106 results about "Ammonium glufosinate" patented technology

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

The present application belongs to the field of pesticides, and particularly relates to a herbicidal composition containing a fused ring substituted aromatic compound and application thereof. The composition comprises a herbicidally effective amount of active ingredient A and active ingredient B, wherein the active ingredient A is or a salt / ester thereof; the active ingredient B is selected from one or more of the following compounds and salts / esters thereof: glyphosate, glufosinate-ammonium, glufosinate-P, paraquat, diquat, ACCase inhibitors, PPO inhibitors, etc. The composition can effectively control / eliminate grass weeds and broadleaf weeds, etc., and has the characteristics of expanding the herbicidal spectrum, reducing the application amount, being able to produce a synergistic effect and solving the problem of resistant weeds, etc.

According to the glufosinate-ammonium mother liquor decolorizing device provided by the invention, the glufosinate-ammonium mother liquor is decolorized by arranging the resin adsorption device, and the resin column in the resin adsorption device is filled with the resin, so that the glufosinate-ammonium mother liquor decolorizing device has the characteristics of large treatment capacity, easiness in regeneration and high cyclic utilization rate during decolorizing; the problems that the amount of generated hazardous waste is large and the hazardous waste is not easy to treat due to the fact that the usage amount is large and regeneration is not easy after adsorption saturation when activated carbon is adopted for decolorizing glufosinate-ammonium mother liquor wastewater in the prior art are solved. A first resin column and a second resin column in the resin adsorption device are connected with the mother liquor storage tank, the decolorizing mother liquor storage tank, the backflushing liquid storage tank and the solvent storage tank through a plurality of valves respectively, and the other resin column can continue to work when the first resin column or the second resin column is subjected to backflushing regeneration through control of the valves; and the continuous decoloring process is effectively ensured.

A process for making L-glufosinate, the process comprising the following steps: a) reacting a compound according to formula (I), (I), wherein R1 is hydrogen, (C1-C12)-alkyl, (C1-C12)-haloalkyl, (C6-C10)-aryl, (C6-C10)-haloaryl, (C7-C10)-aralkyl, (C7-C10)-haloaralkyl, (C4-C10)-cycloalkyl, or (C4-C10)-halocycloalkyl, with a compound according to formula (II), (II), wherein R2 and R3 are independently from each other (C1-C8)-acyloxy, (C1-C8)-alkoxy or hydroxy, or R2 and R3 form together an epoxide group or an ethylene carbonate group, and optional subsequent acidic treatment to form a diol according to formula (III), (III), b) Oxidizing the diol according to formula (III) to form 2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid (PPO); and c) aminating the PPO to L-glufosinate.

The invention relates to the technical field of pesticide production, and particularly discloses a preparation process of glufosinate-ammonium intermediate 3-(methyl hydroxyl phosphonyl) propionaldehyde. The preparation method comprises the following steps: 1, carrying out addition reaction on diethyl methylphosphonite and acrylic acid in absolute ethyl alcohol to generate 3-[ethyoxyl (methyl) phosphonyl] propionate; in the second step, 3-[ethyoxyl (methyl) phosphonyl] propionate is subjected to a hydrosilylation reaction in an oriented mode, and 3-triethyl siloxy-3-ethyoxyl propyl methyl ethyl phosphonate is generated; and 3, carrying out a simple dilute acid hydrolysis reaction to obtain the 3-(methyl hydroxyl phosphonyl) propionaldehyde. According to the method, a three-step reaction route of addition-hydrosilylation-hydrolysis is adopted, and the acrylic acid is used for replacing the traditional high-toxicity raw material acrolein, so that the toxicity risk is avoided from the source, the yield of the target glufosinate-ammonium intermediate is remarkably improved, the total yield of the three-step reaction can reach 95% or above, and the method is suitable for industrial production. And an efficient and green brand-new technical scheme is provided for large-scale production of glufosinate-ammonium.

The invention discloses a method for synthesizing L-glufosinate-ammonium by using whole cells as a catalyst. The method comprises the following step: carrying out cell catalytic reaction in a water phase system containing microbial cells, L-homoserine and / or activated L-homoserine and a phosphorus donor compound to prepare the L-glufosinate-ammonium. According to the present invention, the microbial cells are adopted as the catalyst, the L-homoserine and / or the activation form thereof are / is adopted as the raw material to directly synthesize the L-glufosinate-ammonium in the pure water system, and the method has advantages of high selectivity, high catalysis efficiency, good stability, cell recycling and the like.

The invention relates to the technical field of pesticides, and particularly discloses a dispersible oil suspending agent containing refined glufosinate-ammonium and a preparation method of the dispersible oil suspending agent. The invention relates to a dispersible oil suspending agent containing refined glufosinate-ammonium. The dispersible oil suspending agent comprises the following raw materials in percentage: 10-15% of a compound of flumioxazin and refined glufosinate-ammonium, 10-15% of an emulsifier, 1-3% of a dispersing agent, 2-5% of a stabilizer, 0.3-0.8% of a thickening agent, 3-5% of an anti-settling agent, 1-5% of an anti-drifting agent and the balance of oily filler, the flumioxazin and the fine glufosinate-ammonium are adopted as main active pharmaceutical ingredients of the suspending agent, the two agents with different action mechanisms and different action modes can complement each other in advantages, the quick-acting property and the persistence of the agent are enhanced, meanwhile, the development of drug resistance of weeds can be effectively overcome or delayed, and the control effect is improved.

The application provides a genetic transformation method of sugarcane variety Liucheng 1541 and belongs to the technical field of plant genetic engineering. The genetic transformation method is that leaf tissues of the sugarcane variety Liucheng 1541 are induced and cultured on a callus induction culture medium containing 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine; the obtained embryogenic callus is infected by agrobacterium; after co-cultivation and recovery culture, the obtained recovered callus is transferred to a screening culture medium containing glufosinate ammonium for screening culture; then the screened resistant embryogenic callus is inoculated on a differentiation culture medium containing glufosinate ammonium for differentiation culture; and after rooting culture, the transgenic plant of Liucheng 1541 is obtained. The method improves the embryogenic callus induction rate, solves the problem of callus browning in the tissue culture process of the variety Liucheng 1541, determines the glufosinate ammonium screening concentration of the embryogenic callus, and establishes the genetic transformation system of Liucheng 1541.

The application discloses a preparation method of ammonium glufosinate, which comprises the following steps: adding diethyl malonate and liquid bromine into a first solvent to react to obtain intermediate I; adding the intermediate I, potassium phthalimide and a quaternary ammonium salt catalyst into toluene to react to obtain intermediate II; adding the intermediate II, potassium carbonate, 1,2-dibromoethane and a phase transfer catalyst into a second solvent to react to obtain intermediate III; adding the intermediate III and diethyl methyl phosphonite into toluene to react under argon to obtain intermediate IV; adding the intermediate IV into hydrochloric acid to react under reflux; cooling, precipitation, drying to obtain intermediate V; mixing the intermediate V with water, passing in ammonia and adjusting pH value; adding methanol and stirring to react; cooling, filtering and drying to obtain ammonium glufosinate. The method can avoid using sodium cyanide or hydrocyanic acid, and no large amount of difficult-to-separate ammonium chloride is generated, the content of the obtained ammonium glufosinate is 90% to 98%, and the total yield is greater than 68%.

The present invention relates to a process for the preparation of glufosinate comprising the steps of hydrolyzing an N-carbamoyl glufosinate amide with an amidase to form an N-carbamoyl amino acid compound followed by cleavage of the carbamoyl moiety of the N-carbamoyl amino acid compound.

Compositions and methods for the production of L-glufosinate are provided. The method involves converting racemic glufosinate to the L-glufosinate enantiomer or converting PPO to L-glufosinate in an efficient manner. In particular, the method involves the specific amination of PPO to L-glufosinate, using L-glutamate, racemic glutamate, or another amine source as an amine donor. PPO can be obtained by the oxidative deamination of D-glufosinate to PRO (2-oxo-4-(hydroxy (methyl) phosphinoyl) butyric acid) or generated via chemical synthesis. PPO is then converted to L-glufosinate using a transaminase in the presence of an amine donor. When the amine donor donates an amine to PPO. L-glufosinate and a reaction by product are formed. Because the PPO remaining represents a yield loss of L-glufosinate, it is desirable to minimize the amount of PPO remaining in the reaction mixture. Degradation, other chemical modification, extraction, sequestration, binding, or other methods to reduce the effective concentration of the by-product. i.e., the corresponding alpha ketoacid or ketone to the chosen amine donor will shift the reaction equilibrium toward L-glufosinate, thereby reducing the amount of PPO and increasing the yield of L-glufosinate. Therefore, the methods described herein involve the conversion or elimination of the alpha ketoacid or ketone by-product to another product to shift the equilibrium towards L-glufosinate.

The invention discloses a method for purifying refined glufosinate-ammonium, which belongs to the technical field of refined glufosinate-ammonium production, and is characterized in that during crystallization, a solvent is added into a crystallization system for multiple times at different temperatures so as to reduce the crystal nucleus generation speed in the crystallization process, and meanwhile, sufficient time is given to enable crystals to grow, so that the purity of the refined glufosinate-ammonium is improved. The refined glufosinate-ammonium hydrate solid with more regular refined glufosinate-ammonium crystals, higher crystallization rate and lower impurity content is obtained; ultrasonic waves are introduced in the refined glufosinate-ammonium crystallization process, on one hand, crystal nucleation can be promoted, the width of a crystallization metastable region is reduced, meanwhile, the particle size distribution of refined glufosinate-ammonium crystals can be narrowed, the efficiency during solid-liquid separation is higher, on the other hand, the ultrasonic waves can prevent crystal coalescence, the product has a better crystal form and higher product purity, and finally, the purity of the product is improved. The ultrasonic waves can provide energy to destroy hydrogen bonds of the refined glufosinate-ammonium crystalline hydrate, so that refined glufosinate-ammonium without crystal water is obtained, and the product purity is higher.

The invention provides a preparation method of refined glufosinate-ammonium salt soluble granules, which comprises the following steps: S1, crushing raw powder: crushing refined glufosinate-ammonium salt raw powder to obtain crushed raw powder; s2, jet milling: performing rotary cutting and drying on the crushed raw powder by using hot nitrogen, and performing cyclone separation to obtain dry powder; s3, raw material pretreatment: mixing the dry powder with a compound additive and water to form slurry, and adding a pH regulator to adjust the pH of the slurry; and S4, high-tower mixing granulation: atomizing the slurry through a nozzle at the top of a granulation tower, enabling the formed particles to freely fall to the bottom of the granulation tower, and carrying out whole-particle screening to obtain the refined glufosinate-ammonium salt soluble granules. The invention solves the problems of poor stability of active ingredients, strong hygroscopicity of particles, poor solubility, discontinuous production and the like in the prior art.

The present application relates to a kind of methods for preparing L-glufosinate ammonium salt by continuous bioconversion.The method comprises: (1) L-amino acid dehydrogenase wet bacteria and coenzyme regeneration enzyme wet bacteria are mixed, are treated by cell breaking, and solid bacterial residue is separated and removed, to obtain the aqueous solution containing L-amino acid dehydrogenase and coenzyme regeneration enzyme;(2) NAD + And raw material for NAD + / NADH cycle, to obtain biological catalyst solution;(3) PPO aqueous solution is reacted with ammonia solution, then the neutralization reaction liquid is reacted with the biological catalyst solution into microchannel reactor, to obtain the conversion liquid containing L-glufosinate ammonium salt.The present application realizes the continuous efficient conversion of PPO by cell breaking and impurity removing pretreatment and microchannel reaction, shortens reaction time, improves the space-time yield and conversion yield of L-glufosinate ammonium salt, and reduces the production of byproduct.

The invention discloses a mutant of glutamate dehydrogenase derived from clostridium symbiosum, and an application of the mutant in synthesis of L-glufosinate-ammonium, and particularly discloses a mutant of glutamate dehydrogenase derived from clostridium symbiosum and an application of the mutant in synthesis of L-glufosinate-ammonium. According to the invention, single-site or multi-site mutation is carried out on wild-type glutamate dehydrogenase derived from clostridium symbiotic as shown in SEQ ID NO.1 by virtue of a mutation transformation method which is designed by carrying out rational analysis on amino acid residues in a binding pocket of glutamate dehydrogenase and a substrate, a geometrical shape in a molecular tunnel and interaction of domains in a structural domain; according to the obtained single-site or multi-site mutant, the catalytic activity to 2-carbonyl-4-(hydroxymethylphosphonyl) butyric acid is remarkably improved, and the mutant has an application prospect in preparation of L-glufosinate-ammonium through biological catalysis.

The present application provides a kind of complex herbicide containing glufosinate-ammonium, which includes effective component and auxiliary material, and the effective component includes glufosinate-ammonium and fluroxypyr-methyl at a mass ratio of 0.5-10:1.The complex herbicide of the present application forms a mutual synergistic effect between glufosinate-ammonium and fluroxypyr-methyl, and the price of fluroxypyr-methyl is significantly lower than that of glufosinate-ammonium raw material.Compared with the same amount of glufosinate-ammonium herbicide, the complex herbicide of the present application can reduce the cost of drug use while improving the weed control effect, and can reduce the drug resistance of weeds to glufosinate-ammonium.The present application also optimizes the formula of different dosage forms, selects the auxiliary material and the components of the complex, uses fewer types of auxiliary materials while ensuring the efficacy of the preparation, and the auxiliary materials are commonly available and can further reduce the cost, so that the complex herbicide of the present application has higher market promotion value and economic benefits.

The invention provides a high-specificity rapid detection method and detector for chiral glufosinate-ammonium pesticide, and the method comprises the following steps: reacting a target solution with a target probe molecule with a target concentration in dimethyl sulfoxide, determining that the target solution is a chiral glufosinate-ammonium solution when a preset reaction requirement is met, and determining that the target solution is a chiral glufosinate-ammonium solution when the preset reaction requirement is met; collecting at least one first digital image after reaction under the target condition; extracting a first RGB value from the at least one first digital image; according to the first RGB value, the ratio between pixel intensity statistical values of the first target color channel is determined, and the pixel intensity statistical values of the first target color channel are pixel intensity statistical values of two color channels selected from an R channel, a G channel and a B channel; and inputting the ratio between the pixel intensity statistical values of the first target color channel into a pre-constructed concentration prediction model to obtain the predicted concentration of the chiral glufosinate-ammonium solution. By implementing the method, the requirements of high specificity and rapidness of chiral glufosinate-ammonium pesticide detection can be met.

The invention relates to the technical field of herbicides, and particularly discloses a microemulsion containing glufosinate-ammonium, quizalofop-p-ethyl and fluoroglycofen as well as a preparation method and application of the microemulsion. The invention discloses a microemulsion containing glufosinate-ammonium, quizalofop-p-ethyl and fluoroglycofen as well as a preparation method and application of the microemulsion. The microemulsion comprises the following raw materials in percentage by weight: 21-25% of effective active ingredients, 16-23% of an emulsifier, 6-9% of a dispersant, 18-22% of an organic solvent, 4-6% of suspension microcapsules and the balance of water, wherein the effective active components comprise glufosinate-ammonium, quizalofop-p-ethyl and fluoroethyl; in addition, the preparation method provided by the invention has the advantages that the herbicide is efficient and environment-friendly.

The invention belongs to the technical field of preparation of aluminum-containing compounds, and particularly discloses a method for preparing aluminum hydroxide with high specific surface area based on sodium tetrachloroaluminate. The preparation method comprises the following steps: mixing a sodium tetrachloroaluminate raw material (sodium tetrachloroaluminate solid waste residue as a byproduct in production of glufosinate-ammonium or methyl phosphorus dichloride) with water to obtain a mixed solution; mixing the mixed solution with alkali liquor, and reacting to obtain reaction liquid containing aluminum hydroxide; and sequentially aging, separating and washing to obtain the aluminum hydroxide with high specific surface area. The obtained aluminum hydroxide is a porous material, the specific surface area reaches 300 m < 2 > / g or above, the microstructure is a sheet / block nanoparticle combination, and the aluminum hydroxide has good dispersity and activity and can be used in the fields of catalyst carriers, adsorbents, flame retardants and the like. The method is simple in process and does not involve complex equipment and high energy consumption conditions; and when the treated object is industrial solid waste residue, the method has the advantages of high aluminum recovery rate, thorough impurity removal and no influence of phosphorus.

Methods relating to the preparation of glufosinate or its analogs and intermediates thereof. The methods include, a) reacting a compound of formula (II), an alcohol of formula (III), and a compound of formula (V), b) hydrolyzing the product of the above reaction to obtain glufosinate or its analog of formula (IV).

The invention provides a genetic transformation method of an excellent sugarcane variety Liucheng 1541, and belongs to the technical field of plant genetic engineering.The genetic transformation method comprises the steps that leaf tissues of the sugarcane variety Liucheng 1541 are taken and subjected to induction culture on a callus induction culture medium containing 2, 4-dichlorphenoxyacetic acid and 6-benzylaminopurine; the method comprises the following steps: infecting embryogenic calluses with agrobacterium, co-culturing, carrying out recovery culture, transferring the recovered calluses to a screening culture medium containing glufosinate-ammonium, carrying out screening culture, inoculating the screened resistant embryogenic calluses to a differential culture medium containing glufosinate-ammonium, carrying out differentiation culture, and carrying out rooting culture to obtain the glufosinate-ammonium resistant embryogenic callus. The transgenic plant of the Liucheng 1541 is obtained. According to the method, the embryogenic callus induction rate is increased, the problem of callus browning in the variety Liucheng 1541 tissue culture process is solved, the glufosinate-ammonium screening concentration of the embryogenic callus is determined, and a Liucheng 1541 genetic transformation system is determined.

The present application relates to a kind of D-amino acid oxidase mutant with significantly improved catalytic performance, coding gene, vector containing coding gene, genetically engineered bacteria, and the mutant is applied in the microbial catalytic preparation of L-glufosinate-ammonium.The D-amino acid oxidase mutant is obtained by single-point mutation or multiple-point combined mutation of the amino acid shown in SEQ ID NO.1.The beneficial effects of the present application mainly include: the present application provides D-amino acid oxidase mutant with improved enzyme activity and enzyme thermal stability, which can be used for microbial catalytic preparation of L-glufosinate-ammonium, conducive to industrial production, and has good application prospect.

The invention provides a preparation method and a preparation device of a phosphine aldehyde intermediate and glufosinate-ammonium. The preparation method comprises the following steps: mixing acrolein with an auxiliary agent, freezing at low temperature, settling and layering to obtain pretreated acrolein, mixing the pretreated acrolein with acetic acid to obtain a first mixed solution, mixing the first mixed solution with diethyl methylphosphite, performing condensation reaction to obtain acetal, and hydrolyzing the acetal to obtain the phosphine aldehyde intermediate. According to the method, acrolein is pretreated in a low-temperature freezing mode, side reactions can be effectively inhibited, and the product yield is increased. Furthermore, a circulation condensation reaction mode is adopted, the reaction heat is effectively diluted through circulation of a large amount of materials, the condensation reaction temperature is stabilized, and the reaction yield is increased. Experimental results show that according to the method provided by the invention, the yield of phosphine aldehyde is increased to 96%, and the yield of glufosinate-ammonium is increased to 88%.

The present disclosure relates to a preparation method for a glufosinate or a derivative thereof. In particular, the present disclosure relates to a preparation method for a glufosinate of formula (I) or a salt, an enantiomer or a mixture of all proportions of enantiomers thereof. The method comprises preparing a compound of formula (III) from a compound of formula (II) or a salt, an enantiomer or a mixture of all proportions of enantiomers thereof; and preparing a glufosinate of formula (I) or a salt, an enantiomer or a mixture of all proportions of enantiomers thereof from the compound of formula (III).

The invention relates to the technical field of bioengineering, in particular to a hydrogen peroxide-tolerant D-amino acid oxidase mutant as well as preparation and application thereof. Compared with an amino acid sequence of wild type D-amino acid oxidase, the D-amino acid oxidase mutant comprises one or more amino acids in the 52nd site, the 54th site, the 91th site, the 195th site, the 241th site and the 366th site which are mutated, and the mutated amino acids are selected from valine, asparagine, isoleucine or alanine; the amino acid sequence of the wild type D-amino acid oxidase is as shown in SEQ ID NO. 1. Compared with wild type D-amino acid oxidase, the D-amino acid oxidase mutant has the characteristics of high enzyme activity and high hydrogen peroxide tolerance; when racemic D, L-glufosinate-ammonium is used as a substrate for catalytic reaction, the conversion rate is far higher than that of a wild type, higher catalytic activity is achieved, and the PPO yield is also greatly improved. The invention further provides a process for preparing the L-glufosinate-ammonium through one-pot oxidation reduction, the inhibiting effect among multiple enzymes is achieved, and the catalytic efficiency reaches the optimal level of the industry.

The invention relates to a construction method of an Agrobacterium rhizogenes mediated acacia melanoxylon genetic transformation system, which comprises the following steps: by taking an acacia melanoxylon SR17 sterile single plant as an explant, selecting an Agrobacterium rhizogenes strain Ar1193, infecting for 45 minutes in a heavy suspension of which the bacterial liquid concentration OD600 is equal to 0.9 and the acetosyringone concentration is 100 [mu] mol.L <-1 >, co-culturing for 3 days, and then transferring into a bacteriostatic culture medium to induce hairy roots; and taking the transgenic hairy roots obtained by induction as explants, and inoculating the explants into a WPM culture medium containing 0.05 mg.L <-1 > TDZ and 200 mg.L <-1 > casein hydrolysate to induce regeneration of adventitious buds so as to obtain complete transgenic plants. The screening concentrations of the four antibiotics, namely kanamycin, hygromycin, glyphosate and glufosinate-ammonium, are determined; a stable and efficient acacia melanoxylon genetic transformation system is established, the hairy root induction rate reaches 63.17%, the transgenic positive rate reaches 15.42%, and the adventitious bud regeneration rate reaches 52%.

The invention relates to the technical field of pesticides and herbicides, and particularly discloses a synthesis process of an L-glufosinate-ammonium intermediate. According to the preparation method, dimethyl sulfoxide or N, N-dimethyl formamide is taken as the catalyst, so that the reaction rate of the key Arbuzov is remarkably increased, and the reaction time is greatly shortened. Meanwhile, triethylamine is introduced as an auxiliary agent and reacts with a byproduct chloroethane to generate tetraethylammonium chloride, so that the generation of impurities such as methyl ethyl phosphate ethyl ester is effectively inhibited, the purity of the intermediate (2S)-2-ethoxy carbonyl amino-4-ethoxy methyl phosphoryl-ethyl butyrate is remarkably improved, and the purity of the intermediate (2S)-2-ethoxy carbonyl amino-4-ethoxy methyl phosphoryl-ethyl butyrate is improved. And the diethyl methylphosphonite can be completely reacted without excessive feeding, so that the utilization rate of the raw materials is high. Besides, the process adopts a solvent-free reaction system, avoids the problems of cost increase, recovery burden, equipment utilization rate reduction and the like caused by solvent introduction, is suitable for industrial production, and has outstanding economic benefits and environmental benefits.

The invention discloses a glutamine synthetase (GS) mutant protein as well as a mutant gene and application thereof. According to the rice GS A2N mutant disclosed by the invention, the glufosinate-ammonium tolerance concentration of rice wild type GS can be improved by about 25 times while the enzyme activity is improved; according to the combined mutant rice GS A2N Y158C mutant, the glufosinate-ammonium tolerance concentration of rice wild type GS can be improved by about 150 times while the enzyme activity is improved by about 2 times, and transgenic rice can tolerate glufosinate-ammonium treatment with 4 times of field recommended dosage. After corresponding homologous sites are mutated, GS mutant transgenic plants of wheat, corn, soybean and rape can also tolerate treatment of glufosinate-ammonium with 4 times of recommended dosage in the field.

An enzymatic method for the production of an L-glufosinate P-alkyl ester can be performed. This method is characterized by reacting an L-glufosinate P-alkyl ester carbamoylate to give the corresponding L-glufosinate P-alkyl ester. This is catalyzed by a carbamoylase. The L-glufosinate P-alkyl ester carbamoylate employed may be obtained by a reaction from the corresponding L-glufosinate hydantoin P-alkyl ester. This is catalyzed by a hydantoinase, preferably an L-enantioselective hydantoinase. In a second aspect, an enzymatic method for enantioselective production of an L-glufosinate P-alkyl ester from a mixture MIIIA of L- and D-glufosinate P-alkyl ester hydantoins can be performed. The L-glufosinate P-alkyl ester obtained in the methods according to the first or second aspect of the invention may be saponified to give L-glufosinate.

The invention relates to the technical field of gene engineering, in particular to application of an EiABCG1 gene to improvement of glufosinate-ammonium resistance of rice. The nucleotide sequence of the EiABCG1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the EiABCG1 gene is as shown in SEQ ID NO: 2. Compared with a wild type ZH11 rice plant, the rice overexpressed by the EiABCG1-OE has the advantages that the glufosinate-ammonium resistance of the rice under the stress of glufosinate-ammonium is enhanced; the GR50 is obviously improved; the speed of discharging glufosinate-ammonium is improved; and the content of glufosinate-ammonium in the protoplast is obviously reduced. In conclusion, the EiABCG1 gene is a potential candidate new target for researching a glufosinate-ammonium-resistant mechanism.