288 results about "Naphthalene acetic acid" patented technology

A method for directly seedling dendrobe seedlings by using a plastic greenhouse comprises the following steps: constructing a greenhouse; making a seedling bed; preparing a base material and performing sterilization, which can be realized by mixing pine barks and sheep manure, filtering the pine barks and the sheep manure through a screen after completely fermenting the pine barks and the sheep manure, soaking the pine barks and the sheep manure by using carbendazim, spreading the pine barks and the sheep manure on the seedling bed, placing the pine barks and the sheep manure on the seedling bed for 5-10 days, and controlling the water content; inoculating the symbiotic bacteria, which can be realized by taking a two years old base material of the dendrobe and uniformly scattering the two years old substrate on the seedling bed on which a new substrate is laid; pre-treating seeds, which can be realized by completely moisturizing the seeds by using sterile water containing naphthyl acetic acid; sterilizing the seeds in the greenhouse, which can be realized by fumigating every cubic meter of seeds by using 4 g mushroom protector, fumigating the seeds for one time before sowing, and fumigating the seeds every half a month in the future; scattering, which can be realized by after the seeds are scattered and before the seedlings are transplanted, controlling temperature in the greenhouse, water content of the seedling bed and air humidity, treating the seeds by weak lights within the first month of the scattering, and gradually enhancing illumination after budding; managing water, fertilizers and diseases of the seedlings; and transplanting the seedlings. The method for directly seedling the dendrobe seedlings by using the plastic greenhouse, provided by the invention has the advantages that as the dendrobe are transplanted after directly becoming to the seedlings, the seedling breeding cost is effectively reduced, the seedling rate of the seeds is more than 90 percent, and the transplanting survival rate of the seedlings is 100 percent.

The invention relates to plant wound liquid containing chitosan oligosaccharide, which is characterized by comprising a plant growth regulator, wherein the regulator is one or more of naphthalene acetic acid and humic acid and accounts for 1 to 50 percent of the total weight of the liquid; and the chitosan oligosaccharide accounts for 0.0015 weight percent to 0.03 weight percent of the total weight of the liquid. The liquid can be prepared into any liquid preparation for spraying. One to five days before plants are trimmed, grafted or girdled, the liquid is used for spraying a wound. The liquid is used for apple trees, pear trees, peach trees, jujube trees, grape vines, mango trees, longan trees, lychee trees, mandarin trees, watermelons, gingko trees, lonicera japonica, jasmine, Chinese rose and the like.

The invention belongs to the field of regulation and control of plant growth, and particularly relates to a plant growth regulator composition for regulating and controlling the treetop growth of a fruit tree. The plant growth regulator composition comprises the following effective components used for regulating and controlling the plant growth: prohexadione calcium, compound sodium nitrophenolate / diethyl aminoethyl hexanoate / sodium naphthalene acetate / triacontanol / brassinolide / 6-benzyladenine; the plant growth regulator composition is diluted to the concentration of 150-800 mg / kg by being blended with water in terms of effective components to be used for regulating and controlling the growth of the fruit tree. Compared with the prior art, the plant growth regulator composition disclosed by the invention solves the problem that treetop control, flower promotion and safety yield increase are difficult to synchronize in a fruit tree treetop control process, induces the nutrient elements to be transmitted and concentrated to fruits by regulating the ratio of the growth hormone, cytokinin and gibberellin of the fruit tree during a growth period, enhances the flowering rate, enhances the fruiting rate, achieves the effects of inhibiting vegetative growth and promoting reproductive growth, is obvious in yield increase effect and outstandingly improves the quality of the fruits.

The invention discloses an efficient tissue culture and rapid propagation technology for seedlings of bletilla striata. According to the technology, bletilla striata seeds are sown on a seed germination culture medium for sterile germination, the obtained seedlings are inoculated to a multiplication and subculture medium one by one for multiplication and subculture, clustering seedlings are obtained and inoculated to a rooting culture medium for rooting culture, the obtained tissue culture seedlings are planted on a prepared seedling hardening substrate, and the seedlings are transplanted to a large filed for plantation after seedling hardening, wherein the seed germination culture medium comprises MS (magnesium sulfate), 30g / L of sugar, 8g / L of agar and 10g / L-12g / L of potatoes; the multiplication and subculture medium comprises MS, 1.0 mg / L of KT (kinetin), 0.2 mg / L of NAA (naphthalene acetic acid), 30g / L of sugar and 9g / L of agar; the rooting culture medium comprises MS, 0.2 mg / L of NAA, 1.0mg / L of IAA (indole acetic acid), 30g / L of sugar, 9g / L of agar and 25-30 g / L of potatoes; the seedling hardening substrate comprises medium moor peat soil, perlite and wood dust in the volume ratio being 5:2:3. The technology is mature, a system is perfect, the problems of low natural germination rate of bletilla striata and difficulty in obtaining of seedlings can be effectively solved, and the technology has better popularization and application value and broad market prospect.

The present invention discloses the imitating wild planting process of Chinese huperzine serrate. The process includes the following steps: 1. picking up wild Chinese huperzine serrate, cutting into 5-8 cm long cuttings, dipping the base parts of cuttings in 500-2000 ppm concentration naththylacetic acid solution with or without indole butyric acid or indole butyric acid and rootone for 10-20 sec, and planting in matrix comprising river sand and humus in the same weight ratio; and 2. planting the seedling of Chinese huperzine serrate in bamboo forest or other shaded environment with environment illumination intensity of 1000-3000 Lux and relative humidity not lower than 80 % in summer for Chinese huperzine serrate to grow. The present invention makes it possible to provide regenerated resource of Chinese huperzine serrate.

The invention provides a compound auxin for inducing plants to form aquatic roots and a method for forming aquatic roots, which consists of the following components in proportions by weight: 0.5-2 parts of indole butyric acid, 0.5-2 parts of indole acetic acid, 0.25-1 part of naphthaleneacetic acid and 1-4 parts of potassium dihydrogen phosphate. In the present invention, by selecting indole butyric acid, indole acetic acid and naphthalene acetic acid in an appropriate ratio, and adding the nutrient potassium dihydrogen phosphate, the training time of water rooting can be shortened, and the probability of inducing plants to form water rooting can be increased. And the formed aquatic root system has high vitality and developed root system.

The invention discloses a mixed rooting agent suitable for various plants. The mixed rooting agent is prepared from the following components in parts by weight: 18-23 parts of triacontanol, 90-108 parts ofboric acid (HBO3), 170-230 parts of naphthalene acetic acid(NAA), 18-23 parts of 6-benzyladenine(6-BA), 750-860 parts ofindole-3-butytric acid(IBA), 17-25 parts ofmanganese sulfate(MnSO4), 17-25 parts ofmagnesium sulfate (MgSO4), 7-15 parts of glycine and 8-13 parts of glutamic acid.As containing the amino acid components, the mixed rooting agent has the effects of providing nutrients and performing physiological regulation, is capable of improving the adversity ability of crops, and is beneficial to crop growth. With a growth hormone formula, the vigor of root systems can be improved, the growth of root systems and fibril can be promoted, and the rate of rooting of cuttings can be enhanced. By virtue of the mixed rooting agent, the content of chlorophyll of transplanted plants can be increased, and the photosynthesis of plants can be strengthened. By virtue of synergism of various hormones and amino acids, the growth of plants is promoted greatly, root hair of plants can be protected effectively, the adaptation of transplanted plants can be prolonged, the rooting speed of transplanted plants can be increased, and the growth of plants in the early state of transplanting can be enhanced.

A Podocarpus macrophyllus cuttage breeding method comprises the following steps: 1, selecting cuttings: selecting 10-15cm semi lignification Podocarpus macrophyllus branches with heels, removing blades on the lower portion of the branches, and keeping the upper 2 / 3 blades; 2, cutting treatment: using a knife to trim and smooth pedicles of the selected Podocarpus macrophyllus branches, immersing the lower 1-2cm of the branch into a naphthalene acetic acid auxin solution with concentration of 300mg / l, or spraying auxin on the surface of a seedbed at the rate of 500ml / square meter; 3, seedbed selection and processing; 4, drill holes; 5, cuttage; 6 post-cuttage management; 7, transplanting; 8 trimming moulding. The Podocarpus macrophyllus cuttage breeding method uses semi lignification branches with heels, so rooting is easier; the cuttings are disinfected and processed by auxin, and fertilization, weeding and insect control are timely carried out, thus improving Podocarpus macrophyllus cuttage survival rate.

An in-vitro culture method for catalpa bungei comprises 1) inducing callus, concretely, putting catalpa bungei on a callus induction medium for inducing callus, wherein the callus induction medium is obtained by adding 6-benzyladenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) on the basis of 1 / 2 MS basal medium; 2) propagating callus, concretely putting callus on a propagation medium for propagation, wherein the propagation medium is obtained by adding 6-benzyladenine (6-BA) and alpha-naphthalene acetic acid (NAA) on the basis of 1 / 2 MS basal medium; 3) inducing embryonic callus and differentiating somatic embryo, concretely putting propagated callus on an induction differentiation medium, wherein the induction differentiation medium is obtained by adding 6-benzyladenine (6-BA) and alpha-naphthalene acetic acid (NAA) on the basis of 1 / 2 MS basal medium; and 4) regenerating a plant, concretely putting somatic embryo obtained through differentiation on a regeneration medium for inducing somatic embryo to germinate, so as to obtain a catalpa bungei regeneration plant. The method aims at expanding catalpa bungei propagation coefficient and providing conditions for germplasm preservation and genetic transformation.

The invention discloses a method for cutting propagation of gardenias through plugging shallow water bathing. The method includes the steps of pruning cuttings, disinfecting the cuttings with carbendazim, treating the cuttings with alpha-naphthalene acetic acid, disinfecting a substrate with formaldehyde, immersing the cuttings and cutting, transplanting the cuttings to a shallow water pond, and spraying liquid urea, wherein the temperature of a bed surface is not higher than 32 DEG C, and the substrate is formed by rice field soil and mushroom cultivation waste in a mixed mode according to the mass ratio of 7:3. The method has the advantages that a soilless culture technique is combined with a hormone treatment method, gardenia plugging shallow water bathing rooting is carried out, the rooting speed is high, roots are not damaged in transplanting, measures of seed removal, watering and spraying in a garden field are eliminated, the method is simple and feasible, the problem that a traditional cutting rooting plant of a gardenia is difficult to survive is solved, by means of the method, large-scale and industrialized production of the gardenias can be achieved in a plugging shallow water bathing mode, ornamental value of the gardenias is improved, large quantities of seedlings can be provided for traditional gardenia production, and economic efficiency, ecological efficiency and social efficiency are obvious.

The invention discloses a culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes, which belongs to the field of agricultural biotechnologies. The culture medium comprises the following compositions: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, disodium ethylenediamine tetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extracts, sucrose, 6-benzylaminopurine, naphthalene acetic acid, kinetin, zeatin and heteroauxin beilining. The culture medium is applied to the induction and culturing of double-haploid stem regenerated seedlings of potatoes, the test effect is good, the initiating rate is increased by 76%, and the seedling rate is as high as 80.5%. The regeneration rate of double-haploid stems of potatoes is significantly increased, thereby laying a foundation for the establishment of a genetic transformation system; and the culturing mode adopts a one-step seedling method, so that the pollution probability is reduced.

The invention relates to a novel fruit expander for pears and a use method thereof, and the novel fruit expander can be used for overcoming the defects of high labour of traditionally applying the expander and multiple side effects. The novel fruit expander is formed by scientifically proportioning compound sodium nitrophenolate, gibberellin, pimacol, additives, auxiliaries and a plurality of nutrient elements. The action mechanisms of the novel fruit expander comprise promoting new roots, and increasing absorption area and leaves to increase photosynthetic efficiency, promoting the general circulation of the nutrient substances of tree bodies, and the microcirculation of nutrient substances among cells and in cells, exciting cell activity, enhancing cell division and expansion, improving cell wall permeability, promoting protoplasmic streaming, urging photosynthetic products to be accumulated in fruits, and ensuring robust tree bodies, high yield, early harvest, large fruit size and high sugar content of pear trees. Moreover, the novel fruit expander is free from any side effect and low in cost, and can be easily accepted by pear farmers.

The invention discloses a rooting induction method of a test-tube plantlet of cunninghamia lanceolata. The rooting induction method comprises the steps of: cutting a 1-2cm multiplication subculture plantlet, inoculating in a pre-rooting culture medium for culturing, wherein the pre-rooting culture medium uses 1 / 2MS-MS as a base culture medium, and also comprises 0.02-0.08mg / L of NAA (Naphthalene Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; carrying out rooting culture after pre-rooting and culturing for 15-25 days, wherein a rooting culture medium uses improved 1 / 2 MS as a basic culture medium, and also comprises 0.10-0.25mg / L, 0.05-0.08mg / L of IAA (Indole-3-Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; and placing in a proper environment for culturing to obtain a rooted test-tube plantlet. After the cunninghamia lanceolata is rooted and cultured by adopting the rooting induction method, the rooting rate, the root system quantity and the root system germination uniformity of the test-tube plantlet of the cunninghamia lanceolata can be increased, more excellent rooted test-tube plantlets are provided for production and application, and good economic benefit and social benefit are obtained.

The invention belongs to the field of plant biotechnologies and particularly discloses a culture medium and culture method for promoting the growth of regeneration buds of echinacea purpurea. According to the culture medium, DA-6 (diethyl aminoethyl hexanoate) of corresponding concentration is particularly added to an MS formula in accordance with that the regeneration buds of different ploidies have different sensitivities to DA-6, besides adding 15-60g / L of saccharose, 3-9g / L of agar and 0.01-0.2mg / L of NAA (naphthalene acetic acid). The culture medium has the advantage that the height, weight, the weight, diameter and number of roots, the length of primary roots, total root length, root cap ratio and transplanting survival rate of the regeneration buds all can be increased remarkably. By applying the culture medium and the culture method, the development of the scientific research work related to the biotechnological breeding of echinacea purpurea can be promoted.

The invention discloses a dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and an application of the dendrobium nutrient solution formula. A nutrient solution comprises the following components by volume percentage: 70-75% of Knudson C culture medium macroelements, 8-15% of microelements, 0.75-1.25% of 200mg / ml growth hormone NAA (naphthalene acetic acid) and 15-20% of carbendazim antibacterial agent with a mass fraction of 0.6-0.7%. Root-soaking treatment is conducted on the test-tube plantlets before transplanting, water is sprayed for once every day after the test-tube plantlets are transplanted, and the dendrobium nutrient solution is sprayed for once every week, so that the fast growth and the high quality of the cultivating plantlets are facilitated. The two problems of low transplanting survival rate and slow growth of the test-tube plantlets are solved, therefore, not only is the transplanting survival rate of the dendrobium test-tube plantlets increased, but also the fast growth, high yield, stable yield and high quality of the dendrobium cultivating plantlets are facilitated; and a technical support is provided for establishing an artificial large-area dendrobium cultivating base and achieving industrial dendrobium production.

The invention relates to a cutting propagation method of a zelkova tree. The cutting propagation method of the zelkova tree comprises regulations of choosing cutting slips, choosing cutting substrate, choosing root-promoting hormone and the like. Cutting time, the cutting substrate, the cutting hormone and age of a mother plant of cutting branches which are most suitable for growth of the zelkova tree are screened out. Root-promoting rate of the cutting propagation of the zelkova tree can be greatly improved by choosing 8-12 centimeters branches of the annual zelkova tree as the cutting slips, using a mixture of perlite and peat soil in a proportion of 3:1 as the substrate, using indolebutyric acid (IBA) and naphthalene acetic acid (NAA) with a concentration of 200 milligrams per liter to soak for three hours, and using ABT 1 root-promoting powder to soak for 4.5 hours. The cutting propagation method of the zelkova tree is convenient to operate without adding professional equipment, and is capable of effectively improving surviving rate of the cutting propagation of the zelkova tree.

The invention relates to a cultivating method of rose nutrient solution. The cultivating method includes A, planting, selecting plants, cutting all the fiber, reserving 3-5mm main roots, using carbendazim to immerse and disinfect, and immersing in naphtylacetic acid aqueous solution; B, planting in field, planting the plants with roots processed into a planting basket with 5-10mm depth and through holes; C, catalyzing roots, transferring the plants with the planting basket into perlite matrix, and controlling environment conditions via an automatic controlling system to catalyze new roots of the plants;D, accelerating roots, immersing into a nutrient solution water groove, accelerating the top of the roots of the plants generating new aquatic roots capable of suiting water via the automatic controlling system to control environment conditions so as to become commercial plants. The cultivating method has the advantages that 1, cultivating time is greatly reduced, 2, the plants are easy to be controlled by intelligent systems, so that factor-scale production can be realized and 3, plant diseases and insect pests are few and survival rate is greatly increased.

The invention discloses a method for performing cuttage propagation on Torreya yunnanenssi Cheng et L.K.Fu, belongs to the technical field of plant cultivation, and in particular relates to a method for performing cuttage propagation on Torreya yunnanenssi Cheng et L.K.Fu. The method is characterized by comprising the following steps of: (1) preparing a cutting wood: taking an annual-grown strong branch of which the length is between 8 and 12cm as the cutting wood; (2) putting the cutting wood into mixed liquid of naphthalene acetic acid aqueous solution of 1,000mg / L and chlorothalonil of 2,000mg / L for soaking; (3) paving a brick seedbed with the height of 20cm in a greenhouse tunnel, and paving a sand bed or a matrix bed on the brick seedbed; (4) inserting the cutting wood into the sand bed or the matrix bed; (5) carrying out daily management; and (6) exercising seedling and changing a garden: cultivating a cutting wood seedling root in the open air by changing the small greenhouse tunnel after the cutting wood seedling root grows out of the soil. With the adoption of the method, the problem that the Torreya yunnanenssi Cheng et L.K.Fu difficultly takes root is solved, and the method is simple and feasible and low in cost, so that the blank for performing cuttage propagation on Torreya yunnanenssi Cheng et L.K.Fu for culturing seedlings in a scale mode can be made up.

The invention discloses a method for rapidly breeding potted carnation through tissue culture. The method comprises the following steps of: cutting an explant and sterilizing; carrying out primary culture, wherein a primary culture medium comprises MS (Murashige and Skoog), 1.5mg / L of 6-BA (6-Benzylaminopurine), 0.2mg / L of NAA (Naphthalene Acetic Acid), 30g / L of sucrose and 8g / L of agar; carrying out subculture, wherein a subculture medium comprises MS, 0.8mg / L of 6-BA, 0.2mg / L of NAA, 30g / L of sucrose and 8g / L of agar; carrying out rooting culture, wherein a rooting culture medium comprises MS, 0.2mg / L of NAA, 0.1mg / L of IAA(Indole Acetic Acid), 30g / L of sucrose and 8g / L of agar; and washing the culture mediums away and then holding the temperature and humidity for seedling hardening and transplanting. The method is simple and easy to operate, simple in procedure and high in working efficiency, and is capable of effectively holding excellent properties of parents. According to the method, the differentiation rate of a tissue culture seedling is increased. The method has the characteristics of high multiplication speed, high rooting rate, high survival rate and low cost.

The invention discloses an acer negundo aurea tissue culture rapid propagation method which belongs to the technical field of plant tissue culture, and includes explant sterilization, starting culture, primary culture, subculture and transplanting steps. Only one medium is used in the method, and the medium is 1 / 2DKW+0.1mg / L NAA (naphthalene acetic acid) +0.05mg / L IAA (indole acetic acid) + 30g / L sucrose + 5g / L agar, proliferation and rooting culture can be performed on the same medium, and the method has the characteristics of being simple in culture process, easy to operate, high in propagation coefficient, short in breeding cycle, free of hardening, and high in survival rate.

The invention discloses a tobacco seed primer, which relates to the technical field of crop seeds. The tobacco seed primer is prepared by dissolving gibberellin, naphthalene acetic acid and indoleacetic acid in water, has the characteristics of obviously strengthening the self-stress resistance and the germination advantage of tobacco seeds, improving the uniformity of seedling emergence, shortening seedling emergence time, promoting the obvious improvement of the quality of tobacco seedlings and so on, is applicable to the priming treatment of tobacco seeds in tobacco planting areas across the country before the coating, and is the tobacco seed primer with better priming effect.

A culturing medium antiseptic for Anoectochilus roxburghii (Wall.) Lindl tissue culture comprises an MS culturing medium mother liquor or a B5 culturing medium mother liquor, and each 1000ml of the culturing medium antiseptic also comprises 1-5g of chitosan, 1-4g of tea polyphenol, 80-120g of mashed banana, 15-30g of sucrose, 2-4g of peptone, 0.2-0.5mg of indole 3-butyric acid potassium, 0.2-0.6mg of pimacol and 0.5-1mg of 6-benzylaminopurine. The pH value of the culturing medium antiseptic is adjusted to 5.4-5.7 by hydrochloric acid or sodium hydroxide. The culturing medium antiseptic adopts chitosan and tea polyphenol, and the chitosan and tea polyphenol have good bacteriostasis and sterilization effects, are health preserving competitive products beneficial for the health, can effectively reduce the pesticide residues, and can promote the healthy growth of Anoectochilus roxburghii (Wall.) Lindl. The mashed banana and peptone are rich in organic potassium and organic nitrogen, can effectively meet the nutrient demands of Anoectochilus roxburghii (Wall.) Lindl, and can also improve the content of effective components in Anoectochilus roxburghii (Wall.) Lindl in order to ensure the drug effect.

The invention discloses a mass pteridophyta reproduction method. The mass pteridophyta reproduction method includes the steps of (1), harvesting pteridophyta spores; (2), sowing, namely, mixing collected spores with a hormone solution, spraying the mixture into a sowing medium, and reproducing by keeping relative air humidity above 85% and temperature ranging from 25 DEG C to 27 DEG C, wherein the hormone solution consists of 1 / 2 MS (Murashige and Skoog) culture medium, NAA (naphthalene acetic acid) and GA3; (3), performing 'two-time transplant', namely, transplanting gametocytes on the culture medium after the gametophytes mature and before sporophytes arise, and transplanting seedlings into yellow soil when the sporophytes have 2-3 leaves. By the aid of the mass pteridophyta reproduction method, spore germination rate can be increased, and prothallus development can be promoted; production sowing quantity is guided effectively, and sporophyte growth and transplant management are benefitted; gametophyte fertilization is further benefited by 'two-time transplant', and seedling growth uniformity can be improved while seedling survival rate can be increased.

The invention discloses a compound pesticide for preventing and controlling liberobacter asiaticum and a preparation method thereof. According to objective requirements of prevention and control of liberobacter asiaticum, ZhongShengmycin and oxytetracycline hydrochloride are used as sterilization pesticides, salicylic acid is used as an anti-disease regulation agent and alpha-naphthalene acetic acid is used as a rooting accelerant; the materials are reasonably blended, and pesticide application is carried out by adopting a trunk infusion method, so that the advantages of the agents are complementary to each other; and the compound pesticide has the properties of high efficiency, low toxin, safety, economy and multiple functions, and can be used for preventing and controlling liberobacter asiaticum very well.

The invention relates to a fruit thinning medicament for a pear tree. The medicament comprises the following components in parts by weight: 6-9 parts of pimacol, 6-9 parts of ethephon, 2-5 parts of potash fertilizers and 1-3 parts of phosphorus fertilizers. A medicament fruit thinning method applied to the pear tree by utilizing the medicament comprises the following steps of: (1) uniformly mixing the components in parts by weight according to the claim 1 with water with the 1000-time weight of the components, so as to prepare the fruit thinning medicament; (2) spraying the medicament prepared in the step (1) to the surfaces of leaves of the pear tree after 15-20 days of a flower drop period of the pear tree, wherein the medicament is sprayed in each 3-5 days and is continuously sprayed for three times; and (3) carrying out manual thinning supplementation by utilizing a fruit thinning shear after medicament fruit thinning is finished for 10-20 days, and leaving a fruit in each inflorescence. Compared with a manual fruit thinning process, the medicament fruit thinning method has the advantages that the speed is fast, the wasting of human and material resources is reduced, and fruits are immediately tinned, so that the yield and the quality of the fruits are guaranteed.

The invention relates to a cultivation and separation method for taxus stem cells. The method comprises the steps of sterilizing annual epicormic branches of taxus cuspidate, with the diameter of 1 centimeter, for 10 minutes by using 0.1% mercury chloride; cutting off explants including periderm, phloem and cambium from the epicormic branches, cutting into slices; putting the slices on a B5 medium containing 0.2-2.0 mg.L<-1> picloram and 0.2-1.5 mg.L<-1> naphthalene acetic acid, culturing in the dark at a temperature of 25 DEG C; taking the explants with obvious cambium proliferation out after two weeks; separating cambium stem cells and transferring the stem cells to a subculture medium for culture, wherein the subculture medium is a B5 medium containing 0.2-2.0 mg.L<-1> picloram and 0.2-1.5 mg.L<-1> naphthalene acetic acid; and sub-culturing one time every two weeks. A mass of stem cells can be obtained in a short time. The stem cells cultured by the method are cells in an undifferentiated state which have the capacity of infinite division and can obtain a mass of the stem cells, so that a great amount of paclitaxel can be produced, thereby meeting the needs of patients.

The present invention relates to plant biological engineering and biological technology, and is fast reproduction of Maka seedling. The reproduction of Maka seedling includes the steps of: obtainingexplant; inducing callus; differentiating adventitious bud; rooting culture; post-rooting treatment; and transplanting after hardening seedling. MS or improved MS culture medium is adopted and in different culture stages, 6-benzyl aminopurine (6BA) and naphthalene acetic acid (NAA) in different concentration and compounding ratio are used. The present invention makes it possible to provide highquality test tube seedlings for large-scale plantation of Maka as a kind of important economic crop.

The invention discloses a method for synchronously preventing and controlling fruit cracking and a fruit shrinking disease of ziziphus jujuba mill, namely, key mineral element supplementation, chemical prevention and growth regulator application are combined. The method is characterized in that soil fertilization is carried out by mixing potassium sulfate and manganese sulfate according to a certain proportion, and sodium silicate and calcium chloride are sprayed on leaves, so that key mineral elements and beneficial elements can be effectively supplemented, nutrient balance can be regulated, and the resistance is increased; 6-BA (Benzyladenine) is sprayed on the leaves in an early stage, so that pericarpic cell division is stimulated, and elasticity of jujube peel is enhanced; NAA (Naphthalene Acetic Acid) is sprayed in a later stage, so that maturing of jujube fruits is delayed, and a cloudy and rainy period in the early of mid September is avoided; mancozeb is sprayed on the leaves, so that pathogenic bacteria on body surface can be effectively killed, a layer of dense protective medicine membrane is formed on the surface of crop, and germination and invasion of the pathogenic bacteria are inhibited. According to the method disclosed by the invention, the morbidity of the fruit cracking and the fruit shrinking disease of the ziziphus jujuba mill can be synchronously and obviously reduced, and a total diseased fruit rate can be reduced from 80 to 100 percent to 20 percent or less. A technology is applied to prevention and control of the fruit cracking and the fruit shrinking disease of the ziziphus jujuba mill, the method is simple, the cost is low, the prevention and control efficiency is high, and the effect is lasting.

The invention provides a cut-flower chrysanthemum morifolium seedling breeding and cultivating method. The cut-flower chrysanthemum morifolium seedling breeding and cultivating method includes steps of root cutting, breeding, planting and growth management, and is characterized in that during root cutting, adopting a scion stem long in 10cm, disposing the entire scion stem in the following rooting liquids including distilled water+0.4-0.6mg / L of IBA (indole butyric acid)+0.4-0.6mg / L of NAA (naphthalene acetic acid)+3-4ml / L of azoxystrobin+2-3ml / L of gold ridomil, and cutting after soaking for 10-15 minutes. The scion stem which is long is easy to root, root distribution is uniform and strong, bacteria carried with the stem are effectively killed, cuttage diseases are reduced, death rate at the seedling stage is decreased, cut-flower quality is kept constant, the time from cuttage seedling planting to cut-flower harvesting lasts 70 days, and five batches of the cut-flower chrysanthemum morifolium can be continuously produced in a year. Besides, rot of the seedlings is reduced, death rate is decreased to 2-3%, and plants are strong and healthy, so that yield of the cut-flower is increased to 75-90%.

The invention discloses a proliferation medium for Sierra cowberry blueberry tissue culture. 1L of the proliferation medium comprises modified WPM (woody plant medium), 0.5mg of NAA (Naphthalene Acetic Acid), 0.2mg of GA, 2-3mg of zeatin (ZT), 30g of saccharose, and 7g of agar, and has pH value of 5.2. 1L of the modified WPM contains macroelements, iron salts, microelements and organic substances, wherein the macroelements comprise 893mg of potassium nitrate, 119mg of ammonium sulfate, 370mg of magnesium sulfate, 278mg of calcium nitrate tetrahydrate, and 170mg of monopotassium phosphate; the ferric salts comprise 74.6mg of ethylene diamine tetraacetic acid, and 55.6mg of ferrous sulfate; the microelements comprise 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate, 6.2mg of boric acid, 0.415mg of potassium iodide; and the organic substances comprise 0.5mg of niacin, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. The proliferation rate can reach more than 12 after the medium is used for 30 days.