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Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata

A rapid propagation and high-efficiency technology of tissue culture, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of poor natural reproduction and low seed germination rate, and achieve easy composition, reasonable ratio, The effect of reducing the cost of cultivation

Active Publication Date: 2015-12-16
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a rapid multiplication technique for tissue culture of A. radiata seedlings, aiming at the problems of low seed germination rate and poor natural reproduction in the current artificial cultivation of A. radiata. It can not only efficiently obtain tissue cultured seedlings of A. The hardening and domestication mechanism of tissue culture seedlings has been studied. Using tissue culture technology, a large number of seedlings can be obtained, laying the foundation for the large-scale planting of Baiji

Method used

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  • Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata
  • Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata
  • Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata

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Embodiment 1

[0024] Embodiment 1: A kind of high-efficiency tissue culture and rapid propagation technology of Radix Rhizoma Rhizoma Rhizoma, its steps include material preparation, seed aseptic germination, multiplication and subculture, rooting culture, hardening and domestication, and the specific methods are as follows:

[0025] 1. Material preparation

[0026] Select mature but uncracked white and pods, first wash the surface impurities with running water and drain, then wipe the surface of the pods with 75% alcohol cotton balls, then transfer them to the ultra-clean workbench, clean them with 75% alcohol After soaking in the solution for 30 seconds, sterilize with 0.1% mercuric chloride solution for 6 minutes and rinse with sterile water for 2-3 times, and then dry the surface moisture with sterile filter paper to obtain sterile white and pomegranate capsules.

[0027] 2. Aseptic germination of seeds

[0028] 2.1. Preparation of seed germination medium

[0029] Add 30g / L of sucrose...

Embodiment 2

[0047] Embodiment 2: A kind of high-efficiency tissue culture and rapid propagation technology of Radix Rhizoma Rhizoma Rhizoma, its steps include material preparation, seed aseptic germination, multiplication and subculture, rooting culture, hardening and domestication, and the specific methods are as follows:

[0048] 1. Material preparation

[0049] Select mature but uncracked white and pods, first wash the surface impurities with running water and drain, then wipe the surface of the pods with 75% alcohol cotton balls, then transfer them to the ultra-clean workbench, clean them with 75% alcohol After soaking in the solution for 30 seconds, sterilize with 0.5% mercuric chloride solution for 6 minutes and rinse with sterile water for 2-3 times, and then dry the surface moisture with sterile filter paper to obtain sterile white and pomegranate capsules.

[0050] 2. Aseptic germination of seeds

[0051] 2.1. Preparation of seed germination medium

[0052] Add 30g / L of sucrose...

Embodiment 3

[0070] Embodiment 3: A kind of high-efficiency tissue culture and rapid propagation technology of Radix Rhizoma Rhizoma Rhizoma, its steps include material preparation, seed aseptic germination, multiplication and subculture, rooting culture, hardening and domestication, and the specific methods are as follows:

[0071] 1. Material preparation

[0072] Select mature but uncracked white and pods, first wash the surface impurities with running water and drain, then wipe the surface of the pods with 75% alcohol cotton balls, then transfer them to the ultra-clean workbench, clean them with 75% alcohol After soaking in the solution for 30 seconds, sterilize with 0.3% mercuric acid solution for 6 minutes and rinse with sterile water for 2-3 times, and then use sterile filter paper to blot the surface moisture to obtain sterile capsules.

[0073] 2. Aseptic germination of seeds

[0074] 2.1. Preparation of seed germination medium

[0075] Add 30g / L of sucrose, 8g / L of agar and 10g / ...

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Abstract

The invention discloses an efficient tissue culture and rapid propagation technology for seedlings of bletilla striata. According to the technology, bletilla striata seeds are sown on a seed germination culture medium for sterile germination, the obtained seedlings are inoculated to a multiplication and subculture medium one by one for multiplication and subculture, clustering seedlings are obtained and inoculated to a rooting culture medium for rooting culture, the obtained tissue culture seedlings are planted on a prepared seedling hardening substrate, and the seedlings are transplanted to a large filed for plantation after seedling hardening, wherein the seed germination culture medium comprises MS (magnesium sulfate), 30g / L of sugar, 8g / L of agar and 10g / L-12g / L of potatoes; the multiplication and subculture medium comprises MS, 1.0 mg / L of KT (kinetin), 0.2 mg / L of NAA (naphthalene acetic acid), 30g / L of sugar and 9g / L of agar; the rooting culture medium comprises MS, 0.2 mg / L of NAA, 1.0mg / L of IAA (indole acetic acid), 30g / L of sugar, 9g / L of agar and 25-30 g / L of potatoes; the seedling hardening substrate comprises medium moor peat soil, perlite and wood dust in the volume ratio being 5:2:3. The technology is mature, a system is perfect, the problems of low natural germination rate of bletilla striata and difficulty in obtaining of seedlings can be effectively solved, and the technology has better popularization and application value and broad market prospect.

Description

technical field [0001] The invention belongs to the technical field of using tissue culture to raise seedlings of medicinal plants, and in particular relates to a high-efficiency tissue culture and rapid propagation technology of bletilla seedlings. Background technique [0002] White and Bletilla striata It is a perennial herb belonging to Orchidaceae. It is a commonly used medicinal material. Its tubers are used as medicine. It has the effects of astringent, hemostasis, swelling and granulation. It is used to treat hemoptysis, hematemesis, traumatic bleeding, sore swelling, and chapped skin. [0003] Because the white and wild resources are mainly relied on in the market, the blind and private mining and indiscriminate digging lead to the depletion of white and wild resources. The seeds of Baiji are very small, and there is no endosperm, so the natural germination rate is extremely low. Currently, the commonly used division propagation has a low reproduction coefficient a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 侯典云张改娜胥华伟贾小平史国安
Owner HENAN UNIV OF SCI & TECH
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