Preparation method of large cherry stock Gisela plant tissue culture medium

Abstract

The invention relates to a preparation method of a large cherry stock Gisela plant tissue culture medium, belonging to the technical field of agricultural biology. The large cherry stock Gisela plant tissue culture medium comprises a differentiation medium and a rooting medium, wherein the two culture mediums are respectively prepared and applied in a matching way; a formula of the culture mediumis as follows: a basic culture medium 10 times MS, 6-benzylaminopurine, indole-3-butyric acid, cane sugar and agar powder; the formula and the proportion of the rooting medium are as follows: a basicculture medium 10 times 1 / 2 MS, 1-naphthylacetic acid, the indole-3-butyric acid, 2,4-dichlorphenoxyacetic acid, the cane sugar and the agar powder; and the differentiation medium and the rooting medium are respectively prepared and applied in a matching way. The preparation method of the differentiation medium and the rooting medium can shorten the tissue culture plantlet culturing period almostwithout variation, has high plantlet uniformity and rooting rate and normal roots without wound between roots and plantlets, can greatly enhance the acclimatization survival rate through the cooperation of the two culture mediums and reduce the cost of tissue culture plantlets.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a formula for differentiation and rooting of plant tissue culture. Background technique [0002] Known as "the first branch of spring fruit", the big cherry is sweet and fragrant. It is an earlier fruit tree among deciduous fruit trees in the north. In recent years, the planting area of ​​big cherry has expanded rapidly. For large cherry growers, the choice of rootstock directly affects the quality and yield of the fruit. Based on this situation, Gisela No. 5 is currently the best rootstock, so the demand for large cherry rootstock Gisela No. 5 is rapid increase. At present, in the society, most of the large cherry rootstock Gisela No. 5 is mainly propagated by tissue culture and rapid propagation. In other tissue culture rooms, due to the unsuitable formula of differentiation and rooting medium, the differentiation multiple is not high, only about 2 times. ...

AI Technical Summary

Problems solved by technology

[0003] The purpose of this invention is to provide a kind of preparation method of large cherry rootstock Gisela plant tissue culture medium, to solve the unreasonableness of the culture medium components in the prior art, the differentiation multiple of bottle seedlings is not high, and the propagation speed is slow; the rooting quality is not good, the composition Low survival rate of domesticated seedlings, increased production cost of seedlings, etc.