Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method
A technology of culture medium and differentiation medium, applied in the direction of plant cells and so on
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[0015] The present invention is described in further detail below in conjunction with embodiment:
[0016] This Cinnamomum camphora body embryo culture medium is mainly made up of MS basic medium or MS salts, MT vitamin, additional growth regulator, coagulant and nutrient agent etc. 1.2% agar, 3% sucrose as the nutrient. This medium also contains 170mg / L sodium dihydrogen phosphate monohydrate, 30mg / L adenine sulfate, 0.5g / L hydrolyzed casein, the pH value of the medium is 5.0-6.0, and the optimum value is 5.7. According to the different components and addition amounts of additional growth regulators, it is prepared into three kinds of medium for different stages of application: one is the medium for inducing somatic embryo differentiation, the other is the medium for somatic embryo proliferation and embryo differentiation, and the third is the medium for embryo rooting Medium.
[0017] In the preparation of three different stages of culture medium, the somatic embryo induct...
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