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Two-step rooting method of globe artichoke variety LORCA

A two-step rooting and artichoke technology, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as vitrification, and achieve the effects of increasing rooting rate, increasing dosage, and improving domestication and survival rate.

Active Publication Date: 2017-01-04
爱可道生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a two-step rooting method for artichoke variety LORCA (Lauca), the purpose of which is to solve the problem of vitrification in the existing artichoke tissue culture method, and improve the rooting rate, cultivate strong seedlings, so as to improve the growth rate of bottle seedlings. Survival rate

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0052] A two-step rooting method for an artichoke variety LORCA (Lauca), comprising the steps of:

[0053] (1) Preparation of rooting medium

[0054] (1.1) Mother liquor preparation and storage

[0055] Choose chemically pure or analytically pure chemicals, according to the formula of MS medium, the amount of elements is 20 times, of which C a Cl 2 Separately dubbed 40

[0056] 100 times for trace elements; 100 times for iron salts; 100 times for organic matter, 100 times for inositol alone; 20 times for L-tyrosine, and 0.1mg·mL for NAA -1 , 6-BA formulated into 0.1mg·mL -1, Store all stock solutions in a refrigerator at 4°C;

[0057] (1.2) Culture medium preparation

[0058] The first rooting medium: 1 / 2MS+NAA1.0mg·L -1 +6-BA0.6mg·L -1 + L-tyrosine 50mg·L-1 +Activated carbon 2.0g·L -1 + Inositol 50mg·L -1 , agar powder 7 g·L -1 , white sugar 20 g·L -1 , pH 5.5;

[0059] The second step rooting medium: MS+inositol 50mg·L -1 , agar 7 g·L -1 , white sugar 40 g·L ...

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PUM

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Abstract

The invention discloses a two-step rooting method of a globe artichoke variety LORCA. The method comprises the following steps of 1 rooting culture medium preparing, 2 tissue culture seedling culture condition setting, 3 rooting culturing, 4 seedling hardening and 5 transplanting. The method has the advantages that globe artichoke tissue culture seedlings are rooted through the two-step rooting method, root system induction and growth are separated, and culture medium formulas used in all the steps are all initiated in globe artichoke tissue culture. By using white kraft paper, the humidity in a bottle is greatly reduced, and a vitrification phenomenon is effectively constrained. The kraft paper is very good in breathability, is mostly applied to bagging breeding of crops such as rice and rapes and can effectively prevent outside mould and the like from entering bags, and therefore bottle seedlings cannot be contaminated. By increasing the dosage of agar powder and conducting shading treatment, the vitrification rate can be effectively decreased, and the rooting rate can be effectively increased; the rooting rate is increased to 86% from 20%, and the bottle seedling domestication survival rate is increased to 92% from 65%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a two-step rooting method for an artichoke variety LORCA. Background technique [0002] LORCA is a processed artichoke variety imported from France in Changde City in 2005. The flower buds of this variety are ovoid (between spherical and olive-shaped), the bracts are green, the buds are firm, fresh and tender, and the edible rate is high. Strong budding, good quality, suitable for processing into cans. The whole growth period is about 240 days, and the harvest period is 60 days. It was registered and recognized in Hunan Province in 2014, and has now become the main variety in the local area. Like other artichoke varieties, LORCA only blooms but does not bear fruit in my country, and cannot be self-sustained, so it needs to be purchased from abroad every year. Artichoke seeds are expensive, with an average of 0.4-0.5 yuan per seed, and often delay the farming time....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 王中美张平喜王桢李树举吴亚飞周娟夏继国陈祖宏尤龙威
Owner 爱可道生物科技有限公司
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