Preparation method for plant tissue culture mediums used for dwarf apple rootstock M26

Abstract

The invention discloses a preparation method for plant tissue culture mediums used for dwarf apple rootstock M26. A formula for a proliferation culture medium comprises a basic culture medium MS, 6-benzylamino adenine with a concentration of 0.5 to 0.8 mg/L, 3-indolebutyric acid with a concentration of 0.3 to 0.5 mg/L, D-calcium pantothenate with a concentration of 0.1 to 0.3 mg/L, cane sugar witha concentration of 20 to 40 g/L and agar powder with a concentration of 3.5 to 4.5 g/L. A formula for a rooting medium comprises a basic culture medium 1/2 MS, 3-indolebutyric acid with a concentration of 1.0 to 1.5 mg/L, D-calcium pantothenate with a concentration of 0.1 to 0.3 mg/L, 1-naphthylacetic acid with a concentration of 1.0 to 2.0 mg/L, cane sugar with a concentration of 20 to 40 g/L and agar powder with a concentration of 3.5 to 4.5 g/L. The formula for the proliferation culture medium allows a culture period to be substantially shortened, prevents occurrence of variation and enables produced seedlings to be high in uniformity. The formula for the rooting medium has high rooting rate; after inoculation for 15 days, the rooting rate is in a range of 87 to 95%; the number of grown roots of each seedling is in a range of 5 to 8; in particular, the roots directly grow out from the basal parts of seedlings, calluses are not formed between roots and seedlings, and normal roots instead of puffed roots are grown; so the survival rate of domestication can be substantially improved, and cost for tissue culture seedlings can be thus reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for configuring a plant tissue culture medium. Background technique [0002] At present, the propagation method of apple dwarf rootstock M26 mainly adopts tissue culture and rapid propagation. However, in some tissue culture rooms, due to the inappropriate formulation of the proliferation medium and rooting medium, the differentiation factor is not high, only about 2 times, so the propagation speed is slow; The callus grows first, and then takes root from the callus, so that the upper and lower parts of the seedlings cannot conduct nutrients smoothly; some roots are a kind of puffed root, which cannot fully exert the absorption capacity of the root. This situation has greatly reduced the survival rate of tissue-cultured seedling domestication, thereby causing the cost of apple dwarf rootstock M26 tissue-cultured seedlings to increase, no matter from quality...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is exactly in order to solve above-mentioned problem, provides a kind of plant tissue culture medium configuration method, to solve existing culture medium formula unreasonable, bottle seed