6 results about "Picloram" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction culture medium for embryogenic calluses of rubber tree variety thermal reclamation 628, a method for inducing somatic embryogenesis by using the induction culture medium and application of the induction culture medium. According to the induction culture medium, an improved MS culture medium is used as a basic culture medium, and the induction culture medium further comprises 0.1 g / L to 1 g / L of proline, 0.1 g / L to 1 g / L of arginine, 0.1 g / L to 1 g / L of glutamine, 10 mg / L to 70 mg / L of adenine sulfate, 1 mg / L to 10 mg / L of silver nitrate, 0.2 mg / L to 2 mg / L of 2, 4-D, 0.2 mg / L to 2 mg / L of Zetin and 0.2 mg / L to 2 mg / L of Picloram. According to the method, the somatic embryo occurrence rate can be remarkably increased by optimizing the induction culture medium of the embryogenic callus of the heat reclamation 628, the anther somatic embryo occurrence rate of the heat reclamation 628 of the rubber tree variety can be increased to 50% or above by utilizing the induction culture medium, and the cotyledon embryo occurrence rate can be increased to 20% or above.

The invention provides a fritillaria tissue culture medium, a preparation method, a fritillaria tissue culture method and application, and belongs to the technical field of plant tissue culture. It is found for the first time that low-concentration picloram can achieve efficient browning resistance of fritillary callus; compared with the prior art, the formula of the fritillaria tissue culture medium has the advantages that the browning resistance efficiency is remarkable, healthy proliferation of calluses is synchronously promoted, and the contradiction that'browning inhibition 'and'growth promotion' are difficult to consider at the same time in a traditional fritillaria tissue culture method is solved. The method is suitable for various fritillaria such as fritillaria unibiacteata, bulbus fritillariae cirrhosae and bulbus fritillariae thunbergii, and has universality and industrialization prospects.

The invention relates to a method for preparing 3, 4, 5, 6-tetrachloropicolinic acid from picloram solid residues. The method comprises the following steps: dissolving picloram solid residues which take 6-amino-3, 4, 5-trichloropicolinic acid and 4-amino-3, 5, 6-trichloropicolinic acid as main components as raw materials in 1, 4-dioxane or other solvents, adding concentrated nitric acid to carry out N-amino nitration, then adding thionyl chloride to carry out sealed heating chlorination, and carrying out post-treatment to obtain 3, 4, 5, 6-tetrachloropicolinic acid. According to the method, picloram solid residues are converted into production raw materials with practical application value, diazotization operation in a traditional method is avoided, and the method has the advantages of being safe and mild in reaction, easy to operate, green and economical.

The invention discloses a gene gun mediated sugarcane genetic transformation method and a special culture medium thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to carry out sugarcane genetic transformation. The sugarcane genetic transformation method comprises the following steps: inducing sugarcane leaf sheaths or tillering plants on a callus induction culture medium to obtain calluses, and bombarding the calluses together with DNA to be transformed and regeneration factors to realize sugarcane genetic transformation, the callus induction culture medium contains 2 mg / L of 2, 4-D, 0.5 g / L of acid hydrolysis casein, 0.5 g / L of inositol, 1.2 mg / L of copper sulfate pentahydrate, 3.5 g / L of plant gel, 5 [mu] M of silver nitrate, 200 mg / L of ascorbic acid and 2 mg / L of picloram, and the pH value is 5.8; and the regeneration factor is TamGRF4-GIF1 or SomGRF4-GIF1, and the regeneration factor is TamGRF4-GIF1. The sugarcane genetic transformation method and the special culture medium thereof can be used for sugarcane genetic transformation.

The invention discloses a fungicide and herbicide compound composition and application thereof. The compound composition comprises any one herbicide and any one fungicide. The herbicide is prepared from glyphosate, fluroxypyr, clopyralid, picloram, sulfometuron methyl, MCPA (2-methyl-4-chlorophenoxyacetic acid) and dicamba; the fungicide is prepared from mancozeb, tebuconazole and carbendazim. According to the invention, the fungicide and the herbicide are combined according to a specific ratio, so that a remarkable synergistic interaction effect is shown. During implementation, the fungicide is firstly applied to soil, then the herbicide is sprayed, the fresh weight inhibition rate of solidago canadensis can be increased to 90% or above, the regeneration inhibition rate exceeds 85%, and the dosage is reduced by 33%-50% compared with the dosage of a single herbicide. The compound composition can effectively overcome the drug resistance of target weeds and reduce the environmental load of chemical agents, has the advantages of lasting prevention and control effect, low application cost and safety to associated plants, and provides an innovative solution for treatment of invasive plants.