39 results about "Glutamine synthetase" patented technology

The invention discloses a molybdenum oxide nanorod material, a preparation method of the molybdenum oxide nanorod material and application of the molybdenum oxide nanorod material to improvement of corn nutrient utilization, growth promotion and lodging resistance, and belongs to the technical field of corn nutrient utilization and agricultural nano material application. The morphology and dispersity of the nanorod are optimized by combining molecular composition characteristics of key enzymes (such as nitrate reductase and glutamine synthetase) in a corn nutrient utilization process, and finally the functional nanorod with targeting property and biocompatibility is obtained and can be widely applied to nutrient regulation and control scenes of corn water culture and field planting. After the material is applied, the activity of nitrate reductase and glutamine synthetase of a corn root system can be activated in a targeted manner, the nutrient utilization efficiency is accurately improved, and sufficient nutrition support is provided for plant growth. After the fertilizer is applied to plants, the root system is promoted to be developed, the stalks are thick and strong, the lodging resistance is remarkably enhanced, the protein content of the plants and grains can be increased, and the synergistic effects of efficient nutrient utilization, robust plant growth, lodging resistance and yield increase are achieved.

The present invention concerns a cell line comprising an endogenous dihydroorotate dehydrogenase (DHODH) and glutamine synthetase (GS) genes which are partially or fully inactivated, and its use for producing recombinant proteins.

The invention relates to a biomass energy utilization and resource utilization technology, and aims to provide a method for improving the carbon sequestration capability of nannochloropsis oculata by domesticating and regulating a key enzyme with high ammonium salt. The method comprises the following steps: carrying out nitrogen salt gradient domestication on nannochloropsis oculata cells, and regulating and controlling the expression quantity of five key enzymes, namely H < + > transport ATP enzyme, glutamine synthetase, asparagine synthetase, carbonic anhydrase and ribulose-1, 5-diphosphate carboxylase / oxygenase, in the nannochloropsis oculata cells; the domesticated and cultured nannochloropsis oculata can independently use coked ammonium sulfate as a nitrogen source required by growth; the domesticated algal strains are subjected to transfer culture, and verification and screening are carried out aiming at the capability of fixing CO2 in flue gas and the prevention of over-acidification of the culture medium. According to the method, the utilization of coked ammonium sulfate by the nannochloropsis oculata can be enhanced without causing over-acidification of the culture medium, the microalgae culture cost can be effectively saved, and the flue gas CO2 immobilization capability of the nannochloropsis oculata is improved; a new thought is provided for large-scale breeding and cultivation by using coked ammonium sulfate and improvement of economic efficiency.

The application discloses a novel double-carrier expression system for protein expression and a construction method and application thereof. The novel double-carrier expression system for protein expression comprises a first carrier containing a nucleotide sequence for coding a first functional domain of glutamine synthetase and a nucleotide sequence of light chain and heavy chain of a protein to be expressed; and a second carrier containing a nucleotide sequence for coding a second functional domain of glutamine synthetase and a nucleotide sequence of a ScFv sequence of the protein to be expressed, and an amino acid sequence of the glutamine synthetase is composed of the first functional domain and the second functional domain. By using the expression system, the transfection efficiency and screening efficiency are greatly improved, and the expression amount of the target protein is obviously increased.

The present invention relates to a strain which has increased glutamine productivity due to being transformed with a vector containing a nucleotide sequence that encodes a glutamine synthetase consisting of the amino acid sequence of SEQ ID NO: 1.

The invention discloses a fermentation production process of L-alanine, and relates to the technical field of biology. The fermentation production process comprises the following steps: carrying out seed culture on L-alanine producing bacteria to obtain a seed solution; inoculating the seed solution into a fermentation culture medium, and carrying out fermentation culture to prepare L-alanine; in the fermentation culture process, the pH range is controlled to be 6.9 + / -0.1. By regulating the pH value of the fermentation environment, the situation that excessive ammonium ions force cells to consume more ATP for nitrogen assimilation (such as a glutamine synthetase pathway) is effectively avoided, so that energy waste is reduced, and energy supply for synthesis of L-alanine is increased. The process not only improves the yield of the L-alanine, but also optimizes the energy utilization efficiency in the fermentation process, and provides reliable technical support for efficient and economic industrial production of the L-alanine.

The invention belongs to the technical field of gene engineering, and particularly relates to a glutamine synthetase mutant and application thereof in increasing the yield of ornamental blue. The invention discloses a glutamine synthetase mutant containing mutation sites E4K, Y192H and N205H for the first time, the optimal pH of the glutamine synthetase is successfully changed by modifying the glutamine synthetase, and the glutamine synthetase shows higher activity under neutral and slightly alkaline conditions, so that the accumulation amount of glutamine in cells is remarkably increased; when the mutant and the ornamental blue synthetase bpsA are co-expressed, a novel pH response domain synergistic double-enzyme system is constructed, so that the two enzymes can efficiently and cooperatively work under the same catalytic pH condition, and the optimized double-enzyme system not only improves the synthesis efficiency of glutamine, but also remarkably improves the yield of ornamental blue, and has a wide application prospect. And a more efficient solution is provided for biosynthesis of blue.

The present disclosure relates to an L-arginine-producing microorganism in which the activity of a regulator of nitrate reductase operon expression is attenuated and the activity of glutamine synthetase is enhanced, and to a method for producing L-arginine using same, wherein the L-arginine-producing microorganism, in which the activity of the regulator of nitrate reductase operon expression is attenuated and the activity of glutamine synthetase is enhanced, has been confirmed to exhibit increased L-arginine production capability compared to a parent strain, and thus can be widely used for L-arginine production.

A method for detection or monitoring status of traumatic brain injury (TBI) and / or spinal cord injury (SCI) in a subject is provided. In one embodiment, the method comprises contacting a specimen of bodily fluid obtained from the subject with reagents for assaying for a marker of TBI selected from aldolase C (ALDOC) and brain lipid binding protein (BLBP / FABP7), or a trauma-specific break down product (BDP) of ALDOC or BLBP / FABP7. The method further comprises measuring the amount of marker present in the specimen as compared to a control sample, and determining the presence of TBI or SCI when an elevated amount of marker is present in the specimen compared to the control sample. Optionally, the method further comprises measuring the amount of glutamine synthetase (GS), astrocytic phosphoprotein PEA-15 (PEA15), αB-crystallin (CRYAB / HSP27), a trauma-specific proteolytic cleavage product of ALDOC, GS, PEA15, or CRYAB, or any combination of two or more thereof.

Systems and methods for producing ammonia are described. In one embodiment, hydrogen, carbon dioxide, and nitrogen are dissolved in a solution. A glutamine synthetase inhibitor and autotrophic diazotroph bacteria are also placed in the solution.

The invention provides recombinant DNA molecules that are unique to maize event MON87429 and transgenic maize plants, maize plant parts, maize seeds, maize cells, and agricultural products containing maize event MON87429 as well as methods of using and detecting maize event MON87429. Transgenic maize plants containing maize event MON87429 exhibit tolerance to inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group, synthetic auxins, inhibitors of glutamine synthetase, and inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

The present invention provides the use of glutamine synthetase and its gene, BnaGLN1;2a, in nitrogen-efficient rapeseed breeding. Glutamine synthetase and related biomaterials can be used in any of the following ways: improving nitrogen absorption and / or utilization efficiency in rapeseed; preparing products that improve nitrogen absorption and / or utilization efficiency in rapeseed; increasing the nitrogen content of rapeseed; preparing products that improve and regulate the nitrogen content of rapeseed; increasing the biomass and / or yield of rapeseed; and preparing products that increase the biomass and / or yield of rapeseed.

The invention provides a notopterygium root complex microbial inoculant with excellent shape and high quality and a use method thereof, and belongs to the technical field of plant cultivation. The invention provides a complex microbial inoculant for inhibiting the formation of lateral roots of notopterygium seedlings, which comprises Witzia ciliaris and Brevibacillus laterosporus. The complex microbial inoculant is mainly used for reducing the content of 1-aminocyclopropane-1-carboxylic acid (on the premise of ethylene synthesis) and the content of ICA (Indoleacetic Acid) (auxin) by increasing the content of salicylic acid; the activity of peroxidase and glutamine synthetase and the content of total phenols in plants are improved; the contents of soil urease, polyphenol oxidase and alkali-hydrolyzable nitrogen are increased, and the contents of rapidly available potassium and total phenolic acid are reduced; and ammonium nitrogen is promoted to be converted into nitrate nitrogen, so that the generation of lateral roots of the notopterygium roots is inhibited, the nitrogen content of seedlings of the notopterygium roots is increased, the disease resistance is improved, and the breeding of excellent-shape and high-quality seedlings is promoted.

The invention discloses an application of a glutamine synthetase inhibitor in preparation of antitumor drugs, and belongs to the field of biological medicines. The CRISPR-Cas9 technology is used for knocking out glutamine synthetase (sgRNA), compared with shRNA, permanent gene knockout can be introduced, and the CRISPR-Cas9 technology has the potential of serving as a medicine for treating and resisting colorectal cancer. Glutamine synthetase is targeted for treatment on the basis of cancer chemotherapy drugs, the sensitivity of tumor cells to the chemotherapy drugs is remarkably improved, and the cell survival rate is reduced. The combined treatment of the targeted glutamine synthetase and the alphaPD-L1 neutralizing antibody can obviously enhance the anti-tumor immune response and improve the treatment efficiency.

A method for detection or monitoring status of traumatic brain injury (TBI) and / or spinal cord injury (SCI) in a subject is provided. In one embodiment, the method comprises contacting a specimen of bodily fluid obtained from the subject with reagents for assaying for a marker of TBI selected from aldolase C (ALDOC) and brain lipid binding protein (BLBP / FABP7), or a trauma-specific break down product (BDP) of ALDOC or BLBP / FABP7. The method further comprises measuring the amount of marker present in the specimen as compared to a control sample, and determining the presence of TBI or SCI when an elevated amount of marker is present in the specimen compared to the control sample. Optionally, the method further comprises measuring the amount of glutamine synthetase (GS), astrocytic phosphoprotein PEA-15 (PEA15), αB-crystallin (CRYAB / HSP27), a trauma-specific proteolytic cleavage product of ALDOC, GS, PEA15, or CRYAB, or any combination of two or more thereof.

The invention discloses a glutamine synthetase (GS) mutant protein as well as a mutant gene and application thereof. According to the rice GS A2N mutant disclosed by the invention, the glufosinate-ammonium tolerance concentration of rice wild type GS can be improved by about 25 times while the enzyme activity is improved; according to the combined mutant rice GS A2N Y158C mutant, the glufosinate-ammonium tolerance concentration of rice wild type GS can be improved by about 150 times while the enzyme activity is improved by about 2 times, and transgenic rice can tolerate glufosinate-ammonium treatment with 4 times of field recommended dosage. After corresponding homologous sites are mutated, GS mutant transgenic plants of wheat, corn, soybean and rape can also tolerate treatment of glufosinate-ammonium with 4 times of recommended dosage in the field.

The invention discloses an actinidia arguta endophytic bacterium R3 and application thereof in promoting plant growth, relates to the fields of bioinformatics, agronomy and microbiology, and in particular relates to an actinidia arguta endophytic bacterium R3 and application thereof in promoting plant growth by combining a classical microorganism isolation culture method for sampling field environmental factors and root endophytic bacterium community structures of actinidia arguta. The actinidia arguta endophytic bacterium R3 capable of promoting plant growth is screened out. The actinidia arguta endophytic bacteria R3 can obviously increase the total chlorophyll content, the glutamine synthetase enzyme activity and the auxin content of the corn, and can obviously increase the plant height, the root length and the biomass of the corn.

The invention provides application of geranyl linalool to inhibition of growth of lateral roots of notopterygium incisum seedlings and / or breeding of notopterygium incisum superior seedlings, and belongs to the technical field of planting of wild notopterygium incisum seedlings. In the invention, the geranyl linalool can promote the elongation of the roots of the notopterygium incisum seedlings and inhibit the formation of lateral roots by increasing the content of auxin in the notopterygium incisum seedlings and reducing the contents of jasmonic acid, abscisic acid and 1-aminocyclopropane-1-carboxylic acid (an ethylene synthesis precursor). Moreover, the geranyl linalool can also improve the activity of polyphenol oxidase and urease in rhizosphere soil, improve the activity of nitrate reductase and glutamine synthetase in leaves, improve the content of alkali-hydrolyzable nitrogen in soil, reduce the content of total phenolic acid in soil and promote conversion of ammonium nitrogen into nitrate nitrogen, so that nitrogen absorption and utilization are promoted, and growth and shape optimization of seedlings are facilitated. In addition, the geranyl linalool can also improve the abundance of rhizosphere soil probiotic bacteria and reduce the abundance of rhizosphere soil pathogenic fungi, so that the disease resistance of the notopterygium incisum seedlings is improved.

Glutamine synthetase mutant having glufosinate ammonium resistance, application thereof and a cultivation method therefor. Comparing with the reference sequence, the amino acid sequence of the glutamine synthetase mutant has one or a combination of the following mutations: (1) the amino acid of the glutamine synthetase mutant corresponding to amino acid site 59 of the reference sequence is mutated to X1, wherein X1=A, C, D, E, F, G, H, I, K, P, T, V or Y; (2) the amino acid of the glutamine synthetase mutant corresponding to amino acid site 296 of the reference sequence is mutated to X2, wherein X2=A, D, E, G, I, K, M, P, Q, R, S, T, or V.

A method for ameliorating a disease caused by nitration of tyrosine in protein includes administering a composition comprising tyrosine or a salt thereof to a subject in need thereof. Tyrosine as effective component of the present invention not only can enhance the activity of glutamine synthetase having reduced activity and but also has an effect of restoring the amount (i.e., ratio) of glutamine and glutamic acid in brain and the amount of ammonia to normal level, an effect of enhancing the insulin sensitivity in a model of type 2 diabetes, an effect of suppressing the excitotoxicity and oxidative stress in a model of epileptic seizure, an effect of reducing cerebral infarction and enhancing the activity of GS in a model of brain stroke, an effect of eliminating the nitration of tyrosine using human recombinant MnSOD, and an effect for acute renal failure and hyperammonemia.

The invention relates to a modified mammalian glutamine synthetase comprising a mutation at amino acid position 10 and / or 298 in a mammalian glutamine synthetase, wherein the mutation is selected from the group consisting of R298K, N10S, N10T and N10Q and to an expression vector, a nucleic acid and a eukaryotic host cell encoding said modified mammalian glutamine synthetase and to its use as a selection marker. The invention further relates to methods for preparing stable cell lines, or for producing a protein of interest using said modified mammalian glutamine synthetase for selection.

To provide a method for producing a human embryonic kidney (HEK) cell line that does not express functional endogenous dihydrofolate reductase (DHFR) and does not express functional endogenous glutamine synthetase (GS).SOLUTION: The present invention provides a method comprising the steps of: (a) mutating or knocking out the endogenous DHFR gene and the GS gene in a HEK cell line; (b) stably or transiently transfecting the HEK cell line with an AAV vector genome comprising a first heterologous nucleic acid sequence encoding a therapeutic protein or an inhibitory nucleic acid sequence and a first selectable marker; and (c) stably or transiently transfecting the HEK cell line with a second heterologous nucleic acid sequence and a second selectable marker.SELECTED DRAWING: Figure 2

A transgenic soybean event, Gm_CSM63714, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63714 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63714, and methods of using and detecting Gm_CSM63714 are also provided. Soybean plants containing the event Gm_CSM63714 exhibit tolerance to benzoic acid auxins such as dicamba; phenoxy auxins such as 2,4-D; inhibitors of glutamine synthetase such as glufosinate; and P-triketone inhibitors of 4-hydroxyphenylpyruvate dioxygenase (HPPD) such as mesotrione.

A herbicidal composition and a method for controlling weeds, including one or more uracil compounds of formula (I) and a compound of formula (II), and a herbicide compound of group B and a safener of group C. A weight ratio of the uracil compounds to the herbicide compound group B and the safener group C is 1:0.02 to 1:50, and the herbicide compound of group B is one or more of compounds B-1 to B-12: B-1, acetolactate synthase inhibitors; B-2, acetyl CoA carboxylase inhibitors; B-3, protoporphyrinogen IX oxidase inhibitors; B-4, 4-hydrophenylpyrubic acid dioxygenase inhibitors; B-5, phytoene desaturase inhibitors; B-6, photosystem II inhibitors; B-7, very-long-chain fatty acid synthesis inhibitors; B-8, microtubule formation inhibitors; B-9, auxin-type herbicides; B-10, enolpyruvylshikimate-3-phosphate synthase inhibitors; B-11, glutamine synthase inhibitors; and B-12, other herbicides; or agriculturally acceptable salts or derivatives thereof

The application discloses application of glutamine synthetase inhibitors in preparation of antitumor drugs and belongs to the field of biological medicines. The CRISPR-Cas9 technology is used to knock out glutamine synthetase (sgRNA), compared with shRNA, permanent gene knockout can be introduced, and the application has the potential as a drug for treating colorectal cancer. The application targets glutamine synthetase for treatment on the basis of cancer chemotherapy drugs, significantly improves the sensitivity of tumor cells to chemotherapy drugs, and reduces the cell survival rate. The application of the targeted glutamine synthetase and the neutralizing antibody of alphaPD-L1 combined treatment can significantly enhance the antitumor immune response and improve the treatment efficiency.

The present invention relates to the use of glutamine synthetase as a protein therapy (such as enzyme replacement protein therapy) for the treatment of hyperammonemia. In particular the invention relates to the systemic administration of glutamine synthetase. The glutamine synthetase may be provided in conjugated or fusion form, to increase its half-life in the circulation. Also provided is a pharmaceutical composition comprising glutamine synthetase. The invention also relates to the uses, methods, and compositions involving a combination of the glutamine synthetase protein and an ammonia lowering agent, such as a nitrogen scavenger.

The present invention relates to a microorganism belonging to the genus Corynebacterium having improved L-citrulline or L-arginine production capacity and a method for producing L-citrulline or L-arginine using the same, the microorganism belonging to the genus Corynebacterium having improved L-citrulline or L-arginine production capacity, the activity of glutamine synthetase, aspartate transaminase, and carbamyl phosphate synthetase being enhanced, so that the L-citrulline or L-arginine production capacity can be increased compared to the prior art, and a method for producing L-citrulline or L-arginine production capacity can be improved. And the production yield of the L-citrulline or the L-arginine can be improved.

The present application relates to recombinant expression of proteins within cells, including expression of therapeutic molecules. In some aspects, the present application relates to an intein-based split biomarker selection system allows using a single selection pressure for the incorporation of multiple transgenic fragments simultaneously.

Provided are compositions comprising polynucleotides encoding glutamine synthetase (GS) polypeptides having improved properties, such as increased enzymatic activity and / or increased thermostability. Also provided are recombinant DNA constructs, plants, plant cells, seed, grain comprising the polynucleotides. Additionally, various methods of employing the polynucleotides in plants, such as methods for increasing GS activity in a plant, methods for increase seed protein content in a seed of a plant, and methods for increasing yield of a plant, are also provided herein.