208 results about "Ammonium glufosinate" patented technology

The invention discloses a method for detecting glufosinate-ammonium residues in coffee in dual modes of colorimetry and chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that Schiff base formed by 4-aminoantipyrine and glutaraldehyde serves as a complexing agent of Cu < 2 + >, and then a copper-doped carbon dot Cu-CDs nano-enzyme is prepared through microwave digestion; according to the present invention, the Cu-CDs nano-enzyme has excellent peroxidase-like activity, can oxidize the colorless 2, 2 '-biazo-bis-3-ethylbenzothiazoline-6-sulfonic acid into the blue-green oxidized ABTS, and can improve the chemiluminescence intensity of the reaction of N-(4-aminobutyl)-N-ethylisoluminol and H2O2 by nearly 100 times when the Cu-CDs nano-enzyme is used as the chemiluminescence catalyst; based on the inhibition effect of glufosinate-ammonium on the activity of Cu-CDs POD, a sensitive and rapid colorimetric and chemiluminescence two-channel GM detection technology is developed, and the method has potential application value in the aspect of coffee safety monitoring.

The invention relates to a nucleic acid molecule of a rice transformation event RN85zN-eH and a detection method. The nucleic acid molecule of the rice RN85zN-eH comprises a sequence shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The rice transformation event RN85zN-eH disclosed by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85zN-eH or not.

The invention discloses a glufosinate-ammonium production waste gas treatment device and method, and relates to the technical field of waste gas treatment.The glufosinate-ammonium production waste gas treatment device comprises an adsorption mechanism, an air pipeline and a heating decomposition mechanism, and the adsorption mechanism comprises an adsorption disc and a power structure driving the adsorption disc to rotate; the adsorption disc comprises a plurality of annularly distributed adsorption units; the air pipeline wraps one side of the adsorption disc to form an adsorption area, and the adsorption area is used for adsorbing and filtering pollutants in waste gas in the air pipeline; the heating decomposition mechanism wraps the other side of the adsorption disc to form a desorption area, and the desorption area is used for releasing the adsorbed pollutants from the adsorption unit, decomposing the adsorbed pollutants into harmless substances and recovering the activity of the adsorption unit; the adsorption disc is driven by the power structure to continuously rotate, so that the dynamic switching between the adsorption area and the desorption area is realized, and the continuous operation of waste gas treatment is realized. Dynamic switching between the adsorption area and the desorption area is achieved, pollutants are efficiently captured by adsorption particles in the adsorption unit, shutdown is not needed, and the requirement for continuous operation of glufosinate-ammonium production is met.

The invention relates to the technical field of pesticides and herbicides, in particular to a preparation method of L-refined glufosinate ammonium, which comprises the following steps: firstly, preparing an intermediate from diethylamine, diethyl methylphosphite, a compound # imgabs0 # as shown in a formula III and a second solvent in the presence of an iridium-containing Lewis base catalyst by adopting a one-pot method; and carrying out rearrangement and hydrolysis reaction to obtain the L-refined glufosinate-ammonium. The preparation method of the L-refined glufosinate-ammonium has the advantages of simple process steps, mild reaction conditions, high yield and high ee value.

The present disclosure relates to processes for the preparation of glufosinate-ammonium or derivatives thereof. In particular, the present disclosure relates to a process for the preparation of glufosinate-ammonium of formula (I) or a salt, enantiomer or a mixture of all proportions of enantiomers thereof, said process comprising preparing a compound of formula (III) from a compound of formula (II) or a salt, enantiomer or a mixture of all proportions of enantiomers thereof; and preparing glufosinate-ammonium as shown in the formula (I) or salts, enantiomers or a mixture of enantiomers in all proportions from the compound as shown in the formula (III). # imgabs0 #

The invention relates to the field of bioengineering, in particular to a mutant, an expression vector, a host and application thereof. The invention provides application of a mutation site as a target spot in preparation of alpha keto acid and / or L-glufosinate-ammonium. The mutation sites comprise one or more of the 52nd site, the 54th site, the 56th site, the 58th site, the 217th site, the 354th site and the 355th site of the D-amino acid oxidase. According to the invention, an active pocket of D-amino acid oxidase is modified, and a D-amino acid oxidase single-point or multi-point combined mutant with significantly enhanced catalytic activity on a catalytic substrate DL-glufosinate-ammonium is screened through multiple rounds of iterative saturated mutation. By utilizing the D-amino acid oxidase mutant provided by the invention, kinetic resolution of racemic glufosinate-ammonium is realized, and the D-amino acid oxidase mutant can be widely applied to production and application of L-glufosinate-ammonium.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

A D-amino acid oxidase mutant with significantly improved catalytic performance, a gene encoding the gene, a vector containing the gene, a genetically engineered bacterium, and the application of the said mutant in the microbial catalytic preparation of L-ammonium glufosinate. The D-amino acid oxidase mutant was obtained from the amino acid shown in SEQ ID NO.1 by single-point mutation or multi-point combined mutation. The beneficial effects are mainly reflected in the following: the D-amino acid oxidase mutant with improved enzyme activity and thermal stability can be used in the microbial catalysed preparation of L-ammonium glufosinate, which is conducive to industrial production and has a better application prospect.

The invention discloses a transaminase mutant and application of the transaminase mutant in asymmetric synthesis of L-glufosinate-ammonium. The transaminase mutant is obtained by carrying out single-point saturated mutation or iterative saturated mutation on 13th, 17th, 19th or 22th amino acids of an amino acid sequence shown as SEQ ID NO.1. The invention further discloses a preparation method of the transaminase mutant. The invention provides a novel high-activity transaminase mutant derived from salmonella, and the whole-cell relative enzyme activity of different mutants reaches 250.1%-397.9% compared with that before mutation.

The invention discloses a monocotyledon anti-glufosinate-ammonium herbicide gene, a protein, a method and application, the nucleotide sequence of the monocotyledon anti-glufosinate-ammonium herbicide gene is shown as SEQ ID NO.1, the mutation site of the gene is single base substitution, and the position of the mutation site is LOCOs04g56170 gene; the amino acid sequence coded by the nucleotide sequence SEQ ID NO. 1 is as shown in SEQ ID NO. 2. A new rice germplasm resistant to glufosinate-ammonium herbicide is obtained through experiments, by spraying glufosinate-ammonium, it is confirmed that rice containing the mutant protein has glufosinate-ammonium resistance after glufosinate-ammonium is applied, and the conservative property of the mutant protein is verified in a hybridization mode.

The invention relates to the field of molecular crop breeding, in particular to a construction body capable of effectively identifying a male sterile line of corn and application of the construction body. The functional element expression cassettes are respectively a report system Ruby expression cassette, a male fertility restoring gene MS45 expression cassette, a corn tassel pollen lethal gene ZmAmylase expression cassette and a glufosinate-resistant herbicide gene PAT expression cassette. According to the progeny plant seeds with the specific constructs obtained by the invention, under the condition of not using other equipment, the purple maintainer line plants can be directly observed by naked eyes, and the progeny plant seeds have herbicide glufosinate-ammonium resistance, so that the screening mode is greatly simplified, and a large amount of labor cost is saved; meanwhile, herbicide resistance is generated, and an important effect is achieved on field screening or weed killing.

The invention relates to a corn transformation event MN85zN-Ex nucleic acid molecule and a detection method. The corn transformation event MN85zN-Ex nucleic acid molecule comprises a sequence shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The corn transformation event MN85zN-Ex has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains the DNA molecule of the transgenic corn event MN85zN-Ex or not.

Provided is a method for synthesizing L-glufosinate, comprising the following steps: first making L-homoserine react with acetic acid to form a compound A or a salt thereof; then catalyzing the compound A or the salt thereof by a synthetase to form a compound B; then carrying out chlorination and ring opening on the compound B to form an intermediate compound C; then carrying out an Arbuzov reaction on the intermediate compound C and a phosphine reagent to form a compound D or a salt thereof; and carrying out a hydrolysis reaction on the compound D or the salt thereof regardless of whether the intermediate is separated or not to form L-glufosinate or a salt thereof. The method for synthesizing L-glufosinate combines a chemical synthesis method with an enzyme catalysis method to obtain L-glufosinate having both a high e.e. value and high yield, and has simple preparation steps and controllable cost.

The invention relates to a nucleic acid molecule of a rice transformation event RN85N-e3 and a detection method. The nucleic acid molecule of the rice transformation event RN85N-e3 comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 2. The rice transformation event RN85N-e3 disclosed by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85N-e3 or not.

The present application belongs to the field of pesticides, and particularly relates to a herbicidal composition containing a fused ring substituted aromatic compound and application thereof. The composition comprises a herbicidally effective amount of active ingredient A and active ingredient B, wherein the active ingredient A is or a salt / ester thereof; the active ingredient B is selected from one or more of the following compounds and salts / esters thereof: glyphosate, glufosinate-ammonium, glufosinate-P, paraquat, diquat, ACCase inhibitors, PPO inhibitors, etc. The composition can effectively control / eliminate grass weeds and broadleaf weeds, etc., and has the characteristics of expanding the herbicidal spectrum, reducing the application amount, being able to produce a synergistic effect and solving the problem of resistant weeds, etc.

According to the glufosinate-ammonium mother liquor decolorizing device provided by the invention, the glufosinate-ammonium mother liquor is decolorized by arranging the resin adsorption device, and the resin column in the resin adsorption device is filled with the resin, so that the glufosinate-ammonium mother liquor decolorizing device has the characteristics of large treatment capacity, easiness in regeneration and high cyclic utilization rate during decolorizing; the problems that the amount of generated hazardous waste is large and the hazardous waste is not easy to treat due to the fact that the usage amount is large and regeneration is not easy after adsorption saturation when activated carbon is adopted for decolorizing glufosinate-ammonium mother liquor wastewater in the prior art are solved. A first resin column and a second resin column in the resin adsorption device are connected with the mother liquor storage tank, the decolorizing mother liquor storage tank, the backflushing liquid storage tank and the solvent storage tank through a plurality of valves respectively, and the other resin column can continue to work when the first resin column or the second resin column is subjected to backflushing regeneration through control of the valves; and the continuous decoloring process is effectively ensured.

The invention discloses an optically active glufosinate-lysine salt with a structure as shown in a formula (I-IV) as shown in the specification. The invention also discloses a method for preparing the optically active glufosinate-lysine salt, which comprises the following steps: taking D, L-glufosinate or a salt thereof as a raw material, taking D-lysine or L-lysine as a resolving agent, and taking D-lysine or L-lysine as a resolving agent to obtain the optically active glufosinate-lysine salt. And obtaining the L-glufosinate-D-lysine salt, the D-glufosinate-D-lysine salt, the L-glufosinate-L-lysine salt or the D-glufosinate-L-lysine salt through a resolution reaction, crystallization and filtration in a suitable resolving agent system. The D-glufosinate-D-lysine salt or the L-glufosinate-L-lysine salt is prepared by taking the D-glufosinate-D-lysine salt as a resolution solvent of an anhydrous system; and the L-glufosinate-D-lysine salt or the D-glufosinate-L-lysine salt is prepared by using a resolution solvent as an aqueous system.

The present disclosure relates to a method for preparing glufosinate-ammonium or its derivatives.

The utility model relates to the technical field of glufosinate-ammonium impurity separation, and discloses a device for separating glufosinate-ammonium impurities through flotation, which comprises a separation box and a collection barrel, a support foot stand is arranged at the bottom of the separation box, and a bottom discharge pipe is arranged at the bottom of the support foot stand and is positioned in the middle of the support foot stand. According to the device for flotation separation of glufosinate-ammonium impurities, when a solution flows along the flow guide groove, liquid flowing in the flow guide groove is layered through specific gravity under the action of gravity, meanwhile, centrifugal force is generated when the liquid flows along the flow guide groove, the liquid is made to approach the outer side through the centrifugal force, and the liquid is made to approach the outer side; and through cooperation of gravity and centrifugal force, the flotation effect is improved, the liquid surface is collected layer by layer, the flotation separation precision is improved, and subsequent processing work can be conveniently conducted according to different contents of liquid at different layers.

A process for making L-glufosinate, the process comprising the following steps: a) reacting a compound according to formula (I), (I), wherein R1 is hydrogen, (C1-C12)-alkyl, (C1-C12)-haloalkyl, (C6-C10)-aryl, (C6-C10)-haloaryl, (C7-C10)-aralkyl, (C7-C10)-haloaralkyl, (C4-C10)-cycloalkyl, or (C4-C10)-halocycloalkyl, with a compound according to formula (II), (II), wherein R2 and R3 are independently from each other (C1-C8)-acyloxy, (C1-C8)-alkoxy or hydroxy, or R2 and R3 form together an epoxide group or an ethylene carbonate group, and optional subsequent acidic treatment to form a diol according to formula (III), (III), b) Oxidizing the diol according to formula (III) to form 2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid (PPO); and c) aminating the PPO to L-glufosinate.

The invention belongs to the technical field of pesticides, and discloses a fluoroglycofen-ethyl, quizalofop-p-ethyl and glufosinate-ammonium microemulsion as well as a preparation method and application thereof. The invention relates to a microemulsion containing fluoroglycofen-ethyl, quizalofop-p-ethyl and glufosinate-ammonium. Comprising the following components in percentage by weight: 0.5 to 2 percent of fluoroglycofen-ethyl, 1.0 to 5.0 percent of quizalofop-p-ethyl, 15 to 30 percent of glufosinate-ammonium, 0.5 to 10 percent of styryl polyoxyethylene ether phosphate monoester, 0.5 to 10 percent of styryl polyoxyethylene ether phosphate diester, 2 to 5 percent of fatty alcohol polyoxyethylene ether sodium sulfate, 15 to 20 percent of alkyl glycoside, 8 to 15 percent of solvent and the balance of deionized water. According to the microemulsion disclosed by the invention, by adding the styryl polyoxyethylene ether phosphate monoester and the styryl polyoxyethylene ether phosphate diester, the microemulsion can be stably stored at the temperature of-15 to 54 DEG C, and the influence of precipitation of effective components or a surfactant of a product on the quality of the product in extreme weather is avoided.

The invention discloses a kind of crystal formation of L glufosinate ammonium salt and preparation method thereof, the X-ray diffraction pattern of the crystal formation of L glufosinate ammonium salt includes 10.696 ± 0.2 °, 18.34 ± 0.2 °, 18.598 ± 0.2 °, 20.185 ± 0.2 °, 21.893 ± 0.2 ° and 32.428 ± 0.2 ° 2θ at least one characteristic peak, after testing, the crystal formation of this L glufosinate ammonium salt is stored 10 days at room temperature under humidity 65%, mass change is all below 0.5%, without obvious hygroscopicity, and crystal formation has no obvious change, which is conducive to it keeping dry during storage and use, avoids deterioration or activity reduction due to humidity. Meanwhile, the crystal formation performs well in stability test, and after long-term storage, crystal structure also does not change. This high stability ensures the lasting effectiveness of the crystal formation of L glufosinate ammonium salt in herbicidal drug products.

The invention relates to the technical field of pesticide production, and particularly discloses a preparation process of glufosinate-ammonium intermediate 3-(methyl hydroxyl phosphonyl) propionaldehyde. The preparation method comprises the following steps: 1, carrying out addition reaction on diethyl methylphosphonite and acrylic acid in absolute ethyl alcohol to generate 3-[ethyoxyl (methyl) phosphonyl] propionate; in the second step, 3-[ethyoxyl (methyl) phosphonyl] propionate is subjected to a hydrosilylation reaction in an oriented mode, and 3-triethyl siloxy-3-ethyoxyl propyl methyl ethyl phosphonate is generated; and 3, carrying out a simple dilute acid hydrolysis reaction to obtain the 3-(methyl hydroxyl phosphonyl) propionaldehyde. According to the method, a three-step reaction route of addition-hydrosilylation-hydrolysis is adopted, and the acrylic acid is used for replacing the traditional high-toxicity raw material acrolein, so that the toxicity risk is avoided from the source, the yield of the target glufosinate-ammonium intermediate is remarkably improved, the total yield of the three-step reaction can reach 95% or above, and the method is suitable for industrial production. And an efficient and green brand-new technical scheme is provided for large-scale production of glufosinate-ammonium.

The invention discloses a method for synthesizing L-glufosinate-ammonium by using whole cells as a catalyst. The method comprises the following step: carrying out cell catalytic reaction in a water phase system containing microbial cells, L-homoserine and / or activated L-homoserine and a phosphorus donor compound to prepare the L-glufosinate-ammonium. According to the present invention, the microbial cells are adopted as the catalyst, the L-homoserine and / or the activation form thereof are / is adopted as the raw material to directly synthesize the L-glufosinate-ammonium in the pure water system, and the method has advantages of high selectivity, high catalysis efficiency, good stability, cell recycling and the like.

The invention discloses a mutant of glutamate dehydrogenase derived from clostridium symbiosum, and an application of the mutant in synthesis of L-glufosinate-ammonium, and particularly discloses a mutant of glutamate dehydrogenase derived from clostridium symbiosum and an application of the mutant in synthesis of L-glufosinate-ammonium. According to the invention, single-site or multi-site mutation is carried out on wild-type glutamate dehydrogenase derived from clostridium symbiotic as shown in SEQ ID NO.1 by virtue of a mutation transformation method which is designed by carrying out rational analysis on amino acid residues in a binding pocket of glutamate dehydrogenase and a substrate, a geometrical shape in a molecular tunnel and interaction of domains in a structural domain; according to the obtained single-site or multi-site mutant, the catalytic activity to 2-carbonyl-4-(hydroxymethylphosphonyl) butyric acid is remarkably improved, and the mutant has an application prospect in preparation of L-glufosinate-ammonium through biological catalysis.

The invention relates to the technical field of pesticides, and particularly discloses a dispersible oil suspending agent containing refined glufosinate-ammonium and a preparation method of the dispersible oil suspending agent. The invention relates to a dispersible oil suspending agent containing refined glufosinate-ammonium. The dispersible oil suspending agent comprises the following raw materials in percentage: 10-15% of a compound of flumioxazin and refined glufosinate-ammonium, 10-15% of an emulsifier, 1-3% of a dispersing agent, 2-5% of a stabilizer, 0.3-0.8% of a thickening agent, 3-5% of an anti-settling agent, 1-5% of an anti-drifting agent and the balance of oily filler, the flumioxazin and the fine glufosinate-ammonium are adopted as main active pharmaceutical ingredients of the suspending agent, the two agents with different action mechanisms and different action modes can complement each other in advantages, the quick-acting property and the persistence of the agent are enhanced, meanwhile, the development of drug resistance of weeds can be effectively overcome or delayed, and the control effect is improved.

The application provides a genetic transformation method of sugarcane variety Liucheng 1541 and belongs to the technical field of plant genetic engineering. The genetic transformation method is that leaf tissues of the sugarcane variety Liucheng 1541 are induced and cultured on a callus induction culture medium containing 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine; the obtained embryogenic callus is infected by agrobacterium; after co-cultivation and recovery culture, the obtained recovered callus is transferred to a screening culture medium containing glufosinate ammonium for screening culture; then the screened resistant embryogenic callus is inoculated on a differentiation culture medium containing glufosinate ammonium for differentiation culture; and after rooting culture, the transgenic plant of Liucheng 1541 is obtained. The method improves the embryogenic callus induction rate, solves the problem of callus browning in the tissue culture process of the variety Liucheng 1541, determines the glufosinate ammonium screening concentration of the embryogenic callus, and establishes the genetic transformation system of Liucheng 1541.

The application discloses a preparation method of ammonium glufosinate, which comprises the following steps: adding diethyl malonate and liquid bromine into a first solvent to react to obtain intermediate I; adding the intermediate I, potassium phthalimide and a quaternary ammonium salt catalyst into toluene to react to obtain intermediate II; adding the intermediate II, potassium carbonate, 1,2-dibromoethane and a phase transfer catalyst into a second solvent to react to obtain intermediate III; adding the intermediate III and diethyl methyl phosphonite into toluene to react under argon to obtain intermediate IV; adding the intermediate IV into hydrochloric acid to react under reflux; cooling, precipitation, drying to obtain intermediate V; mixing the intermediate V with water, passing in ammonia and adjusting pH value; adding methanol and stirring to react; cooling, filtering and drying to obtain ammonium glufosinate. The method can avoid using sodium cyanide or hydrocyanic acid, and no large amount of difficult-to-separate ammonium chloride is generated, the content of the obtained ammonium glufosinate is 90% to 98%, and the total yield is greater than 68%.

The present invention relates to a nicotinic acid phosphoribosyltransferase mutant and a coding gene, and its application in the biosynthesis of L-phosphinothricin, D-amino acid, L-tert-leucine or L-aminobutyric acid by a multi-enzyme coupling method. The beneficial effects of the present invention are mainly reflected in: (1) the nicotinic acid phosphoribosyltransferase mutant of the present invention improves the intracellular NAD level, brings an efficient, low-cost and environmentally friendly production method for the coenzyme-dependent multi-enzyme catalytic reaction, and does not require the additional addition of coenzymes; (2) the nicotinic acid phosphoribosyltransferase mutant of the present invention has better catalytic efficiency, and when the intermediate product PPO is used as a substrate for catalytic reaction, the conversion rate is much higher than that of the original enzyme, and the L-phosphinothricin yield is also greatly improved; (3) the present invention can directly use PPO to prepare and produce L-phosphinothricin without exogenous addition of coenzymes, without the need for expensive resolution reagents, without the need for synthesizing phosphinothricin derivatives, and without the need for separation, re-racemization, and re-resolution of D-phosphinothricin.

The present invention relates to a process for the preparation of glufosinate comprising the steps of hydrolyzing an N-carbamoyl glufosinate amide with an amidase to form an N-carbamoyl amino acid compound followed by cleavage of the carbamoyl moiety of the N-carbamoyl amino acid compound.