Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method

A technology of culture medium and differentiation medium, applied in the direction of plant cells and so on

Inactive Publication Date: 2009-05-13
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

Propagating the endangered Cinnamomum camphora quickly and in batches should become the top priority of forestry work. After searching, there is no method of using

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  • Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method

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Embodiment Construction

[0015] The present invention is described in further detail below in conjunction with embodiment:

[0016] This Cinnamomum camphora body embryo culture medium is mainly made up of MS basic medium or MS salts, MT vitamin, additional growth regulator, coagulant and nutrient agent etc. 1.2% agar, 3% sucrose as the nutrient. This medium also contains 170mg / L sodium dihydrogen phosphate monohydrate, 30mg / L adenine sulfate, 0.5g / L hydrolyzed casein, the pH value of the medium is 5.0-6.0, and the optimum value is 5.7. According to the different components and addition amounts of additional growth regulators, it is prepared into three kinds of medium for different stages of application: one is the medium for inducing somatic embryo differentiation, the other is the medium for somatic embryo proliferation and embryo differentiation, and the third is the medium for embryo rooting Medium.

[0017] In the preparation of three different stages of culture medium, the somatic embryo induct...

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Abstract

The invention discloses a Cinnamonum kanehirae Hayata somatic embryo culture medium, which contains MS as a basic culture medium or MS salts and MT vitamin, and also contains growth regulators such as BA, Picloram, ABA and NAA, 170mg/L sodium dihydrogen phosphate monohydrate,30mg/L adenine sulfate, 0.5g/L caseinhydrolysate, 1.2 percent agaragar, and 3 percent saccharose, wherein three culture media for somatic embryogenesis, somatic embryo proliferation and embryo differentiation, and embryo rooting are prepared respectively. The tissue culture and rapid propagation method of Cinnamonum kanehirae Hayata by using the culture media comprises the following four steps: 1. the acquisition and disinfection of an explant; 2. the induction of a somatic embryo; 3. the somatic embryo proliferation and embryo differentiation; and 4. root induction and transplanting. The invention initially creates a complete set of method for Cinnamonum kanehirae Hayata somatic embryos to reproduce seedlings, saves the endangered species resource, lays a foundation for further exploring the gene engineering of somatic embryos, broadening explant materials and so on, and provides adequate wood for the mass production of Antrodia camphorata with extremely high economic value.

Description

【Technical field】 [0001] The invention relates to a culture medium for somatic embryos of plants and a method for rapid propagation of tissue culture. 【Background technique】 [0002] Cinnamomum kanehirae Hayata, also known as camphor, camphor, Lauraceae (Lauraceae), camphor (Cinnamomum), is a unique Lauraceae plant in southern China. It is called cinnamomum because of its thick and solid tree shape. Cinnamomum camphor wood is fragrant, and it is a well-known material for architecture, furniture and carving crafts, especially for Buddha statue carving, which is favored by Buddhists. Cinnamomum camphora oil can be extracted from the whole plant. In addition, the parasitic fungi on the camphor tree are called camphor mushrooms or Antrodia camphorata. People believe that it has anti-cancer, physical and other effects, and its price is increasing day by day. Cinnamomum camphora was originally widely distributed in southern China, and it has its natural distribution from 200 me...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 林新春曾余力王晓芹黄丽春
Owner ZHEJIANG FORESTRY UNIVERSITY
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