Apple cold resistance Russian rootstock 396b plant tissue culture medium configuration method

Abstract

The invention discloses an apple cold resistance Russian rootstock 396b plant tissue culture medium configuration method. A proliferation medium formula is prepared from the following components witha proportion: a basic culture medium MS, 0.5-0.8mg / L of 6-benzylaminopurine, 0.8-1.5mg / L of indole-3-butyric acid, 20-40g / L of sucrose and 3.5-4.5g / L of agar powder; and a rooting medium formula is prepared from the following components with a proportion: a basic culture medium 1 / 2 MS, 0.8-1.5mg / L of indole-3-butyric acid, 1.0-2.0mg / L of 1-naphthylacetic acid, 20-40g / L of sucrose and 3.5-4.5g / L ofthe agar powder. by adopting the proliferation medium formula, the culture period can be greatly shortened, one generation needs about 30 days only, the differentiation multiple is between 5 and 7, abud can reproduce hundreds of billions of seedlings in one year with hardly any variation, and the uniformity of the produced seedlings is high; and the rooting medium formula is high in rooting ratio, the rooting ratio is between 87 percent and 92 percent 15 days after inoculation, the rooting amount of each seedling is between 4 and 6, particularly, roots are directly produced from the base ofeach seeding without callus between the roots and each seeding, and the produced roots are normal roots not extruding roots, so that the two characteristics can greatly increase the domestication survival rate, and the seedling culture cost is lowered.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for configuring a plant tissue culture medium. Background technique [0002] At present, in the society, most of the propagation methods of Russian Anvil 396 are based on tissue culture and rapid propagation. In other tissue culture rooms, due to the inappropriate formulation of the proliferation medium and rooting medium, the differentiation multiple is not high, only about 2 times. Therefore, The propagation speed is just slow; and the root quality of rooted seedlings is not good, and what have is to grow callus first from seedling incision, and then take root from callus, causes the upper and lower parts of seedling to transport nutrient composition unimpededly like this; The root is a kind of puffed root, which cannot give full play to the absorption capacity of the root. These two conditions greatly reduce the survival rate of the domesticated seedlings...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is exactly in order to solve above-mentioned problem, provides a kind of plant tissue culture medium configuration method, to solve existing culture medium formula unreasonable, bottle seedling differentiation multiple is not high, propagation speed is slow; Low survival rate, increased seedling production costs and other issues; at the same time solve the problem that apple rootstocks are needed in some cold regions