Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method

A technology of culture medium and differentiation medium, which is applied in the direction of plant cells, etc.

Inactive Publication Date: 2011-02-09
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

Propagating the endangered Cinnamomum camphora quickly and in batches should become the top priority of forestry work. After searching, there is no method of using the immature seeds of Cinnamomum camphora as explants to prepare effective tissue culture medium to carry out rapid propagation of Cinnamomum camphora. to report

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  • Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method

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Embodiment Construction

[0015] The present invention will be described in further detail below in conjunction with embodiments:

[0016] The Cinnamomum camphora embryo culture medium is mainly composed of MS basic medium or MS salts, MT vitamins, additional growth regulators, coagulants and nutrients, etc. The coagulant and its optimal addition amount are selected to account for the total weight of the medium 1.2% agar, 3% sucrose is selected as the nutrient. This medium also contains 170 mg / L sodium dihydrogen phosphate monohydrate, 30 mg / L adenine sulfate, 0.5 g / L hydrolyzed casein, and the pH value of the medium is 5.0-6.0, the best value is 5.7. According to the different components and amount of the additional growth regulators, it is formulated into three different media for application: one is the medium for inducing somatic embryo differentiation, the other is the medium for somatic embryo proliferation and embryo differentiation, and the third is the embryo rooting. Medium.

[0017] In the prep...

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Abstract

The invention discloses a Cinnamonum kanehirae Hayata somatic embryo culture medium, which contains MS as a basic culture medium or MS salts and MT vitamin, and also contains growth regulators such as BA, Picloram, ABA and NAA, 170mg / L sodium dihydrogen phosphate monohydrate,30mg / L adenine sulfate, 0.5g / L caseinhydrolysate, 1.2 percent agaragar, and 3 percent saccharose, wherein three culture media for somatic embryogenesis, somatic embryo proliferation and embryo differentiation, and embryo rooting are prepared respectively. The tissue culture and rapid propagation method of Cinnamonum kanehirae Hayata by using the culture media comprises the following four steps: 1. the acquisition and disinfection of an explant; 2. the induction of a somatic embryo; 3. the somatic embryo proliferation and embryo differentiation; and 4. root induction and transplanting. The invention initially creates a complete set of method for Cinnamonum kanehirae Hayata somatic embryos to reproduce seedlings, saves the endangered species resource, lays a foundation for further exploring the gene engineering of somatic embryos, broadening explant materials and so on, and provides adequate wood for the mass production of Antrodia camphorata with extremely high economic value.

Description

【Technical Field】 [0001] The invention relates to a plant somatic embryo culture medium and a tissue culture and rapid propagation method. 【Background technique】 [0002] Cinnamomum kanehirae Hayata, also known as Cinnamomum camphora, Cinnamomum camphora, Lauraceae (Lauraceae), Cinnamomum (Cinnamomum), is a species of Cinnamomum endemic to southern China. It is called Cinnamomum camphora because of its thick and firm tree. Cinnamomum camphora wood is fragrant, and it is a famous material for construction, furniture and carving crafts, especially Buddha sculptures, which are favored by Buddhists. Cinnamomum camphora oil can be extracted from the whole plant. In addition, the parasitic fungus on the camphor tree is called Antrodia camphorata or Antrodia camphorata. Everyone believes that it has anti-cancer and strengthening effects, and its price is rising day by day. Cinnamomum cinnamomum was originally widely distributed in southern China, and has its natural distribution from...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 林新春曾余力王晓芹黄丽春
Owner ZHEJIANG FORESTRY UNIVERSITY
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