11 results about "Picloram" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction culture medium for embryogenic calluses of rubber tree variety thermal reclamation 628, a method for inducing somatic embryogenesis by using the induction culture medium and application of the induction culture medium. According to the induction culture medium, an improved MS culture medium is used as a basic culture medium, and the induction culture medium further comprises 0.1 g / L to 1 g / L of proline, 0.1 g / L to 1 g / L of arginine, 0.1 g / L to 1 g / L of glutamine, 10 mg / L to 70 mg / L of adenine sulfate, 1 mg / L to 10 mg / L of silver nitrate, 0.2 mg / L to 2 mg / L of 2, 4-D, 0.2 mg / L to 2 mg / L of Zetin and 0.2 mg / L to 2 mg / L of Picloram. According to the method, the somatic embryo occurrence rate can be remarkably increased by optimizing the induction culture medium of the embryogenic callus of the heat reclamation 628, the anther somatic embryo occurrence rate of the heat reclamation 628 of the rubber tree variety can be increased to 50% or above by utilizing the induction culture medium, and the cotyledon embryo occurrence rate can be increased to 20% or above.

The invention provides a fritillaria tissue culture medium, a preparation method, a fritillaria tissue culture method and application, and belongs to the technical field of plant tissue culture. It is found for the first time that low-concentration picloram can achieve efficient browning resistance of fritillary callus; compared with the prior art, the formula of the fritillaria tissue culture medium has the advantages that the browning resistance efficiency is remarkable, healthy proliferation of calluses is synchronously promoted, and the contradiction that'browning inhibition 'and'growth promotion' are difficult to consider at the same time in a traditional fritillaria tissue culture method is solved. The method is suitable for various fritillaria such as fritillaria unibiacteata, bulbus fritillariae cirrhosae and bulbus fritillariae thunbergii, and has universality and industrialization prospects.

The invention relates to a method for preparing 3, 4, 5, 6-tetrachloropicolinic acid from picloram solid residues. The method comprises the following steps: dissolving picloram solid residues which take 6-amino-3, 4, 5-trichloropicolinic acid and 4-amino-3, 5, 6-trichloropicolinic acid as main components as raw materials in 1, 4-dioxane or other solvents, adding concentrated nitric acid to carry out N-amino nitration, then adding thionyl chloride to carry out sealed heating chlorination, and carrying out post-treatment to obtain 3, 4, 5, 6-tetrachloropicolinic acid. According to the method, picloram solid residues are converted into production raw materials with practical application value, diazotization operation in a traditional method is avoided, and the method has the advantages of being safe and mild in reaction, easy to operate, green and economical.

The invention discloses a detection method of a picloram and triclopyr butoxyethyl ester compound herbicide, which comprises the following steps: S1, sample pretreatment: taking picloram to prepare a standard sample 1, taking triclopyr butoxyethyl ester to prepare a standard sample 2 for later use, and taking a sample to be detected to dilute for later use; s2, preparing a mobile phase: mixing an organic solvent and water to prepare the mobile phase; s3, the standard sample 1, the diluted to-be-detected sample and the standard sample 2 are injected into the high performance liquid chromatograph according to the sequence, and liquid chromatography detection is carried out by adopting a gradient elution program. According to the method, picloram and triclopyr butoxyethyl ester can be stably peaked, errors in calculation of the peak area proportion of the two components caused by too large or too small peak area are avoided, picloram and triclopyr butoxyethyl ester can be separated in a short time, and the detection efficiency is improved.

A method for preparing 5-fluoro-6-(bromo or chloro)picolinic acid analogs, or derivatives thereof, from picolinic acid, picolinic esters, or nitrile analogs of picolinic acid is provided. The method includes the following chemical process steps: (1) introducing a phthalyl group onto the 4-amino substituent of picolinic acid, picolinic esters, or nitrile analogs of picolinic acid, (2) adding two fluorine atoms at the 5,6-positions of the pyridine ring using a Halex fluorination chemical process, (3) removing the phthalyl group, hydrolyzing the ester or nitrile substituent, and adding chlorine or bromine at the 6-position by treatment with acid and water, and finally, (4) esterifying the 5-fluoro-6-(bromo or chloro)picolinic acid produced in step (3) to 5-fluoro-6-(bromo or chloro)picolinic esters.

The invention discloses an picloram triethanolamine salt water agent auxiliary agent and a preparation method thereof. The auxiliary agent is prepared from the following components in percentage by weight: 20-45% of dihydrogenated tallow dimethyl ammonium chloride, 10-20% of triethanolamine, 10-20% of sodium chloride, 5-10% of sodium chloride, 5-10% of sodium chloride and the balance of water. 15 to 30% of hydrogenated tallow amine acetate; 10 to 20% of hexadecyl dimethyl allyl ammonium chloride; 1-2% of dioctyl sodium sulfosuccinate; and the balance water. The picloram triethanolamine ammonium salt aqueous solution processed by the method is not easy to solidify at low temperature, has the characteristics of high efficiency, high environmental and biological safety, good preparation stability and the like, is particularly suitable for northern use, and has a good application prospect.

The invention discloses a gene gun mediated sugarcane genetic transformation method and a special culture medium thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to carry out sugarcane genetic transformation. The sugarcane genetic transformation method comprises the following steps: inducing sugarcane leaf sheaths or tillering plants on a callus induction culture medium to obtain calluses, and bombarding the calluses together with DNA to be transformed and regeneration factors to realize sugarcane genetic transformation, the callus induction culture medium contains 2 mg / L of 2, 4-D, 0.5 g / L of acid hydrolysis casein, 0.5 g / L of inositol, 1.2 mg / L of copper sulfate pentahydrate, 3.5 g / L of plant gel, 5 [mu] M of silver nitrate, 200 mg / L of ascorbic acid and 2 mg / L of picloram, and the pH value is 5.8; and the regeneration factor is TamGRF4-GIF1 or SomGRF4-GIF1, and the regeneration factor is TamGRF4-GIF1. The sugarcane genetic transformation method and the special culture medium thereof can be used for sugarcane genetic transformation.

The invention provides a weeding composition containing oxyfluorfen and picloram. The mass ratio of the oxyfluorfen to the picloram is (5: 1)-(1: 20). The composition can be prepared into an agriculturally suitable preparation for controlling weeds. The weeding composition disclosed by the invention has a remarkable synergistic effect, and can effectively reduce the use dosage of active ingredients, enlarge the weed control spectrum, inhibit the spread of alien invasive weed solidago canadensis, reduce environmental pollution and protect ecological diversity.

The invention discloses a fungicide and herbicide compound composition and application thereof. The compound composition comprises any one herbicide and any one fungicide. The herbicide is prepared from glyphosate, fluroxypyr, clopyralid, picloram, sulfometuron methyl, MCPA (2-methyl-4-chlorophenoxyacetic acid) and dicamba; the fungicide is prepared from mancozeb, tebuconazole and carbendazim. According to the invention, the fungicide and the herbicide are combined according to a specific ratio, so that a remarkable synergistic interaction effect is shown. During implementation, the fungicide is firstly applied to soil, then the herbicide is sprayed, the fresh weight inhibition rate of solidago canadensis can be increased to 90% or above, the regeneration inhibition rate exceeds 85%, and the dosage is reduced by 33%-50% compared with the dosage of a single herbicide. The compound composition can effectively overcome the drug resistance of target weeds and reduce the environmental load of chemical agents, has the advantages of lasting prevention and control effect, low application cost and safety to associated plants, and provides an innovative solution for treatment of invasive plants.

The invention discloses a herbicidal composition and its application, and relates to the field of pesticides. The composition comprises the following raw materials in percentage by weight: 5-60% of propanil, 1-40% of triclopyr, 0.1-22% of picloram, 3-15% of dispersant, 2-10% of wetting agent, 10-40% of disintegrant, and the rest of filler. The water-dispersible granules of the ternary composite composition of the invention can ultimately solve the problems of disintegration and suspension after heat storage by screening suitable dispersants, wetting agents and fillers. The prepared water-dispersible granules have the characteristics of fast disintegration, good dispersibility, high suspension rate and good stability, and are more conducive to the exertion of drug efficacy.