Cinnamomum kanahirai hay body embryo culture medium and tissue culture rapid propagation method
A technology of culture medium and differentiation medium, which is applied in the direction of plant cells, etc.
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[0015] The present invention will be described in further detail below in conjunction with embodiments:
[0016] The Cinnamomum camphora embryo culture medium is mainly composed of MS basic medium or MS salts, MT vitamins, additional growth regulators, coagulants and nutrients, etc. The coagulant and its optimal addition amount are selected to account for the total weight of the medium 1.2% agar, 3% sucrose is selected as the nutrient. This medium also contains 170 mg / L sodium dihydrogen phosphate monohydrate, 30 mg / L adenine sulfate, 0.5 g / L hydrolyzed casein, and the pH value of the medium is 5.0-6.0, the best value is 5.7. According to the different components and amount of the additional growth regulators, it is formulated into three different media for application: one is the medium for inducing somatic embryo differentiation, the other is the medium for somatic embryo proliferation and embryo differentiation, and the third is the embryo rooting. Medium.
[0017] In the prep...
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