:
[0028] 1. Cleaning of the explants: Transplant the spurge in the room, spray the stems and leaves with 1000 times of 75% carbendazim every 3 days, and take the stem section within 30 cm of the top of the spurge after spraying 3 times. , after removing the leaves, cut it into 2cm small sections with buds, rinse with saturated soapy water solution for 10min, rinse with tap water, add 2 drops of Tween-80, shake for 15min, and rinse with tap water for 50min;
[0029]2. Disinfection of explants: After washing the explants, transfer them to a clean bench, rinse with 75% (v / v) alcohol for 10s, rinse twice with sterile water, and rinse with 8% (v / v) hydrogen peroxide solution for 10 minutes , rinsed with sterile water for 3 times, sterilized with 0.4% (v / v) sanjierzil solution for 40 minutes, rinsed with sterile water for 4 times;
[0030] 3. Inductio...
Embodiment 2
[0035] A method for improving the survival rate of domestication of spurge tissue culture seedlings, comprising the following steps:
[0036] 1. Cleaning of the explants: Transplant the spurge in the room, spray the stems and leaves with 1000 times of 75% carbendazim every 3 days, and take the stem section within 30 cm of the top of the spurge after spraying 3 times. , after removing the leaves, cut it into 3cm small sections with buds, rinse with saturated soapy water solution for 15min, rinse with tap water, add 3 drops of Tween-80, shake for 20min, and rinse with tap water for 60min;
[0037] 2. Disinfection of explants: After washing the explants, transfer them to a clean bench, rinse with 75% (v / v) alcohol for 15s, rinse with sterile water for 3 times, and 8% (v / v) hydrogen peroxide solution for 15 minutes , rinsed 4 times with sterile water, sterilized with 0.4% (v / v) sanjierzil solution for 45min, rinsed with sterile water 5 times.
[0038] 3. Induction of clump buds: ...
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