Mass pteridophyta reproduction method

Abstract

The invention discloses a mass pteridophyta reproduction method. The mass pteridophyta reproduction method includes the steps of (1), harvesting pteridophyta spores; (2), sowing, namely, mixing collected spores with a hormone solution, spraying the mixture into a sowing medium, and reproducing by keeping relative air humidity above 85% and temperature ranging from 25 DEG C to 27 DEG C, wherein the hormone solution consists of 1 / 2 MS (Murashige and Skoog) culture medium, NAA (naphthalene acetic acid) and GA3; (3), performing 'two-time transplant', namely, transplanting gametocytes on the culture medium after the gametophytes mature and before sporophytes arise, and transplanting seedlings into yellow soil when the sporophytes have 2-3 leaves. By the aid of the mass pteridophyta reproduction method, spore germination rate can be increased, and prothallus development can be promoted; production sowing quantity is guided effectively, and sporophyte growth and transplant management are benefitted; gametophyte fertilization is further benefited by 'two-time transplant', and seedling growth uniformity can be improved while seedling survival rate can be increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a fern scale propagation method. Background technique [0002] Ferns, also known as ferns, are a large group of plants between bryophytes and seed plants in the plant evolution system. China is rich in fern resources, about 2,600 species, accounting for 1 / 4 of the world's total fern resources, widely distributed throughout the country, mostly in South China and Southwest China, and there are wild or cultivated ornamental ferns in Guangdong Province alone. 277 species. A small number of ferns have been used as ornamental plants earlier, such as Cuiyuncao, but most ferns have not been effectively developed and utilized. related to cost. Most people think that ferns grow in a damp environment, and are delicate and difficult to maintain; the life history of ferns is even more special, and the growth characteristics of no flowers and no fruits are different from those of seed plants. ...

AI Technical Summary

Problems solved by technology

The production of domestic ferns mainly relies on imported seedlings for processing or reproduction; very few rare species are propagated in large quantities through tissue culture, but the cost is high and the technology is difficult to apply, resulting in most of them having ornamental Value ferns are not available for generalization

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