A kind of hardening and acclimatization substrate of pinellia tissue cultured seedlings and its preparation and use method

A technology of tissue culture seedlings and pinellia, applied in the field of medium for hardening and domestication of pinellia tissue culture, can solve the problems of high cost, hardening of pinellia tissue culture, undetermined domestication links, etc., and achieve Promote root growth and development, improve domestication survival rate, and improve the effect of organic matter supply

Inactive Publication Date: 2017-08-25
河南绿普森农林科技有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1970s, with the gradual deepening of the research on the medicinal efficacy of pinellia, the market demand for pinellia has been increasing. The destructive mining of wild resources of pinellia has been far from meeting the market demand, resulting in the price of pinellia rising year after year. , Pinellia fakes are flooding the market
In order to solve the contradiction between supply and demand of pinellia, since the 1980s, we have started to explore wild varieties of pinellia and artificial cultivation techniques, and have accumulated rich experience. At present, the propagation of pinellia is mainly based on the asexual reproduction of tubers and bulbils. Mainly, but the amount of seeds used is large, and the reproduction coefficient is very low, the cost is also high, and the emergence rate of seed propagation is low, so it is not suitable for promotion in production
[0003] In order to improve the reproductive coefficient of Pinellia, adapt to the current standardized planting technical requirements, and lay the foundation for the research on the metabolic engineering of Pinellia, the use of tissue culture and rapid propagation to obtain Pinellia seedlings has received attention; How to obtain Pinellia tissue culture seedlings through explant screening, callus induction, detoxification treatment and other technologies, but there is no definite technology for the hardening and domestication of Pinellia tissue cultured seedlings; One of the technical bottlenecks of whether the tissue-cultured seedlings of Pinellia can enter transplanting and large-scale artificial planting
For the hardening and domestication of Pinellia tissue culture seedlings, there is no unified domestication substrate at present, and many of them are domesticated directly with a single sand base, but the effect is very poor and the survival rate is extremely low

Method used

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  • A kind of hardening and acclimatization substrate of pinellia tissue cultured seedlings and its preparation and use method

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preparation example Construction

[0015] The preparation method for hardening seedlings and domestication substrates of the Pinellia tissue culture seedlings comprises the following steps:

[0016] (1) Screen the fine river sand with an average particle size of 0.25-0.35mm and a fineness modulus of 1.6-2.2, and sterilize the screened fine river sand at 121°C for 20 minutes, cool to room temperature, and set aside ;

[0017] (2) Pass the humus soil through a 60-mesh sieve to remove waste that is too thick or too fine, and then sterilize it through high-temperature or high-temperature distillation, cool it to room temperature, and set aside;

[0018] (3) Take the fine river sand and humus soil treated in the above steps (1) and (2) according to the volume percentage, and mix them to obtain the mixture A; add the perlite according to the volume percentage to the obtained mixture A , mix evenly again, and finally add complex vitamin B and mix to obtain Pinellia tissue culture seedling hardening and acclimatizatio...

Embodiment 1

[0021] A medium for cultivating and acclimating Pinellia tissue culture seedlings, the raw materials of which are fine river sand, humus, perlite and complex vitamin B, wherein the volume percentage of fine river sand, humus and perlite is: fine river sand 10%, humus 50%, perlite 40%, and the quality of complex vitamin B is 0.01% of the total mass of fine river sand, humus and perlite. The composition of the complex vitamin B is: every 100mg portion of the complex vitamin B includes, vitamin B 1 30mg, vitamin B 2 20mg, vitamin B 6 20mg, vitamin B 9 15mg, vitamin B 12 10mg and cobalamin 5mg.

[0022] The preparation method for hardening seedlings and domestication substrates of the Pinellia tissue culture seedlings comprises the following steps:

[0023] (1) Screen the fine river sand with an average particle size of 0.25mm and a fineness modulus of 1.6, and then sterilize the screened fine river sand at 121°C for 20 minutes with damp heat for use;

[0024] (2) Pass t...

Embodiment 2

[0028] A medium for cultivating and acclimating Pinellia tissue culture seedlings, the raw materials of which are fine river sand, humus, perlite and complex vitamin B, wherein the volume percentage of fine river sand, humus and perlite is: fine river sand 20%, humus 40%, perlite 40%, the quality of complex vitamin B is 0.02% of the total mass of fine river sand, humus and perlite. The composition of the complex vitamin B is: every 100mg portion of the complex vitamin B includes, vitamin B 1 30mg, vitamin B 2 20mg, vitamin B 6 20mg, vitamin B 9 15mg, vitamin B 12 10mg and cobalamin 5mg.

[0029] The preparation method for hardening seedlings and domestication substrates of the Pinellia tissue culture seedlings comprises the following steps:

[0030] (1) Screen the fine river sand with an average particle size of 0.35mm and a fineness modulus of 2.2, and then sterilize the screened fine river sand at 121°C for 20 minutes with damp heat for use;

[0031] (2) Pass the h...

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Abstract

The invention relates to a pinellia ternate seedling tissue culturing, hardening and domesticating substrate and a preparation and use method for the same. The substrate is made from fine river sand, humus soil, perlite and composite vitamin B. The preparation method is that fine river sand and humus soil are screen and disinfected and then mixed with the perlite; at last the composite vitamin B is added. The use method is that when pinellia ternate tissue culture seedlings grow to 5 to 8cm, sterile water is sprayed and the seedlings are placed in a culture room for 2 to 3 days; then the seeds are taken out and roots are cleaned; the roots are immersed into 0.1mg / l naphthylacetic acid water solution for 10 seconds and then transplanted to the pinellia ternate seedling tissue culturing, hardening and domesticating substrate in a small shed with 40 to 60% of sunshade rate; the seedlings are hardened and domesticated for one month at the temperature of 40 to 25 DEG C and with humidity of 40 to 50%; and then the seedlings can be transplanted to fields. The raw material for the substrate can be easily accessed; raw material ratio is scientific and rational as well as effective; and domestic survival rate of the pinellia ternate seedlings can be improved by 96% or more.

Description

technical field [0001] The invention relates to a pinellia group substrate, in particular to a substrate for hardening and domesticating pinellia tissue culture seedlings and its preparation and use method. Background technique [0002] pinellia Pinellia ternata (Thunb.) Breit. is a plant of the genus Pinellia in the family Araceae. It is excavated in summer and autumn, and the dried tubers are used as medicine. It is an important traditional Chinese medicinal material and export material. Efficacy such as eliminating ruffian and resolving stagnation. For wet phlegm and cold phlegm, cough with dyspnea and excessive phlegm, phlegm-retention dizziness and palpitation, wind-phlegm dizziness, phlegm syncope headache, vomiting and regurgitation, fullness in the chest cavity, plum nucleus qi; external treatment of carbuncle, swollen phlegm nucleus and other diseases. From the beginning of the 1950s to the end of the 1960s, Pinellia pinellia was mainly used to excavate wild reso...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G9/10A01G1/00C05G3/00
CPCC05D9/00C05G3/00C05F11/02
Inventor 侯典云胥华伟张改娜赵杏利张利霞杨萌萌
Owner 河南绿普森农林科技有限公司
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