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Culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes

A double haploid and regenerated plant technology, applied in the field of agricultural biology, can solve the problems of weak productivity and viability, and achieve the effect of high seedling rate

Inactive Publication Date: 2013-01-16
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because potato double haploids are different from common cultivars, their productivity and viability are relatively weak; in addition, for subsequent gene transformation and functional verification, stem segments were used as explants in this experiment, so this medium is not suitable for potato Double Haploid Stem Segments Induced to Regenerate Plants in Vitro

Method used

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  • Culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes
  • Culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes
  • Culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A medium for in vitro induction of plant regeneration from potato double haploid stems:

[0056] (1) Inorganic substances: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, potassium dihydrogen phosphate 170mg / L, magnesium sulfate 370mg / L, calcium chloride 440mg / L, potassium iodide 0.83mg / L, boric acid 6.0mg / L , Manganese sulfate 16.9mg / L, zinc sulfate 9.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, ferrous sulfate 27.8mg / L, ethylenediamine tetra Disodium acetate 37.3mg / L;

[0057] (2) Organic matter: inositol 100mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 2mg / L;

[0058] (3) Phytohormones: 6-benzylamino adenine 2mg / L, naphthaleneacetic acid 0.01mg / L, kinetin 2mg / L, indole acetic acid 2mg / L, zeatin 4mg / L;

[0059] (4) Sucrose 30g / L;

[0060] (5) Baili Ning 4g / L.

[0061] Preparation method: Accurately weigh each component according to the formula, add deionized water to fully dissol...

Embodiment 2

[0064] A medium for in vitro induction of plant regeneration from potato double haploid stems:

[0065] (1) Inorganic substances: potassium nitrate 1900mg / L, ammonium nitrate 1600mg / L, potassium dihydrogen phosphate 175mg / L, magnesium sulfate 360mg / L, calcium chloride 430mg / L, potassium iodide 0.83mg / L, boric acid 6.0mg / L , Manganese sulfate 16.9mg / L, zinc sulfate 9.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, ferrous sulfate 27.8mg / L, ethylenediamine tetra Disodium acetate 37.3mg / L;

[0066] (2) Organic matter: inositol 100mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 1mg / L;

[0067] (3) Plant hormones: 6-benzylamino adenine 2mg / L, naphthalene acetic acid 0.05mg / L, indole acetic acid 0.5mg / L, zeatin 4mg / L;

[0068] (4) Sucrose 30g / L;

[0069] (5) Baili Ning 4g / L.

[0070] Configuration method: same as Example 1.

Embodiment 3

[0072] A medium for in vitro induction of plant regeneration from potato double haploid stems:

[0073] (1) Inorganic substances: potassium nitrate 1900mg / L, ammonium nitrate 1600mg / L, potassium dihydrogen phosphate 165mg / L, magnesium sulfate 370mg / L, calcium chloride 440mg / L, potassium iodide 0.83mg / L, boric acid 6.0mg / L , Manganese sulfate 16.9mg / L, zinc sulfate 9.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, ferrous sulfate 27.8mg / L, ethylenediamine tetra Disodium acetate 37.3mg / L;

[0074] (2) Organic matter: hydrolyzed casein 100mg / L, inositol 100mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, thiamine hydrochloride 0.1mg / L, glycine 2mg / L;

[0075] (3) Phytohormones: 6-benzylamino adenine 3mg / L, kinetin 1mg / L, indole acetic acid 2mg / L, zeatin 1mg / , naphthalene acetic acid 0.4mg / L;

[0076] (4) Sucrose 30g / L;

[0077] (5) Baili Ning 4g / L.

[0078] Configuration method: same as Example 1.

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Abstract

The invention discloses a culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes, which belongs to the field of agricultural biotechnologies. The culture medium comprises the following compositions: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, disodium ethylenediamine tetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extracts, sucrose, 6-benzylaminopurine, naphthalene acetic acid, kinetin, zeatin and heteroauxin beilining. The culture medium is applied to the induction and culturing of double-haploid stem regenerated seedlings of potatoes, the test effect is good, the initiating rate is increased by 76%, and the seedling rate is as high as 80.5%. The regeneration rate of double-haploid stems of potatoes is significantly increased, thereby laying a foundation for the establishment of a genetic transformation system; and the culturing mode adopts a one-step seedling method, so that the pollution probability is reduced.

Description

Technical field [0001] The invention relates to a solid culture medium used for in vitro induction of regeneration plants by potato double haploid stems, and belongs to the field of agricultural biotechnology. Background technique [0002] Common potato cultivars are autotetraploid, and its haploid is also called double haploid. There are two main ways for tetraploid potatoes to produce double haploids: one is to use anther culture to artificially induce monomale reproduction, and the other is to induce parthenogenesis through diploid pollinators. [0003] Common potato cultivars belong to tetraploid, with an unusually complex genetic background, while potato is double haploid, with a high degree of gene purity, and the interaction between sites is reduced, which simplifies the genetic ratio and facilitates our study of the function and distribution of gene sequences . Especially in recent years, with the completion of potato genome sequencing, we can use double haploids for more...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘芳杨元军王培伦马伟清董道峰陈广侠马蕾
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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