Apple cold-resistant Russian rootstock 176 plant tissue culture medium preparation method

Abstract

The invention discloses an apple cold-resistant Russian rootstock 176 plant tissue culture medium preparation method, wherein the components of the proliferation culture medium formula comprise a basic culture medium MS, 0.5-1.0 mg / L 6-benzylaminoadenine, 0.3-1.0 mg / L indole-3-butyric acid, 0.5-1.0 g / L active carbon, 20-40 g / L sucrose, and 3.5-4.5 g / L agar powder, and the components of the rootingculture medium formula comprise a basic culture medium 1 / 2MS, 1.0-1.5 mg / L indole-3-butyric acid, 0.5-1.5 mg / L naphthalene acetic acid, 20-40 g / L sucrose, and 3.5-4.5 g / L agar powder. According to the present invention, the culture period can be substantially shortened through the proliferation culture medium formula, and with the rooting culture formula, the rooting rate is high, wherein the rooting rate is 85-88% at the 16th day after inoculation, the number of the roots growing on each seedling is 4-7, especially the root directly grows from the base portion of the seedling, no callus exists between the root and the seedling, and the growing root is the normal root, not the puffed roots, such that the survival rate of the acclimation is substantially improved due to the two characteristics so as to reduce the cost of the tissue culture seedling.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for configuring a plant tissue culture medium. Background technique [0002] At present, in the society, most of the propagation methods of Russian Anvil 176 are based on tissue culture and rapid propagation. In other tissue culture rooms, due to the inappropriate formulation of the proliferation medium and rooting medium, the differentiation multiple is not high, only about 2 times. Therefore, Propagation speed is slow; browning phenomenon is easily produced during the multiplication period; and the root quality of rooted seedlings is not good. The nutrient composition is not smooth; some roots are a kind of puffed root, which can not fully exert the absorption capacity of the root. These two situations greatly reduce the survival rate of tissue culture seedlings domestication, thus causing Russian anvil 176 tissue culture Seedling cost increases, no matte...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is exactly in order to solve above-mentioned problem, provides a kind of plant tissue culture medium configuration method, to solve existing culture medium formula unreasonable, bottle seedling differentiation multiple is not high, propagation speed is slow; Low survival rate, increased seedling production costs and other issues; at the same time solve the problem that apple rootstocks are needed in some cold regions